Short-term administration of common anesthetics does not dramatically change the endogenous peptide profile in the rat pituitary

Abstract

Cell-cell signaling peptides (e.g., peptide hormones, neuropeptides) are among the largest class of cellular transmitters and regulate a variety of physiological processes. To identify and quantify the relative abundances of cell-cell signaling peptides in different physiological states, LC-MS-based peptidomics workflows are commonly utilized on freshly dissected tissue. In such animal experiments, the administration of general anesthetics is an important step for many research projects. However, acute anesthetic administration may rapidly change the measured abundance of transmitter molecules and metabolites, especially in the brain and endocrine system, which would confound experimental results. The aim of this study was to evaluate the effect of short-term (< 5 min) anesthetic administration on the measured abundance of cell-cell signaling peptides, as evaluated by a typical peptidomics workflow. To accomplish this goal, we compared endogenous peptide abundances in the rat pituitary following administration of 5% isoflurane, 200 mg/kg sodium pentobarbital, or no anesthetic administration. Label-free peptidomics analysis demonstrated that acute use of isoflurane changed the levels of a small number of peptides, primarily degradation products of the hormone somatotropin, but did not influence the levels of most other peptide hormones. Acute use of sodium pentobarbital had a negligible impact on the relative abundance of all measured peptides. Overall, our results suggest that anesthetics used in pituitary peptidomics studies do not dramatically confound observed results.

Code

suppressPackageStartupMessages(c(
  library(tidyverse),
  library(knitr),
  library(statTarget),
  library(impute),
  library(cowplot),
  library(proBatch),
  library(pcaMethods),
  library(pROC)
  ))
FDR <- 0.05
LOG2FC <- 0.6
# get start time
start_time <- Sys.time()

Project and data background

In this study, we applied a non-targeted LC–MS and LC–MS/MS-based peptidomics approach to pituitary and hypothalamus tissue peptide extract collected from rats went under “No Anesthesia”, “5% Isoflurane” and “200 mg/kg Sodium Pentobarbital” anesthesia to explore differences in the profile of peptides caused by short-term anesthetics.

Rats were separated into “No Anesthesia”, “5% Isoflurane”, or “200 mg/kg Sodium Pentobarbital” groups. For each group, animals were subjected to the indicated condition prior to rapid decapitation and pituitary isolation, after which pituitaries were flash frozen. Peptides were then extracted from pituitary samples and analyzed using an LC-MS and LC-MS/MS peptidomics workflow.

Label-free peptidomics

Total umber of samples after LC-MS: 32

• No: 10 samples

• Iso: 11 samples

• SP: 11 samples

Total number of feature provided: 237

Three step data processing:

• Batch correction

• Log2 transformation

• EigenMS normalization

Batch correction

Batch correction using package statTarget evaluates the missing values and a feature will be kept if it has non-zero value for at least 50% of samples (statTarget default is 80%) in any one group (remove 71 features). It then imputes missing values for the present features and QC-based signal correction. At last, it removes features CV% > 50% (default, removes 2 features).

Code

pheno <- "revision_meta4samples.csv"
dfile = "revision_data4samples.csv"

statTarget::shiftCor(pheno, dfile,  QCspan = 0.25, Frule = 0.5,
                     degree = 2,imputeM = "KNN", ntree=500, coCV = 50)

statTarget: Signal Correction Start... Time: Thu Sep 21 14:38:41 2023 

* Step 1: Data File Checking Start..., Time:  Thu Sep 21 14:38:41 2023 

 Data Link 
 metaFile: revision_meta4samples.csv 
 profileFile: revision_data4samples.csv 

 45 Meta Samples vs 45 Profile samples
 The Meta samples list (*NA, missing data from the Profile File)

 [1] "20211105_QC1" "20211105_QC2" "Pituitary_08" "Pituitary_02" "Pituitary_19"
 [6] "Pituitary_29" "20211105_QC3" "Pituitary_04" "Pituitary_11" "Pituitary_34"
[11] "Pituitary_06" "20211105_QC4" "20211107_QC1" "Pituitary_18" "Pituitary_16"
[16] "Pituitary_31" "Pituitary_36" "Pituitary_07" "20211107_QC2" "Pituitary_22"
[21] "Pituitary_27" "Pituitary_25" "Pituitary_32" "Pituitary_30" "20211107_QC3"
[26] "20211108_QC1" "Pituitary_35" "Pituitary_09" "Pituitary_05" "Pituitary_10"
[31] "20211108_QC2" "Pituitary_12" "Pituitary_13" "Pituitary_28" "20211108_QC4"
[36] "20211108_QC5" "20211110_QC1" "Pituitary_03" "Pituitary_21" "Pituitary_26"
[41] "Pituitary_15" "Pituitary_24" "Pituitary_17" "Pituitary_23" "20211110_QC2"

 Meta-information: 

  Class No.
1   Iso  11
2    No  10
3    QC  13
4    SP  11
  Batch No.
1     1  12
2     2  13
3     3  11
4     4   9

 Metabolic profile information: 

               no.
QC and samples  45
Metabolites    237

* Step 2: Evaluation of Missing Value... 

 The number of missing value before QC based signal correction:  2174
 The number of filtered variables using the modified  50 % rule :   71 

* Step 3: Imputation start... 

 The imputation method was set at 'KNN'
 The number of missing value after imputation:  0
 Imputation Finished! 
 
* Step 4: QC-based Signal Correction Start... Time:  Thu Sep 21 14:38:41 2023 

 The Signal Correction method was set at QC-RFSC 

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 Calculation of CV distribution of raw peaks (QC)...

             CV<5%    CV<10%   CV<15%   CV<20%   CV<25%   CV<30%   CV<35%
Batch_1  4.8192771 19.879518 42.16867 59.63855 69.87952 81.32530 87.95181
Batch_2 14.4578313 32.530120 51.20482 71.68675 81.92771 86.14458 88.55422
Batch_3  7.8313253 22.891566 43.97590 59.63855 69.27711 77.71084 83.73494
Batch_4 22.2891566 39.156627 48.79518 61.44578 66.86747 72.28916 78.31325
Total    0.6024096  6.024096 17.46988 36.14458 48.79518 63.85542 71.68675
          CV<40%   CV<45%   CV<50%   CV<55%   CV<60%   CV<65%    CV<70%
Batch_1 92.16867 95.78313 96.98795 97.59036 98.79518 99.39759 100.00000
Batch_2 90.36145 93.97590 95.78313 95.78313 96.38554 96.98795  96.98795
Batch_3 87.34940 89.15663 91.56627 93.37349 95.78313 97.59036  99.39759
Batch_4 82.53012 83.73494 85.54217 87.34940 87.34940 91.56627  93.37349
Total   78.31325 82.53012 84.93976 86.74699 87.95181 92.16867  92.16867
           CV<75%    CV<80%    CV<85%    CV<90%    CV<95%   CV<100%
Batch_1 100.00000 100.00000 100.00000 100.00000 100.00000 100.00000
Batch_2  98.19277  98.79518  98.79518  98.79518  98.79518  98.79518
Batch_3  99.39759  99.39759  99.39759  99.39759  99.39759 100.00000
Batch_4  93.37349  95.18072  96.38554  97.59036  98.19277  98.19277
Total    94.57831  96.38554  97.59036  98.19277  98.79518  99.39759

 Calculation of CV distribution of corrected peaks (QC)...

            CV<5%   CV<10%   CV<15%   CV<20%   CV<25%   CV<30%   CV<35%
Batch_1 13.253012 46.98795 66.86747 81.32530 90.36145 95.18072 96.98795
Batch_2 16.265060 50.00000 74.69880 84.33735 92.16867 93.37349 95.78313
Batch_3 13.253012 43.37349 68.67470 80.72289 87.34940 90.36145 93.37349
Batch_4 27.710843 49.39759 64.45783 70.48193 76.50602 81.92771 83.73494
Total    4.819277 34.33735 62.65060 78.91566 89.15663 92.77108 96.38554
          CV<40%   CV<45%   CV<50%   CV<55%    CV<60%    CV<65%    CV<70%
Batch_1 98.19277 99.39759 99.39759 99.39759 100.00000 100.00000 100.00000
Batch_2 96.98795 96.98795 96.98795 97.59036  98.19277  98.19277  98.79518
Batch_3 94.57831 95.18072 96.38554 97.59036  99.39759  99.39759 100.00000
Batch_4 84.93976 87.34940 89.15663 89.15663  91.56627  94.57831  95.18072
Total   97.59036 98.19277 98.79518 99.39759 100.00000 100.00000 100.00000
           CV<75%    CV<80%    CV<85%    CV<90%    CV<95%   CV<100%
Batch_1 100.00000 100.00000 100.00000 100.00000 100.00000 100.00000
Batch_2  99.39759  99.39759  99.39759  99.39759  99.39759  99.39759
Batch_3 100.00000 100.00000 100.00000 100.00000 100.00000 100.00000
Batch_4  95.78313  95.78313  97.59036  98.19277  98.79518  98.79518
Total   100.00000 100.00000 100.00000 100.00000 100.00000 100.00000

* Step 5:  Removal of the features (CV% > 50%) Thu Sep 21 14:38:43 2023 

 No. of removed features: 2
 Feature name: FPAMPLSSLFANAVLRAQHLHQLAA SVFQELGKLTGPSNQ 

 Output Link: C:/Users/qiuha/OneDrive/projects/MaxHub/projects/2022-09-short-term-anesthetics 

 Correction Finished! Time:  Thu Sep 21 14:38:43 2023 

 #################################### 
 # Software Version: statTarget 2.0 + # 
 # Email: luanhm@sustech.edu.cn# 
 #################################### 

Code

fh = read.csv(file = "statTarget/shiftCor/After_shiftCor/shift_sample_cor.csv",header = TRUE, row.names = 1, stringsAsFactors = FALSE, sep = ",") %>%
        t(.) %>%
        as.data.frame()
labels_d1 <- as.matrix(fh[1,])
N_feature = nrow(fh[-1,])

# change row annotation for heatmap
fh_mod = read.csv(file = "statTarget/shiftCor/After_shiftCor/shift_sample_cor_mod.csv",header = TRUE, row.names = 1, stringsAsFactors = FALSE, sep = ",") %>%
        t(.) %>%
        as.data.frame()

feature_name = as.matrix(fh_mod[-1, "sample"]) %>%
        `rownames<-`(rownames(fh_mod)[-1]) %>%
        `colnames<-`("name") %>%
        as.data.frame() %>%
        rownames_to_column(., var = "variable")

• Features = 164

Data preview (after batch correction)

Code

#rownames(fh)[-1] == feature_name$variable
rownames(fh)[-1] = feature_name$name
# display table
fh %>% 
        knitr::kable(., digits = 3, "html") %>%
        kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
        kableExtra::scroll_box(width = "100%", height = "500px")

