Peptidomic Analysis Reveals Seasonal Neuropeptide and Peptide Hormone Changes in the Hypothalamus and Pituitary of a Hibernating Mammal

Author

Somayeh Mousavi, Haowen Qiu, Matthew T. Andrews & James W. Checco

Published

July 3, 2023

Doi
Abstract
During the winter, hibernating mammals undergo extreme changes in physiology, which allow them to survive several months without access to food. These animals enter a state of torpor, which is characterized by decreased metabolism, near-freezing body temperatures, and a dramatically reduced heart rate. The neurochemical basis of this regulation is largely unknown. Based on prior evidence suggesting that the peptide-rich hypothalamus plays critical roles in hibernation, we hypothesized that changes in specific cell–cell signaling peptides (neuropeptides and peptide hormones) underlie physiological changes during torpor/arousal cycles. To test this hypothesis, we used a mass spectrometry-based peptidomics approach to examine seasonal changes of endogenous peptides that occur in the hypothalamus and pituitary of a model hibernating mammal, the thirteen-lined ground squirrel (Ictidomys tridecemlineatus). In the pituitary, we observed changes in several distinct peptide hormones as animals prepared for torpor in October, exited torpor in March, and progressed from spring (March) to fall (August). In the hypothalamus, we observed an overall increase in neuropeptides in October (pre-torpor), a decrease as the animal entered torpor, and an increase in a subset of neuropeptides during normothermic interbout arousals. Notable changes were observed for feeding regulatory peptides, opioid peptides, and several peptides without well-established functions. Overall, our study provides critical insight into changes in endogenous peptides in the hypothalamus and pituitary during mammalian hibernation that were not available from transcriptomic measurements. Understanding the molecular basis of the hibernation phenotype may pave the way for future efforts to employ hibernation-like strategies for organ preservation, combating obesity, and treatment of stroke.

Project and data background

We hypothesized that changes in cell–cell signaling peptides from the hypothalamus and pituitary may be responsible for regulating critical physiological changes during the hibernation season. To test this hypothesis, we applied a non-targeted liquid chromatography–mass spectrometry (LC–MS) and LC-tandem mass spectrometry (LC–MS/MS) “peptidomics” approach to identify and quantify endogenous neuropeptides and peptide hormones as a function of season and hibernation stage in the thirteen-lined ground squirrel. LC–MS and LC–MS/MS-based peptidomics allow the detection, identification, and quantification of peptides without preselection, allowing for findings not possible with targeted antibody-based methods.

Thirteen-lined ground squirrel hibernation cycle. A. Active thirteen-lined ground squirrel. B. Torpid thirteen-lined ground squirrel. C. Overview of the annual hibernation cycle and timeline for tissue sampling. The central circle represents months of the year progressing clockwise, with the general active period in white and the hibernation states of torpor and IBA in gray, as indicated in the center. Colored outer regions and text boxes indicate time points for sample collection for this study. For time points in pre-hibernation and hibernation seasons, the measured range of internal body temperatures is indicated in parentheses.

Pituitary

Label-free peptidomics

Total number of samples after LC-MS: 37

  • August: 9

  • October: 9

  • IBA: 6

  • Torpor: 5

  • March: 8

Total number of feature provided: 124

Three step data processing:

  • Batch correction

  • Log2 transformation

  • EigenMS normalization

Batch correction using package statTarget evaluates the missing values and a feature will be kept if it has non-zero value for at least 50% of samples (statTarget default is 80%) in any one group (remove 31 features). It then imputes missing values for the present features and QC-based signal correction. At last, it removes features CV% > 50% (default, removes 3 features).