	Pituitary_08	Pituitary_02	Pituitary_19	Pituitary_29	Pituitary_04	Pituitary_11	Pituitary_34	Pituitary_06	Pituitary_18	Pituitary_16	Pituitary_31	Pituitary_36	Pituitary_07	Pituitary_22	Pituitary_27	Pituitary_25	Pituitary_32	Pituitary_30	Pituitary_35	Pituitary_09	Pituitary_05	Pituitary_10	Pituitary_12	Pituitary_13	Pituitary_28	Pituitary_03	Pituitary_21	Pituitary_26	Pituitary_15	Pituitary_24	Pituitary_17	Pituitary_23
class	Iso	SP	SP	SP	No	SP	Iso	No	Iso	Iso	No	Iso	Iso	SP	Iso	No	No	SP	Iso	Iso	No	SP	SP	No	SP	SP	SP	Iso	No	No	Iso	No
DEGHDPVHESPVDTA	1885.7614	315.3773	5940.4819	135.5291	1015.4994	302.0235	2999.2699	649.8108	1280.2935	722.2982	1153.7843	2001.9463	211.4300	2082.8823	2624.8567	958.5509	666.9202	858.3076	2244.9717	921.7080	1067.9333	947.9673	561.3494	641.6501	956.4846	1058.5586	310.0060	2063.2849	408.2306	2649.9505	1156.1560	849.2119
DPVHESPVDT	732.6329	1028.5337	4014.5885	645.3079	1365.0321	518.5349	1767.1760	931.1086	1093.1979	725.0139	832.0198	1024.3551	811.7260	2973.6974	3343.4003	1367.4623	562.9294	887.9356	608.4752	375.6075	395.4023	648.7069	1568.6965	693.4634	1155.6097	724.7612	573.5684	689.6006	976.6171	1945.9155	626.5572	1047.4822
DPVHESPVDTA	1249.1137	269.6404	6305.0354	314.6987	788.2289	413.4529	1671.1142	1041.6092	1586.0529	512.8198	728.3193	1046.6516	856.0159	1716.1729	2391.0070	669.6986	1153.6444	979.8788	719.5391	1025.1596	568.6508	1004.2004	988.5371	512.0745	1259.7007	591.2937	322.2202	368.7119	342.4587	1602.4915	306.4919	1318.2449
ELDQLLHY	702.2578	365.8359	4251.8036	1571.6784	818.7707	204.8281	548.8078	1249.4069	1377.3863	449.4381	1092.2277	1050.3928	405.4825	714.6567	681.3714	356.4057	910.9059	227.7265	762.3889	1083.0735	806.1698	941.2577	564.2068	173.3851	2153.8141	422.9277	195.3941	385.4104	326.4447	666.9212	535.5579	1164.0806
FNPYFDPL	1012.0995	1174.4848	4748.8101	649.8372	3131.5785	663.5226	3182.1233	2665.5744	617.9251	1866.7284	1063.6587	3638.0938	1151.2807	2023.1831	2827.5812	1936.8136	757.6929	805.6482	2777.2952	560.2295	3463.3673	2124.9813	1233.8437	633.3731	2823.7585	1153.8689	1207.7125	1833.1350	1781.8536	2423.5413	1930.4398	1636.6772
GRGREPGAYPALDSRQE	1326.0198	741.4886	2609.0841	371.7051	732.1204	282.7307	1629.1672	569.1655	1088.3462	452.7920	1202.4531	650.5798	821.9552	1038.5726	1869.4307	817.9926	1251.9436	673.1762	1175.8321	141.2280	542.9773	810.7972	951.2708	758.2593	499.8622	789.3013	534.0128	1089.2647	344.3161	1478.3604	765.8975	581.8446
LDEGHDPV	1352.2312	445.8310	3832.4791	777.0478	1438.5080	251.3568	1600.4040	843.0791	1442.6768	622.3652	1213.0130	2881.8211	1136.7183	1952.7677	2312.9188	1167.7425	886.9004	312.2281	2308.3925	855.7655	795.0504	904.3929	499.7565	476.4703	164.6228	892.1065	300.4715	1105.0993	355.4608	1349.2122	475.9357	816.5135
LDEGHDPVHESPV	1547.0438	650.3898	7679.6322	771.7960	769.8642	165.1002	3103.0268	865.4422	1929.8288	1084.3221	198.0825	3175.0574	1031.4638	1455.5047	3473.1354	1144.7176	1084.5068	299.9182	3416.9421	1056.1510	664.9774	683.5782	229.2458	415.9412	1493.6574	1065.8175	800.1504	2698.1815	202.4053	2331.9239	1048.6135	1158.4396
LLDEGHDPVHESPVD	1741.1565	1090.1588	3756.1918	884.2069	861.4507	1125.7106	2442.2998	1140.4123	1234.0712	1680.4794	1155.9844	3057.7296	862.6402	1932.7733	2695.8818	1171.8389	590.7727	682.0118	1817.5415	85.2088	775.4033	1532.0972	623.0253	1162.7772	1041.1613	581.7747	387.9955	1413.6271	455.1361	2254.0117	1409.1514	871.6563
LLDEGHDPVHESPVDT	1292.9461	727.8395	2871.5348	357.5666	763.6545	332.3108	2184.3169	1213.9746	1131.3937	834.4296	998.6953	2261.1455	127.0548	2493.7577	3070.0928	1385.4209	372.4030	1173.5596	1614.5206	508.1667	1150.9921	1232.7825	821.9707	703.1316	839.3849	520.8692	559.6842	1369.8564	595.1630	2825.5957	1593.6751	1192.8120
LLDEGHDPVHESPVDTA	1821.2518	1012.7140	4351.9482	389.2120	511.1829	441.6620	2658.6586	1128.6901	1375.4614	1111.2044	1383.9009	4918.6401	458.4301	3908.4387	5233.0015	1343.5484	826.4898	763.4186	2724.4591	320.7812	795.1379	1350.6451	547.7643	721.2797	984.9467	579.1084	530.2680	1587.3153	461.2161	2103.8119	1792.7832	866.6866
NPYFDPLQWKNSDFE	2315.831	1580.156	9217.402	1017.251	7296.730	3488.784	3609.369	3012.808	2046.736	8959.622	1404.903	4541.498	1877.622	14213.809	9541.547	9055.450	2924.962	8085.036	2842.615	5546.970	6866.857	4111.418	6228.018	5558.072	5908.644	4506.369	4051.180	3436.675	9120.101	7817.956	4476.410	4560.475
PVHESPVDTA	2300.77852	803.91908	2357.86381	309.89067	1539.94873	31.41495	1980.50268	1310.97757	1419.71810	962.12209	1255.38811	972.76220	992.69669	2217.74210	2617.66281	1061.10697	245.40621	925.20858	1975.95257	929.34994	929.95835	609.41952	795.22325	425.24704	116.20671	482.77038	143.83733	659.03649	369.24489	981.55417	617.42924	765.19008
Q(-17.03)KIAEKFSQ	648.762036	490.257675	2219.187855	1054.502744	794.742872	182.790861	1228.999414	1560.506553	1352.194640	377.652837	986.705687	1140.809235	582.222231	787.066333	1302.958552	820.774578	157.366233	351.245859	1311.887506	985.341772	594.580624	242.182839	198.696404	7.183386	466.777579	398.990761	185.164175	774.011911	201.941174	1120.168465	684.305249	793.271053
Q(-17.03)KIAEKFSQR(-.98)	1247.0467	874.1553	3823.9206	300.7102	1204.4845	173.7344	2323.7227	1619.5460	1351.1200	428.5257	900.4637	1145.8669	655.4747	1026.6448	1639.7122	583.8738	564.9869	297.1052	2100.7020	871.2698	1161.8278	1201.7380	716.0285	636.0017	530.8866	761.3176	374.0788	819.7205	217.3973	1286.5438	620.6442	304.5036
SEES(+79.97)QEKEY	1304.5048	708.6199	3776.0365	299.3200	2126.9291	445.1178	2065.6320	697.6933	1223.9372	1134.8902	806.8581	2186.6172	1045.8631	5564.9956	3006.3769	2835.2180	1741.5856	454.4371	2608.2801	211.5961	2598.1653	861.8914	1810.3212	1408.7141	144.6790	1436.5310	777.1765	1008.5372	299.7598	1386.0167	538.6565	593.7822
PESAFSE	1211.6938	695.0325	911.1187	973.4968	905.0314	322.1197	2035.2421	1405.9204	843.8562	1187.4995	2353.4191	4611.2728	789.0011	2653.9777	1681.2529	906.6757	843.9462	204.8347	2512.9213	453.3592	1107.8375	1081.1426	427.1291	454.6414	690.1588	618.7797	445.3988	820.9552	591.2599	968.1920	859.1903	1109.2531
AGTQESVDSAKP	560.4401	346.0885	678.1660	129.7240	476.1345	327.7289	361.6175	787.3637	938.1177	433.4915	1100.8896	2698.2095	671.6819	2331.0576	2923.6993	916.6193	1628.8233	419.6820	2185.6845	1198.0760	973.9220	835.0908	833.4672	747.6965	113.7638	1879.2355	583.4607	1507.5451	845.5982	726.5184	1303.1721	1211.9500
AGTQESVDSAKPRVY	786.65469	611.95098	1062.52653	234.17698	547.83079	556.20172	1293.35197	833.93562	16.80634	479.68096	2057.22056	4454.29566	615.69340	2471.48967	2327.05196	1019.29602	1060.08789	468.86056	2244.08867	165.77844	713.69729	1215.42453	649.38641	807.39464	488.83688	1187.05892	408.51697	2062.22508	499.52267	1215.00740	1785.69270	1300.91702
EGFFRLT	1230.1377	1958.8410	1031.6485	2342.0271	1202.3213	172.2985	734.5474	2043.2521	996.7602	121.5749	686.9833	449.8815	986.6367	539.9242	288.3618	143.8977	725.3037	543.0087	548.5690	875.9785	2127.9564	1540.6158	886.7717	309.9098	940.8420	424.0862	439.1154	227.6231	555.2341	543.3309	317.1612	557.2232
ESVDSAKPRVY	1444.9525	1526.0621	1054.1382	1418.0836	1134.9757	403.6071	918.2739	1753.6362	1075.5793	272.7140	1272.5725	729.4929	874.2181	2605.8687	822.5988	198.5225	1145.8385	716.9923	1290.5738	1497.3902	5531.0265	1912.5707	1913.4536	999.7725	1998.8271	953.8088	782.6134	489.1334	658.7417	2032.1677	488.5019	1838.7210
GTQESVDSAKPRVY	1673.2151	1786.4927	1253.6230	1771.3916	1532.8195	386.6178	1598.2296	3519.9155	1134.2284	378.3671	1906.5696	2594.3285	1047.6141	2248.8234	1145.9222	773.2307	1375.4317	413.2697	1653.5683	939.2444	2382.7143	2029.7196	1268.9284	1007.7866	1007.7835	895.6461	548.5253	641.8311	442.4100	1130.5244	754.5976	705.2057
LVQLAGTQESVDSAKPRVY	1331.9636	2794.0616	1436.0362	536.5265	1078.4603	696.1702	3033.9700	968.9630	1058.2805	2018.5515	2233.3309	5646.0535	156.4235	4772.1196	4037.9473	1114.8348	1002.0355	781.4616	3390.6480	180.3138	1873.3325	3046.3006	435.7611	926.3348	2082.0998	916.4442	402.5479	2343.6009	711.3025	3140.6866	2412.4114	994.5508
SVDSAKPRVY	1106.898314	1451.941429	1239.032496	1302.987221	2846.659473	109.074972	626.471318	2075.988969	82.015136	269.742415	767.384549	627.836624	2781.206096	1265.401987	303.368511	874.575888	1088.381466	6.524589	1296.060453	812.416729	2983.849972	1417.925507	2035.820461	720.520771	125.901795	473.919354	370.672050	325.123366	279.514976	414.564210	123.923495	193.166119
TQESVDSAKPRVY	1468.7893	2134.5727	1357.9288	2709.8731	1210.4051	327.3918	731.1437	2049.5472	1051.4123	213.2976	1087.2968	881.2536	3152.1248	995.9067	475.8964	570.2317	1677.3585	149.0985	939.3991	587.0811	3288.8471	1601.6319	1881.9470	629.5968	691.2469	638.5582	564.1372	547.0745	340.7952	618.3729	494.2948	281.4454
VQLAGTQESVDSAKP	1170.8995	1262.7347	1101.3112	528.3992	781.4343	599.8334	714.1240	1251.7847	863.4184	794.5367	1667.5972	3682.0400	853.1556	1468.3244	2184.4545	597.6338	943.4746	509.2556	1914.5201	347.5251	551.7294	1263.1699	711.5009	396.6861	1128.1892	819.5700	479.0980	1441.9008	565.8726	1878.2426	2670.0508	952.9319
VQLAGTQESVDSAKPR	97.70819	1510.03559	925.36166	926.30522	601.63389	475.83771	1686.01293	1104.29894	985.18240	680.49241	1216.58096	3304.83207	855.49834	2216.44518	3145.03069	612.99844	1375.25874	789.89220	3614.29275	312.42326	1088.62772	1094.92037	797.47840	1026.21252	956.30103	1598.53394	411.31508	2336.31883	422.34975	2472.77335	2084.57452	1169.91427
VQLAGTQESVDSAKPRV	784.5790	666.8922	878.6167	102.2969	738.2091	289.8594	850.5117	1250.4947	922.0996	839.8736	2515.6296	3589.5467	864.2456	2069.4518	1692.3907	775.3783	851.0527	606.3750	2078.8774	111.4160	512.4719	1041.2414	836.5499	717.1700	1390.9877	1072.6409	337.3271	2049.1140	429.4559	2383.5806	6652.8020	1191.7903
VQLAGTQESVDSAKPRVY	1134.43246	1379.15881	949.49222	415.87915	380.02975	480.14511	1074.23667	1097.40363	39.77226	789.38857	1737.23553	3274.86751	341.63741	2120.96662	2203.33426	724.83519	879.24004	557.63001	1814.78582	225.75926	871.93911	1261.15881	686.76286	682.76992	1013.51099	824.12285	513.83795	1584.03837	455.63557	1626.32931	3147.14733	1033.28305
AEAFPLEF	1788.8703	1346.4322	316.2812	954.2968	768.2545	315.1813	1306.4329	1452.6660	734.4082	3883.1150	514.9268	2339.0552	3916.9263	3173.0992	641.6193	599.4694	1987.2382	644.5480	2226.9926	5766.3201	5773.6078	3604.3808	855.8742	2983.2154	3106.8385	203.5269	774.9942	664.2808	342.3983	1481.5504	922.2651	2000.0931
AEEETAGGDGRPEPSP	1092.61332	387.92785	1358.01289	259.16276	783.96365	831.53928	1814.73843	456.26035	50.35417	894.39018	1182.53524	3305.74991	964.54242	4476.96183	2761.81928	1240.18000	1227.39591	958.62309	1633.71930	396.39063	1205.51994	692.28755	591.69938	887.11844	642.28669	1249.59876	944.58709	1452.00990	563.60828	1255.26137	537.06119	929.52899
AEEETAGGDGRPEPSPR	1574.16955	415.52800	403.27285	408.11565	929.66828	820.29554	1987.17175	689.25905	96.50598	850.47216	895.65337	1459.58967	1185.71522	4689.09203	2608.30744	1271.36900	1801.54109	1098.57328	1637.89042	193.25777	2295.31062	1267.75877	1131.80189	1460.94836	339.98263	965.14843	1031.43845	1139.12924	544.21438	850.59475	572.65393	558.27768
AEEETAGGDGRPEPSPRE(-.98)	1258.60009	436.80827	1097.59024	310.43784	870.81674	502.04478	2117.71058	531.86646	57.69867	754.15278	947.96980	1268.75600	1233.51278	5315.10102	2957.40687	1322.99808	1394.19186	995.00017	1258.72585	188.36687	1842.36201	731.44521	877.81923	1193.43963	330.77806	961.69672	901.25834	1047.76832	353.84769	699.66807	431.75720	597.81766
EAFPLEF	1639.8957	1585.5946	267.4349	4713.9483	3306.0716	573.9387	365.7458	5288.6401	4007.3421	2968.8946	1614.0220	1308.4198	4310.5163	15.3331	554.0825	1136.3919	1914.1394	1271.9689	1275.6955	6936.2383	3373.2907	2623.6705	827.8183	1330.4972	2466.4561	1141.2130	2214.8758	1016.1028	1579.4345	729.2176	641.7115	635.8778
EEETAGGDGRPEPSPRE(-.98)	1328.22810	374.46431	1740.49302	320.95330	1254.36871	639.88039	2185.70808	856.20888	50.27415	787.76024	1124.23105	1007.90928	1495.00298	5094.30033	3316.87292	1367.75517	1504.54289	1144.35307	928.38044	162.45855	1809.22625	811.78562	1148.85809	1256.66882	226.29333	1592.42097	835.04295	1249.51683	420.71781	802.27284	590.17772	799.19900
EETAGGDGRPEPSPRE(-.98)	964.447425	472.652860	1856.817118	116.773165	887.195075	53.007728	1668.512822	242.570742	7.467272	838.163143	317.895204	464.501467	233.065648	2876.114192	3096.100263	960.799828	843.711851	132.738253	584.050711	26.203379	2645.481483	286.662080	623.952897	985.981055	53.528463	689.728920	732.059206	165.648433	295.614002	310.592386	277.839007	554.548588
ELEGEQPD	2141.11730	536.02797	362.99339	1213.84991	1396.14968	13.78734	2210.19097	2096.72541	162.90587	1355.08064	1156.44958	1505.53182	962.67179	3290.43532	1477.03985	1563.49522	309.18790	988.93979	1721.71896	1063.48716	2992.95519	1654.75888	1099.93792	819.49398	161.90284	543.23006	609.99215	699.75075	546.00888	824.68414	543.06230	391.70203
FMTSEKSQTP	1196.0652	302.8209	376.2465	510.5442	1366.8117	590.7248	3802.7696	1491.1086	674.8852	1191.9260	452.2769	2075.0749	872.9097	1360.7285	1780.6104	666.3682	574.9578	490.2299	511.8672	149.2653	1812.2435	1403.9515	670.4395	928.8478	786.6103	318.1698	1067.5911	875.4247	246.7506	1235.4291	368.1579	381.3907
GPYRVEHF	2525.2346	841.7449	1364.3366	1497.5078	1161.2045	193.5744	1405.6974	1629.2526	990.5812	662.7851	867.3793	773.9364	979.9220	713.3220	463.6081	444.4921	836.0668	219.1525	1093.7493	1300.7445	1363.6070	2386.3122	453.0834	459.3762	1039.1320	411.5144	401.4586	454.0582	265.4576	687.8080	390.8547	195.8287
GPYRVEHFRWGNPPKD	2482.62165	1404.55170	934.04731	988.75643	813.02999	31.79927	1841.60231	138.25794	793.26428	1074.67207	1149.32524	87.26251	753.93905	90.90725	528.73505	704.46806	1385.59784	1068.58032	864.37126	365.48697	74.10039	1576.82324	851.61870	985.36985	727.11815	1829.93951	1880.06588	2179.54540	687.24710	1719.29117	2651.24339	329.39691
KYVMGHF	1451.6570	948.6784	969.8595	921.2255	1108.3420	359.1548	2115.4550	1418.9474	435.1711	528.0728	1145.1101	1190.0108	669.0182	641.4110	672.9088	409.4722	566.5036	203.2024	1267.9413	266.7916	1155.3978	2144.6600	396.4836	489.1483	595.2073	401.7588	456.9299	629.5165	297.8948	1160.6856	579.6359	518.8448
KYVMGHFRWD	1928.28899	795.05945	54.52181	368.64178	14.93975	276.57541	2047.60025	863.16898	59.08809	657.12215	1030.58860	367.39099	230.55954	208.99370	1129.14649	562.98735	414.78856	503.04144	1232.02056	296.50808	464.54342	1146.05922	360.60660	429.95402	698.42020	883.97179	579.75479	1455.18452	435.64658	2101.93453	2267.41833	418.06433
R(+42.01)PVKVYPNVAENESAEAFPLEF	997.4779	487.0765	602.0588	423.0528	130.7358	837.2039	6554.4891	659.2906	748.3384	3557.3633	1540.7474	1072.4529	1547.5307	3289.4445	1924.3251	7284.3099	3334.9107	991.4729	12816.9442	1931.4444	2200.3785	2569.8048	1980.2885	996.0205	2942.6119	1388.2738	4999.0705	10064.7952	1870.9952	7631.1690	4428.4504	4744.7683
RAEEETAGGDGRPEPSPRE(-.98)	692.57913	252.52215	502.07747	187.24536	332.31982	300.85759	3182.64458	962.75671	62.81772	746.45993	645.14877	451.88011	3786.44365	4037.65464	4037.65464	3117.86536	2804.32327	1170.02599	1264.21530	651.12160	1284.60687	1348.73542	1081.98911	2407.62061	210.42662	2115.32309	900.99857	1583.29711	334.14461	574.59093	429.45792	180.77972
RPVKVYPNVAENE	2171.58142	596.48588	1434.44313	2018.03369	1617.29850	164.75777	1624.75986	3261.25976	345.80038	637.12536	584.78013	703.62118	2250.15909	571.37660	383.71038	333.25246	365.01575	223.44422	517.75139	1247.85698	1759.24274	1022.35239	402.21560	258.34617	1029.18928	195.05981	216.54715	212.95797	81.24468	763.86829	214.02502	90.93107
RPVKVYPNVAENES(+79.97)AEAFPLE	2840.0727	856.2190	208.5560	1252.7173	594.7786	1105.5723	4294.8785	2179.5960	139.2318	4246.5707	1879.7018	2600.1837	1300.0918	4271.9063	2374.9409	1869.5284	1792.1812	1868.1211	1426.0755	1039.4052	3908.3056	3077.1275	831.2497	2706.8409	2332.4407	1231.2946	4110.8716	1986.5934	2063.0765	2810.7370	2444.1191	1476.6553
RPVKVYPNVAENES(+79.97)AEAFPLEF	2421.65156	748.26312	86.98168	924.75261	1824.00012	2931.42876	3364.16349	1722.70470	277.34162	8116.00476	2090.04527	5542.79355	3456.42921	757.95228	303.78728	3788.98737	3231.09497	3283.45993	5879.40138	3200.46272	17411.39149	6483.67055	7796.07546	14792.02738	8483.43958	8679.38581	22783.94652	10759.08585	19442.03006	15496.15641	11669.07535	9696.83727
RPVKVYPNVAENESAEAFPLE	2641.9716	1240.7481	274.1326	1425.4898	249.3012	495.1874	4440.0358	1810.1239	831.6797	1839.9635	1673.7284	1573.1440	1021.0049	1915.5234	1803.4833	1190.9838	1113.0322	1300.9318	1115.1564	520.2764	1618.7461	2419.0065	547.7051	767.1221	1853.5394	699.0785	1281.5981	989.2116	838.8879	1682.3850	1648.5382	997.4851
RPVKVYPNVAENESAEAFPLEF	1919.48534	883.63973	80.09351	1056.55269	469.80781	892.20382	2581.96592	1386.55334	116.51693	2924.39366	1264.24477	2017.24038	1382.60296	2450.96514	2236.21390	1628.35941	1085.14702	2063.80766	2876.48529	1006.94549	2548.98781	2992.61696	1610.80989	2204.69434	4015.56385	1834.65876	3922.37390	3012.95253	2501.16286	4789.62727	2498.21664	2473.39992
RRPVKVYPNVAENESAEAFPLEF	1717.4102	1108.8910	749.2999	1200.9545	1267.4521	1054.7058	1825.9609	1670.8577	927.7872	1032.0617	2246.7690	1412.4723	1194.4507	1260.2467	1203.0667	1717.0851	1191.1144	1768.1437	1624.2985	1234.0561	759.0524	2996.9964	1342.6011	1223.2796	2179.1850	1500.6163	775.8288	2077.3838	1235.0924	2172.3807	2291.7981	904.5234
S(+42.01)MEHFRWGKPV(-.98)	2414.13426	1504.70012	79.70621	569.33004	344.42896	129.51668	1088.47909	1352.81428	793.40739	448.15442	1408.97276	597.10736	428.45871	483.39155	1577.86298	510.75333	512.89719	328.20294	938.73701	206.37087	1347.73066	1304.56491	358.89967	508.63926	1159.89186	1135.46111	754.67912	1125.24013	579.48697	1173.29368	1915.03106	241.85134
S(+42.01)YSMEHF	1672.58536	1502.93742	82.01351	1162.26243	452.29551	1018.88074	1148.14621	1771.10306	139.83983	1231.85198	1651.30925	3184.47518	472.77208	1468.34887	1000.14414	421.47607	706.61352	303.51185	644.09442	553.60491	2927.21309	4168.35144	584.28180	856.63626	1051.32769	346.44472	1497.24054	433.29234	1077.98192	1585.22054	943.23091	325.93320
S(+42.01)YSMEHFRWGKPV(-.98)	1332.19172	1406.05582	90.83699	365.90674	333.95789	206.14995	1785.51924	1174.24858	36.30687	998.57360	1303.01738	717.62239	412.94762	795.93473	1694.16828	496.92378	640.11037	640.11037	671.29245	326.56063	1777.56381	1576.52981	716.23443	835.60684	1214.81470	990.67336	1404.53168	975.15367	726.60018	1463.87500	1756.13274	218.02944
SAEAFPLEF	1573.70324	780.53660	53.36996	5639.80461	3141.84022	207.11572	349.42454	5413.03302	5382.04813	261.65689	595.08729	315.99641	3376.39999	921.10576	269.75240	489.34919	386.62823	154.87222	533.35321	5612.33998	3261.93087	2413.82885	941.98808	260.45753	2806.93554	675.72435	438.01999	223.26262	278.28813	199.12396	132.21279	340.30423
SMEHFRWGKPV(-.98)	1302.15233	1957.45626	209.82418	931.14747	776.71301	374.04259	2918.27269	123.10303	800.33600	1376.03061	793.67833	1005.88372	650.31552	763.87319	1912.05526	736.99571	329.25709	833.86383	1387.59898	75.43282	825.70860	1851.58897	279.75836	224.28491	835.60228	389.39199	535.62653	1203.03420	291.06142	2003.54281	1511.44458	422.23786
SYSMEHF	995.7980	760.6201	1612.3022	682.0463	434.4840	386.9128	696.6442	1584.4842	921.4873	1059.1935	1026.4011	3766.9841	421.7065	2385.4177	1269.4927	632.6266	698.8279	651.7055	358.6026	364.5800	3721.7691	5560.2334	1151.4473	1368.7015	2139.9531	267.5529	847.2509	410.4298	339.8800	2111.6886	1579.0946	270.9689
SYSMEHFRWGKPV(-.98)	1313.79144	655.48909	409.33255	188.44273	46.68994	114.92907	1870.57577	546.28697	37.81192	862.81906	670.56357	433.00215	370.10798	562.20221	1141.40406	421.34809	639.20991	760.73271	657.25292	371.45080	1085.45442	1332.61456	634.74826	1070.42672	1140.65342	332.35680	1039.50015	651.36061	233.72671	2772.76599	2896.24950	292.99268
TPLVTLF	1143.77644	863.09511	33.05753	5427.35486	2203.48835	230.22654	454.31875	6066.31893	3811.87309	854.96642	287.78231	334.87941	1955.82534	258.60992	874.97682	466.51352	343.44072	454.92328	155.01942	3966.96812	2669.25028	3620.04891	360.30394	459.97023	372.37512	709.04621	360.59350	462.55442	343.15389	1241.21871	1317.56888	690.06096
TPLVTLFKNAIIKN	2347.03327	697.13859	1011.01342	3444.72716	876.06795	101.21033	386.92989	5057.00364	1222.76611	255.59277	289.69354	555.17280	1966.13430	77.13743	844.87211	243.36315	315.50989	66.83099	395.85395	2838.66112	1569.20558	1187.22791	584.46029	84.30710	2341.81294	155.61156	402.04497	102.15001	178.38536	171.14030	144.53192	76.19674
Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLF	19802.06447	460.46038	77.23413	3509.71024	15004.76037	35699.05812	13620.17264	16049.86922	4818.47998	133732.48766	4546.16359	49334.00316	12920.81035	44435.54340	23181.43409	30047.36098	8511.67570	7476.47190	7114.45767	26814.22042	37054.60032	12977.64804	2977.59892	55044.40509	1469.29554	4367.27254	15206.78160	1957.21353	61882.48662	8193.07200	6335.34056	10908.21795
Y(+42.01)GGFMTSEK	1851.14209	461.16171	885.37677	5191.13414	1104.43253	107.88225	317.58307	4428.38960	1087.42408	215.70215	963.51848	128.20553	1415.15655	232.77972	50.71823	368.43943	206.57147	489.55965	347.54769	2370.47925	1200.37118	1683.75419	263.52704	54.88632	1574.48443	79.88270	273.36909	130.79156	174.12497	78.04398	860.44609	223.70365
Y(+42.01)GGFMTSEKSQ	3048.31112	1634.30462	1096.42727	7793.50682	1504.85867	180.53534	1113.04213	4893.61346	1041.59815	249.67572	461.60871	274.98982	1666.86135	209.85049	799.92512	390.51011	405.96778	10.92142	796.11758	781.21794	2428.99926	2914.79911	580.28512	187.61869	1349.91291	185.85351	495.36233	206.04511	189.79614	190.84358	70.98919	294.34544
Y(+42.01)GGFMTSEKSQTP	2359.5823	530.9060	354.3483	947.5756	928.2998	1586.3351	4262.0317	2820.6308	1102.6626	6139.9133	3007.4685	6370.4620	2390.4061	5109.9465	5330.2759	1686.3671	1657.0338	1296.1508	2010.2464	1107.5718	2596.0385	2330.8086	1083.4499	2327.8773	1285.1935	845.7526	2607.8376	1808.9810	1636.7229	1646.7610	1626.5965	1727.4186
Y(+42.01)GGFMTSEKSQTPL	1890.5524	890.1351	829.4067	494.7415	947.6413	2499.0895	3482.9431	3912.7606	1011.5112	4217.9946	2141.2533	4266.0922	532.7789	2479.3919	2874.9370	1726.8917	1155.3556	1325.8179	1561.0180	1143.0749	2384.0112	3095.3694	519.4710	1241.1252	2846.2408	1305.7566	1252.6419	1680.3733	2381.0004	2116.5915	1964.5711	2701.2853
Y(+42.01)GGFMTSEKSQTPLV	1679.5974	891.0559	272.7393	1155.6886	1172.4591	2767.4797	3216.7469	3347.3083	623.9381	4347.0892	1334.3863	5122.7979	795.2875	3853.4215	2894.2729	1271.5324	1291.6477	1808.9979	1769.0802	1147.1083	4148.3174	3587.6156	561.6730	2103.1066	1991.8828	1514.1839	3252.0562	1533.3860	2851.6095	1293.5299	2442.3292	1741.2903
Y(+42.01)GGFMTSEKSQTPLVT	2238.2991	933.9005	93.9287	635.0525	498.6180	2190.9864	2639.0331	2422.2990	286.9569	3317.9396	1736.6666	4918.6429	791.7815	4125.9539	2729.9545	1472.6843	1075.2771	1778.1790	1365.0640	540.3638	3702.0823	1869.0124	934.2013	1508.5352	3993.5044	1268.0500	2340.0106	1738.6443	2446.4184	4213.1632	2220.6881	2584.9512
Y(+42.01)GGFMTSEKSQTPLVTL	2526.1387	807.8641	297.6504	1022.6535	1939.8358	4615.7701	4981.1672	5190.5885	693.3660	11242.4899	3231.3855	6708.3664	1906.0279	8951.0141	5674.7451	4692.7936	4577.0315	5674.0060	4459.5530	1987.2440	11005.5246	12487.9014	2869.3512	7651.6032	8357.3145	8395.7620	16219.6104	12215.1838	18105.9961	11777.0431	11055.2243	15614.0797
Y(+42.01)GGFMTSEKSQTPLVTLF	20249.9011	2665.5482	135.1795	6224.5429	42098.5507	193727.8441	38131.3940	70039.9302	7508.9752	366238.7303	23909.7801	137130.3061	49619.5187	162121.0470	44095.1697	85682.2316	36959.3846	51400.4686	43793.3459	79788.6761	286978.1573	358578.3414	11989.1627	214757.3324	8880.1365	15189.0568	118388.2165	64654.8194	762528.5737	195106.0964	123152.0751	139064.9836
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIK	4449.2819	1023.5218	629.4436	552.6407	512.5445	5225.9444	4654.1233	5392.5907	979.8480	16967.2216	8237.9843	9857.3180	5135.3447	10183.7506	7031.6373	8815.0104	10294.5153	23976.1356	12396.9544	5945.0935	17092.0204	3377.0524	3730.6363	7181.7787	26491.1635	8826.9952	15488.5946	5894.6884	19999.8357	16499.7054	18287.1735	14987.2324
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKN	3606.5361	1356.6643	425.1727	668.2726	899.1507	5438.9801	4614.8109	4367.6985	380.6630	14995.8154	5114.8226	8364.1552	4395.2816	7700.9479	6871.8985	10325.3165	6161.8616	7107.1177	10057.1391	2904.3825	21908.5085	11578.5900	5471.4165	13198.2261	21143.5987	9156.8055	17514.6952	5991.8694	26057.9782	23909.5540	24741.6731	22023.4175
Y(+42.01)SMEHFRWGKPV(-.98)	2570.54781	922.76075	58.47335	498.77771	267.74553	329.35579	2864.62580	1363.56467	880.99319	1040.05356	1044.15139	984.05002	501.76300	634.88220	1619.19569	595.50966	287.07529	503.98760	921.21387	499.81385	824.58232	1080.66378	421.15262	305.25804	832.97727	529.22379	743.13020	1486.26041	377.26292	1637.43361	1631.92036	501.15392
YGGFMTSEKSQTPLVT	862.44418	306.24218	1468.56263	78.50381	148.58608	230.08968	610.34886	2030.29961	707.33272	1040.79992	957.70020	1261.13581	447.43594	3572.92826	1491.18080	588.60416	1269.90535	625.10236	2564.46286	1707.50218	1728.30664	4416.07762	1111.62286	1041.38295	4545.97750	363.51430	1440.54830	2016.00928	259.60368	1794.44360	1258.55771	491.93625
YGGFMTSEKSQTPLVTL	2217.2064	972.8804	7864.5741	424.6870	1251.2668	1062.5511	1905.4961	2973.3009	2302.5330	2870.4386	2424.7719	8677.0149	2016.0880	7143.2969	7378.2738	1667.8136	665.8644	767.1069	3797.8193	911.7435	2829.9190	2278.7429	689.7764	1305.2056	1822.0938	504.1923	509.1627	583.0309	1622.7909	4254.7428	4175.9513	1422.1872
YGGFMTSEKSQTPLVTLF	1166.7785	1300.6537	494.9394	879.9662	831.8420	2236.7307	2839.1868	1490.2617	985.2785	19522.0565	1729.8214	7920.7987	1632.6994	8964.1245	2136.9881	4173.7291	1591.3818	4065.4831	4179.4488	1602.4214	11154.7698	13816.7036	3001.8755	19980.4832	5543.2279	9098.3142	10916.5076	7342.2473	9829.0264	14099.4422	16113.6483	6033.5451
YGGFMTSEKSQTPLVTLFKNAIIKN	801.6936	997.8001	761.2405	998.4241	1014.7552	605.0263	466.1262	1622.8692	778.9066	6082.6686	1484.1961	1722.1758	676.3977	2355.1457	1062.7664	1273.3868	1039.6679	4275.4256	3246.0436	1417.9440	2867.5134	757.7882	1415.2987	3742.6789	12978.6182	804.7154	2406.3565	274.0186	606.4966	7816.9887	8148.2930	1387.8961
LPGLLLF	2617.5551	4001.9509	401.9000	1510.0287	10970.5040	516.6418	372.6661	9559.8024	11805.2880	914.7902	798.9089	398.5133	6130.9744	2384.3979	538.8739	1241.4765	876.6829	1125.4433	386.1405	13069.4573	14767.3217	2654.6208	5215.6111	2870.2262	627.4914	1425.8802	4726.3510	2034.6941	3409.5923	2278.1160	2239.9032	1996.2605
LPHLPGLLLF	2610.7415	3267.2142	816.1083	1103.8026	2848.2715	300.8810	896.2225	3657.7686	3836.6507	2271.6777	682.4394	255.2394	3666.8194	280.8042	1208.5243	1949.3068	1021.2254	728.9690	1260.0581	16438.0731	12871.4877	4932.3422	8699.6568	9843.1286	1264.3956	5636.6774	9467.5861	374.9963	5923.4349	7456.6194	1672.1938	3719.7781
TPLPELFDRVVML	121.40625	79.41163	3408.89619	27361.48209	1788.80935	79.42314	426.26655	4397.04419	48376.03196	137.44936	125.60843	254.53592	14826.19877	794.84589	1071.83614	176.46980	2237.50357	1526.62325	271.71916	13869.96078	2644.13063	6410.27723	2004.16002	2969.75404	16868.09413	9028.70706	1159.10809	4056.29251	3323.22917	6017.36341	8439.14554	1441.32837
F(+42.01)PAMPLSSLFANAVLRAQHLH	3421.3759	6605.7287	609.2966	4294.3793	3059.9351	3454.5207	4992.8880	5341.2528	2346.5428	6954.2496	1997.0283	2485.7921	10321.9558	3398.4541	377.2753	12404.2042	1798.8780	4063.1344	14962.2605	4950.1811	1976.9799	8989.6634	5739.6362	3201.3152	1865.8553	735.2744	13304.9650	5655.9568	8349.2695	16537.7369	2465.1564	3746.8310
FPAM(+15.99)PLSSLFANAVLR	3255.2301	3768.2826	796.7819	2086.8098	4642.3729	2551.5332	247.8049	3146.5518	1243.4994	5623.7333	1136.3719	2327.3740	9856.1390	2264.8202	887.6879	3616.0185	8155.8752	1190.7523	2359.7055	8776.8050	29673.0081	5150.4962	17466.5812	13560.5525	2936.0263	956.9824	14290.8732	5764.3957	15243.3295	2087.1987	3915.5896	1210.3601
FPAM(+15.99)PLSSLFANAVLRAQH	1666.5542	5319.6743	270.2873	2324.5532	979.7854	2551.6114	1429.3293	2260.7374	1209.9685	1885.2049	847.5780	847.5340	4736.5349	724.5100	421.0874	642.7984	5659.4351	360.5270	446.9430	4099.1815	8647.6293	3055.8494	6219.5315	5210.9589	593.7642	465.4796	5051.3563	1746.6904	2042.0043	1191.6695	376.1937	2351.3405
FPAM(+15.99)PLSSLFANAVLRAQHLH	1490.8871	3990.7585	642.1378	655.7635	1823.6623	2055.7784	209.1228	1140.9750	892.2782	2119.6422	1390.9029	937.0781	3563.8098	194.1980	379.1971	300.1102	3491.5606	773.5793	1003.4034	2232.3530	4281.8377	4744.1623	9700.0173	7186.0961	1697.1705	951.3213	6128.2791	426.2509	4194.9080	2002.1764	435.9952	350.1876
FPAMPLS	1068.34641	2456.14935	455.59266	2901.08328	4733.37971	336.30588	250.41095	3936.01730	1238.14279	213.12937	279.06187	503.40171	1901.19674	393.91739	170.40789	221.57853	262.55347	46.82605	537.06079	1467.46050	1965.64537	1181.30026	1231.29443	265.43623	701.17196	159.35975	636.73512	146.97570	239.63729	428.44533	172.15624	281.09126
FPAMPLSS	1204.8064	4512.9190	1467.8111	2456.2162	1971.9384	686.6081	536.8247	2574.2500	935.6858	212.8409	816.3777	1751.5270	1372.8184	852.4849	570.5091	201.2290	578.0527	141.0617	785.1445	947.3571	3628.0505	2440.6885	1733.2408	616.0395	579.7279	403.1906	1541.0080	369.9720	723.0883	1186.4724	590.5848	383.1179
FPAMPLSSL	1286.8753	3741.3131	1912.9228	2628.1146	3700.3144	1416.3883	397.5944	3869.8595	252.0009	761.9295	1089.7972	2281.6608	2119.3669	1033.7339	586.7397	595.1666	812.7680	262.6326	1082.5006	938.9633	4687.7794	2667.1848	2223.8858	1036.0567	1304.9731	883.4996	1931.5178	586.6091	1096.3368	1329.4168	1053.0381	671.7053
FPAMPLSSLF	1475.8811	3700.3976	571.2236	4350.7708	7425.9673	766.4074	183.6427	6774.5138	2296.7471	601.5102	903.1957	1201.5230	7045.2939	1211.5349	341.0623	413.7503	1550.4410	131.1912	640.6707	3313.6992	9217.0998	2727.7336	3850.6723	1746.3612	1070.8167	710.4724	3081.9846	104.6178	3455.3921	1139.0920	390.3502	610.6355
FPAMPLSSLFA	1839.5765	7621.1026	2953.3337	5493.7012	14966.9471	4108.8506	333.2089	7705.1075	1004.1974	3197.0367	3689.3573	6074.7502	16506.5626	6979.1583	1701.4549	1067.8184	7987.0361	806.7986	2227.5160	6232.2826	64671.8328	16868.5697	34593.9989	23367.7276	6447.0772	10152.4982	52947.2060	1497.1722	63981.3506	19024.9772	6643.1478	6042.7694
FPAMPLSSLFAN	1972.0170	6183.6818	2488.1764	5281.3104	12561.4821	3732.9602	476.9594	8106.5447	955.8985	2532.2926	2498.7523	5104.4835	11858.9011	7461.0492	1609.6429	1120.1124	7951.5861	854.7210	3012.6771	6001.4959	53646.2757	16250.1340	33508.5099	15148.0619	5195.9248	6056.8617	37456.9080	1269.8157	32799.1241	16383.8030	5362.5701	5494.1669
FPAMPLSSLFANA	1846.01250	5559.25603	1932.00168	4362.92687	9503.90153	42.37256	3350.72865	7267.55441	753.62693	8254.95926	2561.67665	6205.36247	14491.03270	422.95160	2389.77135	2076.03577	8536.59493	4305.23324	4028.92985	7068.88407	80335.15646	1661.16764	55610.44759	31384.93824	11463.12516	13658.46446	68060.82292	1350.41315	81073.24443	28432.60781	10865.31799	17855.45205
FPAMPLSSLFANAVLR	2054.9066	6900.3285	385.5306	5487.0119	4787.3505	2529.9699	335.0111	6051.6238	1349.7004	2629.5886	2828.3359	3624.5485	20894.4559	2511.7389	1026.3796	1245.6723	8655.7486	963.7901	3722.8375	9360.6518	73143.8633	31305.5735	64060.5766	43528.3405	9601.5044	16579.3581	103620.9881	2502.2550	116169.6393	30758.6542	11947.5764	7151.5989
FPAMPLSSLFANAVLRA	3843.25391	6126.92652	34.04890	6855.48239	4507.94879	816.36486	141.85169	12051.06340	1147.28737	588.46947	1043.21912	805.49705	8462.24429	257.95054	78.09512	336.04565	799.60764	57.67205	1544.69532	6284.34066	8801.13195	4151.47733	1266.40677	4601.96997	2127.99391	1392.69261	11732.88971	273.76743	16043.03417	2991.06027	840.93707	969.94446
FPAMPLSSLFANAVLRAQ	7348.76218	5184.63852	27.25108	13396.53853	10485.70871	4408.00494	742.14799	16737.47440	2013.99573	6626.69563	10947.43017	28493.88473	85032.93159	12786.64545	7390.74648	6386.90166	38875.99606	6745.15145	16978.49642	33203.21482	65090.75163	32348.13111	26365.04984	62096.63053	5220.97368	15596.62830	88354.30099	1832.90299	114082.75433	20086.06777	10061.80591	3078.61226
FPAMPLSSLFANAVLRAQH	1572.94797	6056.50325	96.07448	3938.61899	1568.32075	1298.07339	245.47837	2747.84664	129.05421	1049.45421	1498.52597	1009.60916	4893.86902	443.31375	345.25089	483.86252	3400.20194	615.45176	1860.83659	3306.39590	10392.65108	9579.43303	14209.23583	6751.19022	2529.69499	4196.47736	15294.28783	638.92959	13007.29310	8716.19477	3241.39669	1490.25189
FPAMPLSSLFANAVLRAQHLH	1677.80007	6386.09024	78.66319	3425.18705	263.93614	1659.62095	314.23344	2226.00063	293.20284	1030.07273	2107.24388	1268.09112	3871.23527	128.69679	465.75982	714.94906	5051.56727	942.16527	1863.48097	3409.47487	4991.08850	12767.90081	13159.46006	7589.37826	2973.93421	3621.04932	15259.96082	759.05999	14292.58317	8057.85720	3360.34737	1360.41745
FPAMPLSSLFANAVLRAQHLHQ	1851.47698	6709.00366	79.76641	3465.17714	390.25169	1747.15192	505.47521	2863.13081	713.38784	1510.08573	2189.93552	1533.08955	5729.37465	314.27914	673.13688	1153.94138	4608.16004	1236.52295	2488.14174	3170.72341	6675.37833	14216.50538	20881.90687	10893.67771	4936.68126	4423.28160	19904.76720	1080.73994	15706.83970	10577.65792	4460.16452	1701.19936
FPAMPLSSLFANAVLRAQHLHQL	3097.72081	9842.51193	55.57607	6295.07303	154.13812	3108.16978	554.26735	5644.98798	1100.39431	2951.18408	4737.72967	3078.00238	7802.83406	50.19664	425.75161	2199.12185	10178.47818	1715.76590	5106.23740	11954.02425	6116.39016	27927.66828	13469.29544	18393.55398	5027.28715	8063.29424	42219.56212	1975.20980	63028.19632	20626.68113	8693.89039	5966.39537
FPAMPLSSLFANAVLRAQHLHQLA	3894.36232	11711.30697	51.77181	7356.31622	264.35152	3203.82551	473.26927	5831.16087	1440.07922	3102.71614	5809.42087	3245.16949	9719.36334	94.50447	378.39067	2081.64922	9637.53751	1762.29257	4563.53685	12090.97952	7513.05960	31379.84732	17665.46595	23460.30070	7949.49144	8665.41716	45006.43021	2206.65856	53435.91166	23251.65162	9731.24679	5812.91291
FPAMPLSSLFANAVLRAQHLHQLAAD	3851.11012	7418.12196	26.70494	13588.79831	551.32902	1810.86929	475.85175	13179.88801	2061.41155	1725.36953	2106.53172	1639.70064	14719.17320	90.32049	157.22455	1772.13236	5995.57107	457.61650	4760.48970	16973.10640	9298.81520	27726.69036	17349.21298	23634.78941	7902.64721	4199.70303	36631.50932	2507.66037	40721.06928	15830.27010	5343.07142	3268.36815
GPVQFLSRIF	1270.05880	1661.25835	1907.34200	4627.03294	2804.10501	337.05932	503.19917	5745.84195	11837.60915	476.17773	159.38617	577.28363	3245.33790	743.55653	1071.75082	78.12899	296.42374	502.34763	246.53783	8204.67634	1212.70099	1127.43295	1885.61832	560.89966	959.21531	1031.93263	247.04607	534.90102	469.11858	523.05529	686.93031	200.37761
PVQFLSRIF	803.84186	797.84304	1488.46827	2745.17503	3956.88718	212.22355	378.41690	6840.81447	9764.48198	95.16232	81.80063	348.34423	2701.92300	398.34421	889.16052	268.93656	238.69654	386.44922	149.63380	5134.74550	823.13484	806.18794	961.86490	126.43869	567.04673	631.84604	264.55974	47.27004	75.71514	390.66403	572.40100	623.43618
SPRIGQILK	1333.28089	1533.70874	1191.90490	3644.61286	5379.92761	327.47783	55.99582	4263.80291	1655.21946	49.54831	45.28199	401.91185	2559.99428	579.96172	919.42807	321.82450	453.06657	310.69172	290.15405	1171.77599	1061.77882	548.94687	875.91694	211.79959	528.62926	91.23637	245.21666	49.04333	69.22670	87.89908	11.80359	428.33760
SPRIGQILKQ	810.02758	521.39706	14.06009	3218.90158	4366.04360	19.58892	31.97756	4776.55666	4473.17821	46.74610	23.24002	139.00431	1760.90366	11.13479	13.02338	56.24203	101.95233	156.81812	98.34171	1845.44760	364.51497	299.18114	374.22671	45.94389	366.26472	32.29326	41.94627	11.18567	19.10695	27.05409	316.95087	62.69558
TDMELLRF	325.343566	163.642958	1480.322770	2063.406140	3609.534561	48.778162	126.240561	3057.285131	6082.149249	49.751678	738.732395	133.194100	1234.476642	708.977888	1029.204997	103.521999	270.691469	297.816314	140.915796	832.492055	241.725491	121.666711	160.904318	14.902548	300.230227	907.135839	38.221790	10.639055	433.544178	148.463269	9.278954	29.609912
ASLDKFLASVSTVLTSKYR	1786.9180	12163.9599	584.0004	2140.1570	2473.6909	1889.4524	3221.6436	1865.2009	1062.0330	2973.7274	5117.9128	2446.9535	1333.5249	3506.4079	1809.8464	3679.6227	9881.6847	1649.3172	768.2713	4372.4544	7162.9681	24894.2498	5272.9167	4017.4604	9108.6328	16618.7726	8860.5191	387.9127	15337.2712	2587.2283	7557.9660	207.7931
FLASVSTVLTSKYR	913.4171	7313.5086	1214.0830	1946.5729	556.8146	801.8131	2558.2876	2318.1079	1046.5319	1802.6585	3521.0011	2266.6590	749.1031	1263.7660	273.9064	1274.1134	2978.8483	304.1508	272.6335	840.2629	4235.2849	5974.7769	1691.1817	621.1636	1886.2092	1199.4390	1348.6978	1145.3901	1316.0498	839.2400	3381.2860	315.0191
SLDKFLA	468.697071	296.403444	55.314391	2295.791994	3716.926388	25.917030	8.224895	3162.446880	4014.250853	16.126448	4.802543	17.793792	1054.153364	31.975905	31.936331	41.552847	105.127265	185.004964	74.238867	3238.709866	306.804755	177.337500	119.042766	7.345192	732.339845	24.755719	5.576748	4.769587	9.546823	155.916133	24.427866	34.466715
SLDKFLASVSTVLTSKYR	1425.26913	7373.78821	13.83642	2221.62272	98.81217	1653.39181	2026.85164	1394.05723	732.10405	62.60866	4591.08131	820.49871	2486.35973	30.42651	1488.54046	130.51585	10796.32769	537.76385	736.97589	4249.84519	18598.73608	27715.76357	9649.33533	3536.86664	6260.02782	6152.20270	8221.46313	738.18243	10618.03977	3176.32401	5953.20702	768.15575
SVSTVLTSKYR	812.28167	3570.76844	407.54307	1522.58819	808.82778	333.47201	76.66810	1409.07874	944.44464	23.89189	1553.86844	862.53433	2894.67302	984.64332	93.95138	58.26344	2385.43439	64.79215	500.45590	946.20178	6361.74657	3371.52500	2030.52258	444.64684	637.38233	675.68587	510.57705	205.29137	393.59597	389.68613	471.11309	630.11375
TPAMHASLDKFLASVSTVLTSKYR	1256.5096	6597.5043	469.4162	282.4691	1843.0427	2096.5146	2518.3276	3648.5225	595.9504	282.1067	7892.4774	949.1916	1503.3780	5335.1996	2644.6272	4665.3396	19611.3574	1015.1761	825.7665	313.4042	4177.7012	20722.0637	7291.0028	2762.7342	4233.8820	7895.5997	5213.8931	141.9581	11216.4423	1120.2600	3526.1876	724.0338
VHLTDAEKAAVN	1345.42034	2252.11666	1293.85744	1575.28971	1769.73769	466.44249	192.41864	2604.74617	1184.23032	76.91805	1816.95551	1773.27056	1451.14767	1087.44745	184.89833	140.65059	2188.13998	205.96149	911.88738	1681.09783	3392.31768	1795.44619	1673.27504	475.15902	804.01064	1098.42313	499.32304	362.34061	408.03963	772.67400	414.01157	283.38760
VHLTDAEKAAVNG	1366.0872	1443.4128	778.7936	1600.4869	1395.1824	189.4421	180.4173	2665.4365	856.0255	570.1055	1114.2584	944.0933	520.7440	459.8676	129.9189	106.2973	1647.4348	142.9464	660.4107	880.0270	2011.8550	2028.4819	997.5653	394.3079	555.1171	756.5446	298.9375	232.7442	255.7696	457.8061	242.8736	175.8147
AAFQKVVAGVASALAHKYH	1345.39639	5271.50768	951.17780	1736.02473	1879.32515	381.94601	25.42779	1921.69401	1001.55261	563.94193	1196.51607	188.66902	1732.22429	879.59833	943.52845	54.67614	1841.75356	87.28369	401.63251	1504.50058	1135.81986	3516.71681	1841.68471	387.72310	1273.00253	1429.96560	1031.09359	372.72640	829.68721	943.74914	1489.76297	277.36805
AFQKVVAGVASALAHKYH	1072.01287	4811.87595	1046.83448	294.11728	298.39487	382.87195	885.53432	1713.01635	1029.70995	655.22344	1226.24139	326.52633	1853.64291	14.65113	950.47143	694.70669	1930.27343	129.53019	327.58437	810.39826	1189.06670	2730.34834	1652.18584	401.44020	972.16534	1102.79711	642.25123	203.93132	531.46489	500.43086	710.02308	73.59277
AQAAFQKVVAGVASALAHKYH	1394.79626	1365.80159	844.93715	1120.06398	919.16746	480.92340	678.11152	614.85706	874.20482	47.39941	807.54847	272.44438	1599.36984	907.29057	1077.96206	696.63799	1818.10955	651.30104	87.51857	382.14340	772.86101	2612.00821	1257.74092	322.36217	1001.16243	1049.56760	444.12684	274.01788	405.09457	498.16353	712.06426	41.09146
ASALAHKYH	556.17282	984.81556	499.36596	1836.86375	1191.86397	203.75198	713.91839	1648.94633	1131.01618	627.77076	973.90105	603.50621	2594.17019	377.56383	956.27145	142.84726	1722.85763	34.45715	351.19204	718.21923	1611.49494	1272.71475	1213.15799	344.18763	392.48560	757.02717	690.52620	508.05554	257.44426	380.96544	217.79086	148.00339
GVASALAHKYH	1046.27394	1910.91624	625.68621	1719.27690	1186.73470	264.11539	115.24825	1912.41565	61.89201	67.14016	1196.92265	415.08324	2550.06298	494.22268	150.30293	124.22978	1851.42614	133.18957	445.78755	1230.45925	2246.11107	1522.36417	1518.26236	456.93858	601.23420	1146.61691	372.53684	262.54977	368.13577	469.55006	303.63514	126.87611
QAAFQKVVAGVASALAHKYH	1505.43318	5878.35813	1188.10679	1915.71450	475.07034	638.37577	1118.33862	1814.49161	1018.49093	627.49447	1182.01187	326.76981	2282.20187	91.50535	956.34754	88.37185	2582.24317	154.42325	165.45291	762.88127	971.54052	2405.71938	1499.19333	486.75108	911.76706	1045.04574	531.39249	90.27270	520.94797	520.58095	625.61044	116.67406
VHLTDAEKA	774.13534	2927.56765	411.72682	752.87189	1326.64242	25.33716	476.28259	666.97385	871.09902	46.98186	704.02985	713.08828	187.06991	1247.01268	113.72011	126.19024	155.11006	395.13726	280.37531	218.52956	4303.04004	479.87820	647.04116	190.68971	91.25183	871.04280	420.42864	136.38905	466.25627	299.40990	147.18890	150.96297
GPNLVARSKEALA	1144.10839	2577.05726	1011.27257	3558.18126	836.11273	228.96083	179.07913	2843.22351	1002.69628	67.57314	1036.07259	282.27333	1539.38884	275.69479	128.80311	64.87881	2135.75471	58.18972	942.48199	714.90559	2158.18599	2830.13653	1337.79885	482.64414	1243.78660	970.06988	446.03534	264.22746	457.91930	348.69890	583.44391	173.43782
AKVAVLGA	3120.02585	2028.01680	423.50199	6615.65245	9009.34349	197.74939	300.60587	6053.74806	957.33918	174.91235	601.21800	760.62751	3473.59169	478.47251	88.98529	397.25578	1398.23545	588.06151	411.83306	1472.74651	862.16125	1483.06673	1331.82109	508.29412	433.89512	259.67334	189.55391	642.78531	238.94778	171.28003	53.40606	293.79283
SNRVVDLMAYMASKE	1297.47645	2845.50008	978.63506	7229.27831	779.48424	557.96415	30.85284	2649.39569	797.36537	122.81796	1418.17526	588.44301	2037.19996	988.02555	999.66219	288.98050	2168.94547	840.68074	1045.87748	868.77741	4678.12766	3861.13407	2303.34351	568.92585	1025.60777	244.86089	924.29156	373.88010	553.33583	868.35216	343.24486	878.00052
VKVGVNGF	927.9235	1271.0619	1549.0529	3558.7906	1610.5104	473.2423	176.9835	2683.1348	820.7649	248.6979	906.8309	1482.5523	803.3213	734.4901	355.1159	321.4603	1056.8016	834.4021	578.2669	687.9629	1541.5131	1964.7975	1141.8634	396.5827	1649.7245	297.0105	366.7290	225.9843	331.1015	908.1633	158.6176	1063.6708
VKVGVNGFGRIG	1225.55561	2786.09643	569.30445	2407.78578	738.46810	155.89882	98.83911	2076.54935	889.32042	575.34020	1004.19218	483.60753	965.88280	491.00713	937.03389	133.71832	841.80701	607.86415	626.17326	818.78197	2045.08059	1987.47268	1177.82641	448.86677	1122.84602	253.79506	544.71259	325.11297	205.78439	1022.25509	304.00397	505.78242
PHFLYPK	1893.3365	754.6866	146.1770	741.2377	226.9641	339.0928	1946.3208	1393.3732	285.8685	443.4026	1330.0927	1110.2141	569.5254	698.5328	1393.3890	636.1447	782.6068	284.2704	1711.3915	314.6141	964.7403	1722.7505	483.0935	449.3893	1338.9285	888.2085	416.1447	1187.6830	320.5185	1313.7763	1276.2398	493.7489
SQENPNTYSEDLDV	3023.4467	1419.1393	1127.0380	1727.4749	3333.8159	2650.6924	2451.5527	1725.3994	934.0012	2925.7623	5201.2693	18238.2888	2641.3207	14280.8395	9825.2176	3513.0865	1463.5611	1773.1606	5302.8428	566.1948	4372.5570	2971.9088	649.6257	4785.9627	5994.5402	1121.2075	5478.8954	4380.1467	1928.3069	6248.7459	2458.0375	4575.5035
YGGFLRKYP	1149.7152	1539.1348	94.8125	387.5346	435.0137	397.4971	1976.0868	676.4533	867.3583	623.3477	1180.2646	1756.0966	219.9302	969.4790	1905.5276	498.6858	518.9525	361.7811	2467.9811	310.9131	846.7218	1278.0706	596.3536	438.9287	622.5176	521.6038	550.4176	1049.1182	388.7402	1613.2434	1178.6253	458.9272
YGGFLRKYPK	1278.0204	1032.0067	1201.7402	429.2760	436.0873	167.4739	2343.5828	978.1891	953.3156	236.1624	803.7992	561.4016	615.1331	558.5490	1848.8556	346.1620	938.8720	291.9937	2066.7880	169.0148	804.6692	1059.4811	784.5632	628.9084	234.5429	631.1069	499.5146	976.3925	286.6674	1391.0829	763.8200	214.5899
YGGFLRRI	1216.6279	1423.7135	951.8118	420.5699	340.5957	352.3116	1685.7508	705.0449	205.0971	868.0460	927.6019	1178.3795	419.6388	413.1621	1298.5093	604.5657	550.8467	527.8237	1996.0159	375.2349	626.8084	1205.4008	669.6316	568.7282	512.4135	621.4927	615.9726	1037.7396	462.6864	1549.1504	1668.9301	834.3053
YGGFLRRQFKVVT	1638.1736	2539.6537	1314.2441	149.2022	952.8939	474.9192	1898.3482	758.7078	840.3499	623.5917	801.3029	881.8229	325.9479	178.7835	1204.9876	541.2856	586.0490	471.7487	2027.8143	454.6111	724.8741	2053.8098	669.9719	599.3727	920.6274	600.7616	659.2353	1279.8097	505.2111	1644.4202	1437.1965	498.6738
MDQLAKELT	1582.35010	807.38765	1131.09039	1354.12230	1289.60881	79.28444	1249.88565	2234.97042	1164.64529	565.99584	418.36656	819.53508	683.34181	2031.86904	3036.95729	963.19453	275.38976	482.43535	739.49276	888.25674	1534.10409	981.14936	870.65523	615.74857	294.70211	224.66691	424.10505	486.82481	199.10695	1710.10612	303.35796	449.08874
MDQLAKELTAE	952.3925	1370.8076	1700.3077	1113.9731	1110.0263	525.6043	1906.5338	1349.4814	1137.2977	930.1319	732.6630	1157.8050	351.9164	2224.9478	2797.6722	1154.2423	1170.5169	461.4009	5228.9187	995.0905	1638.9800	1012.3564	815.5997	293.3359	866.8870	801.8041	588.5666	504.4856	317.1163	2437.5926	1146.2034	1425.1129
WSRMDQLAKELT	881.2570	1085.0233	776.5337	961.4227	1622.7023	166.2280	2677.0329	1184.7681	991.0359	1299.9699	384.0406	846.0860	818.3959	2310.2613	4005.9931	1201.2027	632.2327	1050.9041	459.7984	592.8583	1475.4890	806.4350	1064.4150	893.5829	751.9164	380.3741	690.0624	342.8748	486.9687	2177.4126	760.3663	363.7662
WSRMDQLAKELTAE	1730.9948	1118.3519	1835.4876	1145.8762	1165.7112	612.7908	4856.8393	1357.4575	1247.3719	2466.7013	1037.2030	1113.4530	1310.3787	3272.1776	6334.8559	1938.1825	228.3252	1694.4612	1866.9656	963.5517	2512.4439	1180.9427	1409.7068	1381.4184	1508.0508	1446.5260	1309.5499	1129.8078	945.7860	4192.1158	3399.5980	913.7773
NQEQAEQGREHL	1456.98449	1001.48831	1303.35183	1002.08526	2290.97643	84.44304	2768.35006	479.54307	1147.62993	810.87317	797.06831	670.80503	475.20826	6761.47258	5487.95250	2166.29704	309.77102	937.95208	505.72093	346.80123	3818.66767	158.28101	1437.17522	2141.48625	496.04160	458.66474	602.15453	314.82839	158.88735	905.67110	188.26549	501.93433
Q(-17.03)ELGKLTGPSNQ	1552.0647	1648.2301	1551.2115	1039.3340	1289.8616	197.4548	1627.9439	1092.9097	995.1676	1106.6425	332.4412	1046.5612	660.3100	1826.2799	3644.0852	862.2923	565.9280	603.0708	826.2617	869.4804	1912.4702	1083.7870	468.8812	738.6140	690.3552	523.3472	1182.2708	615.6526	341.4543	1362.4885	688.4342	505.0914
YEENSRENPF	1548.7199	684.6272	3862.4733	343.6947	1067.4420	157.1328	3441.5669	1501.9484	1359.3364	1475.1671	1432.9310	3422.2328	118.7882	2337.2921	3509.2301	789.6380	257.1651	1017.5596	1670.4373	215.7542	1582.8346	868.0061	480.1287	595.0426	720.8430	355.8865	607.3304	870.2789	311.3743	2789.8201	1154.8881	1314.9027
YKVNEYQGPVAPSGGFFLFRPRN(-.98)	1397.2289	1780.6247	514.8561	1045.6669	1040.5740	181.7562	2214.3245	992.3815	797.3459	1001.9745	367.7714	364.9192	257.1317	664.0614	1312.6796	1906.0464	103.7824	2132.3606	961.7503	942.4899	555.2847	1077.3473	1782.3408	1186.5297	1199.7913	856.0402	1385.2345	702.9548	1094.0188	2703.3670	1101.3148	668.1617
Q(-17.03)EPGAPAAGM	1353.91954	1097.45020	2418.92268	652.63013	869.32176	381.90204	2133.38087	971.32698	11.00814	1293.02459	1146.17282	3029.91720	237.75127	2910.37191	3834.84299	899.07723	307.37824	973.36442	2525.07925	588.41284	1539.37877	1400.76172	789.07747	760.61290	2012.52217	643.37150	751.41303	1563.20745	750.29848	2569.06018	1051.89866	1368.81684
Q(-17.03)EPGAPAAGM(+15.99)	1793.1733	1071.9821	3223.6571	945.2703	804.2836	601.2279	1820.7497	263.2696	1067.3122	3113.6456	798.6134	1984.3290	948.5801	2149.4112	2890.0867	1655.6275	1095.6339	789.6461	1408.7340	603.1945	954.6112	1494.4603	825.7174	1271.6258	861.3999	242.4189	450.6964	1271.1949	584.9811	1912.4549	761.5464	572.5913
A(+42.01)AYKLVLIRH	1130.6869	2798.7301	1219.9993	2749.8439	627.4309	483.3605	588.0735	2883.4581	1279.9718	519.1375	580.0286	347.0306	958.6070	230.4244	890.2429	167.9425	844.8724	360.7447	324.7101	582.2745	1592.6898	1128.4528	944.8078	165.7048	491.1466	675.9818	644.5652	257.3025	299.4548	478.0842	203.7753	198.6627
A(+42.01)AYKLVLIRHGE	962.94646	1709.87070	249.91770	3667.10306	762.71765	100.71407	441.56558	2281.96655	1061.06447	66.08384	369.14582	208.92588	2039.95476	102.81385	872.99697	193.33632	718.62319	355.78043	211.25818	2204.53106	1643.96711	1100.62205	1618.94183	354.16586	618.43152	94.74124	431.17528	78.60883	83.46098	249.53941	536.48024	166.35961
SVNPYLQ(-.98)	861.0863	1133.2432	2804.9095	354.3044	1164.2967	395.5912	1818.7159	2474.0911	1225.3680	543.0904	1095.9493	2759.6245	366.9221	1543.7043	1686.3996	538.3506	498.8378	835.3087	2630.3353	632.0374	903.7555	642.6705	615.8387	369.2079	923.5098	402.1357	427.1679	840.9029	308.9180	983.7623	239.3260	1084.7844
SVNPYLQGKRLDNVV	1136.7172	1764.9882	2418.5940	981.9184	721.6108	442.6688	2376.7119	520.3494	1020.8072	1146.3761	739.3344	1170.5527	454.7664	2256.9844	4059.5081	827.5662	350.1608	670.8309	930.0564	622.8546	2299.1872	1135.1636	1220.3625	1233.2423	292.4447	599.6983	934.8150	552.0124	518.1111	3396.9080	1405.0847	1195.0940
SVNPYLQGKRLDNVVA	1458.1754	1204.2235	3443.2102	965.5780	767.0003	473.4469	2480.5397	1238.9487	1201.9355	884.5478	783.2166	1602.3546	447.7813	2222.7877	3743.6424	846.5899	483.4516	738.6864	1663.8227	433.6393	1779.9663	1231.7367	656.2566	679.8930	821.2722	770.6229	594.8650	800.9041	345.4969	1544.3163	1377.5302	741.2718
SVPHFSEEEKEPE	1621.8063	496.6782	4128.4777	874.5051	601.1604	330.3977	2481.2050	458.7201	1277.4479	621.4519	1229.7060	3207.7861	891.9415	3110.4415	3024.2923	711.8649	1008.0547	1102.7682	1632.4975	958.2405	1269.6327	452.5820	508.1130	539.8701	509.6546	565.9836	609.0940	1178.8916	355.8274	1677.9754	742.1111	1133.2732
VPHFSEEEKEPE	556.9006	815.5703	8720.0795	660.3865	921.9266	506.0518	2589.7625	800.5475	1707.2680	712.4423	1657.4476	1413.9127	227.6488	2146.7127	1889.4620	524.0519	1634.2973	957.7334	1993.0992	987.7303	400.2419	1252.4576	687.3197	530.0443	887.6660	401.8406	488.8097	595.4318	302.0400	1708.7992	587.7350	1287.3465
IKMALQQEGFD	1769.57725	1376.33786	373.01578	10242.89287	47.05449	521.62381	1028.89755	2578.28777	589.45486	608.05417	891.86439	893.99688	331.97509	979.69020	917.78997	665.51930	690.54742	196.79723	1151.26506	866.03578	680.48891	1121.25377	262.25859	297.64989	1009.91150	696.22183	397.79601	788.95306	723.54486	1241.87742	792.54359	946.08480
PMFIVNTNVPRA	1238.39392	2167.18936	467.74943	7004.94315	1521.11314	348.85928	26.91456	4945.96222	939.99257	111.76479	911.30271	635.27459	2527.43654	183.62449	1070.51707	902.28613	2146.58056	1016.21528	255.67268	4754.48279	1765.64521	2490.58267	188.51143	698.70543	993.88141	888.73370	595.88654	754.56892	319.69722	241.16807	913.51709	235.38004
TAYAIYMLAMAAFPKKQN	1550.99560	1580.39836	654.24231	4673.15936	2225.38457	383.30202	175.59878	4130.13199	1620.16197	920.43984	726.17127	1107.45584	4473.79235	805.30726	64.30082	892.66789	1799.38525	97.78010	1088.80995	4840.61074	4056.55182	4849.86942	3879.14502	1589.50332	3920.44357	622.89010	2121.60320	1673.44524	1465.94551	528.35591	2301.98947	961.40711
SAVISLEGKPL	1178.9738	1891.1703	632.0592	2839.5494	2565.9136	345.5368	770.1815	4071.6439	166.1772	705.9277	582.1840	441.4797	1407.4899	389.5594	958.1795	548.0644	845.2002	549.6326	395.1124	840.7462	1834.9788	1356.2887	836.8128	372.0013	983.7864	626.9566	721.9012	159.5931	223.4196	313.0009	754.0227	427.1928
FPKPEGSQD	1243.7404	592.0136	4528.2291	519.7172	2130.1338	355.0223	2197.8866	1323.1802	1539.2217	864.1189	848.7550	730.3689	928.5868	2608.0392	2311.9915	1492.7083	1411.4765	135.6791	800.3154	814.8332	1083.1008	778.8010	768.5480	496.0894	139.3005	660.2700	535.1208	316.7570	303.3757	1177.1758	371.3297	524.2587
FPKPEGSQDKSLHN	974.11770	435.55716	4774.31829	385.20068	711.19095	310.10488	1998.63905	517.32908	16.81933	493.76752	1137.16490	624.58035	803.36795	2426.10291	2997.63677	1387.19047	1420.02181	925.34208	1149.92103	164.82184	1431.39886	770.58766	1132.90329	818.50760	321.60727	1131.50997	622.83260	800.68355	353.58120	1268.67406	541.30093	561.74068
SLKPEFVDIINAKQ	1312.4377	4436.9343	694.9876	6800.9496	1620.3174	204.4521	788.4027	11771.3462	1011.8423	912.8782	586.6355	433.8873	2043.2146	625.4030	942.6093	218.2236	2128.4289	698.0052	422.8598	646.3211	2483.8293	2783.3499	2852.8205	731.1159	1652.1821	254.4416	542.5560	252.8342	903.2799	551.3805	73.5686	352.4842
GRTLYGFGG	1165.1965	2251.5875	1530.6159	2165.0778	1302.6051	367.8827	927.3360	2962.8787	1131.9325	191.6725	1203.8398	775.1868	835.1908	619.3790	350.4179	282.7213	850.8640	78.9446	542.7368	535.1921	1750.8438	1735.6190	788.6543	411.3374	872.9100	369.7414	531.4833	250.7511	378.5867	536.0139	401.6746	313.0106
RTLYGFGG	1190.57138	1582.37301	1434.01920	3344.26119	2272.53723	283.73569	220.16955	3503.24741	858.27250	309.17597	1245.30134	572.59477	1031.65783	550.96299	276.68447	124.86891	1037.03921	58.99698	334.81521	537.85760	1811.99038	1738.42667	854.03596	508.91184	1131.98790	339.21395	322.80038	177.99614	422.14510	467.75947	395.79647	505.54004
TLYGFGG	397.8480	746.7548	236.9991	3292.0774	2177.5969	185.6250	185.3785	3105.0090	3188.8519	275.2126	432.9219	209.3927	951.7851	165.5347	140.7230	150.4470	465.1513	211.8669	430.5752	1762.9758	1124.6496	886.2080	443.8803	193.2410	1022.6403	202.3814	211.9018	149.1161	245.4066	233.8977	150.3373	391.9125
MQIFVKTL	1409.97080	3661.18468	1007.04198	4114.37385	746.43352	510.71767	474.19641	5188.32212	1220.04548	440.47964	1087.95592	677.15255	678.90064	409.26938	280.06202	333.44831	481.67868	94.12112	664.63893	628.80350	1720.88314	1675.34445	579.08156	352.87131	536.55525	186.22950	494.30596	204.36628	447.36752	397.15928	225.26378	511.96313
LLRALASSNARAQQ	1778.7886	756.7937	174.3052	918.0838	841.1197	456.0759	2076.9113	915.1195	926.6981	377.3045	1034.2967	2166.3924	686.0243	2141.3909	2786.1964	826.0816	743.8947	432.6560	1630.1242	912.3070	1013.5384	983.5076	573.9832	711.3765	882.2147	1073.6794	419.0634	898.9169	352.8291	1809.5976	1079.2957	550.6347
AVDQDLGPEVPPENVL(-.98)	1376.3918	1652.6405	2155.2448	1352.7600	2797.9135	857.7210	2476.3980	2487.2989	1123.2057	3644.1989	1127.9554	2682.0792	992.5401	5796.8898	5642.5347	2822.5389	440.2493	994.3272	1956.6939	1068.8221	3888.5722	1572.6688	831.3143	2215.4831	1523.5655	869.8641	2479.7907	1356.1355	1489.5127	3666.5363	1685.8307	2211.5616
LENSSPQAPA	715.9376	788.1330	1293.0311	281.7965	1522.0447	401.9946	1718.1094	686.8244	1173.1739	600.1111	544.5075	1663.7815	744.3233	2567.9325	4121.3732	1060.6471	1002.7337	647.3608	1546.9273	1011.3563	1420.6429	1023.4015	841.9960	893.0261	207.0698	780.1970	525.3939	662.9971	287.1728	943.7927	299.0774	683.7254
LENSSPQAPARRLLPP	1196.83394	1366.38541	2234.10338	92.65545	760.72455	226.47525	2416.45900	703.53945	1124.20015	815.60209	772.95556	1417.54321	115.89976	2117.78560	4440.23115	782.80994	445.62666	635.71288	1209.98210	930.45302	1190.38518	834.12602	815.41336	814.57186	591.07096	689.00990	588.82014	719.39531	232.75541	2042.11439	1151.75252	699.21997
SLSAASAPLAETSTPL	1213.5055	1061.8173	2831.8125	730.7903	1936.9790	958.9634	2291.0264	1445.4632	1213.5611	1336.1552	1008.8449	3216.5646	671.7324	3596.0981	5613.5812	1650.6680	651.5720	1313.7602	1552.6675	1043.4800	1624.8444	1018.5592	816.2730	1104.3693	1218.8215	842.3472	935.2853	1055.7822	853.7529	2262.6801	1441.5462	1538.8658
SLSAASAPLAETSTPLRL	1821.9411	939.3980	3076.5749	331.2227	1832.4271	903.0306	2923.5353	1483.2780	1160.2203	2999.2267	992.0293	4971.2105	282.9144	3032.3178	6610.9992	1665.3757	757.3400	1262.2334	2309.1399	223.6952	1539.8381	943.1508	690.3911	650.1971	1536.0917	867.5319	678.8528	1468.4405	698.5226	2382.7367	1843.9269	1412.8791
SSEPEAAPAPRRL	2330.2087	1475.7988	501.0695	1395.8364	3032.3261	80.2223	984.2112	4026.0427	977.9581	191.0552	546.2145	490.7833	2054.0189	878.9290	970.9634	404.0801	819.7928	209.2990	537.4587	1389.2826	2206.1662	771.3588	1979.6622	673.1648	334.6738	428.0039	596.8494	308.6340	219.1378	1145.0571	265.2716	385.5026