Code
pheno <- "CNS_meta4samples_pituitary.csv"
dfile = "CNS_data4samples_pituitary.csv"
#labels <- read.csv("CNS_label4samples_pituitary.csv", row.names = "SampleName")

statTarget::shiftCor(pheno, dfile,  QCspan = 0.25, Frule = 0.5,
                     degree = 2,imputeM = "KNN", ntree=500, coCV = 50)

statTarget: Signal Correction Start... Time: Mon Nov 20 19:24:07 2023 

* Step 1: Data File Checking Start..., Time:  Mon Nov 20 19:24:07 2023 

 Data Link 
 metaFile: CNS_meta4samples_pituitary.csv 
 profileFile: CNS_data4samples_pituitary.csv 

 44 Meta Samples vs 44 Profile samples
 The Meta samples list (*NA, missing data from the Profile File)

 [1] "20220302_QC1" "20220302_QC2" "Pituitary_53" "Pituitary_31" "Pituitary_38"
 [6] "Pituitary_63" "Pituitary_55" "Pituitary_51" "Pituitary_50" "Pituitary_34"
[11] "Pituitary_59" "Pituitary_43" "20220302_QC3" "Pituitary_66" "Pituitary_26"
[16] "Pituitary_19" "Pituitary_24" "Pituitary_41" "Pituitary_57" "Pituitary_47"
[21] "Pituitary_52" "Pituitary_23" "20220303_QC1" "Pituitary_58" "Pituitary_44"
[26] "Pituitary_37" "Pituitary_39" "Pituitary_46" "Pituitary_65" "Pituitary_56"
[31] "Pituitary_48" "Pituitary_35" "Pituitary_61" "20220304_QC1" "Pituitary_49"
[36] "Pituitary_60" "Pituitary_54" "Pituitary_45" "Pituitary_42" "Pituitary_62"
[41] "Pituitary_25" "Pituitary_64" "20220305_QC1" "20220305_QC2"

 Meta-information: 

    Class No.
1  August   9
2     IBA   6
3   March   8
4 October   9
5      QC   7
6  Torpor   5
  Batch No.
1     1  44

 Metabolic profile information: 

               no.
QC and samples  44
Metabolites    128

* Step 2: Evaluation of Missing Value... 

 The number of missing value before QC based signal correction:  732
 The number of filtered variables using the modified  50 % rule :   31 

* Step 3: Imputation start... 

 The imputation method was set at 'KNN'
 The number of missing value after imputation:  0
 Imputation Finished! 
 
* Step 4: QC-based Signal Correction Start... Time:  Mon Nov 20 19:24:07 2023 

 The Signal Correction method was set at QC-RFSC 

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 Calculation of CV distribution of raw peaks (QC)...

        CV<5%   CV<10%   CV<15%  CV<20%   CV<25%   CV<30%   CV<35%  CV<40%
Batch_1     0 3.092784 22.68041 37.1134 54.63918 60.82474 69.07216 74.2268
Total       0 3.092784 22.68041 37.1134 54.63918 60.82474 69.07216 74.2268
          CV<45%   CV<50%   CV<55%   CV<60%   CV<65%   CV<70% CV<75% CV<80%
Batch_1 77.31959 87.62887 91.75258 94.84536 95.87629 98.96907    100    100
Total   77.31959 87.62887 91.75258 94.84536 95.87629 98.96907    100    100
        CV<85% CV<90% CV<95% CV<100%
Batch_1    100    100    100     100
Total      100    100    100     100

 Calculation of CV distribution of corrected peaks (QC)...

           CV<5%   CV<10%   CV<15%   CV<20%   CV<25%  CV<30%   CV<35%   CV<40%
Batch_1 1.030928 20.61856 41.23711 59.79381 68.04124 74.2268 82.47423 90.72165
Total   1.030928 20.61856 41.23711 59.79381 68.04124 74.2268 82.47423 90.72165
          CV<45%   CV<50%   CV<55% CV<60% CV<65% CV<70% CV<75% CV<80% CV<85%
Batch_1 92.78351 96.90722 98.96907    100    100    100    100    100    100
Total   92.78351 96.90722 98.96907    100    100    100    100    100    100
        CV<90% CV<95% CV<100%
Batch_1    100    100     100
Total      100    100     100

* Step 5:  Removal of the features (CV% > 50%) Mon Nov 20 19:24:08 2023 

 No. of removed features: 3
 Feature name: LAGAPEPAEP KRLGVLFNPYYDPLQWKSSRFE TPEAQAAFQKVVAGVANALSHKYH 

 Output Link: C:/Users/qiuha/OneDrive/projects/MaxHub/projects/2023-07-03-CNS-groundsquirrel 

 Correction Finished! Time:  Mon Nov 20 19:24:08 2023 

 #################################### 
 # Software Version: statTarget 2.0 + # 
 # Email: luanhm@sustech.edu.cn# 
 #################################### 
Code
fh = read.csv(file = "statTarget/shiftCor/After_shiftCor/shift_sample_cor.csv",header = TRUE, row.names = 1, stringsAsFactors = FALSE, sep = ",") %>%
        t(.) %>%
        as.data.frame()
labels_d1 <- as.matrix(fh[1,])
N_feature = nrow(fh[-1,])

# change row annotation for heatmap
fh_mod = read.csv(file = "statTarget/shiftCor/After_shiftCor/shift_sample_cor_mod_pituitary.csv",header = TRUE, row.names = 1, stringsAsFactors = FALSE, sep = ",") %>%
        t(.) %>%
        as.data.frame()

feature_name = as.matrix(fh_mod[-1, "sample"]) %>%
        `rownames<-`(rownames(fh_mod)[-1]) %>%
        `colnames<-`("name") %>%
        as.data.frame() %>%
        rownames_to_column(., var = "variable")
  • Features = 94

Data preview (after batch correction)