Log2 transformation

Data transformation applies a mathematical transformation on individual values themselves. For mass spec data, log transformation is a good choice, as it reduces or removes the skewness of mass spec data.

Code

d1 <- fh[-1,]
anno = data.frame(Label = as.factor(t(fh)[, "class"]))
log2_d1 <- log2(type.convert(d1)) ## log2 transformation
# save data
#write.csv(log2_d1, file.path(WORKING_DIR, "log2_transformed.csv"))

# draw histogram
hist_log2 <- ggplot_truehist(unlist(log2_d1), "Log2 Transformed")
qq_log2 <- ggplot_carqq(unlist(log2_d1), "Log2 Transformed")
pca_log2 <- ggplot_pca(log2_d1, anno, "Label","Log2 Transformed")

Code

plot_grid(hist_log2, qq_log2, pca_log2, nrow = 1)

Code

rownames(log2_d1) = feature_name$name
# display table
log2_d1 %>% 
        knitr::kable(., digits = 3, "html") %>%
        kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
        kableExtra::scroll_box(width = "100%", height = "500px")

	Pituitary_08	Pituitary_02	Pituitary_19	Pituitary_29	Pituitary_04	Pituitary_11	Pituitary_34	Pituitary_06	Pituitary_18	Pituitary_16	Pituitary_31	Pituitary_36	Pituitary_07	Pituitary_22	Pituitary_27	Pituitary_25	Pituitary_32	Pituitary_30	Pituitary_35	Pituitary_09	Pituitary_05	Pituitary_10	Pituitary_12	Pituitary_13	Pituitary_28	Pituitary_03	Pituitary_21	Pituitary_26	Pituitary_15	Pituitary_24	Pituitary_17	Pituitary_23
DEGHDPVHESPVDTA	10.881	8.301	12.536	7.082	9.988	8.239	11.550	9.344	10.322	9.496	10.172	10.967	7.724	11.024	11.358	9.905	9.381	9.745	11.132	9.848	10.061	9.889	9.133	9.326	9.902	10.048	8.276	11.011	8.673	11.372	10.175	9.730
DPVHESPVDT	9.517	10.006	11.971	9.334	10.415	9.018	10.787	9.863	10.094	9.502	9.700	10.001	9.665	11.538	11.707	10.417	9.137	9.794	9.249	8.553	8.627	9.341	10.615	9.438	10.174	9.501	9.164	9.430	9.932	10.926	9.291	10.033
DPVHESPVDTA	10.287	8.075	12.622	8.298	9.622	8.692	10.707	10.025	10.631	9.002	9.508	10.032	9.741	10.745	11.223	9.387	10.172	9.936	9.491	10.002	9.151	9.972	9.949	9.000	10.299	9.208	8.332	8.526	8.420	10.646	8.260	10.364
ELDQLLHY	9.456	8.515	12.054	10.618	9.677	7.678	9.100	10.287	10.428	8.812	10.093	10.037	8.663	9.481	9.412	8.477	9.831	7.831	9.574	10.081	9.655	9.878	9.140	7.438	11.073	8.724	7.610	8.590	8.351	9.381	9.065	10.185
FNPYFDPL	9.983	10.198	12.213	9.344	11.613	9.374	11.636	11.380	9.271	10.866	10.055	11.829	10.169	10.982	11.465	10.919	9.565	9.654	11.439	9.130	11.758	11.053	10.269	9.307	11.463	10.172	10.238	10.840	10.799	11.243	10.915	10.677
GRGREPGAYPALDSRQE	10.373	9.534	11.349	8.538	9.516	8.143	10.670	9.153	10.088	8.823	10.232	9.346	9.683	10.020	10.868	9.676	10.290	9.395	10.199	7.142	9.085	9.663	9.894	9.567	8.965	9.624	9.061	10.089	8.428	10.530	9.581	9.184
LDEGHDPV	10.401	8.800	11.904	9.602	10.490	7.974	10.644	9.720	10.495	9.282	10.244	11.493	10.151	10.931	11.175	10.190	9.793	8.286	11.173	9.741	9.635	9.821	8.965	8.896	7.363	9.801	8.231	10.110	8.474	10.398	8.895	9.673
LDEGHDPVHESPV	10.595	9.345	12.907	9.592	9.588	7.367	11.599	9.757	10.914	10.083	7.630	11.633	10.010	10.507	11.762	10.161	10.083	8.228	11.738	10.045	9.377	9.417	7.841	8.700	10.545	10.058	9.644	11.398	7.661	11.187	10.034	10.178
LLDEGHDPVHESPVD	10.766	10.090	11.875	9.788	9.751	10.137	11.254	10.155	10.269	10.715	10.175	11.578	9.753	10.916	11.397	10.195	9.206	9.414	10.828	6.413	9.599	10.581	9.283	10.183	10.024	9.184	8.600	10.465	8.830	11.138	10.461	9.768
LLDEGHDPVHESPVDT	10.336	9.507	11.488	8.482	9.577	8.376	11.093	10.246	10.144	9.705	9.964	11.143	6.989	11.284	11.584	10.436	8.541	10.197	10.657	8.989	10.169	10.268	9.683	9.458	9.713	9.025	9.128	10.420	9.217	11.464	10.638	10.220
LLDEGHDPVHESPVDTA	10.831	9.984	12.087	8.604	8.998	8.787	11.376	10.140	10.426	10.118	10.435	12.264	8.841	11.932	12.353	10.392	9.691	9.576	11.412	8.325	9.635	10.399	9.097	9.494	9.944	9.178	9.051	10.632	8.849	11.039	10.808	9.759
NPYFDPLQWKNSDFE	11.177	10.626	13.170	9.990	12.833	11.769	11.818	11.557	10.999	13.129	10.456	12.149	10.875	13.795	13.220	13.145	11.514	12.981	11.473	12.437	12.745	12.005	12.605	12.440	12.529	12.138	11.984	11.747	13.155	12.933	12.128	12.155
PVHESPVDTA	11.168	9.651	11.203	8.276	10.589	4.973	10.952	10.356	10.471	9.910	10.294	9.926	9.955	11.115	11.354	10.051	7.939	9.854	10.948	9.860	9.861	9.251	9.635	8.732	6.861	8.915	7.168	9.364	8.528	9.939	9.270	9.580
Q(-17.03)KIAEKFSQ	9.342	8.937	11.116	10.042	9.634	7.514	10.263	10.608	10.401	8.561	9.946	10.156	9.185	9.620	10.348	9.681	7.298	8.456	10.357	9.944	9.216	7.920	7.634	2.845	8.867	8.640	7.533	9.596	7.658	10.130	9.418	9.632
Q(-17.03)KIAEKFSQR(-.98)	10.284	9.772	11.901	8.232	10.234	7.441	11.182	10.661	10.400	8.743	9.815	10.162	9.356	10.004	10.679	9.190	9.142	8.215	11.037	9.767	10.182	10.231	9.484	9.313	9.052	9.572	8.547	9.679	7.764	10.329	9.278	8.250
SEES(+79.97)QEKEY	10.349	9.469	11.883	8.226	11.055	8.798	11.012	9.446	10.257	10.148	9.656	11.094	10.030	12.442	11.554	11.469	10.766	8.828	11.349	7.725	11.343	9.751	10.822	10.460	7.177	10.488	9.602	9.978	8.228	10.437	9.073	9.214
PESAFSE	10.243	9.441	9.831	9.927	9.822	8.331	10.991	10.457	9.721	10.214	11.201	12.171	9.624	11.374	10.715	9.824	9.721	7.678	11.295	8.825	10.114	10.078	8.739	8.829	9.431	9.273	8.799	9.681	9.208	9.919	9.747	10.115
AGTQESVDSAKP	9.130	8.435	9.405	7.019	8.895	8.356	8.498	9.621	9.874	8.760	10.104	11.398	9.392	11.187	11.514	9.840	10.670	8.713	11.094	10.227	9.928	9.706	9.703	9.546	6.830	10.876	9.188	10.558	9.724	9.505	10.348	10.243
AGTQESVDSAKPRVY	9.620	9.257	10.053	7.871	9.098	9.119	10.337	9.704	4.071	8.906	11.006	12.121	9.266	11.271	11.184	9.993	10.050	8.873	11.132	7.373	9.479	10.247	9.343	9.657	8.933	10.213	8.674	11.010	8.964	10.247	10.802	10.345
EGFFRLT	10.265	10.936	10.011	11.194	10.232	7.429	9.521	10.997	9.961	6.926	9.424	8.813	9.946	9.077	8.172	7.169	9.502	9.085	9.100	9.775	11.055	10.589	9.792	8.276	9.878	8.728	8.778	7.831	9.117	9.086	8.309	9.122
ESVDSAKPRVY	10.497	10.576	10.042	10.470	10.148	8.657	9.843	10.776	10.071	8.091	10.314	9.511	9.772	11.348	9.684	7.633	10.162	9.486	10.334	10.548	12.433	10.901	10.902	9.965	10.965	9.898	9.612	8.934	9.364	10.989	8.932	10.844
GTQESVDSAKPRVY	10.708	10.803	10.292	10.791	10.582	8.595	10.642	11.781	10.147	8.564	10.897	11.341	10.033	11.135	10.162	9.595	10.426	8.691	10.691	9.875	11.218	10.987	10.309	9.977	9.977	9.807	9.099	9.326	8.789	10.143	9.560	9.462
LVQLAGTQESVDSAKPRVY	10.379	11.448	10.488	9.068	10.075	9.443	11.567	9.920	10.048	10.979	11.125	12.463	7.289	12.220	11.979	10.123	9.969	9.610	11.727	7.494	10.871	11.573	8.767	9.855	11.024	9.840	8.653	11.195	9.474	11.617	11.236	9.958
SVDSAKPRVY	10.112	10.504	10.275	10.348	11.475	6.769	9.291	11.020	6.358	8.075	9.584	9.294	11.441	10.305	8.245	9.772	10.088	2.706	10.340	9.666	11.543	10.470	10.991	9.493	6.976	8.888	8.534	8.345	8.127	8.695	6.953	7.594
TQESVDSAKPRVY	10.520	11.060	10.407	11.404	10.241	8.355	9.514	11.001	10.038	7.737	10.087	9.783	11.622	9.960	8.895	9.155	10.712	7.220	9.876	9.197	11.683	10.645	10.878	9.298	9.433	9.319	9.140	9.096	8.413	9.272	8.949	8.137
VQLAGTQESVDSAKP	10.193	10.302	10.105	9.045	9.610	9.228	9.480	10.290	9.754	9.634	10.704	11.846	9.737	10.520	11.093	9.223	9.882	8.992	10.903	8.441	9.108	10.303	9.475	8.632	10.140	9.679	8.904	10.494	9.144	10.875	11.383	9.896
VQLAGTQESVDSAKPR	6.610	10.560	9.854	9.855	9.233	8.894	10.719	10.109	9.944	9.410	10.249	11.690	9.741	11.114	11.619	9.260	10.425	9.626	11.819	8.287	10.088	10.097	9.639	10.003	9.901	10.643	8.684	11.190	8.722	11.272	11.026	10.192
VQLAGTQESVDSAKPRV	9.616	9.381	9.779	6.677	9.528	8.179	9.732	10.288	9.849	9.714	11.297	11.810	9.755	11.015	10.725	9.599	9.733	9.244	11.022	6.800	9.001	10.024	9.708	9.486	10.442	10.067	8.398	11.001	8.746	11.219	12.700	10.219
VQLAGTQESVDSAKPRVY	10.148	10.430	9.891	8.700	8.570	8.907	10.069	10.100	5.314	9.625	10.763	11.677	8.416	11.051	11.105	9.502	9.780	9.123	10.826	7.819	9.768	10.301	9.424	9.415	9.985	9.687	9.005	10.629	8.832	10.667	11.620	10.013
AEAFPLEF	10.805	10.395	8.305	9.898	9.585	8.300	10.351	10.504	9.520	11.923	9.008	11.192	11.936	11.632	9.326	9.228	10.957	9.332	11.121	12.493	12.495	11.816	9.741	11.543	11.601	7.669	9.598	9.376	8.420	10.533	9.849	10.966
AEEETAGGDGRPEPSP	10.094	8.600	10.407	8.018	9.615	9.700	10.826	8.834	5.654	9.805	10.208	11.691	9.914	12.128	11.431	10.276	10.261	9.905	10.674	8.631	10.235	9.435	9.209	9.793	9.327	10.287	9.884	10.504	9.139	10.294	9.069	9.860
AEEETAGGDGRPEPSPR	10.620	8.699	8.656	8.673	9.861	9.680	10.957	9.429	6.593	9.732	9.807	10.511	10.212	12.195	11.349	10.312	10.815	10.101	10.678	7.594	11.164	10.308	10.144	10.513	8.409	9.915	10.010	10.154	9.088	9.732	9.162	9.125
AEEETAGGDGRPEPSPRE(-.98)	10.298	8.771	10.100	8.278	9.766	8.972	11.048	9.055	5.850	9.559	9.889	10.309	10.269	12.376	11.530	10.370	10.445	9.959	10.298	7.557	10.847	9.515	9.778	10.221	8.370	9.909	9.816	10.033	8.467	9.451	8.754	9.224
EAFPLEF	10.679	10.631	8.063	12.203	11.691	9.165	8.515	12.369	11.968	11.536	10.656	10.354	12.074	3.939	9.114	10.150	10.902	10.313	10.317	12.760	11.720	11.357	9.693	10.378	11.268	10.156	11.113	9.989	10.625	9.510	9.326	9.313
EEETAGGDGRPEPSPRE(-.98)	10.375	8.549	10.765	8.326	10.293	9.322	11.094	9.742	5.652	9.622	10.135	9.977	10.546	12.315	11.696	10.418	10.555	10.160	9.859	7.344	10.821	9.665	10.166	10.295	7.822	10.637	9.706	10.287	8.717	9.648	9.205	9.642
EETAGGDGRPEPSPRE(-.98)	9.914	8.885	10.859	6.868	9.793	5.728	10.704	7.922	2.901	9.711	8.312	8.860	7.865	11.490	11.596	9.908	9.721	7.052	9.190	4.712	11.369	8.163	9.285	9.945	5.742	9.430	9.516	7.372	8.208	8.279	8.118	9.115
ELEGEQPD	11.064	9.066	8.504	10.245	10.447	3.785	11.110	11.034	7.348	10.404	10.175	10.556	9.911	11.684	10.528	10.611	8.272	9.950	10.750	10.055	11.547	10.692	10.103	9.679	7.339	9.085	9.253	9.451	9.093	9.688	9.085	8.614
FMTSEKSQTP	10.224	8.242	8.556	8.996	10.417	9.206	11.893	10.542	9.398	10.219	8.821	11.019	9.770	10.410	10.798	9.380	9.167	8.937	9.000	7.222	10.824	10.455	9.389	9.859	9.620	8.314	10.060	9.774	7.947	10.271	8.524	8.575
GPYRVEHF	11.302	9.717	10.414	10.548	10.181	7.597	10.457	10.670	9.952	9.372	9.761	9.596	9.937	9.478	8.857	8.796	9.707	7.776	10.095	10.345	10.413	11.221	8.824	8.844	10.021	8.685	8.649	8.827	8.052	9.426	8.610	7.613
GPYRVEHFRWGNPPKD	11.278	10.456	9.867	9.949	9.667	4.991	10.847	7.111	9.632	10.070	10.167	6.447	9.558	6.506	9.046	9.460	10.436	10.061	9.756	8.514	6.211	10.623	9.734	9.945	9.506	10.838	10.877	11.090	9.425	10.748	11.372	8.364
KYVMGHF	10.503	9.890	9.922	9.847	10.114	8.488	11.047	10.471	8.765	9.045	10.161	10.217	9.386	9.325	9.394	8.678	9.146	7.667	10.308	8.060	10.174	11.067	8.631	8.934	9.217	8.650	8.836	9.298	8.219	10.181	9.179	9.019
KYVMGHFRWD	10.913	9.635	5.769	8.526	3.901	8.112	11.000	9.753	5.885	9.360	10.009	8.521	7.849	7.707	10.141	9.137	8.696	8.975	10.267	8.212	8.860	10.162	8.494	8.748	9.448	9.788	9.179	10.507	8.767	11.038	11.147	8.708
R(+42.01)PVKVYPNVAENESAEAFPLEF	9.962	8.928	9.234	8.725	7.031	9.709	12.678	9.365	9.548	11.797	10.589	10.067	10.596	11.684	10.910	12.831	11.703	9.953	13.646	10.915	11.104	11.327	10.951	9.960	11.523	10.439	12.287	13.297	10.870	12.898	12.113	12.212
RAEEETAGGDGRPEPSPRE(-.98)	9.436	7.980	8.972	7.549	8.376	8.233	11.636	9.911	5.973	9.544	9.333	8.820	11.887	11.979	11.979	11.606	11.453	10.192	10.304	9.347	10.327	10.397	10.079	11.233	7.717	11.047	9.815	10.629	8.384	9.166	8.746	7.498
RPVKVYPNVAENE	11.085	9.220	10.486	10.979	10.659	7.364	10.666	11.671	8.434	9.315	9.192	9.459	11.136	9.158	8.584	8.380	8.512	7.804	9.016	10.285	10.781	9.998	8.652	8.013	10.007	7.608	7.759	7.734	6.344	9.577	7.742	6.507
RPVKVYPNVAENES(+79.97)AEAFPLE	11.472	9.742	7.704	10.291	9.216	10.111	12.068	11.090	7.121	12.052	10.876	11.344	10.344	12.061	11.214	10.868	10.808	10.867	10.478	10.022	11.932	11.587	9.699	11.402	11.188	10.266	12.005	10.956	11.011	11.457	11.255	10.528
RPVKVYPNVAENES(+79.97)AEAFPLEF	11.242	9.547	6.443	9.853	10.833	11.517	11.716	10.750	8.116	12.987	11.029	12.436	11.755	9.566	8.247	11.888	11.658	11.681	12.521	11.644	14.088	12.663	12.929	13.853	13.050	13.083	14.476	13.393	14.247	13.920	13.510	13.243
RPVKVYPNVAENESAEAFPLE	11.367	10.277	8.099	10.477	7.962	8.952	12.116	10.822	9.700	10.845	10.709	10.619	9.996	10.904	10.817	10.218	10.120	10.345	10.123	9.023	10.661	11.240	9.097	9.583	10.856	9.449	10.324	9.950	9.712	10.716	10.687	9.962
RPVKVYPNVAENESAEAFPLEF	10.907	9.787	6.324	10.045	8.876	9.801	11.334	10.437	6.864	11.514	10.304	10.978	10.433	11.259	11.127	10.669	10.084	11.011	11.490	9.976	11.316	11.547	10.654	11.106	11.971	10.841	11.938	11.557	11.288	12.226	11.287	11.272
RRPVKVYPNVAENESAEAFPLEF	10.746	10.115	9.549	10.230	10.308	10.043	10.834	10.706	9.858	10.011	11.134	10.464	10.222	10.299	10.233	10.746	10.218	10.788	10.666	10.269	9.568	11.549	10.391	10.257	11.090	10.551	9.600	11.021	10.270	11.085	11.162	9.821
S(+42.01)MEHFRWGKPV(-.98)	11.237	10.555	6.317	9.153	8.428	7.017	10.088	10.402	9.632	8.808	10.460	9.222	8.743	8.917	10.624	8.996	9.003	8.358	9.875	7.689	10.396	10.349	8.487	8.990	10.180	10.149	9.560	10.136	9.179	10.196	10.903	7.918
S(+42.01)YSMEHF	10.708	10.554	6.358	10.183	8.821	9.993	10.165	10.790	7.128	10.267	10.689	11.637	8.885	10.520	9.966	8.719	9.465	8.246	9.331	9.113	11.515	12.025	9.191	9.743	10.038	8.436	10.548	8.759	10.074	10.630	9.881	8.348
S(+42.01)YSMEHFRWGKPV(-.98)	10.380	10.457	6.505	8.515	8.384	7.688	10.802	10.198	5.182	9.964	10.348	9.487	8.690	9.637	10.726	8.957	9.322	9.322	9.391	8.351	10.796	10.623	9.484	9.707	10.247	9.952	10.456	9.929	9.505	10.516	10.778	7.768
SAEAFPLEF	10.620	9.608	5.738	12.461	11.617	7.694	8.449	12.402	12.394	8.032	9.217	8.304	11.721	9.847	8.075	8.935	8.595	7.275	9.059	12.454	11.672	11.237	9.880	8.025	11.455	9.400	8.775	7.803	8.120	7.638	7.047	8.411
SMEHFRWGKPV(-.98)	10.347	10.935	7.713	9.863	9.601	8.547	11.511	6.944	9.644	10.426	9.632	9.974	9.345	9.577	10.901	9.526	8.363	9.704	10.438	6.237	9.689	10.855	8.128	7.809	9.707	8.605	9.065	10.232	8.185	10.968	10.562	8.722
SYSMEHF	9.960	9.571	10.655	9.414	8.763	8.596	9.444	10.630	9.848	10.049	10.003	11.879	8.720	11.220	10.310	9.305	9.449	9.348	8.486	8.510	11.862	12.441	10.169	10.419	11.063	8.064	9.727	8.681	8.409	11.044	10.625	8.082
SYSMEHFRWGKPV(-.98)	10.360	9.356	8.677	7.558	5.545	6.845	10.869	9.094	5.241	9.753	9.389	8.758	8.532	9.135	10.157	8.719	9.320	9.571	9.360	8.537	10.084	10.380	9.310	10.064	10.156	8.377	10.022	9.347	7.869	11.437	11.500	8.195
TPLVTLF	10.160	9.753	5.047	12.406	11.106	7.847	8.828	12.567	11.896	9.740	8.169	8.387	10.934	8.015	9.773	8.866	8.424	8.829	7.276	11.954	11.382	11.822	8.493	8.845	8.541	9.470	8.494	8.853	8.423	10.278	10.364	9.431
TPLVTLFKNAIIKN	11.197	9.445	9.982	11.750	9.775	6.661	8.596	12.304	10.256	7.998	8.178	9.117	10.941	6.269	9.723	7.927	8.302	6.062	8.629	11.471	10.616	10.213	9.191	6.398	11.193	7.282	8.651	6.675	7.479	7.419	7.175	6.252
Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLF	14.273	8.847	6.271	11.777	13.873	15.124	13.733	13.970	12.234	17.029	12.150	15.590	13.657	15.439	14.501	14.875	13.055	12.868	12.797	14.711	15.177	13.664	11.540	15.748	10.521	12.093	13.892	10.935	15.917	13.000	12.629	13.413
Y(+42.01)GGFMTSEK	10.854	8.849	9.790	12.342	10.109	6.753	8.311	12.113	10.087	7.753	9.912	7.002	10.467	7.863	5.664	8.525	7.690	8.935	8.441	11.211	10.229	10.717	8.042	5.778	10.621	6.320	8.095	7.031	7.444	6.286	9.749	7.805
Y(+42.01)GGFMTSEKSQ	11.574	10.674	10.099	12.928	10.555	7.496	10.120	12.257	10.025	7.964	8.851	8.103	10.703	7.713	9.644	8.609	8.665	3.449	9.637	9.610	11.246	11.509	9.181	7.552	10.399	7.538	8.952	7.687	7.568	7.576	6.150	8.201
Y(+42.01)GGFMTSEKSQTP	11.204	9.052	8.469	9.888	9.858	10.631	12.057	11.462	10.107	12.584	11.554	12.637	11.223	12.319	12.380	10.720	10.694	10.340	10.973	10.113	11.342	11.187	10.081	11.185	10.328	9.724	11.349	10.821	10.677	10.685	10.668	10.754
Y(+42.01)GGFMTSEKSQTPL	10.885	9.798	9.696	8.951	9.888	11.287	11.766	11.934	9.982	12.042	11.064	12.059	9.057	11.276	11.489	10.754	10.174	10.373	10.608	10.159	11.219	11.596	9.021	10.277	11.475	10.351	10.291	10.715	11.217	11.048	10.940	11.399
Y(+42.01)GGFMTSEKSQTPLV	10.714	9.799	8.091	10.175	10.195	11.434	11.651	11.709	9.285	12.086	10.382	12.323	9.635	11.912	11.499	10.312	10.335	10.821	10.789	10.164	12.018	11.809	9.134	11.038	10.960	10.564	11.667	10.583	11.478	10.337	11.254	10.766
Y(+42.01)GGFMTSEKSQTPLVT	11.128	9.867	6.553	9.311	8.962	11.097	11.366	11.242	8.165	11.696	10.762	12.264	9.629	12.011	11.415	10.524	10.070	10.796	10.415	9.078	11.854	10.868	9.868	10.559	11.963	10.308	11.192	10.764	11.256	12.041	11.117	11.336
Y(+42.01)GGFMTSEKSQTPLVTL	11.303	9.658	8.217	9.998	10.922	12.172	12.282	12.342	9.437	13.457	11.658	12.712	10.896	13.128	12.470	12.196	12.160	12.470	12.123	10.957	13.426	13.608	11.487	12.902	13.029	13.035	13.985	13.576	14.144	13.524	13.432	13.931
Y(+42.01)GGFMTSEKSQTPLVTLF	14.306	11.380	7.079	12.604	15.361	17.564	15.219	16.096	12.874	18.482	14.545	17.065	15.599	17.307	15.428	16.387	15.174	15.649	15.418	16.284	18.131	18.452	13.549	17.712	13.116	13.891	16.853	15.980	19.540	17.574	16.910	17.085
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIK	12.119	9.999	9.298	9.110	9.002	12.351	12.184	12.397	9.936	14.050	13.008	13.267	12.326	13.314	12.780	13.106	13.330	14.549	13.598	12.537	14.061	11.722	11.865	12.810	14.693	13.108	13.919	12.525	14.288	14.010	14.159	13.871
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKN	11.816	10.406	8.732	9.384	9.812	12.409	12.172	12.093	8.572	13.872	12.320	13.030	12.102	12.911	12.746	13.334	12.589	12.795	13.296	11.504	14.419	13.499	12.418	13.688	14.368	13.161	14.096	12.549	14.669	14.545	14.595	14.427
Y(+42.01)SMEHFRWGKPV(-.98)	11.328	9.850	5.870	8.962	8.065	8.364	11.484	10.413	9.783	10.022	10.028	9.943	8.971	9.310	10.661	9.218	8.165	8.977	9.847	8.965	9.688	10.078	8.718	8.254	9.702	9.048	9.537	10.537	8.559	10.677	10.672	8.969
YGGFMTSEKSQTPLVT	9.752	8.259	10.520	6.295	7.215	7.846	9.253	10.987	9.466	10.023	9.903	10.301	8.806	11.803	10.542	9.201	10.311	9.288	11.324	10.738	10.755	12.109	10.118	10.024	12.150	8.506	10.492	10.977	8.020	10.809	10.298	8.942
YGGFMTSEKSQTPLVTL	11.115	9.926	12.941	8.730	10.289	10.053	10.896	11.538	11.169	11.487	11.244	13.083	10.977	12.802	12.849	10.704	9.379	9.583	11.891	9.832	11.467	11.154	9.430	10.350	10.831	8.978	8.992	9.187	10.664	12.055	12.028	10.474
YGGFMTSEKSQTPLVTLF	10.188	10.345	8.951	9.781	9.700	11.127	11.471	10.541	9.944	14.253	10.756	12.951	10.673	13.130	11.061	12.027	10.636	11.989	12.029	10.646	13.445	13.754	11.552	14.286	12.437	13.151	13.414	12.842	13.263	13.783	13.976	12.559
YGGFMTSEKSQTPLVTLFKNAIIKN	9.647	9.963	9.572	9.964	9.987	9.241	8.865	10.664	9.605	12.570	10.535	10.750	9.402	11.202	10.054	10.314	10.022	12.062	11.664	10.470	11.486	9.566	10.467	11.870	13.664	9.652	11.233	8.098	9.244	12.932	12.992	10.439
LPGLLLF	11.354	11.966	8.651	10.560	13.421	9.013	8.542	13.223	13.527	9.837	9.642	8.638	12.582	11.219	9.074	10.278	9.776	10.136	8.593	13.674	13.850	11.374	12.349	11.487	9.293	10.478	12.207	10.991	11.735	11.154	11.129	10.963
LPHLPGLLLF	11.350	11.674	9.673	10.108	11.476	8.233	9.808	11.837	11.906	11.150	9.415	7.996	11.840	8.133	10.239	10.929	9.996	9.510	10.299	14.005	13.652	12.268	13.087	13.265	10.304	12.461	13.209	8.551	12.532	12.864	10.708	11.861
TPLPELFDRVVML	6.924	6.311	11.735	14.740	10.805	6.311	8.736	12.102	15.562	7.103	6.973	7.992	13.856	9.635	10.066	7.463	11.128	10.576	8.086	13.760	11.369	12.646	10.969	11.536	14.042	13.140	10.179	11.986	11.698	12.555	13.043	10.493
F(+42.01)PAMPLSSLFANAVLRAQHLH	11.740	12.690	9.251	12.068	11.579	11.754	12.286	12.383	11.196	12.764	10.964	11.279	13.333	11.731	8.559	13.599	10.813	11.988	13.869	12.273	10.949	13.134	12.487	11.644	10.866	9.522	13.700	12.466	13.027	14.013	11.267	11.871
FPAM(+15.99)PLSSLFANAVLR	11.669	11.880	9.638	11.027	12.181	11.317	7.953	11.620	10.280	12.457	10.150	11.184	13.267	11.145	9.794	11.820	12.994	10.218	11.204	13.099	14.857	12.330	14.092	13.727	11.520	9.902	13.803	12.493	13.896	11.027	11.935	10.241
FPAM(+15.99)PLSSLFANAVLRAQH	10.703	12.377	8.078	11.183	9.936	11.317	10.481	11.143	10.241	10.881	9.727	9.727	12.210	9.501	8.718	9.328	12.466	8.494	8.804	12.001	13.078	11.577	12.603	12.347	9.214	8.863	12.302	10.770	10.996	10.219	8.555	11.199
FPAM(+15.99)PLSSLFANAVLRAQHLH	10.542	11.962	9.327	9.357	10.833	11.005	7.708	10.156	9.801	11.050	10.442	9.872	11.799	7.601	8.567	8.229	11.770	9.595	9.971	11.124	12.064	12.212	13.244	12.811	10.729	9.894	12.581	8.736	12.034	10.967	8.768	8.452
FPAMPLS	10.061	11.262	8.832	11.502	12.209	8.394	7.968	11.943	10.274	7.736	8.124	8.976	10.893	8.622	7.413	7.792	8.036	5.549	9.069	10.519	10.941	10.206	10.266	8.052	9.454	7.316	9.315	7.199	7.905	8.743	7.428	8.135
FPAMPLSS	10.235	12.140	10.519	11.262	10.945	9.423	9.068	11.330	9.870	7.734	9.673	10.774	10.423	9.736	9.156	7.653	9.175	7.140	9.617	9.888	11.825	11.253	10.759	9.267	9.179	8.655	10.590	8.531	9.498	10.212	9.206	8.582
FPAMPLSSL	10.330	11.869	10.902	11.360	11.853	10.468	8.635	11.918	7.977	9.574	10.090	11.156	11.049	10.014	9.197	9.217	9.667	8.037	10.080	9.875	12.195	11.381	11.119	10.017	10.350	9.787	10.916	9.196	10.098	10.377	10.040	9.392
FPAMPLSSLF	10.527	11.853	9.158	12.087	12.858	9.582	7.521	12.726	11.165	9.232	9.819	10.231	12.782	10.243	8.414	8.693	10.598	7.036	9.323	11.694	13.170	11.413	11.911	10.770	10.064	9.473	11.590	6.709	11.755	10.154	8.609	9.254
FPAMPLSSLFA	10.845	12.896	11.528	12.424	13.869	12.005	8.380	12.912	9.972	11.643	11.849	12.569	14.011	12.769	10.733	10.060	12.963	9.656	11.121	12.606	15.981	14.042	15.078	14.512	12.654	13.310	15.692	10.548	15.965	14.216	12.698	12.561
FPAMPLSSLFAN	10.945	12.594	11.281	12.367	13.617	11.866	8.898	12.985	9.901	11.306	11.287	12.318	13.534	12.865	10.653	10.129	12.957	9.739	11.557	12.551	15.711	13.988	15.032	13.887	12.343	12.564	15.193	10.310	15.001	14.000	12.389	12.424
FPAMPLSSLFANA	10.850	12.441	10.916	12.091	13.214	5.405	11.710	12.827	9.558	13.011	11.323	12.599	13.823	8.724	11.223	11.020	13.059	12.072	11.976	12.787	16.294	10.698	15.763	14.938	13.485	13.738	16.055	10.399	16.307	14.795	13.407	14.124
FPAMPLSSLFANAVLR	11.005	12.752	8.591	12.422	12.225	11.305	8.388	12.563	10.398	11.361	11.466	11.824	14.351	11.294	10.003	10.283	13.079	9.913	11.862	13.192	16.158	14.934	15.967	15.410	13.229	14.017	16.661	11.289	16.826	14.909	13.544	12.804
FPAMPLSSLFANAVLRA	11.908	12.581	5.090	12.743	12.138	9.673	7.148	13.557	10.164	9.201	10.027	9.654	13.047	8.011	6.287	8.393	9.643	5.850	10.593	12.618	13.103	12.019	10.307	12.168	11.055	10.444	13.518	8.097	13.970	11.546	9.716	9.922
FPAMPLSSLFANAVLRAQ	12.843	12.340	4.768	13.710	13.356	12.106	9.536	14.031	10.976	12.694	13.418	14.798	16.376	13.642	12.852	12.641	15.247	12.720	14.051	15.019	15.990	14.981	14.686	15.922	12.350	13.929	16.431	10.840	16.800	14.294	13.297	11.588
FPAMPLSSLFANAVLRAQH	10.619	12.564	6.586	11.943	10.615	10.342	7.939	11.424	7.012	10.035	10.549	9.980	12.257	8.792	8.432	8.918	11.731	9.266	10.862	11.691	13.343	13.226	13.795	12.721	11.305	12.035	13.901	9.320	13.667	13.089	11.662	10.541
FPAMPLSSLFANAVLRAQHLH	10.712	12.641	6.298	11.742	8.044	10.697	8.296	11.120	8.196	10.009	11.041	10.308	11.919	7.008	8.863	9.482	12.303	9.880	10.864	11.735	12.285	13.640	13.684	12.890	11.538	11.822	13.897	9.568	13.803	12.976	11.714	10.410
FPAMPLSSLFANAVLRAQHLHQ	10.854	12.712	6.318	11.759	8.608	10.771	8.981	11.483	9.479	10.560	11.097	10.582	12.484	8.296	9.395	10.172	12.170	10.272	11.281	11.631	12.705	13.795	14.350	13.411	12.269	12.111	14.281	10.078	13.939	13.369	12.123	10.732
FPAMPLSSLFANAVLRAQHLHQL	11.597	13.265	5.796	12.620	7.268	11.602	9.114	12.463	10.104	11.527	12.210	11.588	12.930	5.650	8.734	11.103	13.313	10.745	12.318	13.545	12.578	14.769	13.717	14.167	12.296	12.977	15.366	10.948	15.944	14.332	13.086	12.543
FPAMPLSSLFANAVLRAQHLHQLA	11.927	13.516	5.694	12.845	8.046	11.646	8.887	12.510	10.492	11.599	12.504	11.664	13.247	6.562	8.564	11.024	13.234	10.783	12.156	13.562	12.875	14.938	14.109	14.518	12.957	13.081	15.458	11.108	15.706	14.505	13.248	12.505
FPAMPLSSLFANAVLRAQHLHQLAAD	11.911	12.857	4.739	13.730	9.107	10.822	8.894	13.686	11.009	10.753	11.041	10.679	13.845	6.497	7.297	10.791	12.550	8.838	12.217	14.051	13.183	14.759	14.083	14.529	12.948	12.036	15.161	11.292	15.313	13.950	12.383	11.674
GPVQFLSRIF	10.311	10.698	10.897	12.176	11.453	8.397	8.975	12.488	13.531	8.895	7.316	9.173	11.664	9.538	10.066	6.288	8.212	8.973	7.946	13.002	10.244	10.139	10.881	9.132	9.906	10.011	7.949	9.063	8.874	9.031	9.424	7.647
PVQFLSRIF	9.651	9.640	10.540	11.423	11.950	7.729	8.564	12.740	13.253	6.572	6.354	8.444	11.400	8.638	9.796	8.071	7.899	8.594	7.225	12.326	9.685	9.655	9.910	6.982	9.147	9.303	8.047	5.563	6.243	8.610	9.161	9.284
SPRIGQILK	10.381	10.583	10.219	11.832	12.393	8.355	5.807	12.058	10.693	5.631	5.501	8.651	11.322	9.180	9.845	8.330	8.824	8.279	8.181	10.194	10.052	9.101	9.775	7.727	9.046	6.512	7.938	5.616	6.113	6.458	3.561	8.743
SPRIGQILKQ	9.662	9.026	3.814	11.652	12.092	4.292	4.999	12.222	12.127	5.547	4.539	7.119	10.782	3.477	3.703	5.814	6.672	7.293	6.620	10.850	8.510	8.225	8.548	5.522	8.517	5.013	5.390	3.484	4.256	4.758	8.308	5.970
TDMELLRF	8.346	7.354	10.532	11.011	11.818	5.608	6.980	11.578	12.570	5.637	9.529	7.057	10.270	9.470	10.007	6.694	8.081	8.218	7.139	9.701	7.917	6.927	7.330	3.897	8.230	9.825	5.256	3.411	8.760	7.214	3.214	4.888
ASLDKFLASVSTVLTSKYR	10.803	13.570	9.190	11.064	11.272	10.884	11.654	10.865	10.053	11.538	12.321	11.257	10.381	11.776	10.822	11.845	13.271	10.688	9.585	12.094	12.806	14.604	12.364	11.972	13.153	14.021	13.113	8.600	13.905	11.337	12.884	7.699
FLASVSTVLTSKYR	9.835	12.836	10.246	10.927	9.121	9.647	11.321	11.179	10.031	10.816	11.782	11.146	9.549	10.304	8.098	10.315	11.541	8.249	8.091	9.715	12.048	12.545	10.724	9.279	10.881	10.228	10.397	10.162	10.362	9.713	11.723	8.299
SLDKFLA	8.873	8.211	5.790	11.165	11.860	4.696	3.040	11.627	11.971	4.011	2.264	4.153	10.042	4.999	4.997	5.377	6.716	7.531	6.214	11.661	8.261	7.470	6.895	2.877	9.516	4.630	2.479	2.254	3.255	7.285	4.610	5.107
SLDKFLASVSTVLTSKYR	10.477	12.848	3.790	11.117	6.627	10.691	10.985	10.445	9.516	5.968	12.165	9.680	11.280	4.927	10.540	7.028	13.398	9.071	9.525	12.053	14.183	14.758	13.236	11.788	12.612	12.587	13.005	9.528	13.374	11.633	12.539	9.585
SVSTVLTSKYR	9.666	11.802	8.671	10.572	9.660	8.381	6.261	10.461	9.883	4.578	10.602	9.752	11.499	9.943	6.554	5.865	11.220	6.018	8.967	9.886	12.635	11.719	10.988	8.797	9.316	9.400	8.996	7.682	8.621	8.606	8.880	9.299
TPAMHASLDKFLASVSTVLTSKYR	10.295	12.688	8.875	8.142	10.848	11.034	11.298	11.833	9.219	8.140	12.946	9.891	10.554	12.381	11.369	12.188	14.259	9.988	9.690	8.292	12.028	14.339	12.832	11.432	12.048	12.947	12.348	7.149	13.453	10.130	11.784	9.500
VHLTDAEKAAVN	10.394	11.137	10.337	10.621	10.789	8.866	7.588	11.347	10.210	6.265	10.827	10.792	10.503	10.087	7.531	7.136	11.095	7.686	9.833	10.715	11.728	10.810	10.708	8.892	9.651	10.101	8.964	8.501	8.673	9.594	8.694	8.147
VHLTDAEKAAVNG	10.416	10.495	9.605	10.644	10.446	7.566	7.495	11.380	9.742	9.155	10.122	9.883	9.024	8.845	7.021	6.732	10.686	7.159	9.367	9.781	10.974	10.986	9.962	8.623	9.117	9.563	8.224	7.863	7.999	8.839	7.924	7.458
AAFQKVVAGVASALAHKYH	10.394	12.364	9.894	10.762	10.876	8.577	4.668	10.908	9.968	9.139	10.225	7.560	10.758	9.781	9.882	5.773	10.847	6.448	8.650	10.555	10.150	11.780	10.847	8.599	10.314	10.482	10.010	8.542	9.696	9.882	10.541	8.116
AFQKVVAGVASALAHKYH	10.066	12.232	10.032	8.200	8.221	8.581	9.790	10.742	10.008	9.356	10.260	8.351	10.856	3.873	9.892	9.440	10.915	7.017	8.356	9.662	10.216	11.415	10.690	8.649	9.925	10.107	9.327	7.672	9.054	8.967	9.472	6.201
AQAAFQKVVAGVASALAHKYH	10.446	10.416	9.723	10.129	9.844	8.910	9.405	9.264	9.772	5.567	9.657	8.090	10.643	9.825	10.074	9.444	10.828	9.347	6.452	8.578	9.594	11.351	10.297	8.333	9.967	10.036	8.795	8.098	8.662	8.960	9.476	5.361
ASALAHKYH	9.119	9.944	8.964	10.843	10.219	7.671	9.480	10.687	10.143	9.294	9.928	9.237	11.341	8.561	9.901	7.158	10.751	5.107	8.456	9.488	10.654	10.314	10.245	8.427	8.616	9.564	9.432	8.989	8.008	8.574	7.767	7.209
GVASALAHKYH	10.031	10.900	9.289	10.748	10.213	8.045	6.849	10.901	5.952	6.069	10.225	8.697	11.316	8.949	7.232	6.957	10.854	7.057	8.800	10.265	11.133	10.572	10.568	8.836	9.232	10.163	8.541	8.036	8.524	8.875	8.246	6.987
QAAFQKVVAGVASALAHKYH	10.556	12.521	10.214	10.904	8.892	9.318	10.127	10.825	9.992	9.293	10.207	8.352	11.156	6.516	9.901	6.466	11.334	7.271	7.370	9.575	9.924	11.232	10.550	8.927	9.833	10.029	9.054	6.496	9.025	9.024	9.289	6.866
VHLTDAEKA	9.596	11.515	8.686	9.556	10.374	4.663	8.896	9.381	9.767	5.554	9.459	9.478	7.547	10.284	6.829	6.979	7.277	8.626	8.131	7.772	12.071	8.907	9.338	7.575	6.512	9.767	8.716	7.092	8.865	8.226	7.202	7.238
GPNLVARSKEALA	10.160	11.332	9.982	11.797	9.708	7.839	7.484	11.473	9.970	6.078	10.017	8.141	10.588	8.107	7.009	6.020	11.061	5.863	9.880	9.482	11.076	11.467	10.386	8.915	10.281	9.922	8.801	8.046	8.839	8.446	9.188	7.438
AKVAVLGA	11.607	10.986	8.726	12.692	13.137	7.628	8.232	12.564	9.903	7.450	9.232	9.571	11.762	8.902	6.475	8.634	10.449	9.200	8.686	10.524	9.752	10.534	10.379	8.990	8.761	8.021	7.566	9.328	7.901	7.420	5.739	8.199
SNRVVDLMAYMASKE	10.341	11.474	9.935	12.820	9.606	9.124	4.947	11.371	9.639	6.940	10.470	9.201	10.992	9.948	9.965	8.175	11.083	9.715	10.030	9.763	12.192	11.915	11.170	9.152	10.002	7.936	9.852	8.546	9.112	9.762	8.423	9.778
VKVGVNGF	9.858	10.312	10.597	11.797	10.653	8.886	7.467	11.390	9.681	7.958	9.825	10.534	9.650	9.521	8.472	8.328	10.045	9.705	9.176	9.426	10.590	10.940	10.157	8.631	10.688	8.214	8.519	7.820	8.371	9.827	7.309	10.055
VKVGVNGFGRIG	10.259	11.444	9.153	11.233	9.528	7.284	6.627	11.020	9.797	9.168	9.972	8.918	9.916	8.940	9.872	7.063	9.717	9.248	9.290	9.677	10.998	10.957	10.202	8.810	10.133	7.988	9.089	8.345	7.685	9.998	8.248	8.982
PHFLYPK	10.887	9.560	7.192	9.534	7.826	8.406	10.927	10.444	8.159	8.792	10.377	10.117	9.154	9.448	10.444	9.313	9.612	8.151	10.741	8.297	9.914	10.750	8.916	8.812	10.387	9.795	8.701	10.214	8.324	10.360	10.318	8.948
SQENPNTYSEDLDV	11.562	10.471	10.138	10.754	11.703	11.372	11.259	10.753	9.867	11.515	12.345	14.155	11.367	13.802	13.262	11.779	10.515	10.792	12.373	9.145	12.094	11.537	9.343	12.225	12.549	10.131	12.420	12.097	10.913	12.609	11.263	12.160
YGGFLRKYP	10.167	10.588	6.567	8.598	8.765	8.635	10.948	9.402	9.760	9.284	10.205	10.778	7.781	9.921	10.896	8.962	9.019	8.499	11.269	8.280	9.726	10.320	9.220	8.778	9.282	9.027	9.104	10.035	8.603	10.656	10.203	8.842
YGGFLRKYPK	10.320	10.011	10.231	8.746	8.768	7.388	11.195	9.934	9.897	7.884	9.651	9.133	9.265	9.126	10.852	8.435	9.875	8.190	11.013	7.401	9.652	10.049	9.616	9.297	7.874	9.302	8.964	9.931	8.163	10.442	9.577	7.745
YGGFLRRI	10.249	10.475	9.895	8.716	8.412	8.461	10.719	9.462	7.680	9.762	9.857	10.203	8.713	8.691	10.343	9.240	9.106	9.044	10.963	8.552	9.292	10.235	9.387	9.152	9.001	9.280	9.267	10.019	8.854	10.597	10.705	9.704
YGGFLRRQFKVVT	10.678	11.310	10.360	7.221	9.896	8.892	10.891	9.567	9.715	9.284	9.646	9.784	8.348	7.482	10.235	9.080	9.195	8.882	10.986	8.828	9.502	11.004	9.388	9.227	9.846	9.231	9.365	10.322	8.981	10.683	10.489	8.962
MDQLAKELT	10.628	9.657	10.143	10.403	10.333	6.309	10.288	11.126	10.186	9.145	8.709	9.679	9.416	10.989	11.568	9.912	8.105	8.914	9.530	9.795	10.583	9.938	9.766	9.266	8.203	7.812	8.728	8.927	7.637	10.740	8.245	8.811
MDQLAKELTAE	9.895	10.421	10.732	10.121	10.116	9.038	10.897	10.398	10.151	9.861	9.517	10.177	8.459	11.120	11.450	10.173	10.193	8.850	12.352	9.959	10.679	9.984	9.672	8.196	9.760	9.647	9.201	8.979	8.309	11.251	10.163	10.477
WSRMDQLAKELT	9.783	10.084	9.601	9.909	10.664	7.377	11.386	10.210	9.953	10.344	8.585	9.725	9.677	11.174	11.968	10.230	9.304	10.037	8.845	9.212	10.527	9.655	10.056	9.803	9.554	8.571	9.431	8.422	8.928	11.088	9.571	8.507
WSRMDQLAKELTAE	10.757	10.127	10.842	10.162	10.187	9.259	12.246	10.407	10.285	11.268	10.018	10.121	10.356	11.676	12.629	10.920	7.835	10.727	10.866	9.912	11.295	10.206	10.461	10.432	10.558	10.498	10.355	10.142	9.885	12.033	11.731	9.836
NQEQAEQGREHL	10.509	9.968	10.348	9.969	11.162	6.400	11.435	8.906	10.164	9.663	9.639	9.390	8.892	12.723	12.422	11.081	8.275	9.873	8.982	8.438	11.899	7.306	10.489	11.064	8.954	8.841	9.234	8.298	7.312	9.823	7.557	8.971
Q(-17.03)ELGKLTGPSNQ	10.600	10.687	10.599	10.021	10.333	7.625	10.669	10.094	9.959	10.112	8.377	10.031	9.367	10.835	11.831	9.752	9.144	9.236	9.690	9.764	10.901	10.082	8.873	9.529	9.431	9.032	10.207	9.266	8.416	10.412	9.427	8.980
YEENSRENPF	10.597	9.419	11.915	8.425	10.060	7.296	11.749	10.553	10.409	10.527	10.485	11.741	6.892	11.191	11.777	9.625	8.007	9.991	10.706	7.753	10.628	9.762	8.907	9.217	9.494	8.475	9.246	9.765	8.283	11.446	10.174	10.361
YKVNEYQGPVAPSGGFFLFRPRN(-.98)	10.448	10.798	9.008	10.030	10.023	7.506	11.113	9.955	9.639	9.969	8.523	8.511	8.006	9.375	10.358	10.896	6.697	11.058	9.910	9.880	9.117	10.073	10.800	10.213	10.229	9.742	10.436	9.457	10.095	11.401	10.105	9.384
Q(-17.03)EPGAPAAGM	10.403	10.100	11.240	9.350	9.764	8.577	11.059	9.924	3.460	10.337	10.163	11.565	7.893	11.507	11.905	9.812	8.264	9.927	11.302	9.201	10.588	10.452	9.624	9.571	10.975	9.330	9.553	10.610	9.551	11.327	10.039	10.419
Q(-17.03)EPGAPAAGM(+15.99)	10.808	10.066	11.654	9.885	9.652	9.232	10.830	8.040	10.060	11.604	9.641	10.954	9.890	11.070	11.497	10.693	10.098	9.625	10.460	9.236	9.899	10.545	9.690	10.312	9.751	7.921	8.816	10.312	9.192	10.901	9.573	9.161
A(+42.01)AYKLVLIRH	10.143	11.451	10.253	11.425	9.293	8.917	9.200	11.494	10.322	9.020	9.180	8.439	9.905	7.848	9.798	7.392	9.723	8.495	8.343	9.186	10.637	10.140	9.884	7.372	8.940	9.401	9.332	8.007	8.226	8.901	7.671	7.634
A(+42.01)AYKLVLIRHGE	9.911	10.740	7.965	11.840	9.575	6.654	8.786	11.156	10.051	6.046	8.528	7.707	10.994	6.684	9.770	7.595	9.489	8.475	7.723	11.106	10.683	10.104	10.661	8.468	9.272	6.566	8.752	6.297	6.383	7.963	9.067	7.378
SVNPYLQ(-.98)	9.750	10.146	11.454	8.469	10.185	8.628	10.829	11.273	10.259	9.085	10.098	11.430	8.519	10.592	10.720	9.072	8.962	9.706	11.361	9.304	9.820	9.328	9.266	8.528	9.851	8.652	8.739	9.716	8.271	9.942	7.903	10.083
SVNPYLQGKRLDNVV	10.151	10.785	11.240	9.939	9.495	8.790	11.215	9.023	9.995	10.163	9.530	10.193	8.829	11.140	11.987	9.693	8.452	9.390	9.861	9.283	11.167	10.149	10.253	10.268	8.192	9.228	9.869	9.109	9.017	11.730	10.456	10.223
SVNPYLQGKRLDNVVA	10.510	10.234	11.750	9.915	9.583	8.887	11.276	10.275	10.231	9.789	9.613	10.646	8.807	11.118	11.870	9.726	8.917	9.529	10.700	8.760	10.798	10.266	9.358	9.409	9.682	9.590	9.216	9.645	8.433	10.593	10.428	9.534
SVPHFSEEEKEPE	10.663	8.956	12.011	9.772	9.232	8.368	11.277	8.841	10.319	9.279	10.264	11.647	9.801	11.603	11.562	9.475	9.977	10.107	10.673	9.904	10.310	8.822	8.989	9.076	8.993	9.145	9.251	10.203	8.475	10.713	9.535	10.146
VPHFSEEEKEPE	9.121	9.672	13.090	9.367	9.849	8.983	11.339	9.645	10.737	9.477	10.695	10.465	7.831	11.068	10.884	9.034	10.674	9.903	10.961	9.948	8.645	10.291	9.425	9.050	9.794	8.650	8.933	9.218	8.239	10.739	9.199	10.330
IKMALQQEGFD	10.789	10.427	8.543	13.322	5.556	9.027	10.007	11.332	9.203	9.248	9.801	9.804	8.375	9.936	9.842	9.378	9.432	7.621	10.169	9.758	9.410	10.131	8.035	8.217	9.980	9.443	8.636	9.624	9.499	10.278	9.630	9.886
PMFIVNTNVPRA	10.274	11.082	8.870	12.774	10.571	8.447	4.750	12.272	9.877	6.804	9.832	9.311	11.303	7.521	10.064	9.817	11.068	9.989	7.998	12.215	10.786	11.282	7.559	9.449	9.957	9.796	9.219	9.560	8.321	7.914	9.835	7.879
TAYAIYMLAMAAFPKKQN	10.599	10.626	9.354	12.190	11.120	8.582	7.456	12.012	10.662	9.846	9.504	10.113	12.127	9.653	6.007	9.802	10.813	6.611	10.089	12.241	11.986	12.244	11.922	10.634	11.937	9.283	11.051	10.709	10.518	9.045	11.169	9.909
SAVISLEGKPL	10.203	10.885	9.304	11.471	11.325	8.433	9.589	11.991	7.377	9.463	9.185	8.786	10.459	8.606	9.904	9.098	9.723	9.102	8.626	9.716	10.842	10.405	9.709	8.539	9.942	9.292	9.496	7.318	7.804	8.290	9.558	8.739
FPKPEGSQD	10.280	9.209	12.145	9.022	11.057	8.472	11.102	10.370	10.588	9.755	9.729	9.512	9.859	11.349	11.175	10.544	10.463	7.084	9.644	9.670	10.081	9.605	9.586	8.954	7.122	9.367	9.064	8.307	8.245	10.201	8.537	9.034
FPKPEGSQDKSLHN	9.928	8.767	12.221	8.589	9.474	8.277	10.965	9.015	4.072	8.948	10.151	9.287	9.650	11.244	11.550	10.438	10.472	9.854	10.167	7.365	10.483	9.590	10.146	9.677	8.329	10.144	9.283	9.645	8.466	10.309	9.080	9.134
SLKPEFVDIINAKQ	10.358	12.115	9.441	12.732	10.662	7.676	9.623	13.523	9.983	9.834	9.196	8.761	10.997	9.289	9.881	7.770	11.056	9.447	8.724	9.336	11.278	11.443	11.478	9.514	10.690	7.991	9.084	7.982	9.819	9.107	6.201	8.461
GRTLYGFGG	10.186	11.137	10.580	11.080	10.347	8.523	9.857	11.533	10.145	7.582	10.233	9.598	9.706	9.275	8.453	8.143	9.733	6.303	9.084	9.064	10.774	10.761	9.623	8.684	9.770	8.530	9.054	7.970	8.564	9.066	8.650	8.290
RTLYGFGG	10.217	10.628	10.486	11.707	11.150	8.148	7.782	11.774	9.745	8.272	10.282	9.161	10.011	9.106	8.112	6.964	10.018	5.883	8.387	9.071	10.823	10.764	9.738	8.991	10.145	8.406	8.334	7.476	8.722	8.870	8.629	8.982
TLYGFGG	8.636	9.544	7.889	11.685	11.089	7.536	7.534	11.600	11.639	8.104	8.758	7.710	9.894	7.371	7.137	7.233	8.862	7.727	8.750	10.784	10.135	9.792	8.794	7.594	9.998	7.661	7.727	7.220	7.939	7.870	7.232	8.614
MQIFVKTL	10.461	11.838	9.976	12.006	9.544	8.996	8.889	12.341	10.253	8.783	10.087	9.403	9.407	8.677	8.130	8.381	8.912	6.556	9.376	9.296	10.749	10.710	9.178	8.463	9.068	7.541	8.949	7.675	8.805	8.634	7.815	9.000
LLRALASSNARAQQ	10.797	9.564	7.445	9.842	9.716	8.833	11.020	9.838	9.856	8.560	10.014	11.081	9.422	11.064	11.444	9.690	9.539	8.757	10.671	9.833	9.985	9.942	9.165	9.474	9.785	10.068	8.711	9.812	8.463	10.821	10.076	9.105
AVDQDLGPEVPPENVL(-.98)	10.427	10.691	11.074	10.402	11.450	9.744	11.274	11.280	10.133	11.831	10.139	11.389	9.955	12.501	12.462	11.463	8.782	9.958	10.934	10.062	11.925	10.619	9.699	11.113	10.573	9.765	11.276	10.405	10.541	11.840	10.719	11.111
LENSSPQAPA	9.484	9.622	10.337	8.139	10.572	8.651	10.747	9.424	10.196	9.229	9.089	10.700	9.540	11.326	12.009	10.051	9.970	9.338	10.595	9.982	10.472	9.999	9.718	9.803	7.694	9.608	9.037	9.373	8.166	9.882	8.224	9.417
LENSSPQAPARRLLPP	10.225	10.416	11.125	6.534	9.571	7.823	11.239	9.458	10.135	9.672	9.594	10.469	6.857	11.048	12.116	9.613	8.800	9.312	10.241	9.862	10.217	9.704	9.671	9.670	9.207	9.428	9.202	9.491	7.863	10.996	10.170	9.450
SLSAASAPLAETSTPL	10.245	10.052	11.468	9.513	10.920	9.905	11.162	10.497	10.245	10.384	9.978	11.651	9.392	11.812	12.455	10.689	9.348	10.359	10.601	10.027	10.666	9.992	9.673	10.109	10.251	9.718	9.869	10.044	9.738	11.144	10.493	10.588
SLSAASAPLAETSTPLRL	10.831	9.876	11.587	8.372	10.840	9.819	11.513	10.535	10.180	11.550	9.954	12.279	8.144	11.566	12.691	10.702	9.565	10.302	11.173	7.805	10.589	9.881	9.431	9.345	10.585	9.761	9.407	10.520	9.448	11.218	10.849	10.464
SSEPEAAPAPRRL	11.186	10.527	8.969	10.447	11.566	6.326	9.943	11.975	9.934	7.578	9.093	8.939	11.004	9.780	9.923	8.658	9.679	7.709	9.070	10.440	11.107	9.591	10.951	9.395	8.387	8.741	9.221	8.270	7.776	10.161	8.051	8.591

EigenMS normalization

After batch correction, missing value imputation and log transformation, the data was normalized using EigenMS to account for sample-to-sample variability. EigenMS normalization preserves the treatment group differences in the data by estimating treatment effects with an ANOVA model, then uses singular value decomposition on the model residual matrix to identify and remove the bias.

Code

norm_d1 <- do_normalization_short(log2_d1, labels_d1)

hist_eigenms <- ggplot_truehist(unlist(norm_d1[-1,]), "log2-EigenMS")
qq_eigenms <- ggplot_carqq(unlist(norm_d1[-1,]), "log2-EigenMS")
pca_eigenms <- ggplot_pca(norm_d1[-1,], anno, "Label","log2-EigenMS")

Code

plot_grid(hist_eigenms, qq_eigenms, pca_eigenms, nrow = 1)

Code

norm_d1_mod = norm_d1[-1,] %>%
        as.data.frame() %>%
        rownames_to_column(., var = "rowname") %>%
        mutate(across(-rowname, as.numeric)) %>%
        column_to_rownames(., var = "rowname")
#write.csv(norm_d1_mod, file = "after_norm_data.csv")

#rownames(norm_d1_mod) == feature_name$variable
rownames(norm_d1_mod) = feature_name$name

# display table
norm_d1_mod %>% 
        knitr::kable(., digits = 3, "html") %>%
        kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
        kableExtra::scroll_box(width = "100%", height = "500px")

	Pituitary_08	Pituitary_02	Pituitary_19	Pituitary_29	Pituitary_04	Pituitary_11	Pituitary_34	Pituitary_06	Pituitary_18	Pituitary_16	Pituitary_31	Pituitary_36	Pituitary_07	Pituitary_22	Pituitary_27	Pituitary_25	Pituitary_32	Pituitary_30	Pituitary_35	Pituitary_09	Pituitary_05	Pituitary_10	Pituitary_12	Pituitary_13	Pituitary_28	Pituitary_03	Pituitary_21	Pituitary_26	Pituitary_15	Pituitary_24	Pituitary_17	Pituitary_23
DEGHDPVHESPVDTA	10.784	8.534	10.489	7.977	9.713	8.756	10.156	9.492	11.211	9.471	9.596	10.321	9.015	9.000	9.808	9.156	9.614	9.553	10.719	11.543	10.121	10.278	9.770	9.788	10.351	10.277	9.189	10.841	9.868	10.996	10.596	9.609
DPVHESPVDT	9.427	10.133	10.215	9.694	9.873	9.347	9.917	9.788	10.218	9.613	9.327	9.690	10.447	10.237	10.709	9.878	9.285	9.625	9.074	9.521	8.771	9.785	11.121	9.907	10.503	9.750	10.049	9.406	10.890	10.841	9.774	9.927
DPVHESPVDTA	10.041	8.022	10.528	8.259	8.634	9.221	9.952	9.568	10.262	9.367	9.165	9.824	10.298	9.528	10.277	8.970	10.298	9.945	9.413	10.723	9.200	10.429	10.445	9.701	10.632	9.611	9.507	8.738	9.641	10.731	9.006	10.388
ELDQLLHY	8.890	8.001	10.415	9.771	8.317	8.604	8.912	9.150	9.458	9.580	9.967	10.039	8.440	8.949	8.959	8.655	9.848	8.421	9.668	10.016	9.249	9.909	9.281	8.290	11.239	9.272	8.743	9.275	9.599	9.687	9.979	10.615
FNPYFDPL	9.649	10.053	11.349	9.855	11.619	10.241	10.733	11.200	10.351	10.989	9.689	11.210	10.817	9.612	10.390	10.813	9.697	10.060	11.039	10.288	11.236	10.731	10.416	9.608	11.688	10.358	10.598	10.956	11.676	10.811	11.124	10.966
GRGREPGAYPALDSRQE	10.149	9.519	10.001	8.979	9.236	8.768	9.760	9.012	10.705	8.949	9.852	8.866	10.413	8.646	9.801	9.350	10.433	9.526	9.903	8.233	8.882	9.719	10.226	9.967	9.250	9.851	9.703	10.143	9.377	10.264	9.940	9.286
LDEGHDPV	9.944	8.561	9.814	9.585	9.536	8.945	9.727	9.054	10.514	9.765	9.837	11.082	10.720	9.428	9.991	9.943	9.933	8.643	10.966	10.689	9.308	9.949	9.365	9.684	7.711	10.276	9.404	10.477	9.946	10.331	9.684	9.940
LDEGHDPVHESPV	10.240	9.224	10.936	9.799	8.851	8.199	10.605	9.314	11.164	10.423	7.199	11.176	10.727	8.941	10.538	9.813	10.239	8.449	11.486	11.137	9.144	9.586	8.273	9.372	10.900	10.452	10.692	11.622	9.002	11.041	10.695	10.348
LLDEGHDPVHESPVD	10.570	10.173	10.275	10.539	9.583	10.806	10.038	10.170	11.247	10.750	9.675	10.933	10.801	9.127	10.015	9.696	9.401	9.463	10.414	7.915	9.422	10.687	9.731	10.589	10.389	9.400	9.304	10.410	9.916	10.735	10.804	9.813
LLDEGHDPVHESPVDT	10.191	9.658	9.950	9.398	9.560	8.993	9.796	10.398	11.324	9.651	9.437	10.442	8.154	9.405	10.139	9.875	8.750	10.181	10.195	10.628	10.024	10.377	10.151	9.795	10.090	9.193	9.755	10.278	10.246	10.989	10.900	10.219
LLDEGHDPVHESPVDTA	10.570	10.060	10.296	9.576	8.912	9.664	9.879	10.176	11.800	10.141	9.824	11.424	10.119	9.733	10.653	9.837	9.928	9.705	10.865	10.206	9.331	10.416	9.592	9.944	10.374	9.419	9.807	10.565	10.139	10.481	11.163	9.861
NPYFDPLQWKNSDFE	11.450	11.055	12.712	10.854	13.249	11.487	11.172	12.214	11.766	12.736	10.209	11.862	11.699	12.990	12.630	12.575	11.633	12.479	11.250	13.337	13.104	12.345	12.944	12.301	12.707	12.003	12.016	11.293	13.195	12.648	11.956	11.804
PVHESPVDTA	10.503	9.227	8.886	8.166	9.475	6.349	9.896	9.409	10.614	10.570	9.825	9.383	10.512	9.346	9.949	9.930	8.092	10.506	10.669	10.955	9.244	9.178	9.997	9.691	7.242	9.510	8.495	9.919	10.333	9.813	10.210	10.056
Q(-17.03)KIAEKFSQ	8.826	8.601	8.452	9.476	8.003	8.441	9.583	9.569	9.443	9.310	9.610	10.027	9.476	8.382	9.363	9.442	7.405	8.781	10.376	10.430	9.056	8.346	8.124	3.913	9.229	9.288	9.161	10.168	9.355	10.412	10.569	9.883
Q(-17.03)KIAEKFSQR(-.98)	9.565	9.219	10.282	8.003	9.360	8.879	10.391	9.661	10.681	9.405	9.462	9.647	9.619	8.614	9.557	9.352	9.244	9.083	10.766	10.591	9.344	9.837	9.606	10.120	9.311	10.097	9.518	10.306	9.298	10.164	10.041	8.873
SEES(+79.97)QEKEY	9.954	9.385	9.985	8.912	10.715	9.863	9.622	9.197	11.364	10.353	9.080	10.308	11.109	10.337	9.914	11.061	10.981	9.146	10.857	9.416	10.901	9.689	11.259	11.047	7.590	10.828	10.492	10.096	9.671	9.972	9.579	9.450
PESAFSE	9.732	9.108	8.885	10.218	9.638	9.493	10.120	9.982	10.715	10.519	10.841	11.528	10.116	9.985	9.610	9.892	9.840	8.349	10.895	9.907	9.358	9.586	8.809	9.267	9.647	9.561	9.263	9.995	10.289	9.511	10.089	10.592
AGTQESVDSAKP	9.112	8.548	9.231	7.708	9.333	8.616	7.875	9.915	10.945	8.569	9.868	10.958	9.991	10.334	10.860	9.638	10.767	8.736	10.778	11.121	9.758	9.542	9.810	9.485	6.955	10.825	9.114	10.377	9.950	9.102	10.206	10.259
AGTQESVDSAKPRVY	9.439	9.417	9.896	9.733	10.439	10.086	8.652	10.390	7.294	8.438	10.373	10.815	10.797	8.944	9.388	9.650	10.303	9.209	10.197	9.837	8.722	9.484	9.480	9.461	9.231	10.076	8.302	10.618	9.591	9.038	10.349	10.576
EGFFRLT	9.266	9.798	9.748	9.643	9.017	9.001	10.006	9.193	9.062	8.042	9.578	8.865	8.765	9.390	8.323	8.285	9.379	10.617	9.235	8.924	9.764	9.631	9.268	9.052	9.755	9.313	9.334	9.040	10.132	9.377	9.090	10.203
ESVDSAKPRVY	9.730	9.769	10.089	9.809	9.731	10.028	9.857	9.666	10.313	8.755	10.306	9.226	9.218	11.123	9.444	8.438	10.120	10.729	10.195	10.473	11.250	9.911	10.476	10.401	10.907	10.245	9.769	9.710	10.129	10.871	9.295	11.718
GTQESVDSAKPRVY	9.844	9.983	9.623	10.214	9.901	10.205	10.311	10.547	10.441	9.329	10.739	10.914	9.747	10.372	9.503	10.244	10.440	9.962	10.475	10.177	10.004	10.104	10.056	10.641	10.047	10.285	9.634	10.157	10.006	9.987	10.153	10.360
LVQLAGTQESVDSAKPRVY	10.098	11.499	9.366	10.331	10.524	10.443	10.015	10.133	12.153	10.856	10.512	11.428	8.612	9.981	10.244	9.716	10.206	9.908	11.024	9.577	10.307	11.210	9.084	10.076	11.392	9.953	8.966	11.056	10.541	10.801	11.294	10.172
SVDSAKPRVY	8.485	8.840	10.085	9.092	10.564	9.731	9.118	8.707	7.006	9.467	9.484	8.598	10.466	9.538	7.512	11.358	10.033	5.294	9.985	9.757	9.060	8.438	10.185	10.474	6.919	9.652	8.992	9.952	9.908	8.389	7.775	9.414
TQESVDSAKPRVY	9.408	9.851	10.199	10.076	9.228	10.232	9.755	9.189	9.704	8.845	10.149	9.596	10.601	9.912	8.759	10.329	10.620	8.958	9.846	8.711	10.123	9.426	10.306	10.071	9.339	9.909	9.613	10.329	9.579	9.350	9.675	9.361
VQLAGTQESVDSAKP	9.930	10.175	9.628	9.469	9.726	9.914	8.812	10.176	10.708	9.703	10.437	11.347	10.206	9.511	10.299	9.196	9.977	9.350	10.573	9.327	8.646	9.964	9.533	8.808	10.288	9.792	9.069	10.577	9.747	10.501	11.475	10.150
VQLAGTQESVDSAKPR	6.480	10.612	9.341	10.614	9.574	9.413	9.861	10.291	11.199	9.298	9.913	11.104	10.490	9.890	10.672	9.032	10.557	9.771	11.416	9.461	9.781	9.874	9.800	10.074	10.095	10.673	8.782	11.076	9.246	10.790	10.999	10.296
VQLAGTQESVDSAKPRV	9.540	9.545	9.272	7.818	10.156	8.707	8.603	10.710	11.626	9.447	10.862	11.038	10.810	9.445	9.519	9.236	9.909	9.316	10.477	8.382	8.689	9.769	9.928	9.466	10.686	10.031	8.396	10.736	9.273	10.545	12.546	10.270
VQLAGTQESVDSAKPRVY	9.857	10.408	9.983	10.193	9.759	9.979	8.680	10.518	8.216	9.315	10.244	10.499	9.580	9.100	9.586	9.412	9.979	9.673	9.989	9.879	8.857	9.381	9.403	9.261	10.198	9.596	8.589	10.425	9.408	9.579	11.220	10.392
AEAFPLEF	10.139	9.629	9.458	9.492	9.868	9.526	10.553	9.717	10.380	12.338	9.103	10.847	11.275	11.758	9.358	10.220	10.879	10.627	10.901	12.348	11.176	10.471	9.062	11.581	11.402	7.786	9.077	9.996	8.618	10.211	9.756	11.865
AEEETAGGDGRPEPSP	10.066	8.885	9.870	9.612	10.567	10.271	9.353	9.520	8.064	9.367	9.645	10.684	11.340	10.113	9.888	9.756	10.494	9.908	9.954	10.722	9.899	9.132	9.502	9.682	9.637	10.183	9.784	10.073	9.704	9.386	8.780	9.862
AEEETAGGDGRPEPSPR	10.380	8.683	8.723	9.918	10.848	10.570	9.796	9.779	9.008	9.474	9.373	9.531	11.185	10.566	10.081	10.233	10.982	10.555	9.981	9.314	10.410	9.548	10.131	10.386	8.588	9.840	9.669	9.982	9.571	8.827	8.831	9.438
AEEETAGGDGRPEPSPRE(-.98)	10.039	8.824	9.385	9.638	10.475	9.949	9.553	9.372	8.210	9.352	9.309	9.238	11.554	10.244	9.878	10.043	10.671	10.299	9.556	9.631	10.207	8.999	9.995	10.302	8.687	9.943	9.880	9.847	9.343	8.549	8.647	9.464
EAFPLEF	10.328	9.803	10.084	10.162	11.124	9.209	10.520	11.203	9.988	12.197	11.451	11.300	9.866	6.636	11.139	11.535	10.553	11.194	10.991	10.116	11.175	10.635	8.752	10.316	10.686	10.230	10.511	10.830	9.763	10.274	9.358	9.921
EEETAGGDGRPEPSPRE(-.98)	10.159	8.657	9.847	9.687	10.905	10.215	9.568	10.086	7.873	9.409	9.538	8.942	11.890	10.143	10.018	9.995	10.790	10.393	9.144	9.430	10.300	9.293	10.462	10.414	8.169	10.685	9.880	10.070	9.633	8.795	9.153	9.809
EETAGGDGRPEPSPRE(-.98)	9.258	8.694	9.685	8.692	10.668	7.680	8.419	8.007	6.510	9.639	7.419	7.143	9.645	8.144	8.981	9.660	10.053	7.977	8.024	7.845	10.017	7.084	9.498	10.249	6.219	9.617	9.727	7.361	9.844	6.888	8.118	9.767
ELEGEQPD	10.062	8.202	8.279	10.380	10.519	5.899	10.234	10.034	9.161	10.983	9.825	9.569	10.067	10.173	9.283	11.337	8.359	11.557	10.126	11.236	9.810	9.215	9.723	10.157	7.449	9.461	9.368	10.182	10.405	8.971	9.359	9.742
FMTSEKSQTP	9.757	7.923	8.130	9.394	10.553	10.303	11.118	10.209	10.669	10.414	8.513	10.352	10.201	9.195	9.828	9.542	9.268	9.638	8.565	8.259	10.000	9.794	9.337	10.115	9.768	8.496	10.208	10.020	8.763	9.772	8.657	9.069
GPYRVEHF	10.353	8.739	9.803	9.513	9.189	9.237	10.455	9.133	9.675	10.329	9.725	9.373	9.267	9.134	8.510	9.643	9.665	9.181	10.034	10.159	9.154	10.321	8.471	9.607	10.023	9.241	9.268	9.854	9.259	9.480	9.340	8.607
GPYRVEHFRWGNPPKD	11.352	10.481	10.610	10.076	10.104	4.866	11.013	7.313	9.966	9.912	10.250	6.439	9.451	6.798	9.273	9.592	10.407	10.084	9.725	8.408	6.142	10.407	9.563	9.682	9.405	10.686	10.432	10.993	9.028	10.641	11.078	8.373
KYVMGHF	9.777	9.255	9.372	9.699	9.801	9.949	10.498	9.595	9.554	9.580	9.929	9.651	9.411	8.323	8.564	9.147	9.199	8.750	9.975	8.717	9.064	10.220	8.446	9.431	9.322	9.010	9.195	9.900	9.297	9.849	9.575	9.784
KYVMGHFRWD	10.866	9.638	8.141	10.000	6.121	8.638	10.464	10.614	9.132	8.688	9.876	7.585	8.358	7.116	9.677	9.536	8.752	9.552	9.528	9.437	7.868	8.726	7.918	7.852	9.290	9.275	7.500	10.109	8.032	9.877	9.959	9.088
R(+42.01)PVKVYPNVAENESAEAFPLEF	10.406	9.500	10.700	10.491	9.011	9.330	11.983	10.798	12.131	10.826	10.381	9.389	11.601	10.982	10.408	12.482	11.819	9.566	13.073	12.253	11.099	10.907	10.891	9.053	11.520	9.849	11.025	12.444	10.012	11.984	11.013	11.923
RAEEETAGGDGRPEPSPRE(-.98)	9.233	7.989	9.176	8.804	9.432	9.047	10.534	10.316	8.388	9.247	8.926	7.867	12.830	10.448	10.789	11.535	11.612	10.611	9.621	11.007	9.605	9.637	10.044	11.050	7.874	10.937	9.395	10.425	8.764	8.265	8.360	7.786
RPVKVYPNVAENE	9.787	7.902	9.322	9.514	9.111	9.595	10.608	9.519	7.863	10.671	9.114	9.184	10.273	8.593	8.039	9.451	8.466	9.660	8.963	10.054	9.147	8.912	8.262	9.158	10.053	8.422	8.800	9.154	8.132	9.716	8.860	7.822
RPVKVYPNVAENES(+79.97)AEAFPLE	11.377	9.722	9.437	11.557	10.985	10.679	11.481	11.746	9.896	11.537	10.705	10.490	10.867	11.347	10.654	11.164	10.876	11.395	9.819	11.202	11.055	10.391	9.268	10.742	11.103	9.890	10.733	10.657	10.580	10.457	10.346	10.880
RPVKVYPNVAENES(+79.97)AEAFPLEF	11.792	9.860	11.333	11.214	14.099	10.733	12.428	12.399	11.047	11.746	11.441	12.120	11.472	10.992	9.371	12.577	11.525	11.747	12.119	11.519	13.607	11.184	11.879	12.009	12.453	11.997	11.415	12.555	11.657	12.950	11.399	13.245
RPVKVYPNVAENESAEAFPLE	11.076	10.051	8.729	11.014	8.682	9.712	11.661	10.830	11.261	10.787	10.552	10.031	10.243	10.237	10.278	10.519	10.169	10.961	9.703	9.792	9.873	10.392	8.830	9.442	10.850	9.395	9.848	10.016	9.874	10.138	10.395	10.387
RPVKVYPNVAENESAEAFPLEF	10.971	9.859	8.941	11.447	11.148	10.070	11.007	11.401	9.895	10.773	10.257	10.188	10.817	10.998	10.924	11.067	10.110	11.449	10.842	10.944	10.483	10.206	10.050	10.105	11.759	10.261	10.137	11.092	10.298	11.155	10.014	11.553
RRPVKVYPNVAENESAEAFPLEF	10.713	10.097	9.838	10.448	10.609	10.174	10.719	10.802	10.359	9.933	11.098	10.302	10.315	10.152	10.116	10.807	10.231	10.904	10.543	10.491	9.391	11.322	10.312	10.153	11.077	10.494	9.386	10.982	10.220	10.903	11.013	9.899
S(+42.01)MEHFRWGKPV(-.98)	10.655	9.958	7.490	9.321	9.146	8.253	9.888	9.977	11.277	8.988	10.408	8.599	8.538	8.532	10.273	9.789	8.991	9.541	9.444	8.153	9.071	8.954	7.894	8.888	10.054	10.164	8.882	10.528	9.374	9.586	10.615	8.737
S(+42.01)YSMEHF	10.039	9.850	8.318	10.576	10.083	11.465	9.952	10.452	9.546	10.350	10.653	10.803	8.636	10.138	9.607	9.765	9.443	9.726	8.734	9.731	9.806	10.124	8.349	9.419	9.826	8.338	9.381	9.148	10.061	9.746	9.294	9.369
S(+42.01)YSMEHFRWGKPV(-.98)	9.903	10.043	8.496	9.621	10.181	8.990	10.153	10.401	8.429	9.703	10.154	8.388	9.005	8.733	9.986	9.684	9.377	10.520	8.582	9.639	9.232	8.790	8.788	9.169	10.120	9.694	9.090	9.968	9.382	9.331	9.924	8.588
SAEAFPLEF	9.358	7.940	6.435	9.640	9.913	9.390	10.064	9.801	10.232	9.615	9.813	8.903	9.248	11.650	9.339	10.860	8.272	9.372	9.596	10.075	10.031	9.878	8.836	8.865	11.018	10.091	9.122	9.572	8.784	8.408	7.955	9.884
SMEHFRWGKPV(-.98)	10.129	10.881	7.581	10.658	10.154	9.251	10.674	7.084	11.233	10.317	9.314	9.289	10.015	8.377	9.963	9.475	8.483	10.066	9.961	7.441	9.134	10.340	8.141	7.806	9.852	8.606	8.946	10.173	8.648	10.367	10.423	8.974
SYSMEHF	9.247	8.959	10.628	9.595	8.941	10.125	8.791	9.971	11.319	10.421	9.747	11.126	8.862	10.113	9.399	9.842	9.514	10.520	8.001	9.419	10.574	11.313	9.868	10.707	11.133	8.301	9.714	9.176	9.298	10.469	10.752	8.903
SYSMEHFRWGKPV(-.98)	10.034	9.171	10.232	8.995	7.377	7.947	9.929	9.575	8.615	9.339	9.078	7.590	9.238	7.863	9.149	9.111	9.433	10.451	8.497	10.192	8.798	8.851	8.841	9.525	10.135	8.089	8.811	9.178	7.823	10.202	10.653	8.794
TPLVTLF	9.350	8.578	6.374	10.409	10.283	8.861	10.273	10.885	10.514	10.728	8.728	8.891	8.950	9.773	11.041	10.410	8.145	10.333	7.686	9.978	10.180	10.661	7.549	9.170	8.097	9.805	8.276	10.037	8.420	10.788	10.717	10.481
TPLVTLFKNAIIKN	9.637	7.612	9.638	9.027	7.682	9.019	9.618	9.349	8.392	9.830	8.519	9.383	8.836	7.118	10.233	9.761	8.067	8.456	8.975	9.760	8.662	8.780	8.324	7.643	10.949	8.221	9.558	8.649	8.978	8.046	8.462	7.941
Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLF	14.631	9.130	8.671	12.748	15.697	14.656	13.897	15.015	14.012	16.272	12.285	15.327	13.763	15.918	14.891	15.069	13.023	12.769	12.512	14.933	15.016	12.981	11.093	14.754	10.266	11.492	12.313	10.370	14.599	12.402	11.483	13.321
Y(+42.01)GGFMTSEK	9.790	7.508	9.223	9.774	7.992	8.143	9.487	9.728	7.568	9.261	10.313	7.617	8.557	9.064	6.490	9.847	7.459	10.480	9.003	9.271	9.152	10.078	7.481	6.843	10.405	7.096	9.074	8.583	8.486	7.171	10.943	8.902
Y(+42.01)GGFMTSEKSQ	9.810	8.728	9.409	10.460	8.508	10.362	10.691	9.180	8.791	9.871	9.011	8.027	8.896	7.871	9.608	10.447	8.493	6.122	9.760	8.490	8.948	9.825	8.345	8.939	10.255	8.571	9.992	9.747	9.511	7.954	7.522	10.089
Y(+42.01)GGFMTSEKSQTP	11.121	9.072	9.012	10.730	10.752	11.061	11.474	11.825	11.778	12.313	11.351	12.048	11.742	11.543	11.777	10.750	10.775	10.609	10.536	11.073	10.860	10.599	9.964	10.925	10.367	9.571	10.840	10.641	10.686	10.072	10.264	10.936
Y(+42.01)GGFMTSEKSQTPL	10.968	10.011	9.804	9.834	10.597	11.395	11.138	12.451	11.292	11.702	10.836	11.592	9.735	10.467	10.877	10.507	10.275	10.290	10.256	11.120	11.114	11.419	9.110	10.060	11.578	10.198	10.009	10.398	11.233	10.566	10.625	11.337
Y(+42.01)GGFMTSEKSQTPLV	10.721	9.855	9.231	11.046	11.383	11.677	11.282	12.231	11.056	11.693	10.277	11.800	10.010	11.488	11.172	10.440	10.381	11.056	10.378	10.920	11.553	11.116	8.885	10.567	10.906	10.287	10.819	10.322	11.119	9.702	10.628	10.918
Y(+42.01)GGFMTSEKSQTPLVT	11.192	10.015	8.280	10.722	10.825	11.385	10.770	12.133	10.904	11.040	10.590	11.464	10.271	11.336	10.899	10.652	10.148	11.068	9.781	10.283	11.218	9.873	9.524	9.806	11.891	9.859	9.883	10.302	10.667	11.060	10.129	11.508
Y(+42.01)GGFMTSEKSQTPLVTL	11.774	10.166	10.819	11.803	13.459	11.729	11.908	13.921	12.425	12.346	11.600	12.041	11.613	12.934	12.356	12.157	12.215	12.252	11.525	12.025	13.207	12.775	11.111	11.658	12.854	12.263	12.084	12.685	12.753	12.484	11.948	13.750
Y(+42.01)GGFMTSEKSQTPLVTLF	14.952	11.903	11.423	14.427	18.713	16.737	15.461	18.018	16.197	17.091	14.769	16.549	15.841	18.102	16.080	16.722	15.124	15.473	14.874	16.765	17.822	17.197	12.747	15.901	12.663	12.795	13.978	14.940	17.166	16.452	14.816	16.920
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIK	12.788	10.713	11.824	11.047	11.588	11.545	11.818	14.238	12.779	12.788	12.955	12.692	13.172	13.190	12.736	12.850	13.394	13.999	13.054	13.596	14.169	11.178	11.614	11.479	14.537	12.260	12.022	11.445	12.722	13.030	12.613	13.458
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKN	12.300	10.915	12.041	11.491	12.906	12.026	11.767	13.890	12.266	12.590	12.272	12.188	12.860	12.723	12.635	13.425	12.642	12.702	12.554	12.747	13.984	12.317	11.869	12.189	14.125	12.243	11.728	11.558	13.004	13.258	12.790	14.327
Y(+42.01)SMEHFRWGKPV(-.98)	11.022	9.564	6.819	9.388	8.851	9.115	11.202	10.380	11.300	9.978	9.944	9.410	9.039	8.891	10.309	9.654	8.183	9.674	9.463	9.532	8.830	9.114	8.341	8.049	9.632	8.965	8.913	10.645	8.576	10.121	10.314	9.448
YGGFMTSEKSQTPLVT	9.369	8.006	10.908	7.052	7.995	8.870	8.484	10.983	11.449	9.974	9.622	9.493	9.296	10.672	9.641	9.452	10.406	10.017	10.759	11.921	9.805	11.150	9.901	9.954	12.218	8.494	10.098	11.053	8.439	10.066	10.042	9.448
YGGFMTSEKSQTPLVTL	10.715	9.784	11.422	9.274	10.020	11.075	9.730	11.249	12.184	11.702	10.761	12.382	11.835	11.019	11.453	10.447	9.555	9.973	11.453	11.259	10.969	10.980	9.742	10.857	11.166	9.280	9.707	9.347	11.916	11.631	12.454	10.758
YGGFMTSEKSQTPLVTLF	10.668	10.855	11.801	11.683	12.426	10.695	11.096	12.196	13.159	13.083	10.706	12.229	11.397	12.952	10.959	12.033	10.689	11.809	11.387	11.762	13.159	12.806	11.116	12.953	12.236	12.327	11.351	11.916	11.770	12.664	12.381	12.403
YGGFMTSEKSQTPLVTLFKNAIIKN	9.692	10.019	10.545	10.545	10.875	9.316	8.709	11.074	10.792	12.263	10.506	10.439	9.593	11.066	9.951	10.430	10.038	12.192	11.409	10.882	11.198	9.082	10.258	11.478	13.592	9.422	10.548	7.892	8.862	12.513	12.496	10.520
LPGLLLF	10.755	10.993	10.041	8.664	12.688	9.624	10.071	11.814	11.975	10.662	10.237	9.270	10.664	13.162	10.501	11.643	9.494	11.310	9.075	11.602	12.976	10.479	11.492	11.685	8.843	10.716	11.923	11.983	11.474	11.736	11.382	11.781
LPHLPGLLLF	10.910	10.862	12.252	8.922	11.820	8.722	11.220	11.036	11.630	11.537	10.003	8.340	10.157	10.041	11.650	12.350	9.728	10.673	10.528	12.336	12.608	10.939	12.021	12.939	9.760	12.380	12.086	9.212	11.681	13.003	10.330	12.659
TPLPELFDRVVML	6.582	5.516	12.778	12.346	9.515	6.239	10.748	10.694	12.665	7.966	7.743	9.159	11.642	12.286	12.060	8.631	10.788	11.244	8.945	10.927	11.150	12.426	10.284	11.804	13.561	13.398	10.206	12.926	11.197	13.640	13.493	10.961
F(+42.01)PAMPLSSLFANAVLRAQHLH	11.815	12.604	11.098	12.085	12.449	11.576	12.865	12.629	11.733	12.533	11.232	11.345	12.827	12.642	9.258	14.088	10.708	12.214	13.864	11.753	10.661	12.519	11.983	11.092	10.577	9.220	12.671	12.393	12.116	13.855	10.648	12.014
FPAM(+15.99)PLSSLFANAVLR	11.431	11.302	12.781	10.435	13.220	11.568	9.189	11.362	10.809	12.480	10.692	11.349	11.903	12.915	11.116	13.102	12.757	11.176	11.269	11.813	13.889	10.914	13.011	13.053	10.961	9.594	12.216	12.827	12.663	10.886	11.152	10.890
FPAM(+15.99)PLSSLFANAVLRAQH	10.285	11.636	10.515	10.230	10.388	11.836	11.678	10.465	10.251	11.194	10.232	9.948	10.753	11.118	9.910	10.632	12.234	9.601	8.944	10.630	12.046	10.270	11.611	12.011	8.724	8.767	11.200	11.349	10.225	10.249	8.149	11.959
FPAM(+15.99)PLSSLFANAVLRAQHLH	10.179	11.230	12.338	8.446	11.580	11.415	9.077	9.605	9.984	11.261	11.028	10.119	10.215	9.502	9.979	9.651	11.508	10.722	10.110	9.605	10.988	10.751	12.114	12.283	10.153	9.688	11.150	9.257	10.949	10.947	8.151	9.220
FPAMPLS	8.678	9.590	8.878	8.984	10.439	10.446	9.062	9.303	8.577	9.358	8.506	9.277	8.844	9.647	8.073	9.549	7.796	7.734	9.415	8.788	9.156	8.828	9.385	9.076	9.167	8.109	9.949	8.978	9.058	9.322	8.486	9.674
FPAMPLSS	9.098	10.865	10.825	9.924	10.167	11.338	9.450	9.533	9.737	8.809	9.802	10.573	9.249	9.901	9.180	9.002	9.054	9.013	9.567	9.282	10.111	9.799	10.025	9.908	9.006	9.179	10.781	9.783	10.461	10.225	9.774	9.897
FPAMPLSSL	9.480	10.912	11.565	10.558	11.624	11.955	8.887	10.707	8.344	10.272	10.186	10.886	10.208	10.118	9.198	10.309	9.577	9.526	9.944	9.552	10.765	10.065	10.462	10.340	10.181	10.090	10.769	10.073	10.660	10.222	10.266	10.433
FPAMPLSSLF	9.320	10.246	10.664	9.741	11.886	11.303	9.025	10.478	9.950	10.553	10.397	10.591	10.463	11.962	9.616	10.702	10.288	9.213	9.661	9.632	11.322	9.680	10.700	11.279	9.566	9.966	11.369	8.296	12.079	10.590	9.103	10.776
FPAMPLSSLFA	10.252	11.975	14.973	11.851	15.091	12.988	9.441	12.320	11.228	11.842	12.328	12.403	12.571	14.208	11.769	11.721	12.733	11.255	10.969	11.605	14.319	11.961	13.748	13.905	12.071	13.081	13.942	11.146	15.020	13.796	11.896	13.655
FPAMPLSSLFAN	10.276	11.635	14.339	11.783	14.655	13.012	9.800	12.274	11.136	11.593	11.695	12.088	12.194	14.044	11.484	11.740	12.751	11.391	11.374	11.710	14.004	11.944	13.778	13.424	11.823	12.422	13.661	10.973	14.318	13.578	11.734	13.559
FPAMPLSSLFANA	10.368	11.652	14.661	11.860	14.825	6.264	12.658	12.541	11.278	13.006	11.771	12.325	12.574	10.067	12.194	12.597	12.849	13.558	11.727	12.018	14.671	8.567	14.428	14.140	12.897	13.384	14.050	10.813	15.159	14.214	12.382	15.136
FPAMPLSSLFANAVLR	10.578	11.804	14.302	11.929	14.552	11.935	10.163	12.379	12.284	11.213	12.276	11.769	12.339	13.881	11.925	12.468	12.723	11.668	11.720	11.555	14.258	12.194	14.058	14.082	12.296	13.384	13.631	11.725	14.686	14.308	11.948	13.990
FPAMPLSSLFANAVLRA	10.856	10.838	9.845	10.384	12.662	11.015	9.723	11.748	9.783	10.114	11.096	10.178	9.802	11.412	8.779	11.252	9.141	8.382	10.955	9.565	10.802	9.255	8.217	11.701	10.042	10.402	11.498	9.550	12.654	11.745	9.127	11.666
FPAMPLSSLFANAVLRAQ	12.352	11.357	10.439	13.333	15.756	12.906	11.159	13.832	13.139	12.552	14.171	14.615	14.467	16.004	14.595	14.839	14.911	14.577	13.822	13.593	13.957	12.126	12.771	14.614	11.444	13.311	13.397	11.292	14.775	13.586	11.695	12.845
FPAMPLSSLFANAVLRAQH	10.058	11.531	11.806	11.422	12.701	11.249	9.500	11.044	8.907	10.026	11.264	9.819	10.358	11.019	10.067	11.074	11.406	11.148	10.667	10.272	11.325	10.482	11.965	11.595	10.452	11.527	11.154	9.869	11.901	12.470	10.266	11.820
FPAMPLSSLFANAVLRAQHLH	10.447	11.869	11.968	11.406	10.458	11.043	10.044	11.177	10.046	9.718	11.844	10.303	10.046	9.609	10.811	11.483	11.959	11.368	10.744	10.142	10.638	11.117	11.876	11.476	10.625	11.118	10.848	9.835	11.518	12.394	10.046	11.407
FPAMPLSSLFANAVLRAQHLHQ	10.607	11.988	11.754	11.501	10.970	11.110	10.610	11.575	11.344	10.258	11.849	10.544	10.740	10.728	11.215	12.070	11.848	11.690	11.141	10.175	11.120	11.367	12.626	12.042	11.403	11.426	11.341	10.310	11.753	12.776	10.503	11.683
FPAMPLSSLFANAVLRAQHLHQL	11.717	12.731	12.777	12.285	10.265	11.131	11.516	13.035	11.760	10.878	13.299	11.936	10.693	9.330	11.537	13.180	12.875	11.846	12.389	11.250	11.312	12.320	11.703	12.217	11.157	11.941	11.581	10.912	12.616	13.863	10.902	13.258
FPAMPLSSLFANAVLRAQHLHQLA	11.949	12.873	12.620	12.366	10.917	11.334	11.320	12.906	12.065	11.060	13.602	12.011	10.910	10.250	11.364	13.200	12.787	12.031	12.236	11.202	11.485	12.402	12.052	12.651	11.812	12.106	11.742	11.188	12.493	14.062	11.148	13.324
FPAMPLSSLFANAVLRAQHLHQLAAD	11.526	11.684	11.763	12.321	11.354	11.053	11.828	13.223	11.809	10.742	12.319	11.232	10.731	10.731	10.478	13.555	12.002	10.737	12.483	10.931	11.297	11.841	11.724	12.972	11.680	11.305	11.632	11.950	12.368	13.781	10.623	12.953
GPVQFLSRIF	9.344	9.443	10.153	9.508	9.171	9.556	10.249	10.143	10.628	10.394	7.751	9.937	9.726	10.892	11.017	7.500	7.972	10.320	8.611	10.892	9.403	9.748	10.407	10.220	9.698	10.791	9.048	10.570	9.861	10.058	10.681	8.606
PVQFLSRIF	8.529	8.273	8.786	8.410	8.948	9.115	9.715	9.980	9.711	8.372	6.711	9.276	9.468	9.715	10.526	9.190	7.681	9.993	7.980	10.203	8.888	9.525	9.628	8.480	9.056	10.330	9.796	7.293	7.801	9.848	10.883	10.289
SPRIGQILK	8.974	8.974	8.025	8.692	9.112	10.296	6.732	8.903	7.275	7.685	5.757	9.301	9.409	9.836	10.227	9.582	8.631	10.059	8.839	8.305	8.887	8.747	9.479	9.494	9.022	7.720	9.969	7.603	8.184	7.631	5.531	10.018
SPRIGQILKQ	8.078	6.759	4.407	6.927	8.931	6.048	7.906	8.367	7.425	7.937	5.605	8.597	6.811	6.902	6.180	8.428	6.134	9.827	7.831	6.503	6.817	6.980	7.209	6.858	7.845	6.065	6.277	6.043	4.997	6.458	9.888	7.758
TDMELLRF	7.105	6.031	6.862	7.937	7.885	7.252	7.535	8.499	8.474	7.715	9.609	7.809	8.828	9.594	10.004	7.350	7.969	9.450	7.893	8.107	7.356	7.384	7.547	5.994	8.435	11.185	8.084	5.261	11.306	8.615	5.601	5.792
ASLDKFLASVSTVLTSKYR	10.389	12.977	11.871	11.104	12.586	11.707	12.117	10.686	11.729	11.510	12.561	10.872	9.651	12.425	11.272	12.982	13.150	11.873	9.276	11.859	11.471	12.912	11.396	11.396	12.765	13.767	11.628	8.890	13.203	10.753	12.106	8.508
FLASVSTVLTSKYR	9.215	12.144	11.169	10.595	9.349	10.805	11.428	10.455	10.853	11.202	11.836	10.796	9.014	10.303	8.038	11.183	11.483	9.427	7.869	9.673	10.842	11.338	10.140	9.349	10.725	10.354	9.984	10.726	10.622	9.403	11.672	9.119
SLDKFLA	7.143	5.923	4.250	6.030	7.406	6.659	5.553	7.239	6.128	6.829	3.122	5.741	6.343	7.720	6.929	7.570	6.254	9.890	7.572	7.502	6.895	6.958	6.074	4.945	9.126	6.099	4.653	5.055	5.049	9.360	7.034	6.790
SLDKFLASVSTVLTSKYR	9.822	11.583	10.329	10.375	9.169	11.699	13.046	9.976	11.667	5.956	13.100	9.578	8.831	7.874	12.714	9.709	12.977	11.347	9.349	10.122	11.762	11.422	10.958	10.372	11.528	11.944	9.586	10.205	11.088	10.942	10.805	11.130
SVSTVLTSKYR	8.237	10.046	10.045	8.468	8.825	10.638	7.374	8.087	9.380	5.967	11.027	9.764	9.407	11.060	7.276	7.959	10.960	8.542	9.073	8.362	10.367	9.629	9.737	9.398	8.885	9.963	8.794	9.370	9.311	8.767	9.399	11.065
TPAMHASLDKFLASVSTVLTSKYR	9.815	12.188	10.657	8.644	12.094	12.154	11.077	11.727	11.435	8.093	12.905	9.158	10.445	12.037	11.057	12.999	14.250	11.129	9.154	8.906	10.661	12.762	12.139	11.057	11.868	12.794	11.248	7.364	13.316	9.324	11.176	10.284
VHLTDAEKAAVN	9.227	9.785	10.442	8.888	9.621	10.721	8.211	9.312	9.426	7.504	11.040	10.806	9.069	10.554	7.783	8.545	10.938	9.557	9.947	9.712	10.107	9.505	9.982	9.671	9.455	10.714	9.366	9.890	9.689	9.843	9.450	9.463
VHLTDAEKAAVNG	9.251	9.155	9.763	8.993	9.364	9.441	8.069	9.392	9.103	10.361	10.318	9.849	7.641	9.250	7.226	8.135	10.536	9.039	9.445	8.861	9.324	9.640	9.229	9.372	8.924	10.157	8.576	9.226	9.003	9.035	8.639	8.780
AAFQKVVAGVASALAHKYH	9.324	10.990	11.165	8.956	10.191	10.190	5.760	9.042	9.306	10.228	10.646	7.711	8.939	10.978	10.697	7.469	10.609	8.375	8.827	9.074	8.456	10.188	9.818	9.020	9.921	10.895	9.782	9.870	10.062	10.113	10.919	9.462
AFQKVVAGVASALAHKYH	9.299	11.226	11.280	6.946	7.918	9.744	10.639	9.453	9.714	10.091	10.596	8.436	9.489	4.840	10.559	10.747	10.731	8.463	8.458	8.564	8.916	10.130	9.858	8.850	9.597	10.349	8.967	8.611	9.173	9.073	9.622	7.209
AQAAFQKVVAGVASALAHKYH	9.672	9.576	9.429	9.135	9.017	10.195	9.586	7.957	9.376	6.374	9.702	8.002	9.920	9.801	9.987	10.232	10.764	10.525	6.465	8.193	8.561	10.582	9.936	8.924	9.916	10.477	9.224	8.977	9.527	9.072	10.049	6.192
ASALAHKYH	7.976	8.639	9.232	9.343	9.301	9.548	9.978	8.803	9.749	10.428	10.099	9.130	10.049	8.877	10.040	8.541	10.612	6.977	8.476	8.703	8.978	8.912	9.503	9.109	8.426	10.116	9.684	10.292	8.966	8.682	8.396	8.524
GVASALAHKYH	8.759	9.396	10.441	9.198	9.679	10.158	7.556	8.935	5.996	7.210	10.496	8.509	9.732	9.558	7.580	8.723	10.667	9.304	8.750	9.318	9.047	8.643	9.505	9.336	8.905	10.636	8.321	9.448	9.230	8.834	8.636	8.558
QAAFQKVVAGVASALAHKYH	9.535	11.255	10.998	9.234	8.078	10.881	10.999	9.033	9.300	10.358	10.534	8.461	9.585	7.405	10.487	7.942	11.139	9.031	7.524	8.336	8.390	9.865	9.699	9.440	9.532	10.481	9.060	7.751	9.576	9.259	9.773	8.100
VHLTDAEKA	8.390	10.293	8.047	8.439	9.326	6.807	8.773	7.536	9.779	6.693	9.374	9.074	6.816	9.694	6.271	8.050	7.241	10.444	7.962	7.740	10.390	7.651	8.868	8.473	6.520	10.428	9.377	8.339	10.373	8.163	8.026	8.522
GPNLVARSKEALA	8.783	9.638	10.905	9.565	8.562	9.956	8.606	9.053	9.022	7.521	10.433	8.275	8.522	9.223	7.736	7.967	10.806	8.213	10.082	7.869	9.031	9.669	9.278	9.642	9.898	10.549	8.879	9.733	9.647	8.765	9.878	9.086
AKVAVLGA	10.404	9.514	7.990	10.008	10.864	9.270	9.355	9.964	7.373	9.095	9.606	10.131	9.806	9.978	7.193	10.047	10.221	10.936	9.224	8.620	8.499	9.785	9.793	10.182	8.563	8.886	8.672	11.021	9.162	8.300	7.055	9.431
SNRVVDLMAYMASKE	9.290	10.199	10.504	11.153	8.696	10.754	5.734	9.529	8.927	8.043	10.758	9.280	9.483	10.699	10.444	9.612	10.901	11.486	10.171	8.610	10.652	10.585	10.367	9.739	9.737	8.430	9.978	9.825	9.796	9.998	8.982	11.020
VKVGVNGF	9.040	9.405	9.857	10.415	9.329	10.147	7.802	9.813	8.586	8.959	9.913	10.642	8.741	9.661	8.509	9.111	9.959	10.857	9.348	8.728	9.679	10.393	9.870	9.433	10.653	8.784	9.292	8.846	9.417	10.188	8.151	10.873
VKVGVNGFGRIG	9.227	10.254	9.448	9.835	8.691	8.966	7.128	9.302	9.389	10.201	10.147	8.848	8.699	9.297	10.052	8.338	9.584	10.946	9.327	8.901	9.484	9.683	9.513	9.419	9.947	8.483	9.300	9.534	8.519	10.116	8.812	10.175
PHFLYPK	10.374	9.111	7.961	10.005	8.579	9.600	10.410	10.202	10.033	8.874	10.198	9.369	9.306	8.638	9.776	9.854	9.658	9.154	10.222	9.163	8.725	9.530	8.501	8.740	10.362	9.807	8.170	10.468	8.673	9.659	10.054	9.643
SQENPNTYSEDLDV	11.483	10.616	10.050	12.010	12.613	11.935	10.158	11.263	11.976	11.171	11.932	13.312	12.395	12.294	12.102	11.517	10.683	10.951	11.766	10.762	11.655	11.080	9.450	12.068	12.745	10.020	12.157	11.799	11.276	11.821	10.941	12.268
YGGFLRKYP	9.811	10.332	7.065	9.245	9.511	9.567	10.319	9.383	11.561	9.243	9.979	10.062	8.153	8.993	10.152	9.251	9.093	9.201	10.766	9.278	8.825	9.391	8.972	8.684	9.316	9.002	8.676	10.121	8.921	9.983	9.937	9.328
YGGFLRKYPK	9.670	9.501	9.527	8.855	8.571	8.775	10.445	9.247	10.917	8.307	9.341	8.477	9.541	7.869	9.835	8.734	9.965	9.129	10.610	8.335	8.645	9.303	9.534	9.760	8.034	9.625	9.343	10.402	9.268	10.024	9.928	8.409
YGGFLRRI	10.039	10.401	10.044	9.478	9.071	9.132	9.998	9.620	9.298	9.630	9.590	9.547	9.277	7.665	9.539	9.275	9.205	9.444	10.499	9.635	8.697	9.633	9.319	9.071	9.097	9.240	8.999	9.961	9.175	9.991	10.483	9.981
YGGFLRRQFKVVT	10.415	11.126	10.274	7.495	10.082	9.522	10.459	9.417	10.562	9.363	9.479	9.379	8.588	6.814	9.700	9.205	9.249	9.307	10.716	9.433	8.992	10.557	9.319	9.320	9.913	9.304	9.351	10.445	9.381	10.357	10.499	9.260
MDQLAKELT	9.702	8.856	8.590	9.833	9.248	8.064	9.648	9.754	10.290	10.032	8.411	9.187	9.385	9.740	10.535	10.345	8.173	10.121	9.294	10.388	9.457	9.305	9.743	10.204	8.415	8.442	9.753	9.809	9.324	10.634	9.137	9.672
MDQLAKELTAE	9.430	10.127	9.460	10.240	9.647	10.058	10.102	9.859	10.683	10.227	9.178	9.679	8.906	9.828	10.424	10.147	10.305	9.380	12.062	10.872	10.118	9.737	9.841	8.736	10.000	9.988	9.887	9.309	9.451	11.010	10.650	10.859
WSRMDQLAKELT	9.254	9.684	8.691	9.921	10.288	8.488	10.740	9.585	10.545	10.747	8.310	9.236	9.935	10.082	11.088	10.391	9.387	10.736	8.559	9.965	9.801	9.202	10.080	10.288	9.727	8.894	9.944	8.829	9.964	10.830	9.988	9.004
WSRMDQLAKELTAE	10.576	10.117	10.259	10.796	10.406	9.845	11.433	10.469	11.401	11.233	9.697	9.565	11.024	10.493	11.709	10.744	7.958	10.939	10.491	11.001	10.945	9.970	10.607	10.566	10.749	10.573	10.524	10.104	10.478	11.600	11.776	9.987
NQEQAEQGREHL	9.888	9.637	7.969	10.143	10.229	7.774	10.162	8.135	10.664	10.212	9.088	8.712	9.694	10.674	10.807	10.837	8.465	10.451	8.603	9.848	11.302	7.242	10.916	11.972	9.383	9.394	10.523	8.738	9.143	9.565	8.422	9.395
Q(-17.03)ELGKLTGPSNQ	9.983	10.190	9.639	9.901	9.840	8.876	10.054	9.310	10.468	10.614	8.110	9.545	9.532	9.754	10.952	10.002	9.218	10.064	9.415	10.454	10.068	9.554	8.860	10.088	9.598	9.409	10.784	9.779	9.554	10.180	9.921	9.569
YEENSRENPF	10.210	9.409	9.681	9.335	9.732	8.421	10.084	10.389	11.745	10.688	9.797	10.822	8.245	8.699	9.843	9.056	8.268	10.241	10.125	9.793	10.218	9.777	9.468	9.856	9.991	8.834	10.266	9.815	9.908	10.889	10.720	10.551
YKVNEYQGPVAPSGGFFLFRPRN(-.98)	10.379	10.759	9.217	10.274	10.310	7.723	10.927	10.017	10.217	9.912	8.458	8.300	8.142	9.116	10.153	10.958	6.720	11.220	9.756	10.189	8.886	9.814	10.730	10.143	10.234	9.706	10.261	9.440	10.129	11.187	9.982	9.495
Q(-17.03)EPGAPAAGM	10.041	10.083	10.883	11.034	10.892	9.865	9.333	10.319	6.634	10.054	9.505	10.203	9.335	9.054	9.997	9.601	8.516	10.517	10.348	11.674	9.603	9.542	9.720	9.541	11.288	9.308	9.341	10.410	10.449	10.126	9.746	10.830
Q(-17.03)EPGAPAAGM(+15.99)	10.671	10.131	10.461	10.420	9.508	9.705	9.950	8.049	10.735	11.634	9.279	10.496	10.652	9.773	10.496	10.320	10.239	9.646	10.167	10.318	9.789	10.645	10.025	10.615	10.019	8.082	9.347	10.271	9.987	10.621	9.834	9.184
A(+42.01)AYKLVLIRH	9.220	10.393	9.878	9.891	8.033	10.339	9.692	9.767	9.283	10.096	9.335	8.548	8.769	8.189	9.977	8.407	9.603	9.881	8.517	8.310	9.500	9.336	9.428	8.144	8.833	9.973	9.944	9.155	9.204	9.245	8.465	8.614
A(+42.01)AYKLVLIRHGE	8.575	9.094	8.363	9.404	8.027	8.630	9.948	8.635	8.528	7.581	8.946	8.001	8.914	7.837	10.531	9.399	9.236	10.652	8.051	9.340	8.880	8.635	9.706	9.359	8.941	7.281	9.171	8.014	7.339	8.487	9.977	8.909
SVNPYLQ(-.98)	9.316	9.929	9.431	8.501	9.298	9.567	9.907	10.660	10.342	9.533	9.691	11.010	9.109	9.094	9.542	8.816	9.104	10.040	11.143	10.273	9.505	9.449	9.661	9.279	10.194	9.102	9.861	10.051	9.696	9.854	8.651	10.334
SVNPYLQGKRLDNVV	9.806	10.633	10.343	10.439	9.478	9.674	10.305	8.825	11.062	10.298	9.161	9.572	9.477	9.756	10.900	9.588	8.584	9.805	9.461	10.445	10.638	9.828	10.404	10.585	8.421	9.424	10.249	9.235	9.918	11.302	10.682	10.520
SVNPYLQGKRLDNVVA	10.065	10.015	10.235	10.252	9.164	9.938	10.248	9.840	10.995	10.097	9.181	10.031	9.497	9.504	10.600	9.558	9.069	9.988	10.329	9.980	10.271	10.081	9.628	9.970	9.989	9.933	9.982	9.899	9.699	10.261	10.925	9.867
SVPHFSEEEKEPE	10.435	8.977	10.152	10.200	8.703	9.006	10.225	8.646	10.746	9.462	9.817	11.170	10.664	10.007	10.327	9.003	10.148	10.148	10.391	11.113	10.218	9.076	9.467	9.624	9.355	9.450	10.179	10.267	9.656	10.507	10.065	10.189
VPHFSEEEKEPE	8.949	9.755	10.748	9.620	8.959	9.459	10.326	9.382	10.628	9.729	10.250	10.143	8.691	9.513	9.687	8.412	10.847	9.756	10.799	11.015	8.802	10.869	10.053	9.738	10.203	9.028	10.171	9.291	9.537	10.725	9.922	10.246
IKMALQQEGFD	10.327	9.935	8.989	13.103	5.635	9.904	9.992	10.784	9.780	9.551	9.799	9.524	8.067	9.794	9.691	9.946	9.405	8.456	9.996	9.824	8.557	9.317	7.672	8.333	9.905	9.569	8.454	10.038	9.807	10.055	9.662	10.468
PMFIVNTNVPRA	9.481	9.944	9.483	10.555	9.247	9.369	6.179	10.439	7.868	7.924	10.364	9.962	9.344	9.218	11.289	11.187	10.799	11.324	8.532	10.137	9.828	10.490	6.809	10.004	9.592	10.258	9.451	10.798	8.578	8.643	10.481	8.817
TAYAIYMLAMAAFPKKQN	9.792	9.406	11.493	10.320	10.771	9.617	9.039	10.464	9.754	10.713	10.137	10.545	10.015	11.646	7.452	11.557	10.504	8.267	10.426	10.208	10.575	10.732	10.753	10.709	11.385	9.482	10.352	11.849	10.172	9.396	11.222	11.059
SAVISLEGKPL	9.283	9.869	9.291	10.359	10.551	9.979	9.846	10.500	7.133	10.369	9.263	8.640	9.559	8.653	9.858	10.120	9.636	10.569	8.604	9.256	9.519	9.339	9.188	9.136	9.838	9.759	9.802	8.342	8.695	8.345	10.111	9.771
FPKPEGSQD	9.654	8.779	9.914	8.708	9.827	9.702	10.265	9.373	10.370	10.448	9.344	9.130	10.228	9.890	10.010	10.465	10.584	7.681	9.477	10.459	9.565	9.617	9.922	9.907	7.459	9.959	10.392	8.890	9.927	10.213	9.499	9.472
FPKPEGSQDKSLHN	9.592	8.801	11.084	10.049	10.067	9.452	9.211	9.262	6.576	8.797	9.462	8.082	11.133	8.716	9.588	10.025	10.738	10.246	9.346	9.741	9.776	9.091	10.460	9.892	8.730	10.256	9.557	9.493	9.640	9.342	9.099	9.414
SLKPEFVDIINAKQ	9.077	10.636	9.445	10.803	9.309	9.707	10.295	11.267	9.044	11.213	9.422	8.791	9.433	9.779	10.141	9.289	10.885	11.479	8.863	8.231	9.530	10.061	10.711	10.404	10.488	8.683	9.593	9.515	10.980	9.407	7.075	9.892
GRTLYGFGG	9.052	9.920	10.032	9.646	9.098	10.417	10.069	9.619	9.561	8.770	10.276	9.455	8.697	9.161	8.266	9.257	9.647	8.008	9.097	8.557	9.280	9.673	9.131	9.580	9.715	9.191	9.742	9.252	9.889	9.229	9.521	9.495
RTLYGFGG	8.993	9.236	10.180	9.835	9.690	10.084	8.359	9.578	8.694	9.629	10.465	9.207	8.581	9.467	8.280	8.330	9.869	7.764	8.541	8.058	9.230	9.574	9.093	9.933	9.996	9.116	9.000	8.950	9.963	9.208	9.572	10.310
TLYGFGG	7.745	8.325	7.751	9.127	9.166	8.564	8.950	9.439	8.993	9.464	9.263	8.478	7.874	8.975	8.284	8.538	8.598	9.068	9.397	8.580	9.263	9.246	8.188	8.461	9.708	8.310	8.462	8.634	8.586	8.817	8.243	9.564
MQIFVKTL	9.379	10.609	9.643	10.331	8.209	10.703	9.397	10.384	9.270	9.995	10.246	9.456	8.145	8.989	8.272	9.575	8.781	8.208	9.522	8.393	9.359	9.689	8.624	9.317	8.943	8.181	9.577	8.985	9.928	8.951	8.676	10.166
LLRALASSNARAQQ	10.354	9.205	7.363	10.050	9.867	9.814	10.518	9.468	10.899	8.767	9.818	10.555	9.604	10.244	10.777	9.981	9.595	9.476	10.328	10.529	9.176	9.238	9.006	9.656	9.852	10.214	8.716	10.093	9.062	10.415	10.148	9.609
AVDQDLGPEVPPENVL(-.98)	10.191	10.630	10.364	10.961	11.557	10.420	10.447	11.239	11.178	11.865	9.808	10.830	10.599	11.273	11.504	11.311	8.906	10.232	10.564	11.144	11.528	10.372	9.851	11.320	10.775	9.887	11.536	10.431	11.240	11.429	10.841	11.307
LENSSPQAPA	9.115	9.447	8.900	8.364	10.099	9.508	9.884	9.022	10.681	9.519	8.722	10.222	10.120	9.965	10.939	9.870	10.099	9.683	10.315	10.974	10.091	9.933	9.989	10.328	7.969	9.925	9.785	9.600	9.287	9.659	8.710	9.670
LENSSPQAPARRLLPP	9.925	10.410	9.632	7.370	9.526	8.733	9.912	9.415	11.461	9.732	9.054	9.678	7.942	9.083	10.590	9.212	9.005	9.556	9.727	11.540	9.812	9.584	10.048	10.076	9.574	9.656	9.828	9.491	9.040	10.461	10.478	9.630
SLSAASAPLAETSTPL	10.116	10.138	10.111	10.044	10.696	10.363	10.242	10.498	10.845	10.426	9.597	11.202	10.197	10.455	11.410	10.261	9.498	10.337	10.317	11.138	10.607	10.169	10.059	10.455	10.544	9.901	10.491	9.999	10.596	10.888	10.806	10.581
SLSAASAPLAETSTPLRL	10.764	10.153	9.825	9.518	10.885	10.351	10.018	10.858	11.520	11.399	9.349	11.506	9.557	9.433	11.059	9.954	9.811	10.124	10.658	9.704	10.572	10.129	10.022	9.671	11.024	9.907	10.099	10.255	10.527	10.682	11.097	10.351
SSEPEAAPAPRRL	9.898	9.141	8.506	8.878	10.266	8.494	10.181	9.843	9.421	8.893	9.145	8.740	9.860	9.658	9.716	9.958	9.581	9.679	9.054	9.896	9.359	8.275	10.350	10.357	8.308	9.462	9.900	9.708	9.220	10.293	8.972	9.980

Peak intensity plots

EigenMS removes sample-to-sample variability.

Intensity after log2 transformation

Intensity after EigenMS normalization

Univariate analysis

Univariate statistic analysis will proceed for each pairs of comparison. For each pair,

Step 1, differential analysis;

• t-test

• Wilcoxon rank-sum test

For all statistical tests, the Benjamini-Hochberg (BH) procedure was applied to correct for multiple hypothesis testing.

Step 2, fold change, both linear and log2;

Step 3, regulation shown in volcano plot.

• A feature is considered unregulated when FC(log2) > 0.6 (or FC(lin) > 1.5);

• A feature is considered downregulated when FC(log2) < -0.6 (or FC(lin) < -1.5).

Code

d5 <- rbind.data.frame(labels_d1, norm_d1_mod) 
d5_mod <- t(d5) %>%
  as.data.frame(.) %>%
  rownames_to_column(., var = "rowname") %>%
  as_tibble() %>%
  dplyr::rename(., Label = class) %>%
  mutate(across(-c(Label,rowname), as.numeric)) %>%
  #rename_with(str_trim)
  column_to_rownames(., var = "rowname")

#colnames(d5_mod)[-1] == feature_name$variable
colnames(d5_mod)[-1] = feature_name$name

grps = as.factor(t(labels_d1))
trt= levels(grps)

combo_list = do.call("c", lapply(seq_along(time), function(i) combn(trt, 2, FUN = list))) # top 10 are unique combo
#combo_list = combo_list[1:10]

feature_meta = read.csv(file = "feature_meta.csv")

Iso vs No

Full stat

Code

contrast = combo_list[[1]]

df = d5_mod %>%
    dplyr::filter(., Label %in% contrast ) %>%
    droplevels()

temp_d <- df %>%
        mutate(across(-Label, ~ 2^(.) )) %>% # d3_mod is log-scale number, this step will de-log and change data back to linear number
        group_by(Label) %>%
        summarise(across(everything(), mean))

print(paste("Order of Fold Change:", temp_d$Label[1], "over", temp_d$Label[2], sep = " "))

[1] "Order of Fold Change: Iso over No"

Code

uni_res <- do_univariate(df, order = c("No", "Iso"))

uni_res <- uni_res %>%
        rowwise() %>%
        mutate(padj = min(c(BHT, BHW))) %>%
        # get the lowest padj
        ungroup() %>%
        mutate(`-log10padj` = -log10(padj))

uni_res_annotation = uni_res %>% left_join(., feature_meta, by = c("variable" = "Peptide"))

uni_res_annotation %>% 
        knitr::kable(., digits = 3, "html") %>%
        kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
        kableExtra::scroll_box(width = "100%", height = "500px")

variable	pT	BHT	pW	BHW	FC(lin)	FC(log2)	padj	-log10padj	Accession	org_peptide_name
A(+42.01)AYKLVLIRH	0.697	0.853	0.809	0.897	1.087	0.121	0.853	0.069	P25113|PGAM1_RAT	A(+42.01)AYKLVLIRH
A(+42.01)AYKLVLIRHGE	0.703	0.853	1.000	1.000	1.262	0.335	0.853	0.069	P25113|PGAM1_RAT	A(+42.01)AYKLVLIRHGE
AAFQKVVAGVASALAHKYH	0.520	0.714	0.756	0.874	0.905	-0.143	0.714	0.146	P02091|HBB1_RAT:P11517|HBB2_RAT	AAFQKVVAGVASALAHKYH
AEAFPLEF	0.386	0.621	0.468	0.704	1.307	0.386	0.621	0.207	P01194|COLI_RAT	AEAFPLEF
AEEETAGGDGRPEPSP	0.982	0.982	0.705	0.869	1.144	0.194	0.869	0.061	P01194|COLI_RAT	AEEETAGGDGRPEPSP
AEEETAGGDGRPEPSPR	0.496	0.695	0.512	0.730	0.868	-0.204	0.695	0.158	P01194|COLI_RAT	AEEETAGGDGRPEPSPR
AEEETAGGDGRPEPSPRE(-.98)	0.589	0.767	0.512	0.730	0.965	-0.051	0.730	0.137	P01194|COLI_RAT	AEEETAGGDGRPEPSPRE(-.98)
AFQKVVAGVASALAHKYH	0.757	0.868	0.918	0.959	0.967	-0.048	0.868	0.062	P02091|HBB1_RAT:P11517|HBB2_RAT	AFQKVVAGVASALAHKYH
AGTQESVDSAKP	0.476	0.680	0.152	0.361	1.397	0.483	0.361	0.443	P01186|NEU2_RAT	AGTQESVDSAKP
AGTQESVDSAKPRVY	0.562	0.744	0.756	0.874	0.976	-0.035	0.744	0.129	P01186|NEU2_RAT	AGTQESVDSAKPRVY
AKVAVLGA	0.224	0.466	0.349	0.603	0.817	-0.291	0.466	0.331	P04636|MDHM_RAT	AKVAVLGA
AQAAFQKVVAGVASALAHKYH	0.714	0.860	0.973	0.985	0.877	-0.189	0.860	0.066	P02091|HBB1_RAT:P11517|HBB2_RAT	AQAAFQKVVAGVASALAHKYH
ASALAHKYH	0.523	0.714	0.756	0.874	1.203	0.267	0.714	0.146	P02091|HBB1_RAT:P11517|HBB2_RAT	ASALAHKYH
ASLDKFLASVSTVLTSKYR	0.165	0.370	0.173	0.384	0.500	-0.999	0.370	0.432	P01946|HBA_RAT	ASLDKFLASVSTVLTSKYR
AVDQDLGPEVPPENVL(-.98)	0.775	0.869	0.197	0.420	0.881	-0.182	0.420	0.377	Q9QXU9|PCS1N_RAT	AVDQDLGPEVPPENVL(-.98)
DEGHDPVHESPVDTA	0.040	0.171	0.061	0.208	1.611	0.688	0.171	0.767	O35314|SCG1_RAT	DEGHDPVHESPVDTA
DPVHESPVDT	0.849	0.914	0.756	0.874	0.929	-0.106	0.874	0.059	O35314|SCG1_RAT	DPVHESPVDT
DPVHESPVDTA	0.531	0.714	0.605	0.806	1.107	0.146	0.714	0.146	O35314|SCG1_RAT	DPVHESPVDTA
EAFPLEF	0.687	0.847	0.605	0.806	0.971	-0.042	0.806	0.093	P01194|COLI_RAT	EAFPLEF
EEETAGGDGRPEPSPRE(-.98)	0.256	0.507	0.132	0.355	0.888	-0.171	0.355	0.450	P01194|COLI_RAT	EEETAGGDGRPEPSPRE(-.98)
EETAGGDGRPEPSPRE(-.98)	0.074	0.249	0.036	0.150	0.475	-1.075	0.150	0.824	P01194|COLI_RAT	EETAGGDGRPEPSPRE(-.98)
EGFFRLT	0.083	0.259	0.061	0.208	0.734	-0.446	0.208	0.681	P01186|NEU2_RAT	EGFFRLT
ELDQLLHY	0.875	0.921	0.809	0.897	0.971	-0.042	0.897	0.047	O35314|SCG1_RAT	ELDQLLHY
ELEGEQPD	0.746	0.867	0.863	0.931	1.039	0.055	0.867	0.062	P01194|COLI_RAT	ELEGEQPD
ESVDSAKPRVY	0.087	0.259	0.085	0.262	0.590	-0.762	0.259	0.587	P01186|NEU2_RAT	ESVDSAKPRVY
F(+42.01)PAMPLSSLFANAVLRAQHLH	0.751	0.867	0.973	0.985	0.843	-0.246	0.867	0.062	P01244|SOMA_RAT	F(+42.01)PAMPLSSLFANAVLRAQHLH
FLASVSTVLTSKYR	0.542	0.723	0.705	0.869	0.905	-0.144	0.723	0.141	P01946|HBA_RAT	FLASVSTVLTSKYR
FMTSEKSQTP	0.532	0.714	0.468	0.704	1.257	0.330	0.704	0.152	P01194|COLI_RAT	FMTSEKSQTP
FNPYFDPL	0.876	0.921	0.654	0.838	0.896	-0.159	0.838	0.077	O35314|SCG1_RAT	FNPYFDPL
FPAM(+15.99)PLSSLFANAVLR	0.084	0.259	0.173	0.384	0.503	-0.992	0.259	0.587	P01244|SOMA_RAT	FPAM(+15.99)PLSSLFANAVLR
FPAM(+15.99)PLSSLFANAVLRAQH	0.086	0.259	0.173	0.384	0.606	-0.723	0.259	0.587	P01244|SOMA_RAT	FPAM(+15.99)PLSSLFANAVLRAQH
FPAM(+15.99)PLSSLFANAVLRAQHLH	0.025	0.127	0.051	0.196	0.483	-1.051	0.127	0.897	P01244|SOMA_RAT	FPAM(+15.99)PLSSLFANAVLRAQHLH
FPAMPLS	0.225	0.466	0.152	0.361	0.749	-0.417	0.361	0.443	P01244|SOMA_RAT	FPAMPLS
FPAMPLSS	0.148	0.344	0.114	0.324	0.808	-0.308	0.324	0.490	P01244|SOMA_RAT	FPAMPLSS
FPAMPLSSL	0.015	0.088	0.010	0.072	0.623	-0.683	0.072	1.143	P01244|SOMA_RAT	FPAMPLSSL
FPAMPLSSLF	0.000	0.006	0.001	0.011	0.405	-1.305	0.006	2.207	P01244|SOMA_RAT	FPAMPLSSLF
FPAMPLSSLFA	0.000	0.006	0.001	0.011	0.205	-2.286	0.006	2.207	P01244|SOMA_RAT	FPAMPLSSLFA
FPAMPLSSLFAN	0.000	0.006	0.000	0.006	0.240	-2.058	0.006	2.253	P01244|SOMA_RAT	FPAMPLSSLFAN
FPAMPLSSLFANA	0.001	0.020	0.002	0.026	0.238	-2.073	0.020	1.694	P01244|SOMA_RAT	FPAMPLSSLFANA
FPAMPLSSLFANAVLR	0.000	0.003	0.000	0.002	0.225	-2.151	0.002	2.731	P01244|SOMA_RAT	FPAMPLSSLFANAVLR
FPAMPLSSLFANAVLRA	0.001	0.014	0.001	0.016	0.306	-1.708	0.014	1.852	P01244|SOMA_RAT	FPAMPLSSLFANAVLRA
FPAMPLSSLFANAVLRAQ	0.013	0.083	0.016	0.100	0.489	-1.031	0.083	1.080	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQ
FPAMPLSSLFANAVLRAQH	0.000	0.000	0.000	0.001	0.303	-1.722	0.000	3.817	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQH
FPAMPLSSLFANAVLRAQHLH	0.000	0.002	0.000	0.004	0.406	-1.299	0.002	2.652	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLH
FPAMPLSSLFANAVLRAQHLHQ	0.000	0.002	0.000	0.006	0.459	-1.122	0.002	2.652	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQ
FPAMPLSSLFANAVLRAQHLHQL	0.008	0.070	0.008	0.059	0.387	-1.370	0.059	1.227	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQL
FPAMPLSSLFANAVLRAQHLHQLA	0.002	0.030	0.003	0.030	0.375	-1.414	0.030	1.525	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQLA
FPAMPLSSLFANAVLRAQHLHQLAAD	0.001	0.023	0.002	0.026	0.391	-1.356	0.023	1.646	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQLAAD
FPKPEGSQD	0.962	0.982	0.918	0.959	1.012	0.018	0.959	0.018	P47868|SCG3_RAT	FPKPEGSQD
FPKPEGSQDKSLHN	0.120	0.323	0.072	0.232	0.796	-0.329	0.232	0.635	P47868|SCG3_RAT	FPKPEGSQDKSLHN
GPNLVARSKEALA	0.149	0.344	0.152	0.361	0.677	-0.564	0.344	0.464	P02770|ALBU_RAT	GPNLVARSKEALA
GPVQFLSRIF	0.012	0.081	0.008	0.059	1.946	0.960	0.059	1.227	P01244|SOMA_RAT	GPVQFLSRIF
GPYRVEHF	0.067	0.245	0.043	0.168	1.394	0.479	0.168	0.775	P01194|COLI_RAT	GPYRVEHF
GPYRVEHFRWGNPPKD	0.334	0.589	0.314	0.560	1.536	0.619	0.560	0.252	P01194|COLI_RAT	GPYRVEHFRWGNPPKD
GRGREPGAYPALDSRQE	0.600	0.774	0.654	0.838	1.156	0.209	0.774	0.111	O35314|SCG1_RAT	GRGREPGAYPALDSRQE
GRTLYGFGG	0.044	0.186	0.036	0.150	0.772	-0.374	0.150	0.824	P62804|H4_RAT	GRTLYGFGG
GTQESVDSAKPRVY	0.271	0.517	0.349	0.603	0.899	-0.154	0.517	0.286	P01186|NEU2_RAT	GTQESVDSAKPRVY
GVASALAHKYH	0.021	0.117	0.043	0.168	0.540	-0.888	0.117	0.933	P02091|HBB1_RAT:P11517|HBB2_RAT	GVASALAHKYH
IKMALQQEGFD	0.445	0.669	0.973	0.985	1.028	0.040	0.669	0.175	P28841|NEC2_RAT	IKMALQQEGFD
KYVMGHF	0.782	0.869	0.756	0.874	1.079	0.110	0.869	0.061	P01194|COLI_RAT	KYVMGHF
KYVMGHFRWD	0.202	0.437	0.282	0.531	1.391	0.476	0.437	0.360	P01194|COLI_RAT	KYVMGHFRWD
LDEGHDPV	0.009	0.071	0.020	0.104	1.532	0.615	0.071	1.147	O35314|SCG1_RAT	LDEGHDPV
LDEGHDPVHESPV	0.002	0.023	0.000	0.007	2.336	1.224	0.007	2.156	O35314|SCG1_RAT	LDEGHDPVHESPV
LENSSPQAPA	0.256	0.507	0.282	0.531	1.320	0.401	0.507	0.295	Q9QXU9|PCS1N_RAT	LENSSPQAPA
LENSSPQAPARRLLPP	0.142	0.340	0.061	0.208	1.660	0.731	0.208	0.681	Q9QXU9|PCS1N_RAT	LENSSPQAPARRLLPP
LLDEGHDPVHESPVD	0.157	0.358	0.020	0.104	1.472	0.558	0.104	0.983	O35314|SCG1_RAT	LLDEGHDPVHESPVD
LLDEGHDPVHESPVDT	0.479	0.680	0.314	0.560	1.220	0.287	0.560	0.252	O35314|SCG1_RAT	LLDEGHDPVHESPVDT
LLDEGHDPVHESPVDTA	0.002	0.025	0.001	0.016	1.855	0.891	0.016	1.785	O35314|SCG1_RAT	LLDEGHDPVHESPVDTA
LLRALASSNARAQQ	0.019	0.106	0.013	0.083	1.532	0.616	0.083	1.079	Q792G6|GNAS3_RAT	LLRALASSNARAQQ
LPGLLLF	0.074	0.249	0.085	0.262	0.561	-0.833	0.249	0.604	P01230|LSHB_RAT	LPGLLLF
LPHLPGLLLF	0.051	0.206	0.036	0.150	0.479	-1.061	0.150	0.824	P01230|LSHB_RAT	LPHLPGLLLF
LVQLAGTQESVDSAKPRVY	0.390	0.621	0.387	0.634	1.442	0.528	0.621	0.207	P01186|NEU2_RAT	LVQLAGTQESVDSAKPRVY
MDQLAKELT	0.419	0.648	0.654	0.838	1.102	0.140	0.648	0.188	P10354|CMGA_RAT	MDQLAKELT
MDQLAKELTAE	0.428	0.655	0.557	0.781	1.313	0.393	0.655	0.183	P10354|CMGA_RAT	MDQLAKELTAE
MQIFVKTL	0.137	0.340	0.197	0.420	0.715	-0.485	0.340	0.468	P62982|RS27A_RAT:P62986|RL40_RAT:P0CG51|UBB_RAT:Q63429|UBC_RAT	MQIFVKTL
NPYFDPLQWKNSDFE	0.317	0.578	0.223	0.463	0.751	-0.413	0.463	0.334	O35314|SCG1_RAT	NPYFDPLQWKNSDFE
NQEQAEQGREHL	0.678	0.842	0.809	0.897	0.715	-0.484	0.842	0.075	P10362|SCG2_RAT	NQEQAEQGREHL
PESAFSE	0.134	0.340	0.114	0.324	1.317	0.397	0.324	0.490	P01179|NEU1_RAT	PESAFSE
PHFLYPK	0.123	0.325	0.099	0.294	1.317	0.397	0.294	0.532	P05371|CLUS_RAT	PHFLYPK
PMFIVNTNVPRA	0.354	0.605	0.512	0.730	0.868	-0.204	0.605	0.219	P30904|MIF_RAT	PMFIVNTNVPRA
PVHESPVDTA	0.009	0.073	0.006	0.059	1.587	0.666	0.059	1.227	O35314|SCG1_RAT	PVHESPVDTA
PVQFLSRIF	0.335	0.589	0.387	0.634	1.399	0.485	0.589	0.230	P01244|SOMA_RAT	PVQFLSRIF
Q(-17.03)ELGKLTGPSNQ	0.073	0.249	0.223	0.463	1.368	0.452	0.249	0.604	P10362|SCG2_RAT	Q(-17.03)ELGKLTGPSNQ
Q(-17.03)EPGAPAAGM	0.750	0.867	0.863	0.931	1.042	0.059	0.867	0.062	P15087|CBPE_RAT	Q(-17.03)EPGAPAAGM
Q(-17.03)EPGAPAAGM(+15.99)	0.026	0.128	0.029	0.142	1.549	0.632	0.128	0.892	P15087|CBPE_RAT	Q(-17.03)EPGAPAAGM(+15.99)
Q(-17.03)KIAEKFSQ	0.101	0.292	0.114	0.324	1.524	0.608	0.292	0.535	O35314|SCG1_RAT	Q(-17.03)KIAEKFSQ
Q(-17.03)KIAEKFSQR(-.98)	0.011	0.074	0.008	0.059	1.510	0.595	0.059	1.227	O35314|SCG1_RAT	Q(-17.03)KIAEKFSQR(-.98)
QAAFQKVVAGVASALAHKYH	0.785	0.869	0.705	0.869	1.083	0.115	0.869	0.061	P02091|HBB1_RAT:P11517|HBB2_RAT	QAAFQKVVAGVASALAHKYH
R(+42.01)PVKVYPNVAENESAEAFPLEF	0.291	0.542	0.282	0.531	1.400	0.486	0.531	0.275	P01194|COLI_RAT	R(+42.01)PVKVYPNVAENESAEAFPLEF
RAEEETAGGDGRPEPSPRE(-.98)	0.851	0.914	0.918	0.959	1.235	0.304	0.914	0.039	P01194|COLI_RAT	RAEEETAGGDGRPEPSPRE(-.98)
RPVKVYPNVAENE	0.224	0.466	0.282	0.531	1.518	0.602	0.466	0.331	P01194|COLI_RAT	RPVKVYPNVAENE
RPVKVYPNVAENES(+79.97)AEAFPLE	0.460	0.676	0.512	0.730	0.935	-0.097	0.676	0.170	P01194|COLI_RAT	RPVKVYPNVAENES(+79.97)AEAFPLE
RPVKVYPNVAENES(+79.97)AEAFPLEF	0.025	0.127	0.043	0.168	0.487	-1.039	0.127	0.897	P01194|COLI_RAT	RPVKVYPNVAENES(+79.97)AEAFPLEF
RPVKVYPNVAENESAEAFPLE	0.135	0.340	0.314	0.560	1.375	0.459	0.340	0.468	P01194|COLI_RAT	RPVKVYPNVAENESAEAFPLE
RPVKVYPNVAENESAEAFPLEF	0.723	0.860	0.426	0.679	0.918	-0.124	0.679	0.168	P01194|COLI_RAT	RPVKVYPNVAENESAEAFPLEF
RRPVKVYPNVAENESAEAFPLEF	0.661	0.828	0.809	0.897	1.030	0.043	0.828	0.082	P01194|COLI_RAT	RRPVKVYPNVAENESAEAFPLEF
RTLYGFGG	0.003	0.036	0.003	0.030	0.546	-0.873	0.030	1.520	P62804|H4_RAT	RTLYGFGG
S(+42.01)MEHFRWGKPV(-.98)	0.374	0.618	0.512	0.730	1.461	0.547	0.618	0.209	P01194|COLI_RAT	S(+42.01)MEHFRWGKPV(-.98)
S(+42.01)YSMEHF	0.299	0.552	0.282	0.531	0.881	-0.182	0.531	0.275	P01194|COLI_RAT	S(+42.01)YSMEHF
S(+42.01)YSMEHFRWGKPV(-.98)	0.652	0.828	0.705	0.869	0.941	-0.088	0.828	0.082	P01194|COLI_RAT	S(+42.01)YSMEHFRWGKPV(-.98)
SAEAFPLEF	0.970	0.982	1.000	1.000	0.926	-0.111	0.982	0.008	P01194|COLI_RAT	SAEAFPLEF
SAVISLEGKPL	0.321	0.579	0.426	0.679	0.816	-0.294	0.579	0.238	P45592|COF1_RAT	SAVISLEGKPL
SEES(+79.97)QEKEY	0.936	0.971	0.918	0.959	0.980	-0.030	0.959	0.018	O35314|SCG1_RAT	SEES(+79.97)QEKEY
SLDKFLA	0.913	0.954	0.973	0.985	0.684	-0.547	0.954	0.021	P01946|HBA_RAT	SLDKFLA
SLDKFLASVSTVLTSKYR	0.263	0.507	0.251	0.509	0.757	-0.402	0.507	0.295	P01946|HBA_RAT	SLDKFLASVSTVLTSKYR
SLKPEFVDIINAKQ	0.069	0.247	0.072	0.232	0.649	-0.624	0.232	0.635	P48500|TPIS_RAT	SLKPEFVDIINAKQ
SLSAASAPLAETSTPL	0.260	0.507	0.654	0.838	1.197	0.260	0.507	0.295	Q9QXU9|PCS1N_RAT	SLSAASAPLAETSTPL
SLSAASAPLAETSTPLRL	0.142	0.340	0.152	0.361	1.398	0.484	0.340	0.468	Q9QXU9|PCS1N_RAT	SLSAASAPLAETSTPLRL
SMEHFRWGKPV(-.98)	0.028	0.130	0.024	0.124	1.941	0.957	0.124	0.907	P01194|COLI_RAT	SMEHFRWGKPV(-.98)
SNRVVDLMAYMASKE	0.028	0.130	0.020	0.104	0.538	-0.895	0.104	0.983	P04797|G3P_RAT	SNRVVDLMAYMASKE
SPRIGQILK	0.436	0.662	0.387	0.634	0.822	-0.283	0.634	0.198	P01244|SOMA_RAT	SPRIGQILK
SPRIGQILKQ	0.338	0.589	0.468	0.704	1.394	0.479	0.589	0.230	P01244|SOMA_RAT	SPRIGQILKQ
SQENPNTYSEDLDV	0.789	0.869	0.756	0.874	1.044	0.062	0.869	0.061	P06300|PDYN_RAT	SQENPNTYSEDLDV
SSEPEAAPAPRRL	0.143	0.340	0.132	0.355	0.811	-0.303	0.340	0.468	Q9QXU9|PCS1N_RAT	SSEPEAAPAPRRL
SVDSAKPRVY	0.083	0.259	0.132	0.355	0.605	-0.725	0.259	0.587	P01186|NEU2_RAT	SVDSAKPRVY
SVNPYLQ(-.98)	0.359	0.607	0.349	0.603	1.284	0.360	0.603	0.220	P27682|7B2_RAT	SVNPYLQ(-.98)
SVNPYLQGKRLDNVV	0.466	0.676	0.605	0.806	1.100	0.137	0.676	0.170	P27682|7B2_RAT	SVNPYLQGKRLDNVV
SVNPYLQGKRLDNVVA	0.010	0.074	0.013	0.083	1.475	0.560	0.074	1.130	P27682|7B2_RAT	SVNPYLQGKRLDNVVA
SVPHFSEEEKEPE	0.007	0.064	0.005	0.049	1.665	0.736	0.049	1.309	P27682|7B2_RAT	SVPHFSEEEKEPE
SVSTVLTSKYR	0.056	0.215	0.197	0.420	0.452	-1.145	0.215	0.667	P01946|HBA_RAT	SVSTVLTSKYR
SYSMEHF	0.769	0.869	0.605	0.806	1.084	0.116	0.806	0.093	P01194|COLI_RAT	SYSMEHF
SYSMEHFRWGKPV(-.98)	0.377	0.618	0.468	0.704	1.290	0.367	0.618	0.209	P01194|COLI_RAT	SYSMEHFRWGKPV(-.98)
TAYAIYMLAMAAFPKKQN	0.279	0.526	0.314	0.560	0.874	-0.194	0.526	0.279	P35171|CX7A2_RAT	TAYAIYMLAMAAFPKKQN
TDMELLRF	0.579	0.759	0.654	0.838	0.581	-0.784	0.759	0.120	P01244|SOMA_RAT	TDMELLRF
TLYGFGG	0.104	0.295	0.152	0.361	0.801	-0.320	0.295	0.530	P62804|H4_RAT	TLYGFGG
TPAMHASLDKFLASVSTVLTSKYR	0.004	0.045	0.008	0.059	0.212	-2.240	0.045	1.346	P01946|HBA_RAT	TPAMHASLDKFLASVSTVLTSKYR
TPLPELFDRVVML	0.968	0.982	0.863	0.931	1.257	0.331	0.931	0.031	P01237|PRL_RAT	TPLPELFDRVVML
TPLVTLF	0.852	0.914	0.918	0.959	1.047	0.067	0.914	0.039	P01194|COLI_RAT	TPLVTLF
TPLVTLFKNAIIKN	0.015	0.091	0.029	0.142	1.663	0.734	0.091	1.042	P01194|COLI_RAT	TPLVTLFKNAIIKN
TQESVDSAKPRVY	0.354	0.605	0.605	0.806	0.870	-0.201	0.605	0.219	P01186|NEU2_RAT	TQESVDSAKPRVY
VHLTDAEKA	0.125	0.327	0.152	0.361	0.575	-0.799	0.327	0.486	P02091|HBB1_RAT:P11517|HBB2_RAT	VHLTDAEKA
VHLTDAEKAAVN	0.110	0.303	0.152	0.361	0.692	-0.531	0.303	0.519	P02091|HBB1_RAT	VHLTDAEKAAVN
VHLTDAEKAAVNG	0.227	0.466	0.314	0.560	0.785	-0.350	0.466	0.331	P02091|HBB1_RAT	VHLTDAEKAAVNG
VKVGVNGF	0.003	0.036	0.001	0.016	0.572	-0.807	0.016	1.785	P04797|G3P_RAT	VKVGVNGF
VKVGVNGFGRIG	0.409	0.640	0.557	0.781	0.851	-0.233	0.640	0.193	P04797|G3P_RAT	VKVGVNGFGRIG
VPHFSEEEKEPE	0.503	0.699	0.605	0.806	1.149	0.200	0.699	0.155	P27682|7B2_RAT	VPHFSEEEKEPE
VQLAGTQESVDSAKP	0.111	0.303	0.099	0.294	1.520	0.604	0.294	0.532	P01186|NEU2_RAT	VQLAGTQESVDSAKP
VQLAGTQESVDSAKPR	0.627	0.803	0.173	0.384	1.450	0.536	0.384	0.415	P01186|NEU2_RAT	VQLAGTQESVDSAKPR
VQLAGTQESVDSAKPRV	0.464	0.676	0.468	0.704	1.619	0.695	0.676	0.170	P01186|NEU2_RAT	VQLAGTQESVDSAKPRV
VQLAGTQESVDSAKPRVY	0.977	0.982	0.809	0.897	1.096	0.132	0.897	0.047	P01186|NEU2_RAT	VQLAGTQESVDSAKPRVY
WSRMDQLAKELT	0.746	0.867	0.756	0.874	1.123	0.168	0.867	0.062	P10354|CMGA_RAT	WSRMDQLAKELT
WSRMDQLAKELTAE	0.096	0.282	0.061	0.208	1.475	0.561	0.208	0.681	P10354|CMGA_RAT	WSRMDQLAKELTAE
Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLF	0.662	0.828	0.756	0.874	1.017	0.024	0.828	0.082	P01194|COLI_RAT	Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLF
Y(+42.01)GGFMTSEK	0.723	0.860	0.705	0.869	1.160	0.214	0.860	0.066	P01194|COLI_RAT	Y(+42.01)GGFMTSEK
Y(+42.01)GGFMTSEKSQ	0.803	0.878	0.863	0.931	1.119	0.163	0.878	0.057	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQ
Y(+42.01)GGFMTSEKSQTP	0.085	0.259	0.173	0.384	1.428	0.514	0.259	0.587	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTP
Y(+42.01)GGFMTSEKSQTPL	0.956	0.982	0.756	0.874	0.944	-0.083	0.874	0.059	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPL
Y(+42.01)GGFMTSEKSQTPLV	0.863	0.919	0.809	0.897	0.983	-0.025	0.897	0.047	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLV
Y(+42.01)GGFMTSEKSQTPLVT	0.410	0.640	0.512	0.730	0.823	-0.282	0.640	0.193	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVT
Y(+42.01)GGFMTSEKSQTPLVTL	0.039	0.171	0.061	0.208	0.561	-0.835	0.171	0.767	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVTL
Y(+42.01)GGFMTSEKSQTPLVTLF	0.053	0.207	0.072	0.232	0.429	-1.222	0.207	0.684	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVTLF
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIK	0.383	0.621	0.251	0.509	0.732	-0.450	0.509	0.294	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIK
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKN	0.008	0.071	0.008	0.059	0.531	-0.914	0.059	1.227	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKN
Y(+42.01)SMEHFRWGKPV(-.98)	0.011	0.074	0.020	0.104	1.977	0.983	0.074	1.130	P01194|COLI_RAT	Y(+42.01)SMEHFRWGKPV(-.98)
YEENSRENPF	0.373	0.618	0.468	0.704	1.309	0.389	0.618	0.209	P10362|SCG2_RAT	YEENSRENPF
YGGFLRKYP	0.046	0.189	0.036	0.150	1.765	0.820	0.150	0.824	P06300|PDYN_RAT	YGGFLRKYP
YGGFLRKYPK	0.172	0.381	0.282	0.531	1.536	0.619	0.381	0.419	P06300|PDYN_RAT	YGGFLRKYPK
YGGFLRRI	0.027	0.128	0.020	0.104	1.361	0.445	0.104	0.983	P06300|PDYN_RAT	YGGFLRRI
YGGFLRRQFKVVT	0.067	0.245	0.036	0.150	1.476	0.561	0.150	0.824	P06300|PDYN_RAT	YGGFLRRQFKVVT
YGGFMTSEKSQTPLVT	0.232	0.469	0.387	0.634	1.569	0.650	0.469	0.329	P01194|COLI_RAT	YGGFMTSEKSQTPLVT
YGGFMTSEKSQTPLVTL	0.202	0.437	0.152	0.361	1.539	0.622	0.361	0.443	P01194|COLI_RAT	YGGFMTSEKSQTPLVTL
YGGFMTSEKSQTPLVTLF	0.456	0.676	0.387	0.634	0.842	-0.248	0.634	0.198	P01194|COLI_RAT	YGGFMTSEKSQTPLVTLF
YGGFMTSEKSQTPLVTLFKNAIIKN	0.481	0.680	0.426	0.679	0.944	-0.083	0.679	0.168	P01194|COLI_RAT	YGGFMTSEKSQTPLVTLFKNAIIKN
YKVNEYQGPVAPSGGFFLFRPRN(-.98)	0.790	0.869	0.973	0.985	0.942	-0.087	0.869	0.061	P12760|NMU_RAT	YKVNEYQGPVAPSGGFFLFRPRN(-.98)

Significant stat

Code

# get DE features only tibble
uni_res_filt <- uni_res %>%
        dplyr::filter(BHT < FDR & BHW < FDR) %>%
        mutate(status = case_when(`FC(log2)` < - 0.6 ~ "Down",
                                  `FC(log2)` > 0.6 ~ "Up") )

if (nrow(uni_res_filt) == 0){
        print(paste("There is no significant differentiated features between",
                    temp_d$Label[2], "and", temp_d$Label[1], sep = " "))
} else {
        uni_res_filt = uni_res_filt %>%
                left_join(., feature_meta, by = c("variable" = "Peptide"))
        
        #write.csv(uni_res_filt, file = paste(combo_list[[i]][1], "vs", combo_list[[i]][2], ".csv", sep = ""))
        uni_res_filt %>% 
                knitr::kable(., digits = 3, "html") %>%
                kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
                kableExtra::scroll_box(width = "100%", height = "500px")
}

variable	pT	BHT	pW	BHW	FC(lin)	FC(log2)	padj	-log10padj	status	Accession	org_peptide_name
FPAMPLSSLF	0.000	0.006	0.001	0.011	0.405	-1.305	0.006	2.207	Down	P01244|SOMA_RAT	FPAMPLSSLF
FPAMPLSSLFA	0.000	0.006	0.001	0.011	0.205	-2.286	0.006	2.207	Down	P01244|SOMA_RAT	FPAMPLSSLFA
FPAMPLSSLFAN	0.000	0.006	0.000	0.006	0.240	-2.058	0.006	2.253	Down	P01244|SOMA_RAT	FPAMPLSSLFAN
FPAMPLSSLFANA	0.001	0.020	0.002	0.026	0.238	-2.073	0.020	1.694	Down	P01244|SOMA_RAT	FPAMPLSSLFANA
FPAMPLSSLFANAVLR	0.000	0.003	0.000	0.002	0.225	-2.151	0.002	2.731	Down	P01244|SOMA_RAT	FPAMPLSSLFANAVLR
FPAMPLSSLFANAVLRA	0.001	0.014	0.001	0.016	0.306	-1.708	0.014	1.852	Down	P01244|SOMA_RAT	FPAMPLSSLFANAVLRA
FPAMPLSSLFANAVLRAQH	0.000	0.000	0.000	0.001	0.303	-1.722	0.000	3.817	Down	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQH
FPAMPLSSLFANAVLRAQHLH	0.000	0.002	0.000	0.004	0.406	-1.299	0.002	2.652	Down	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLH
FPAMPLSSLFANAVLRAQHLHQ	0.000	0.002	0.000	0.006	0.459	-1.122	0.002	2.652	Down	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQ
FPAMPLSSLFANAVLRAQHLHQLA	0.002	0.030	0.003	0.030	0.375	-1.414	0.030	1.525	Down	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQLA
FPAMPLSSLFANAVLRAQHLHQLAAD	0.001	0.023	0.002	0.026	0.391	-1.356	0.023	1.646	Down	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQLAAD
LDEGHDPVHESPV	0.002	0.023	0.000	0.007	2.336	1.224	0.007	2.156	Up	O35314|SCG1_RAT	LDEGHDPVHESPV
LLDEGHDPVHESPVDTA	0.002	0.025	0.001	0.016	1.855	0.891	0.016	1.785	Up	O35314|SCG1_RAT	LLDEGHDPVHESPVDTA
RTLYGFGG	0.003	0.036	0.003	0.030	0.546	-0.873	0.030	1.520	Down	P62804|H4_RAT	RTLYGFGG
VKVGVNGF	0.003	0.036	0.001	0.016	0.572	-0.807	0.016	1.785	Down	P04797|G3P_RAT	VKVGVNGF

Volcano plot

Code

# volcano plot
volcano_plot(uni_res_annotation, feature_col = "variable", fdr = FDR, log2fc = LOG2FC, save = FALSE)

Heatmap

Code

# heatmap
anno <- data.frame(Label = as.factor(t(d5)[, "class"])) %>%
        dplyr::filter(Label %in% contrast ) %>%
        droplevels()

hm <- d5[-1, d5[1,] %in% contrast ] %>%
        rownames_to_column("variable") %>%
        mutate(across(-variable, as.numeric)) #%>%
        #left_join(., feature_meta, by = c("variable" = "Feature"))

heatmap(hm, feature_col = "variable", sample_anno = anno, feature_anno = feature_meta, feature_label = "Peptide", rowname_switch = TRUE, save = FALSE)

Iso vs SP

Full stat

Code

contrast = combo_list[[2]]

df = d5_mod %>%
    dplyr::filter(., Label %in% contrast ) %>%
    droplevels()

temp_d <- df %>%
        mutate(across(-Label, ~ 2^(.) )) %>% # d3_mod is log-scale number, this step will de-log and change data back to linear number
        group_by(Label) %>%
        summarise(across(everything(), mean))

print(paste("Order of Fold Change:", temp_d$Label[1], "over", temp_d$Label[2], sep = " "))

[1] "Order of Fold Change: Iso over SP"

Code

uni_res = do_univariate(df, order = c("SP", "Iso"))

uni_res <- uni_res %>%
        rowwise() %>%
        mutate(padj = min(c(BHT, BHW))) %>%
        # get the lowest padj
        ungroup() %>%
        mutate(`-log10padj` = -log10(padj))

uni_res_annotation = uni_res %>% left_join(., feature_meta, by = c("variable" = "Peptide"))

uni_res_annotation %>% 
        #mutate(across(-variable, round, digit=3)) %>%
        knitr::kable(., digits = 3, "html") %>%
        kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
        kableExtra::scroll_box(width = "100%", height = "500px")

variable	pT	BHT	pW	BHW	FC(lin)	FC(log2)	padj	-log10padj	Accession	org_peptide_name
A(+42.01)AYKLVLIRH	0.059	0.192	0.076	0.222	0.688	-0.540	0.192	0.716	P25113|PGAM1_RAT	A(+42.01)AYKLVLIRH
A(+42.01)AYKLVLIRHGE	0.955	0.966	0.797	0.908	1.013	0.019	0.908	0.042	P25113|PGAM1_RAT	A(+42.01)AYKLVLIRHGE
AAFQKVVAGVASALAHKYH	0.077	0.223	0.088	0.240	0.618	-0.694	0.223	0.652	P02091|HBB1_RAT:P11517|HBB2_RAT	AAFQKVVAGVASALAHKYH
AEAFPLEF	0.067	0.201	0.076	0.222	1.724	0.786	0.201	0.697	P01194|COLI_RAT	AEAFPLEF
AEEETAGGDGRPEPSP	0.687	0.834	0.652	0.804	1.251	0.323	0.804	0.095	P01194|COLI_RAT	AEEETAGGDGRPEPSP
AEEETAGGDGRPEPSPR	0.820	0.888	1.000	1.000	1.037	0.053	0.888	0.051	P01194|COLI_RAT	AEEETAGGDGRPEPSPR
AEEETAGGDGRPEPSPRE(-.98)	0.922	0.945	0.438	0.625	1.100	0.137	0.625	0.204	P01194|COLI_RAT	AEEETAGGDGRPEPSPRE(-.98)
AFQKVVAGVASALAHKYH	0.766	0.873	0.652	0.804	0.795	-0.332	0.804	0.095	P02091|HBB1_RAT:P11517|HBB2_RAT	AFQKVVAGVASALAHKYH
AGTQESVDSAKP	0.040	0.163	0.040	0.156	1.951	0.965	0.156	0.807	P01186|NEU2_RAT	AGTQESVDSAKP
AGTQESVDSAKPRVY	0.639	0.798	0.401	0.592	1.338	0.420	0.592	0.227	P01186|NEU2_RAT	AGTQESVDSAKPRVY
AKVAVLGA	0.442	0.642	0.699	0.831	0.937	-0.093	0.642	0.192	P04636|MDHM_RAT	AKVAVLGA
AQAAFQKVVAGVASALAHKYH	0.025	0.120	0.047	0.172	0.589	-0.763	0.120	0.919	P02091|HBB1_RAT:P11517|HBB2_RAT	AQAAFQKVVAGVASALAHKYH
ASALAHKYH	0.335	0.545	0.401	0.592	1.317	0.398	0.545	0.264	P02091|HBB1_RAT:P11517|HBB2_RAT	ASALAHKYH
ASLDKFLASVSTVLTSKYR	0.006	0.050	0.013	0.081	0.433	-1.207	0.050	1.305	P01946|HBA_RAT	ASLDKFLASVSTVLTSKYR
AVDQDLGPEVPPENVL(-.98)	0.192	0.388	0.193	0.373	1.225	0.293	0.373	0.429	Q9QXU9|PCS1N_RAT	AVDQDLGPEVPPENVL(-.98)
DEGHDPVHESPVDTA	0.013	0.086	0.013	0.081	1.859	0.894	0.081	1.092	O35314|SCG1_RAT	DEGHDPVHESPVDTA
DPVHESPVDT	0.258	0.466	0.243	0.423	0.844	-0.245	0.423	0.373	O35314|SCG1_RAT	DPVHESPVDT
DPVHESPVDTA	0.625	0.795	0.949	0.966	1.041	0.058	0.795	0.100	O35314|SCG1_RAT	DPVHESPVDTA
EAFPLEF	0.095	0.250	0.151	0.326	1.585	0.665	0.250	0.602	P01194|COLI_RAT	EAFPLEF
EEETAGGDGRPEPSPRE(-.98)	0.676	0.834	0.332	0.518	1.029	0.041	0.518	0.286	P01194|COLI_RAT	EEETAGGDGRPEPSPRE(-.98)
EETAGGDGRPEPSPRE(-.98)	0.692	0.834	0.652	0.804	0.852	-0.231	0.804	0.095	P01194|COLI_RAT	EETAGGDGRPEPSPRE(-.98)
EGFFRLT	0.005	0.049	0.002	0.023	0.657	-0.605	0.023	1.630	P01186|NEU2_RAT	EGFFRLT
ELDQLLHY	0.867	0.923	0.606	0.777	0.890	-0.169	0.777	0.110	O35314|SCG1_RAT	ELDQLLHY
ELEGEQPD	0.080	0.224	0.151	0.326	1.386	0.470	0.224	0.650	P01194|COLI_RAT	ELEGEQPD
ESVDSAKPRVY	0.011	0.080	0.013	0.081	0.663	-0.593	0.080	1.098	P01186|NEU2_RAT	ESVDSAKPRVY
F(+42.01)PAMPLSSLFANAVLRAQHLH	0.738	0.869	0.748	0.857	1.235	0.304	0.857	0.067	P01244|SOMA_RAT	F(+42.01)PAMPLSSLFANAVLRAQHLH
FLASVSTVLTSKYR	0.222	0.420	0.606	0.777	0.812	-0.300	0.420	0.377	P01946|HBA_RAT	FLASVSTVLTSKYR
FMTSEKSQTP	0.179	0.380	0.101	0.256	1.489	0.574	0.256	0.592	P01194|COLI_RAT	FMTSEKSQTP
FNPYFDPL	0.330	0.545	0.217	0.395	1.121	0.165	0.395	0.403	O35314|SCG1_RAT	FNPYFDPL
FPAM(+15.99)PLSSLFANAVLR	0.753	0.869	0.898	0.956	0.842	-0.249	0.869	0.061	P01244|SOMA_RAT	FPAM(+15.99)PLSSLFANAVLR
FPAM(+15.99)PLSSLFANAVLRAQH	0.637	0.798	0.699	0.831	0.833	-0.263	0.798	0.098	P01244|SOMA_RAT	FPAM(+15.99)PLSSLFANAVLRAQH
FPAM(+15.99)PLSSLFANAVLRAQHLH	0.055	0.192	0.065	0.210	0.480	-1.060	0.192	0.716	P01244|SOMA_RAT	FPAM(+15.99)PLSSLFANAVLRAQHLH
FPAMPLS	0.293	0.507	0.193	0.373	0.747	-0.421	0.373	0.429	P01244|SOMA_RAT	FPAMPLS
FPAMPLSS	0.064	0.201	0.076	0.222	0.622	-0.685	0.201	0.697	P01244|SOMA_RAT	FPAMPLSS
FPAMPLSSL	0.014	0.087	0.028	0.144	0.557	-0.844	0.087	1.059	P01244|SOMA_RAT	FPAMPLSSL
FPAMPLSSLF	0.041	0.163	0.040	0.156	0.557	-0.844	0.156	0.807	P01244|SOMA_RAT	FPAMPLSSLF
FPAMPLSSLFA	0.003	0.035	0.002	0.023	0.295	-1.761	0.023	1.630	P01244|SOMA_RAT	FPAMPLSSLFA
FPAMPLSSLFAN	0.002	0.029	0.002	0.025	0.323	-1.630	0.025	1.601	P01244|SOMA_RAT	FPAMPLSSLFAN
FPAMPLSSLFANA	0.996	0.999	0.478	0.664	0.473	-1.080	0.664	0.178	P01244|SOMA_RAT	FPAMPLSSLFANA
FPAMPLSSLFANAVLR	0.003	0.035	0.007	0.054	0.363	-1.463	0.035	1.455	P01244|SOMA_RAT	FPAMPLSSLFANAVLR
FPAMPLSSLFANAVLRA	0.515	0.687	0.332	0.518	0.728	-0.458	0.518	0.286	P01244|SOMA_RAT	FPAMPLSSLFANAVLRA
FPAMPLSSLFANAVLRAQ	0.813	0.888	0.748	0.857	0.867	-0.207	0.857	0.067	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQ
FPAMPLSSLFANAVLRAQH	0.000	0.001	0.000	0.002	0.414	-1.274	0.001	3.163	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQH
FPAMPLSSLFANAVLRAQHLH	0.001	0.023	0.001	0.019	0.481	-1.056	0.019	1.722	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLH
FPAMPLSSLFANAVLRAQHLHQ	0.000	0.012	0.000	0.010	0.539	-0.892	0.010	1.984	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQ
FPAMPLSSLFANAVLRAQHLHQL	0.378	0.593	0.151	0.326	0.739	-0.437	0.326	0.486	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQL
FPAMPLSSLFANAVLRAQHLHQLA	0.087	0.234	0.040	0.156	0.694	-0.528	0.156	0.807	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQLA
FPAMPLSSLFANAVLRAQHLHQLAAD	0.442	0.642	0.519	0.703	0.916	-0.127	0.642	0.192	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQLAAD
FPKPEGSQD	0.106	0.268	0.151	0.326	1.345	0.427	0.268	0.572	P47868|SCG3_RAT	FPKPEGSQD
FPKPEGSQDKSLHN	0.223	0.420	0.438	0.625	0.768	-0.380	0.420	0.377	P47868|SCG3_RAT	FPKPEGSQDKSLHN
GPNLVARSKEALA	0.019	0.113	0.040	0.156	0.569	-0.812	0.113	0.948	P02770|ALBU_RAT	GPNLVARSKEALA
GPVQFLSRIF	0.434	0.641	0.332	0.518	1.195	0.257	0.518	0.286	P01244|SOMA_RAT	GPVQFLSRIF
GPYRVEHF	0.112	0.273	0.047	0.172	1.329	0.411	0.172	0.763	P01194|COLI_RAT	GPYRVEHF
GPYRVEHFRWGNPPKD	0.596	0.764	0.847	0.945	1.208	0.273	0.764	0.117	P01194|COLI_RAT	GPYRVEHFRWGNPPKD
GRGREPGAYPALDSRQE	0.380	0.593	0.217	0.395	1.245	0.316	0.395	0.403	O35314|SCG1_RAT	GRGREPGAYPALDSRQE
GRTLYGFGG	0.128	0.295	0.065	0.210	0.748	-0.418	0.210	0.679	P62804|H4_RAT	GRTLYGFGG
GTQESVDSAKPRVY	0.747	0.869	0.652	0.804	1.071	0.100	0.804	0.095	P01186|NEU2_RAT	GTQESVDSAKPRVY
GVASALAHKYH	0.011	0.080	0.019	0.109	0.506	-0.982	0.080	1.098	P02091|HBB1_RAT:P11517|HBB2_RAT	GVASALAHKYH
IKMALQQEGFD	0.792	0.888	0.332	0.518	0.620	-0.689	0.518	0.286	P28841|NEC2_RAT	IKMALQQEGFD
KYVMGHF	0.183	0.381	0.151	0.326	1.242	0.312	0.326	0.486	P01194|COLI_RAT	KYVMGHF
KYVMGHFRWD	0.087	0.234	0.101	0.256	1.663	0.734	0.234	0.631	P01194|COLI_RAT	KYVMGHFRWD
LDEGHDPV	0.001	0.023	0.001	0.018	2.018	1.013	0.018	1.746	O35314|SCG1_RAT	LDEGHDPV
LDEGHDPVHESPV	0.002	0.029	0.004	0.037	2.057	1.040	0.029	1.540	O35314|SCG1_RAT	LDEGHDPVHESPV
LENSSPQAPA	0.062	0.199	0.076	0.222	1.548	0.631	0.199	0.702	Q9QXU9|PCS1N_RAT	LENSSPQAPA
LENSSPQAPARRLLPP	0.115	0.273	0.056	0.186	1.700	0.765	0.186	0.730	Q9QXU9|PCS1N_RAT	LENSSPQAPARRLLPP
LLDEGHDPVHESPVD	0.276	0.485	0.088	0.240	1.345	0.428	0.240	0.619	O35314|SCG1_RAT	LLDEGHDPVHESPVD
LLDEGHDPVHESPVDT	0.161	0.351	0.040	0.156	1.439	0.525	0.156	0.807	O35314|SCG1_RAT	LLDEGHDPVHESPVDT
LLDEGHDPVHESPVDTA	0.002	0.025	0.001	0.019	1.833	0.874	0.019	1.722	O35314|SCG1_RAT	LLDEGHDPVHESPVDTA
LLRALASSNARAQQ	0.013	0.086	0.004	0.037	1.707	0.771	0.037	1.427	Q792G6|GNAS3_RAT	LLRALASSNARAQQ
LPGLLLF	0.901	0.941	0.748	0.857	0.855	-0.226	0.857	0.067	P01230|LSHB_RAT	LPGLLLF
LPHLPGLLLF	0.891	0.937	0.898	0.956	0.876	-0.191	0.937	0.028	P01230|LSHB_RAT	LPHLPGLLLF
LVQLAGTQESVDSAKPRVY	0.349	0.560	0.365	0.560	1.333	0.414	0.560	0.252	P01186|NEU2_RAT	LVQLAGTQESVDSAKPRVY
MDQLAKELT	0.036	0.157	0.116	0.281	1.435	0.521	0.157	0.803	P10354|CMGA_RAT	MDQLAKELT
MDQLAKELTAE	0.237	0.436	0.300	0.487	1.505	0.590	0.436	0.360	P10354|CMGA_RAT	MDQLAKELTAE
MQIFVKTL	0.278	0.485	0.300	0.487	0.704	-0.505	0.485	0.314	P62982|RS27A_RAT:P62986|RL40_RAT:P0CG51|UBB_RAT:Q63429|UBC_RAT	MQIFVKTL
NPYFDPLQWKNSDFE	0.478	0.659	0.401	0.592	0.867	-0.205	0.592	0.227	O35314|SCG1_RAT	NPYFDPLQWKNSDFE
NQEQAEQGREHL	0.750	0.869	0.898	0.956	0.971	-0.043	0.869	0.061	P10362|SCG2_RAT	NQEQAEQGREHL
PESAFSE	0.001	0.023	0.001	0.015	1.948	0.962	0.015	1.821	P01179|NEU1_RAT	PESAFSE
PHFLYPK	0.036	0.157	0.034	0.156	1.480	0.566	0.156	0.807	P05371|CLUS_RAT	PHFLYPK
PMFIVNTNVPRA	0.485	0.662	0.562	0.749	0.898	-0.156	0.662	0.179	P30904|MIF_RAT	PMFIVNTNVPRA
PVHESPVDTA	0.002	0.029	0.001	0.015	2.250	1.170	0.015	1.821	O35314|SCG1_RAT	PVHESPVDTA
PVQFLSRIF	0.878	0.929	0.949	0.966	1.118	0.162	0.929	0.032	P01244|SOMA_RAT	PVQFLSRIF
Q(-17.03)ELGKLTGPSNQ	0.115	0.273	0.193	0.373	1.277	0.352	0.273	0.564	P10362|SCG2_RAT	Q(-17.03)ELGKLTGPSNQ
Q(-17.03)EPGAPAAGM	0.558	0.732	0.949	0.966	0.935	-0.097	0.732	0.135	P15087|CBPE_RAT	Q(-17.03)EPGAPAAGM
Q(-17.03)EPGAPAAGM(+15.99)	0.024	0.120	0.013	0.081	1.505	0.590	0.081	1.092	P15087|CBPE_RAT	Q(-17.03)EPGAPAAGM(+15.99)
Q(-17.03)KIAEKFSQ	0.000	0.012	0.000	0.010	2.080	1.057	0.010	1.984	O35314|SCG1_RAT	Q(-17.03)KIAEKFSQ
Q(-17.03)KIAEKFSQR(-.98)	0.009	0.070	0.008	0.065	1.614	0.691	0.065	1.187	O35314|SCG1_RAT	Q(-17.03)KIAEKFSQR(-.98)
QAAFQKVVAGVASALAHKYH	0.325	0.544	0.478	0.664	0.732	-0.450	0.544	0.264	P02091|HBB1_RAT:P11517|HBB2_RAT	QAAFQKVVAGVASALAHKYH
R(+42.01)PVKVYPNVAENESAEAFPLEF	0.025	0.120	0.056	0.186	2.232	1.159	0.120	0.919	P01194|COLI_RAT	R(+42.01)PVKVYPNVAENESAEAFPLEF
RAEEETAGGDGRPEPSPRE(-.98)	0.469	0.659	0.606	0.777	1.776	0.829	0.659	0.181	P01194|COLI_RAT	RAEEETAGGDGRPEPSPRE(-.98)
RPVKVYPNVAENE	0.271	0.483	0.270	0.462	1.445	0.531	0.462	0.336	P01194|COLI_RAT	RPVKVYPNVAENE
RPVKVYPNVAENES(+79.97)AEAFPLE	0.416	0.632	0.562	0.749	1.122	0.167	0.632	0.200	P01194|COLI_RAT	RPVKVYPNVAENES(+79.97)AEAFPLE
RPVKVYPNVAENES(+79.97)AEAFPLEF	0.464	0.659	0.243	0.423	1.224	0.292	0.423	0.373	P01194|COLI_RAT	RPVKVYPNVAENES(+79.97)AEAFPLEF
RPVKVYPNVAENESAEAFPLE	0.139	0.312	0.217	0.395	1.342	0.424	0.312	0.506	P01194|COLI_RAT	RPVKVYPNVAENESAEAFPLE
RPVKVYPNVAENESAEAFPLEF	0.478	0.659	0.699	0.831	1.028	0.040	0.659	0.181	P01194|COLI_RAT	RPVKVYPNVAENESAEAFPLEF
RRPVKVYPNVAENESAEAFPLEF	0.563	0.733	0.519	0.703	1.038	0.054	0.703	0.153	P01194|COLI_RAT	RRPVKVYPNVAENESAEAFPLEF
RTLYGFGG	0.035	0.157	0.019	0.109	0.646	-0.630	0.109	0.964	P62804|H4_RAT	RTLYGFGG
S(+42.01)MEHFRWGKPV(-.98)	0.098	0.256	0.101	0.256	1.740	0.799	0.256	0.592	P01194|COLI_RAT	S(+42.01)MEHFRWGKPV(-.98)
S(+42.01)YSMEHF	0.950	0.966	0.898	0.956	0.874	-0.195	0.956	0.020	P01194|COLI_RAT	S(+42.01)YSMEHF
S(+42.01)YSMEHFRWGKPV(-.98)	0.806	0.888	0.898	0.956	1.039	0.056	0.888	0.051	P01194|COLI_RAT	S(+42.01)YSMEHFRWGKPV(-.98)
SAEAFPLEF	0.910	0.943	1.000	1.000	0.770	-0.378	0.943	0.026	P01194|COLI_RAT	SAEAFPLEF
SAVISLEGKPL	0.137	0.312	0.217	0.395	0.774	-0.369	0.312	0.506	P45592|COF1_RAT	SAVISLEGKPL
SEES(+79.97)QEKEY	0.218	0.420	0.300	0.487	1.246	0.318	0.420	0.377	O35314|SCG1_RAT	SEES(+79.97)QEKEY
SLDKFLA	0.808	0.888	0.847	0.945	0.517	-0.951	0.888	0.051	P01946|HBA_RAT	SLDKFLA
SLDKFLASVSTVLTSKYR	0.401	0.620	0.300	0.487	1.046	0.064	0.487	0.312	P01946|HBA_RAT	SLDKFLASVSTVLTSKYR
SLKPEFVDIINAKQ	0.044	0.167	0.040	0.156	0.614	-0.705	0.156	0.807	P48500|TPIS_RAT	SLKPEFVDIINAKQ
SLSAASAPLAETSTPL	0.037	0.157	0.101	0.256	1.352	0.435	0.157	0.803	Q9QXU9|PCS1N_RAT	SLSAASAPLAETSTPL
SLSAASAPLAETSTPLRL	0.022	0.120	0.040	0.156	1.646	0.719	0.120	0.919	Q9QXU9|PCS1N_RAT	SLSAASAPLAETSTPLRL
SMEHFRWGKPV(-.98)	0.170	0.366	0.193	0.373	1.407	0.493	0.366	0.437	P01194|COLI_RAT	SMEHFRWGKPV(-.98)
SNRVVDLMAYMASKE	0.008	0.064	0.002	0.025	0.439	-1.189	0.025	1.601	P04797|G3P_RAT	SNRVVDLMAYMASKE
SPRIGQILK	0.056	0.192	0.076	0.222	0.616	-0.698	0.192	0.716	P01244|SOMA_RAT	SPRIGQILK
SPRIGQILKQ	0.186	0.381	0.171	0.351	1.420	0.506	0.351	0.454	P01244|SOMA_RAT	SPRIGQILKQ
SQENPNTYSEDLDV	0.336	0.545	0.519	0.703	1.243	0.314	0.545	0.264	P06300|PDYN_RAT	SQENPNTYSEDLDV
SSEPEAAPAPRRL	0.214	0.418	0.217	0.395	1.189	0.250	0.395	0.403	Q9QXU9|PCS1N_RAT	SSEPEAAPAPRRL
SVDSAKPRVY	0.829	0.888	0.949	0.966	1.023	0.033	0.888	0.051	P01186|NEU2_RAT	SVDSAKPRVY
SVNPYLQ(-.98)	0.200	0.400	0.243	0.423	1.399	0.485	0.400	0.398	P27682|7B2_RAT	SVNPYLQ(-.98)
SVNPYLQGKRLDNVV	0.451	0.649	0.606	0.777	1.169	0.226	0.649	0.188	P27682|7B2_RAT	SVNPYLQGKRLDNVV
SVNPYLQGKRLDNVVA	0.081	0.224	0.116	0.281	1.260	0.333	0.224	0.650	P27682|7B2_RAT	SVNPYLQGKRLDNVVA
SVPHFSEEEKEPE	0.001	0.025	0.000	0.015	1.737	0.796	0.015	1.821	P27682|7B2_RAT	SVPHFSEEEKEPE
SVSTVLTSKYR	0.022	0.120	0.023	0.123	0.504	-0.989	0.120	0.919	P01946|HBA_RAT	SVSTVLTSKYR
SYSMEHF	0.425	0.633	0.365	0.560	0.864	-0.211	0.560	0.252	P01194|COLI_RAT	SYSMEHF
SYSMEHFRWGKPV(-.98)	0.477	0.659	0.438	0.625	1.175	0.232	0.625	0.204	P01194|COLI_RAT	SYSMEHFRWGKPV(-.98)
TAYAIYMLAMAAFPKKQN	0.642	0.798	0.699	0.831	0.886	-0.174	0.798	0.098	P35171|CX7A2_RAT	TAYAIYMLAMAAFPKKQN
TDMELLRF	0.418	0.632	0.699	0.831	0.597	-0.744	0.632	0.200	P01244|SOMA_RAT	TDMELLRF
TLYGFGG	0.687	0.834	0.748	0.857	0.932	-0.102	0.834	0.079	P62804|H4_RAT	TLYGFGG
TPAMHASLDKFLASVSTVLTSKYR	0.003	0.035	0.002	0.025	0.324	-1.627	0.025	1.601	P01946|HBA_RAT	TPAMHASLDKFLASVSTVLTSKYR
TPLPELFDRVVML	0.789	0.888	0.652	0.804	0.758	-0.400	0.804	0.095	P01237|PRL_RAT	TPLPELFDRVVML
TPLVTLF	0.109	0.270	0.088	0.240	1.560	0.642	0.240	0.619	P01194|COLI_RAT	TPLVTLF
TPLVTLFKNAIIKN	0.225	0.420	0.171	0.351	1.154	0.206	0.351	0.454	P01194|COLI_RAT	TPLVTLFKNAIIKN
TQESVDSAKPRVY	0.307	0.524	0.270	0.462	0.887	-0.172	0.462	0.336	P01186|NEU2_RAT	TQESVDSAKPRVY
VHLTDAEKA	0.154	0.342	0.171	0.351	0.504	-0.988	0.342	0.466	P02091|HBB1_RAT:P11517|HBB2_RAT	VHLTDAEKA
VHLTDAEKAAVN	0.057	0.192	0.088	0.240	0.679	-0.559	0.192	0.716	P02091|HBB1_RAT	VHLTDAEKAAVN
VHLTDAEKAAVNG	0.211	0.416	0.401	0.592	0.859	-0.219	0.416	0.381	P02091|HBB1_RAT	VHLTDAEKAAVNG
VKVGVNGF	0.001	0.025	0.001	0.023	0.498	-1.006	0.023	1.639	P04797|G3P_RAT	VKVGVNGF
VKVGVNGFGRIG	0.126	0.295	0.151	0.326	0.722	-0.469	0.295	0.530	P04797|G3P_RAT	VKVGVNGFGRIG
VPHFSEEEKEPE	0.999	0.999	1.000	1.000	1.050	0.070	0.999	0.000	P27682|7B2_RAT	VPHFSEEEKEPE
VQLAGTQESVDSAKP	0.050	0.188	0.047	0.172	1.648	0.721	0.172	0.763	P01186|NEU2_RAT	VQLAGTQESVDSAKP
VQLAGTQESVDSAKPR	0.544	0.719	0.171	0.351	1.499	0.584	0.351	0.454	P01186|NEU2_RAT	VQLAGTQESVDSAKPR
VQLAGTQESVDSAKPRV	0.067	0.201	0.101	0.256	2.319	1.214	0.201	0.697	P01186|NEU2_RAT	VQLAGTQESVDSAKPRV
VQLAGTQESVDSAKPRVY	0.825	0.888	0.847	0.945	1.144	0.194	0.888	0.051	P01186|NEU2_RAT	VQLAGTQESVDSAKPRVY
WSRMDQLAKELT	0.352	0.560	0.300	0.487	1.300	0.379	0.487	0.312	P10354|CMGA_RAT	WSRMDQLAKELT
WSRMDQLAKELTAE	0.052	0.188	0.056	0.186	1.507	0.592	0.186	0.730	P10354|CMGA_RAT	WSRMDQLAKELTAE
Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLF	0.044	0.167	0.056	0.186	2.155	1.108	0.167	0.777	P01194|COLI_RAT	Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLF
Y(+42.01)GGFMTSEK	0.762	0.873	0.898	0.956	0.911	-0.135	0.873	0.059	P01194|COLI_RAT	Y(+42.01)GGFMTSEK
Y(+42.01)GGFMTSEKSQ	0.815	0.888	0.898	0.956	0.967	-0.049	0.888	0.051	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQ
Y(+42.01)GGFMTSEKSQTP	0.004	0.038	0.007	0.054	1.969	0.977	0.038	1.420	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTP
Y(+42.01)GGFMTSEKSQTPL	0.102	0.261	0.133	0.316	1.331	0.412	0.261	0.583	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPL
Y(+42.01)GGFMTSEKSQTPLV	0.322	0.544	0.438	0.625	1.157	0.210	0.544	0.264	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLV
Y(+42.01)GGFMTSEKSQTPLVT	0.420	0.632	0.478	0.664	1.045	0.064	0.632	0.200	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVT
Y(+42.01)GGFMTSEKSQTPLVTL	0.568	0.734	0.949	0.966	0.996	-0.006	0.734	0.134	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVTL
Y(+42.01)GGFMTSEKSQTPLVTLF	0.066	0.201	0.116	0.281	1.101	0.138	0.201	0.697	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVTLF
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIK	0.241	0.439	0.193	0.373	1.040	0.057	0.373	0.429	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIK
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKN	0.509	0.687	0.243	0.423	0.992	-0.012	0.423	0.373	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKN
Y(+42.01)SMEHFRWGKPV(-.98)	0.001	0.025	0.002	0.023	2.444	1.289	0.023	1.630	P01194|COLI_RAT	Y(+42.01)SMEHFRWGKPV(-.98)
YEENSRENPF	0.037	0.157	0.023	0.123	1.781	0.833	0.123	0.909	P10362|SCG2_RAT	YEENSRENPF
YGGFLRKYP	0.023	0.120	0.019	0.109	1.926	0.946	0.109	0.964	P06300|PDYN_RAT	YGGFLRKYP
YGGFLRKYPK	0.077	0.223	0.034	0.156	1.715	0.778	0.156	0.807	P06300|PDYN_RAT	YGGFLRKYPK
YGGFLRRI	0.059	0.192	0.040	0.156	1.339	0.421	0.156	0.807	P06300|PDYN_RAT	YGGFLRRI
YGGFLRRQFKVVT	0.186	0.381	0.151	0.326	1.264	0.338	0.326	0.486	P06300|PDYN_RAT	YGGFLRRQFKVVT
YGGFMTSEKSQTPLVT	0.512	0.687	0.748	0.857	1.058	0.081	0.687	0.163	P01194|COLI_RAT	YGGFMTSEKSQTPLVT
YGGFMTSEKSQTPLVTL	0.019	0.113	0.013	0.081	2.099	1.070	0.081	1.092	P01194|COLI_RAT	YGGFMTSEKSQTPLVTL
YGGFMTSEKSQTPLVTLF	0.914	0.943	0.949	0.966	1.060	0.084	0.943	0.026	P01194|COLI_RAT	YGGFMTSEKSQTPLVTLF
YGGFMTSEKSQTPLVTLFKNAIIKN	0.704	0.837	0.949	0.966	0.781	-0.356	0.837	0.077	P01194|COLI_RAT	YGGFMTSEKSQTPLVTLFKNAIIKN
YKVNEYQGPVAPSGGFFLFRPRN(-.98)	0.703	0.837	0.606	0.777	0.867	-0.206	0.777	0.110	P12760|NMU_RAT	YKVNEYQGPVAPSGGFFLFRPRN(-.98)

Significant stat

Code

# get DE features only tibble
uni_res_filt <- uni_res %>%
        dplyr::filter(BHT < FDR & BHW < FDR) %>%
        mutate(status = case_when(`FC(log2)` < - 0.6 ~ "Down",
                                  `FC(log2)` > 0.6 ~ "Up") )

if (nrow(uni_res_filt) == 0){
        print(paste("There is no significant differentiated features between",
                    temp_d$Label[2], "and", temp_d$Label[1], sep = " "))
} else {
        uni_res_filt = uni_res_filt %>%
                left_join(., feature_meta, by = c("variable" = "Peptide"))
        
        #write.csv(uni_res_filt, file = paste(combo_list[[i]][1], "vs", combo_list[[i]][2], ".csv", sep = ""))
        uni_res_filt %>% 
                #mutate(across(-c(variable, status), round, digit=3)) %>%
                knitr::kable(., digits = 3, "html") %>%
                kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
                kableExtra::scroll_box(width = "100%", height = "500px")
}

variable	pT	BHT	pW	BHW	FC(lin)	FC(log2)	padj	-log10padj	status	Accession	org_peptide_name
EGFFRLT	0.005	0.049	0.002	0.023	0.657	-0.605	0.023	1.630	Down	P01186|NEU2_RAT	EGFFRLT
FPAMPLSSLFA	0.003	0.035	0.002	0.023	0.295	-1.761	0.023	1.630	Down	P01244|SOMA_RAT	FPAMPLSSLFA
FPAMPLSSLFAN	0.002	0.029	0.002	0.025	0.323	-1.630	0.025	1.601	Down	P01244|SOMA_RAT	FPAMPLSSLFAN
FPAMPLSSLFANAVLRAQH	0.000	0.001	0.000	0.002	0.414	-1.274	0.001	3.163	Down	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQH
FPAMPLSSLFANAVLRAQHLH	0.001	0.023	0.001	0.019	0.481	-1.056	0.019	1.722	Down	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLH
FPAMPLSSLFANAVLRAQHLHQ	0.000	0.012	0.000	0.010	0.539	-0.892	0.010	1.984	Down	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQ
LDEGHDPV	0.001	0.023	0.001	0.018	2.018	1.013	0.018	1.746	Up	O35314|SCG1_RAT	LDEGHDPV
LDEGHDPVHESPV	0.002	0.029	0.004	0.037	2.057	1.040	0.029	1.540	Up	O35314|SCG1_RAT	LDEGHDPVHESPV
LLDEGHDPVHESPVDTA	0.002	0.025	0.001	0.019	1.833	0.874	0.019	1.722	Up	O35314|SCG1_RAT	LLDEGHDPVHESPVDTA
PESAFSE	0.001	0.023	0.001	0.015	1.948	0.962	0.015	1.821	Up	P01179|NEU1_RAT	PESAFSE
PVHESPVDTA	0.002	0.029	0.001	0.015	2.250	1.170	0.015	1.821	Up	O35314|SCG1_RAT	PVHESPVDTA
Q(-17.03)KIAEKFSQ	0.000	0.012	0.000	0.010	2.080	1.057	0.010	1.984	Up	O35314|SCG1_RAT	Q(-17.03)KIAEKFSQ
SVPHFSEEEKEPE	0.001	0.025	0.000	0.015	1.737	0.796	0.015	1.821	Up	P27682|7B2_RAT	SVPHFSEEEKEPE
TPAMHASLDKFLASVSTVLTSKYR	0.003	0.035	0.002	0.025	0.324	-1.627	0.025	1.601	Down	P01946|HBA_RAT	TPAMHASLDKFLASVSTVLTSKYR
VKVGVNGF	0.001	0.025	0.001	0.023	0.498	-1.006	0.023	1.639	Down	P04797|G3P_RAT	VKVGVNGF
Y(+42.01)SMEHFRWGKPV(-.98)	0.001	0.025	0.002	0.023	2.444	1.289	0.023	1.630	Up	P01194|COLI_RAT	Y(+42.01)SMEHFRWGKPV(-.98)

Volcano plot

Code

# volcano plot
volcano_plot(uni_res_annotation, feature_col = "variable", fdr = FDR, log2fc = LOG2FC, save = FALSE)

Heatmap

Code

# heatmap
anno <- data.frame(Label = as.factor(t(d5)[, "class"])) %>%
        dplyr::filter(Label %in% contrast ) %>%
        droplevels()

hm <- d5[-1, d5[1,] %in% contrast ] %>%
        rownames_to_column("variable") %>%
        mutate(across(-variable, as.numeric)) #%>%
        #left_join(., feature_meta, by = c("variable" = "Feature"))
        
heatmap(hm, feature_col = "variable", sample_anno = anno, feature_anno = feature_meta, feature_label = "Peptide", rowname_switch = TRUE, save = FALSE)

No vs SP

Full stat

Code

contrast = combo_list[[3]]

df = d5_mod %>%
    dplyr::filter(., Label %in% contrast ) %>%
    droplevels()

temp_d <- df %>%
        mutate(across(-Label, ~ 2^(.) )) %>% # d3_mod is log-scale number, this step will de-log and change data back to linear number
        group_by(Label) %>%
        summarise(across(everything(), mean))

print(paste("Order of Fold Change:", temp_d$Label[2], "over", temp_d$Label[1], sep = " "))

[1] "Order of Fold Change: SP over No"

Code

uni_res = do_univariate(df, order = c("No", "SP"))

uni_res <- uni_res %>%
        rowwise() %>%
        mutate(padj = min(c(BHT, BHW))) %>%
        # get the lowest padj
        ungroup() %>%
        mutate(`-log10padj` = -log10(padj))

uni_res_annotation = uni_res %>% left_join(., feature_meta, by = c("variable" = "Peptide"))

uni_res_annotation %>% 
        #mutate(across(-variable, round, digit=3)) %>%
        knitr::kable(., digits = 3, "html") %>%
        kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
        kableExtra::scroll_box(width = "100%", height = "500px")

variable	pT	BHT	pW	BHW	FC(lin)	FC(log2)	padj	-log10padj	Accession	org_peptide_name
A(+42.01)AYKLVLIRH	0.030	0.483	0.013	0.348	1.580	0.660	0.348	0.459	P25113|PGAM1_RAT	A(+42.01)AYKLVLIRH
A(+42.01)AYKLVLIRHGE	0.642	0.973	0.756	0.984	1.245	0.316	0.973	0.012	P25113|PGAM1_RAT	A(+42.01)AYKLVLIRHGE
AAFQKVVAGVASALAHKYH	0.164	0.659	0.251	0.909	1.465	0.551	0.659	0.181	P02091|HBB1_RAT:P11517|HBB2_RAT	AAFQKVVAGVASALAHKYH
AEAFPLEF	0.329	0.868	0.282	0.909	0.758	-0.400	0.868	0.062	P01194|COLI_RAT	AEAFPLEF
AEEETAGGDGRPEPSP	0.464	0.874	0.756	0.984	0.914	-0.129	0.874	0.058	P01194|COLI_RAT	AEEETAGGDGRPEPSP
AEEETAGGDGRPEPSPR	0.395	0.868	0.557	0.963	0.837	-0.257	0.868	0.062	P01194|COLI_RAT	AEEETAGGDGRPEPSPR
AEEETAGGDGRPEPSPRE(-.98)	0.584	0.933	0.557	0.963	0.878	-0.188	0.933	0.030	P01194|COLI_RAT	AEEETAGGDGRPEPSPRE(-.98)
AFQKVVAGVASALAHKYH	0.944	0.979	0.705	0.984	1.218	0.284	0.979	0.009	P02091|HBB1_RAT:P11517|HBB2_RAT	AFQKVVAGVASALAHKYH
AGTQESVDSAKP	0.055	0.496	0.072	0.562	0.716	-0.482	0.496	0.304	P01186|NEU2_RAT	AGTQESVDSAKP
AGTQESVDSAKPRVY	0.131	0.612	0.132	0.637	0.729	-0.455	0.612	0.214	P01186|NEU2_RAT	AGTQESVDSAKPRVY
AKVAVLGA	0.529	0.885	0.512	0.963	0.872	-0.197	0.885	0.053	P04636|MDHM_RAT	AKVAVLGA
AQAAFQKVVAGVASALAHKYH	0.061	0.496	0.043	0.503	1.488	0.573	0.496	0.304	P02091|HBB1_RAT:P11517|HBB2_RAT	AQAAFQKVVAGVASALAHKYH
ASALAHKYH	0.683	0.973	0.863	0.984	0.913	-0.131	0.973	0.012	P02091|HBB1_RAT:P11517|HBB2_RAT	ASALAHKYH
ASLDKFLASVSTVLTSKYR	0.369	0.868	0.605	0.963	1.155	0.208	0.868	0.062	P01946|HBA_RAT	ASLDKFLASVSTVLTSKYR
AVDQDLGPEVPPENVL(-.98)	0.242	0.779	0.099	0.622	0.720	-0.475	0.622	0.206	Q9QXU9|PCS1N_RAT	AVDQDLGPEVPPENVL(-.98)
DEGHDPVHESPVDTA	0.292	0.866	0.512	0.963	0.867	-0.206	0.866	0.062	O35314|SCG1_RAT	DEGHDPVHESPVDTA
DPVHESPVDT	0.457	0.874	0.512	0.963	1.101	0.138	0.874	0.058	O35314|SCG1_RAT	DPVHESPVDT
DPVHESPVDTA	0.958	0.979	0.756	0.984	1.063	0.088	0.979	0.009	O35314|SCG1_RAT	DPVHESPVDTA
EAFPLEF	0.049	0.496	0.061	0.556	0.613	-0.706	0.496	0.304	P01194|COLI_RAT	EAFPLEF
EEETAGGDGRPEPSPRE(-.98)	0.409	0.868	0.605	0.963	0.863	-0.212	0.868	0.062	P01194|COLI_RAT	EEETAGGDGRPEPSPRE(-.98)
EETAGGDGRPEPSPRE(-.98)	0.157	0.659	0.061	0.556	0.557	-0.844	0.556	0.255	P01194|COLI_RAT	EETAGGDGRPEPSPRE(-.98)
EGFFRLT	0.394	0.868	0.512	0.963	1.117	0.159	0.868	0.062	P01186|NEU2_RAT	EGFFRLT
ELDQLLHY	0.979	0.991	0.973	0.985	1.092	0.127	0.985	0.007	O35314|SCG1_RAT	ELDQLLHY
ELEGEQPD	0.130	0.612	0.114	0.626	0.750	-0.416	0.612	0.214	P01194|COLI_RAT	ELEGEQPD
ESVDSAKPRVY	0.992	0.993	0.918	0.984	0.890	-0.169	0.984	0.007	P01186|NEU2_RAT	ESVDSAKPRVY
F(+42.01)PAMPLSSLFANAVLRAQHLH	0.507	0.878	0.863	0.984	0.683	-0.550	0.878	0.057	P01244|SOMA_RAT	F(+42.01)PAMPLSSLFANAVLRAQHLH
FLASVSTVLTSKYR	0.492	0.876	0.654	0.975	1.114	0.156	0.876	0.057	P01946|HBA_RAT	FLASVSTVLTSKYR
FMTSEKSQTP	0.374	0.868	0.468	0.959	0.844	-0.244	0.868	0.062	P01194|COLI_RAT	FMTSEKSQTP
FNPYFDPL	0.382	0.868	0.426	0.932	0.799	-0.324	0.868	0.062	O35314|SCG1_RAT	FNPYFDPL
FPAM(+15.99)PLSSLFANAVLR	0.165	0.659	0.251	0.909	0.598	-0.743	0.659	0.181	P01244|SOMA_RAT	FPAM(+15.99)PLSSLFANAVLR
FPAM(+15.99)PLSSLFANAVLRAQH	0.229	0.773	0.282	0.909	0.727	-0.460	0.773	0.112	P01244|SOMA_RAT	FPAM(+15.99)PLSSLFANAVLRAQH
FPAM(+15.99)PLSSLFANAVLRAQHLH	0.844	0.979	0.918	0.984	1.006	0.008	0.979	0.009	P01244|SOMA_RAT	FPAM(+15.99)PLSSLFANAVLRAQHLH
FPAMPLS	0.923	0.979	0.973	0.985	1.003	0.004	0.979	0.009	P01244|SOMA_RAT	FPAMPLS
FPAMPLSS	0.408	0.868	0.557	0.963	1.298	0.376	0.868	0.062	P01244|SOMA_RAT	FPAMPLSS
FPAMPLSSL	0.767	0.973	0.918	0.984	1.118	0.161	0.973	0.012	P01244|SOMA_RAT	FPAMPLSSL
FPAMPLSSLF	0.097	0.552	0.114	0.626	0.726	-0.461	0.552	0.258	P01244|SOMA_RAT	FPAMPLSSLF
FPAMPLSSLFA	0.278	0.860	0.282	0.909	0.695	-0.525	0.860	0.066	P01244|SOMA_RAT	FPAMPLSSLFA
FPAMPLSSLFAN	0.307	0.866	0.468	0.959	0.744	-0.427	0.866	0.062	P01244|SOMA_RAT	FPAMPLSSLFAN
FPAMPLSSLFANA	0.057	0.496	0.072	0.562	0.502	-0.994	0.496	0.304	P01244|SOMA_RAT	FPAMPLSSLFANA
FPAMPLSSLFANAVLR	0.104	0.552	0.036	0.488	0.621	-0.688	0.488	0.312	P01244|SOMA_RAT	FPAMPLSSLFANAVLR
FPAMPLSSLFANAVLRA	0.010	0.324	0.006	0.326	0.420	-1.250	0.324	0.490	P01244|SOMA_RAT	FPAMPLSSLFANAVLRA
FPAMPLSSLFANAVLRAQ	0.016	0.375	0.010	0.331	0.565	-0.824	0.331	0.480	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQ
FPAMPLSSLFANAVLRAQH	0.092	0.552	0.173	0.729	0.733	-0.448	0.552	0.258	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQH
FPAMPLSSLFANAVLRAQHLH	0.342	0.868	0.314	0.909	0.845	-0.243	0.868	0.062	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLH
FPAMPLSSLFANAVLRAQHLHQ	0.305	0.866	0.197	0.808	0.853	-0.230	0.808	0.092	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQ
FPAMPLSSLFANAVLRAQHLHQL	0.064	0.496	0.029	0.483	0.524	-0.932	0.483	0.316	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQL
FPAMPLSSLFANAVLRAQHLHQLA	0.049	0.496	0.024	0.440	0.541	-0.887	0.440	0.356	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQLA
FPAMPLSSLFANAVLRAQHLHQLAAD	0.004	0.305	0.006	0.326	0.427	-1.229	0.305	0.516	P01244|SOMA_RAT	FPAMPLSSLFANAVLRAQHLHQLAAD
FPKPEGSQD	0.091	0.552	0.314	0.909	0.753	-0.410	0.552	0.258	P47868|SCG3_RAT	FPKPEGSQD
FPKPEGSQDKSLHN	0.764	0.973	0.863	0.984	1.036	0.051	0.973	0.012	P47868|SCG3_RAT	FPKPEGSQDKSLHN
GPNLVARSKEALA	0.379	0.868	0.282	0.909	1.188	0.249	0.868	0.062	P02770|ALBU_RAT	GPNLVARSKEALA
GPVQFLSRIF	0.032	0.483	0.051	0.526	1.629	0.704	0.483	0.316	P01244|SOMA_RAT	GPVQFLSRIF
GPYRVEHF	0.956	0.979	1.000	1.000	1.048	0.068	0.979	0.009	P01194|COLI_RAT	GPYRVEHF
GPYRVEHFRWGNPPKD	0.738	0.973	0.426	0.932	1.271	0.346	0.932	0.031	P01194|COLI_RAT	GPYRVEHFRWGNPPKD
GRGREPGAYPALDSRQE	0.682	0.973	0.756	0.984	0.929	-0.107	0.973	0.012	O35314|SCG1_RAT	GRGREPGAYPALDSRQE
GRTLYGFGG	0.913	0.979	0.654	0.975	1.031	0.045	0.975	0.011	P62804|H4_RAT	GRTLYGFGG
GTQESVDSAKPRVY	0.059	0.496	0.132	0.637	0.839	-0.254	0.496	0.304	P01186|NEU2_RAT	GTQESVDSAKPRVY
GVASALAHKYH	0.732	0.973	0.705	0.984	1.067	0.093	0.973	0.012	P02091|HBB1_RAT:P11517|HBB2_RAT	GVASALAHKYH
IKMALQQEGFD	0.667	0.973	0.605	0.963	1.658	0.729	0.963	0.016	P28841|NEC2_RAT	IKMALQQEGFD
KYVMGHF	0.192	0.722	0.282	0.909	0.869	-0.202	0.722	0.141	P01194|COLI_RAT	KYVMGHF
KYVMGHFRWD	0.918	0.979	0.863	0.984	0.836	-0.258	0.979	0.009	P01194|COLI_RAT	KYVMGHFRWD
LDEGHDPV	0.060	0.496	0.099	0.622	0.759	-0.398	0.496	0.304	O35314|SCG1_RAT	LDEGHDPV
LDEGHDPVHESPV	0.740	0.973	0.918	0.984	1.136	0.184	0.973	0.012	O35314|SCG1_RAT	LDEGHDPVHESPV
LENSSPQAPA	0.312	0.866	0.349	0.909	0.853	-0.230	0.866	0.062	Q9QXU9|PCS1N_RAT	LENSSPQAPA
LENSSPQAPARRLLPP	0.690	0.973	0.756	0.984	0.977	-0.034	0.973	0.012	Q9QXU9|PCS1N_RAT	LENSSPQAPARRLLPP
LLDEGHDPVHESPVD	0.703	0.973	0.863	0.984	1.094	0.130	0.973	0.012	O35314|SCG1_RAT	LLDEGHDPVHESPVD
LLDEGHDPVHESPVDT	0.436	0.874	0.349	0.909	0.848	-0.238	0.874	0.058	O35314|SCG1_RAT	LLDEGHDPVHESPVDT
LLDEGHDPVHESPVDTA	0.852	0.979	0.654	0.975	1.012	0.017	0.975	0.011	O35314|SCG1_RAT	LLDEGHDPVHESPVDTA
LLRALASSNARAQQ	0.328	0.868	0.605	0.963	0.898	-0.156	0.868	0.062	Q792G6|GNAS3_RAT	LLRALASSNARAQQ
LPGLLLF	0.101	0.552	0.099	0.622	0.656	-0.607	0.552	0.258	P01230|LSHB_RAT	LPGLLLF
LPHLPGLLLF	0.083	0.552	0.085	0.622	0.547	-0.870	0.552	0.258	P01230|LSHB_RAT	LPHLPGLLLF
LVQLAGTQESVDSAKPRVY	0.719	0.973	0.512	0.963	1.082	0.114	0.963	0.016	P01186|NEU2_RAT	LVQLAGTQESVDSAKPRVY
MDQLAKELT	0.299	0.866	0.426	0.932	0.768	-0.380	0.866	0.062	P10354|CMGA_RAT	MDQLAKELT
MDQLAKELTAE	0.796	0.973	0.756	0.984	0.873	-0.197	0.973	0.012	P10354|CMGA_RAT	MDQLAKELTAE
MQIFVKTL	0.816	0.973	0.918	0.984	1.015	0.021	0.973	0.012	P62982|RS27A_RAT:P62986|RL40_RAT:P0CG51|UBB_RAT:Q63429|UBC_RAT	MQIFVKTL
NPYFDPLQWKNSDFE	0.690	0.973	0.605	0.963	0.866	-0.208	0.963	0.016	O35314|SCG1_RAT	NPYFDPLQWKNSDFE
NQEQAEQGREHL	0.533	0.885	0.705	0.984	0.736	-0.441	0.885	0.053	P10362|SCG2_RAT	NQEQAEQGREHL
PESAFSE	0.025	0.450	0.043	0.503	0.676	-0.565	0.450	0.347	P01179|NEU1_RAT	PESAFSE
PHFLYPK	0.477	0.874	0.387	0.932	0.889	-0.169	0.874	0.058	P05371|CLUS_RAT	PHFLYPK
PMFIVNTNVPRA	0.812	0.973	0.973	0.985	0.967	-0.048	0.973	0.012	P30904|MIF_RAT	PMFIVNTNVPRA
PVHESPVDTA	0.078	0.552	0.072	0.562	0.705	-0.504	0.552	0.258	O35314|SCG1_RAT	PVHESPVDTA
PVQFLSRIF	0.203	0.722	0.349	0.909	1.251	0.323	0.722	0.141	P01244|SOMA_RAT	PVQFLSRIF
Q(-17.03)ELGKLTGPSNQ	0.704	0.973	0.918	0.984	1.071	0.100	0.973	0.012	P10362|SCG2_RAT	Q(-17.03)ELGKLTGPSNQ
Q(-17.03)EPGAPAAGM	0.718	0.973	0.863	0.984	1.115	0.157	0.973	0.012	P15087|CBPE_RAT	Q(-17.03)EPGAPAAGM
Q(-17.03)EPGAPAAGM(+15.99)	0.804	0.973	0.756	0.984	1.030	0.042	0.973	0.012	P15087|CBPE_RAT	Q(-17.03)EPGAPAAGM(+15.99)
Q(-17.03)KIAEKFSQ	0.889	0.979	0.282	0.909	0.733	-0.449	0.909	0.041	O35314|SCG1_RAT	Q(-17.03)KIAEKFSQ
Q(-17.03)KIAEKFSQR(-.98)	0.471	0.874	0.557	0.963	0.936	-0.096	0.874	0.058	O35314|SCG1_RAT	Q(-17.03)KIAEKFSQR(-.98)
QAAFQKVVAGVASALAHKYH	0.212	0.740	0.223	0.871	1.480	0.565	0.740	0.131	P02091|HBB1_RAT:P11517|HBB2_RAT	QAAFQKVVAGVASALAHKYH
R(+42.01)PVKVYPNVAENESAEAFPLEF	0.361	0.868	0.387	0.932	0.627	-0.673	0.868	0.062	P01194|COLI_RAT	R(+42.01)PVKVYPNVAENESAEAFPLEF
RAEEETAGGDGRPEPSPRE(-.98)	0.604	0.952	0.756	0.984	0.695	-0.525	0.952	0.021	P01194|COLI_RAT	RAEEETAGGDGRPEPSPRE(-.98)
RPVKVYPNVAENE	0.890	0.979	0.918	0.984	1.051	0.071	0.979	0.009	P01194|COLI_RAT	RPVKVYPNVAENE
RPVKVYPNVAENES(+79.97)AEAFPLE	0.148	0.656	0.314	0.909	0.833	-0.263	0.656	0.183	P01194|COLI_RAT	RPVKVYPNVAENES(+79.97)AEAFPLE
RPVKVYPNVAENES(+79.97)AEAFPLEF	0.004	0.305	0.003	0.326	0.397	-1.331	0.305	0.516	P01194|COLI_RAT	RPVKVYPNVAENES(+79.97)AEAFPLEF
RPVKVYPNVAENESAEAFPLE	0.887	0.979	0.973	0.985	1.024	0.035	0.979	0.009	P01194|COLI_RAT	RPVKVYPNVAENESAEAFPLE
RPVKVYPNVAENESAEAFPLEF	0.367	0.868	0.314	0.909	0.893	-0.164	0.868	0.062	P01194|COLI_RAT	RPVKVYPNVAENESAEAFPLEF
RRPVKVYPNVAENESAEAFPLEF	0.904	0.979	0.809	0.984	0.992	-0.011	0.979	0.009	P01194|COLI_RAT	RRPVKVYPNVAENESAEAFPLEF
RTLYGFGG	0.367	0.868	0.349	0.909	0.845	-0.243	0.868	0.062	P62804|H4_RAT	RTLYGFGG
S(+42.01)MEHFRWGKPV(-.98)	0.236	0.773	0.314	0.909	0.840	-0.252	0.773	0.112	P01194|COLI_RAT	S(+42.01)MEHFRWGKPV(-.98)
S(+42.01)YSMEHF	0.489	0.876	0.809	0.984	1.009	0.013	0.876	0.057	P01194|COLI_RAT	S(+42.01)YSMEHF
S(+42.01)YSMEHFRWGKPV(-.98)	0.474	0.874	0.387	0.932	0.905	-0.144	0.874	0.058	P01194|COLI_RAT	S(+42.01)YSMEHFRWGKPV(-.98)
SAEAFPLEF	0.897	0.979	0.973	0.985	1.203	0.266	0.979	0.009	P01194|COLI_RAT	SAEAFPLEF
SAVISLEGKPL	0.623	0.973	0.557	0.963	1.053	0.075	0.963	0.016	P45592|COF1_RAT	SAVISLEGKPL
SEES(+79.97)QEKEY	0.289	0.866	0.387	0.932	0.786	-0.348	0.866	0.062	O35314|SCG1_RAT	SEES(+79.97)QEKEY
SLDKFLA	0.784	0.973	0.863	0.984	1.323	0.404	0.973	0.012	P01946|HBA_RAT	SLDKFLA
SLDKFLASVSTVLTSKYR	0.671	0.973	0.973	0.985	0.724	-0.467	0.973	0.012	P01946|HBA_RAT	SLDKFLASVSTVLTSKYR
SLKPEFVDIINAKQ	0.800	0.973	0.605	0.963	1.057	0.080	0.963	0.016	P48500|TPIS_RAT	SLKPEFVDIINAKQ
SLSAASAPLAETSTPL	0.430	0.874	0.099	0.622	0.886	-0.175	0.622	0.206	Q9QXU9|PCS1N_RAT	SLSAASAPLAETSTPL
SLSAASAPLAETSTPLRL	0.330	0.868	0.426	0.932	0.849	-0.236	0.868	0.062	Q9QXU9|PCS1N_RAT	SLSAASAPLAETSTPLRL
SMEHFRWGKPV(-.98)	0.402	0.868	0.605	0.963	1.380	0.464	0.868	0.062	P01194|COLI_RAT	SMEHFRWGKPV(-.98)
SNRVVDLMAYMASKE	0.413	0.868	0.468	0.959	1.226	0.294	0.868	0.062	P04797|G3P_RAT	SNRVVDLMAYMASKE
SPRIGQILK	0.255	0.805	0.349	0.909	1.334	0.415	0.805	0.094	P01244|SOMA_RAT	SPRIGQILK
SPRIGQILKQ	0.738	0.973	0.863	0.984	0.982	-0.027	0.973	0.012	P01244|SOMA_RAT	SPRIGQILKQ
SQENPNTYSEDLDV	0.200	0.722	0.426	0.932	0.839	-0.253	0.722	0.141	P06300|PDYN_RAT	SQENPNTYSEDLDV
SSEPEAAPAPRRL	0.022	0.443	0.036	0.488	0.682	-0.553	0.443	0.354	Q9QXU9|PCS1N_RAT	SSEPEAAPAPRRL
SVDSAKPRVY	0.094	0.552	0.223	0.871	0.591	-0.758	0.552	0.258	P01186|NEU2_RAT	SVDSAKPRVY
SVNPYLQ(-.98)	0.690	0.973	0.863	0.984	0.917	-0.124	0.973	0.012	P27682|7B2_RAT	SVNPYLQ(-.98)
SVNPYLQGKRLDNVV	0.891	0.979	1.000	1.000	0.941	-0.088	0.979	0.009	P27682|7B2_RAT	SVNPYLQGKRLDNVV
SVNPYLQGKRLDNVVA	0.103	0.552	0.132	0.637	1.170	0.227	0.552	0.258	P27682|7B2_RAT	SVNPYLQGKRLDNVVA
SVPHFSEEEKEPE	0.960	0.979	0.863	0.984	0.959	-0.061	0.979	0.009	P27682|7B2_RAT	SVPHFSEEEKEPE
SVSTVLTSKYR	0.928	0.979	0.918	0.984	0.897	-0.156	0.979	0.009	P01946|HBA_RAT	SVSTVLTSKYR
SYSMEHF	0.509	0.878	0.426	0.932	1.255	0.328	0.878	0.057	P01194|COLI_RAT	SYSMEHF
SYSMEHFRWGKPV(-.98)	0.869	0.979	0.918	0.984	1.098	0.135	0.979	0.009	P01194|COLI_RAT	SYSMEHFRWGKPV(-.98)
TAYAIYMLAMAAFPKKQN	0.553	0.907	0.809	0.984	0.986	-0.020	0.907	0.043	P35171|CX7A2_RAT	TAYAIYMLAMAAFPKKQN
TDMELLRF	0.858	0.979	0.973	0.985	0.973	-0.040	0.979	0.009	P01244|SOMA_RAT	TDMELLRF
TLYGFGG	0.231	0.773	0.152	0.673	0.860	-0.218	0.673	0.172	P62804|H4_RAT	TLYGFGG
TPAMHASLDKFLASVSTVLTSKYR	0.672	0.973	0.756	0.984	0.654	-0.613	0.973	0.012	P01946|HBA_RAT	TPAMHASLDKFLASVSTVLTSKYR
TPLPELFDRVVML	0.745	0.973	0.387	0.932	1.659	0.730	0.932	0.031	P01237|PRL_RAT	TPLPELFDRVVML
TPLVTLF	0.154	0.659	0.152	0.673	0.671	-0.575	0.659	0.181	P01194|COLI_RAT	TPLVTLF
TPLVTLFKNAIIKN	0.413	0.868	0.468	0.959	1.442	0.528	0.868	0.062	P01194|COLI_RAT	TPLVTLFKNAIIKN
TQESVDSAKPRVY	0.993	0.993	0.973	0.985	0.981	-0.028	0.985	0.007	P01186|NEU2_RAT	TQESVDSAKPRVY
VHLTDAEKA	0.951	0.979	0.863	0.984	1.140	0.189	0.979	0.009	P02091|HBB1_RAT:P11517|HBB2_RAT	VHLTDAEKA
VHLTDAEKAAVN	0.784	0.973	0.973	0.985	1.019	0.028	0.973	0.012	P02091|HBB1_RAT	VHLTDAEKAAVN
VHLTDAEKAAVNG	0.884	0.979	0.918	0.984	0.913	-0.131	0.979	0.009	P02091|HBB1_RAT	VHLTDAEKAAVNG
VKVGVNGF	0.507	0.878	0.605	0.963	1.147	0.198	0.878	0.057	P04797|G3P_RAT	VKVGVNGF
VKVGVNGFGRIG	0.441	0.874	0.654	0.975	1.178	0.236	0.874	0.058	P04797|G3P_RAT	VKVGVNGFGRIG
VPHFSEEEKEPE	0.458	0.874	0.512	0.963	1.095	0.130	0.874	0.058	P27682|7B2_RAT	VPHFSEEEKEPE
VQLAGTQESVDSAKP	0.877	0.979	0.605	0.963	0.923	-0.116	0.963	0.016	P01186|NEU2_RAT	VQLAGTQESVDSAKP
VQLAGTQESVDSAKPR	0.819	0.973	0.863	0.984	0.968	-0.048	0.973	0.012	P01186|NEU2_RAT	VQLAGTQESVDSAKPR
VQLAGTQESVDSAKPRV	0.112	0.572	0.173	0.729	0.698	-0.519	0.572	0.242	P01186|NEU2_RAT	VQLAGTQESVDSAKPRV
VQLAGTQESVDSAKPRVY	0.805	0.973	0.809	0.984	0.958	-0.062	0.973	0.012	P01186|NEU2_RAT	VQLAGTQESVDSAKPRVY
WSRMDQLAKELT	0.534	0.885	0.468	0.959	0.864	-0.211	0.885	0.053	P10354|CMGA_RAT	WSRMDQLAKELT
WSRMDQLAKELTAE	0.634	0.973	0.756	0.984	0.979	-0.031	0.973	0.012	P10354|CMGA_RAT	WSRMDQLAKELTAE
Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLF	0.014	0.375	0.020	0.440	0.472	-1.084	0.375	0.426	P01194|COLI_RAT	Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLF
Y(+42.01)GGFMTSEK	0.516	0.882	0.426	0.932	1.273	0.348	0.882	0.055	P01194|COLI_RAT	Y(+42.01)GGFMTSEK
Y(+42.01)GGFMTSEKSQ	0.962	0.979	0.863	0.984	1.158	0.212	0.979	0.009	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQ
Y(+42.01)GGFMTSEKSQTP	0.055	0.496	0.051	0.526	0.725	-0.463	0.496	0.304	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTP
Y(+42.01)GGFMTSEKSQTPL	0.118	0.587	0.114	0.626	0.709	-0.495	0.587	0.231	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPL
Y(+42.01)GGFMTSEKSQTPLV	0.448	0.874	0.654	0.975	0.850	-0.235	0.874	0.058	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLV
Y(+42.01)GGFMTSEKSQTPLVT	0.193	0.722	0.349	0.909	0.787	-0.345	0.722	0.141	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVT
Y(+42.01)GGFMTSEKSQTPLVTL	0.040	0.496	0.114	0.626	0.563	-0.828	0.496	0.304	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVTL
Y(+42.01)GGFMTSEKSQTPLVTLF	0.007	0.324	0.024	0.440	0.390	-1.360	0.324	0.490	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVTLF
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIK	0.102	0.552	0.132	0.637	0.704	-0.507	0.552	0.258	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIK
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKN	0.009	0.324	0.008	0.326	0.535	-0.903	0.324	0.490	P01194|COLI_RAT	Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKN
Y(+42.01)SMEHFRWGKPV(-.98)	0.480	0.874	0.809	0.984	0.809	-0.306	0.874	0.058	P01194|COLI_RAT	Y(+42.01)SMEHFRWGKPV(-.98)
YEENSRENPF	0.202	0.722	0.152	0.673	0.735	-0.444	0.673	0.172	P10362|SCG2_RAT	YEENSRENPF
YGGFLRKYP	0.425	0.874	0.605	0.963	0.917	-0.126	0.874	0.058	P06300|PDYN_RAT	YGGFLRKYP
YGGFLRKYPK	0.570	0.926	0.918	0.984	0.896	-0.159	0.926	0.033	P06300|PDYN_RAT	YGGFLRKYPK
YGGFLRRI	0.830	0.979	0.863	0.984	1.017	0.024	0.979	0.009	P06300|PDYN_RAT	YGGFLRRI
YGGFLRRQFKVVT	0.784	0.973	0.654	0.975	1.168	0.224	0.973	0.012	P06300|PDYN_RAT	YGGFLRRQFKVVT
YGGFMTSEKSQTPLVT	0.789	0.973	0.557	0.963	1.484	0.569	0.963	0.016	P01194|COLI_RAT	YGGFMTSEKSQTPLVT
YGGFMTSEKSQTPLVTL	0.145	0.656	0.314	0.909	0.733	-0.448	0.656	0.183	P01194|COLI_RAT	YGGFMTSEKSQTPLVTL
YGGFMTSEKSQTPLVTLF	0.379	0.868	0.349	0.909	0.794	-0.332	0.868	0.062	P01194|COLI_RAT	YGGFMTSEKSQTPLVTLF
YGGFMTSEKSQTPLVTLFKNAIIKN	0.767	0.973	0.557	0.963	1.209	0.273	0.963	0.016	P01194|COLI_RAT	YGGFMTSEKSQTPLVTLFKNAIIKN
YKVNEYQGPVAPSGGFFLFRPRN(-.98)	0.586	0.933	0.654	0.975	1.086	0.120	0.933	0.030	P12760|NMU_RAT	YKVNEYQGPVAPSGGFFLFRPRN(-.98)

Significant stat

Code

# get DE features only tibble
uni_res_filt <- uni_res %>%
        dplyr::filter(BHT < FDR & BHW < FDR) %>%
        mutate(status = case_when(`FC(log2)` < - 0.6 ~ "Down",
                                  `FC(log2)` > 0.6 ~ "Up") )

if (nrow(uni_res_filt) == 0){
        print(paste("There is no significant differentiated features between",
                    temp_d$Label[2], "and", temp_d$Label[1], sep = " "))
} else {
        uni_res_filt = uni_res_filt %>%
                left_join(., feature_meta, by = c("variable" = "Peptide"))
        
        #write.csv(uni_res_filt, file = paste(combo_list[[i]][1], "vs", combo_list[[i]][2], ".csv", sep = ""))
        uni_res_filt %>% 
                #mutate(across(-c(variable, status), round, digit=3)) %>%
                knitr::kable(., digits = 3, "html") %>%
                kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
                kableExtra::scroll_box(width = "100%", height = "500px")
}

[1] "There is no significant differentiated features between SP and No"

Volcano plot

Code

# volcano plot
volcano_plot(uni_res_annotation, feature_col = "variable", fdr = FDR, log2fc = LOG2FC, save = FALSE)

Heatmap

Code

# heatmap
anno <- data.frame(Label = as.factor(t(d5)[, "class"])) %>%
        dplyr::filter(Label %in% contrast ) %>%
        droplevels()

hm <- d5[-1, d5[1,] %in% contrast ] %>%
        rownames_to_column("variable") %>%
        mutate(across(-variable, as.numeric)) #%>%
        #left_join(., feature_meta, by = c("variable" = "Feature"))
        
heatmap(hm, feature_col = "variable", sample_anno = anno, feature_anno = feature_meta, feature_label = "Peptide", rowname_switch = TRUE, save = FALSE)

All group heatmaps

Ordered

Code

# for ordered column without clustering samples
col_order = c("No", "SP", "Iso")

# data wrangling for all group heatmap
norm_d2 = t(norm_d1) %>% as.data.frame()

norm_d3 = norm_d2 %>%
        arrange(factor(class, levels = col_order))

norm_d4 = t(norm_d3) %>% as.data.frame()
norm_d4_mod = norm_d4[-1,] %>%
        as.data.frame() %>%
        rownames_to_column(., var = "rowname") %>%
        mutate(across(-rowname, as.numeric)) %>%
        column_to_rownames(., var = "rowname")

anno <- data.frame(Label = as.factor(t(norm_d4)[, "class"]))
# levels() only change the name of each level, it doesn't reorder
# factor() to reorder factor level
anno$Label = factor(anno$Label, levels = col_order)
#levels(anno$Label)

rownames(norm_d4_mod) = feature_name$name

heatmap(norm_d4_mod, sample_anno = anno, sample_anno_col = "Label", feature_anno = feature_meta, feature_label = "Peptide", cluster_col_switch = FALSE, rowname_switch = TRUE, save = FALSE)

Clustered

Code

heatmap(norm_d4_mod, sample_anno = anno, sample_anno_col = "Label", feature_anno = feature_meta, feature_label = "Peptide", cluster_col_switch = TRUE, rowname_switch = TRUE, save = FALSE)

Reproducibility

The amount of time took to generate the report:

Code

Sys.time() - start_time

Time difference of 2.375097 mins

R session information:

Code

sessionInfo()

R version 4.2.1 (2022-06-23 ucrt)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 10 x64 (build 19045)

Matrix products: default

locale:
[1] LC_COLLATE=English_United States.utf8 
[2] LC_CTYPE=English_United States.utf8   
[3] LC_MONETARY=English_United States.utf8
[4] LC_NUMERIC=C                          
[5] LC_TIME=English_United States.utf8    

attached base packages:
[1] grid      stats     graphics  grDevices utils     datasets  methods  
[8] base     

other attached packages:
 [1] plotly_4.10.1       VennDiagram_1.7.3   futile.logger_1.4.3
 [4] wesanderson_0.3.6   RColorBrewer_1.1-3  extrafont_0.19     
 [7] pheatmap_1.0.12     ggrepel_0.9.3       ropls_1.28.2       
[10] ProteoMM_1.14.0     pROC_1.18.2         pcaMethods_1.88.0  
[13] Biobase_2.56.0      BiocGenerics_0.42.0 proBatch_1.11.0    
[16] cowplot_1.1.1       impute_1.70.0       statTarget_1.26.0  
[19] knitr_1.43.1        lubridate_1.9.2     forcats_1.0.0      
[22] stringr_1.5.0       dplyr_1.1.2         purrr_1.0.1        
[25] readr_2.1.4         tidyr_1.3.0         tibble_3.2.1       
[28] ggplot2_3.4.2       tidyverse_2.0.0    

loaded via a namespace (and not attached):
  [1] backports_1.4.1             Hmisc_5.1-0                
  [3] qqman_0.1.8                 corrplot_0.92              
  [5] systemfonts_1.0.4           plyr_1.8.8                 
  [7] lazyeval_0.2.2              splines_4.2.1              
  [9] BiocParallel_1.30.4         GenomeInfoDb_1.32.4        
 [11] sva_3.44.0                  digest_0.6.31              
 [13] foreach_1.5.2               htmltools_0.5.5            
 [15] viridis_0.6.3               GO.db_3.15.0               
 [17] fansi_1.0.4                 ggfortify_0.4.16           
 [19] magrittr_2.0.3              checkmate_2.2.0            
 [21] memoise_2.0.1               cluster_2.1.4              
 [23] doParallel_1.0.17           tzdb_0.4.0                 
 [25] limma_3.52.4                fastcluster_1.2.3          
 [27] Biostrings_2.64.1           annotate_1.74.0            
 [29] matrixStats_0.63.0          svglite_2.1.1              
 [31] extrafontdb_1.0             timechange_0.2.0           
 [33] pdist_1.2.1                 colorspace_2.1-0           
 [35] rvest_1.0.3                 blob_1.2.4                 
 [37] rrcov_1.7-2                 xfun_0.39                  
 [39] crayon_1.5.2                RCurl_1.98-1.12            
 [41] jsonlite_1.8.4              genefilter_1.78.0          
 [43] lme4_1.1-33                 survival_3.5-5             
 [45] iterators_1.0.14            glue_1.6.2                 
 [47] kableExtra_1.3.4            gtable_0.3.3               
 [49] zlibbioc_1.42.0             XVector_0.36.0             
 [51] webshot_0.5.4               DelayedArray_0.22.0        
 [53] Rttf2pt1_1.3.12             DEoptimR_1.0-13            
 [55] scales_1.2.1                futile.options_1.0.1       
 [57] vsn_3.64.0                  mvtnorm_1.1-3              
 [59] DBI_1.1.3                   edgeR_3.38.4               
 [61] Rcpp_1.0.10                 MultiDataSet_1.24.0        
 [63] viridisLite_0.4.2           xtable_1.8-4               
 [65] htmlTable_2.4.1             foreign_0.8-84             
 [67] bit_4.0.5                   preprocessCore_1.58.0      
 [69] Formula_1.2-5               stats4_4.2.1               
 [71] htmlwidgets_1.6.2           httr_1.4.6                 
 [73] calibrate_1.7.7             farver_2.1.1               
 [75] pkgconfig_2.0.3             XML_3.99-0.14              
 [77] nnet_7.3-19                 locfit_1.5-9.7             
 [79] utf8_1.2.3                  dynamicTreeCut_1.63-1      
 [81] labeling_0.4.2              tidyselect_1.2.0           
 [83] rlang_1.1.1                 reshape2_1.4.4             
 [85] AnnotationDbi_1.58.0        munsell_0.5.0              
 [87] tools_4.2.1                 cachem_1.0.8               
 [89] cli_3.6.1                   generics_0.1.3             
 [91] RSQLite_2.3.1               pls_2.8-2                  
 [93] evaluate_0.21               fastmap_1.1.1              
 [95] yaml_2.3.7                  bit64_4.0.5                
 [97] robustbase_0.95-1           randomForest_4.7-1.1       
 [99] ROC_1.72.0                  KEGGREST_1.36.3            
[101] nlme_3.1-162                formatR_1.14               
[103] xml2_1.3.4                  compiler_4.2.1             
[105] rstudioapi_0.14             png_0.1-8                  
[107] affyio_1.66.0               pcaPP_2.0-3                
[109] stringi_1.7.12              highr_0.10                 
[111] lattice_0.21-8              Matrix_1.5-4               
[113] nloptr_2.0.3                vctrs_0.6.2                
[115] pillar_1.9.0                lifecycle_1.0.3            
[117] BiocManager_1.30.20         pvca_1.36.0                
[119] data.table_1.14.8           bitops_1.0-7               
[121] GenomicRanges_1.48.0        R6_2.5.1                   
[123] affy_1.74.0                 gridExtra_2.3              
[125] IRanges_2.30.1              codetools_0.2-19           
[127] lambda.r_1.2.4              boot_1.3-28.1              
[129] MASS_7.3-60                 SummarizedExperiment_1.26.1
[131] withr_2.5.0                 S4Vectors_0.34.0           
[133] GenomeInfoDbData_1.2.8      MultiAssayExperiment_1.22.0
[135] mgcv_1.8-42                 parallel_4.2.1             
[137] hms_1.1.3                   rpart_4.1.19               
[139] minqa_1.2.5                 rmarkdown_2.21             
[141] MatrixGenerics_1.8.1        WGCNA_1.72-1               
[143] base64enc_0.1-3