Code
#rownames(fh)[-1] == feature_name$variable
rownames(fh)[-1] = feature_name$name
# display table
fh %>% 
        #mutate(across(-variable, round, digit=2)) %>%
        knitr::kable(., digits = 3, "html") %>%
        kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
        kableExtra::scroll_box(width = "100%", height = "500px")
Pituitary_53 Pituitary_31 Pituitary_38 Pituitary_63 Pituitary_55 Pituitary_51 Pituitary_50 Pituitary_34 Pituitary_59 Pituitary_43 Pituitary_66 Pituitary_26 Pituitary_19 Pituitary_24 Pituitary_41 Pituitary_57 Pituitary_47 Pituitary_52 Pituitary_23 Pituitary_58 Pituitary_44 Pituitary_37 Pituitary_39 Pituitary_46 Pituitary_65 Pituitary_56 Pituitary_48 Pituitary_35 Pituitary_61 Pituitary_49 Pituitary_60 Pituitary_54 Pituitary_45 Pituitary_42 Pituitary_62 Pituitary_25 Pituitary_64
class August Torpor IBA October August August August Torpor October March October Torpor Torpor Torpor March August March August IBA October March IBA IBA March October August March IBA October August October August March March October IBA October
AGAPEPAEPMELAKPSA 8440.891791 775.554963 894.443289 11.425360 1548.747707 16807.763410 7436.475845 24.346540 1116.529464 4772.051234 477.122095 1347.690917 4.423521 779.355400 5978.243266 14785.218490 1030.061559 1617.177966 963.767877 26.824983 4079.207738 1310.612505 1056.466008 1104.657872 109.499447 475.793453 1154.425233 1146.309064 952.829079 6014.112942 728.606820 6652.094453 40.168600 45.421386 133.549590 1696.183331 980.962340
LAGAPEPA 1637.378702 575.595032 975.630047 45.861858 618.251226 6120.353519 5723.721568 99.165909 48.814586 1434.796982 125.069634 8881.278769 163.538557 227.448079 872.068168 8555.658726 130.762483 1051.025765 15.254078 69.059286 2.970965 4940.096814 33.457682 40.529443 64.864598 2213.437898 82.727210 2029.214809 201.302627 5663.122020 55.439455 4929.551350 91.342434 73.612294 253.726328 5986.048232 218.966671
AFGPRGPGLQL 550.29629 649.65194 755.18678 345.80726 1221.06916 791.64920 881.68783 812.51054 20.84877 2777.31318 356.64258 456.75401 801.66424 688.52076 804.50951 548.14269 590.88412 333.61744 475.63361 624.53244 1184.96964 565.58643 362.17408 2421.97165 4249.72287 478.94991 1558.92600 1171.09202 2395.91454 1019.61708 2884.94196 2131.58046 1049.11926 453.65046 596.25238 180.22440 826.22788
AAMDLEL 525.06319 13.00213 874.75070 1218.98064 7439.16463 25.49921 297.86676 924.28122 689.19719 375.21130 629.74732 124.37370 784.78327 957.32957 2253.14606 671.64965 449.49625 303.24242 1481.36860 829.00897 611.15013 675.69158 736.71143 1012.66076 22.83013 952.48399 416.59759 57.17709 442.89607 552.91798 4101.33316 1482.06788 482.06852 814.28190 1113.41676 116.09223 137.89368
AELDQLLHY 1521.42903 1002.28099 319.04781 1904.33387 2971.66541 2247.60675 72.35338 1899.71042 2508.03824 4280.23291 3344.65898 858.16806 2704.74526 157.78970 796.25832 5154.79758 665.90566 331.78645 4495.39135 1127.25751 2517.78311 2150.77456 234.06884 3391.50560 1430.31875 360.19220 745.14195 244.52844 212.58989 1280.93174 706.79407 947.91799 2080.38023 1587.68654 613.10502 795.80551 718.56146
EDREEASLQDRQYASHHT 42.17504 797.29683 1235.93663 645.17823 184.07864 547.59149 489.51312 1215.11341 1156.37250 2059.00835 1077.36160 1069.12980 382.01498 980.73374 247.32133 586.13359 1602.37731 665.29413 1551.51409 172.45288 2739.57538 257.36413 1022.67087 2859.40863 697.95067 710.02483 364.97251 1410.20775 1408.38193 385.27649 767.71181 135.72669 703.49989 707.01072 632.72335 446.80660 519.89247
EDREEASLQDRQYASHHTTE 103.494268 527.248428 902.432299 530.758483 213.092060 538.638119 498.095470 977.873970 800.188114 1931.523871 531.038140 765.766642 414.387373 1069.247181 562.047648 645.154991 1606.570019 673.070093 1508.743502 235.332843 2955.934267 252.633601 1234.983948 4391.329206 679.960741 666.583467 662.772621 1163.094189 1411.795110 497.315730 1398.673698 307.393847 818.341676 207.556735 2.944484 534.580232 1040.775925
EEASLQDRQYASHHT 55.977706 577.709867 1326.664190 835.710334 55.430721 403.754716 396.416524 1575.441576 1555.499722 1816.940059 1266.284003 1069.694237 416.330001 941.406267 183.235430 222.398505 1248.631166 519.670512 1470.596814 252.783118 2282.001923 248.859735 1279.058948 2761.773044 1049.763295 208.921965 406.380606 1545.591591 1714.273409 102.133829 990.366681 71.689021 441.925256 110.825801 4.302888 505.348092 725.029992
EEASLQDRQYASHHTTE 139.71983 495.46860 1072.65147 572.76324 217.54310 527.15196 549.87897 1318.03658 940.23415 1734.41444 650.58705 787.96181 481.14419 988.25398 436.81115 623.64710 1790.77073 630.12132 1380.09498 267.59218 2384.29342 310.02315 1187.27888 3739.72553 1056.69198 580.88166 690.62213 1345.06364 2049.28193 368.99512 1596.60559 342.84747 566.92150 136.15521 25.30894 501.69637 1215.65739
EESEESEEGTTSEVT 254.28797 396.82302 1454.76710 524.26478 459.59644 223.76097 445.93341 2431.03205 803.17354 370.86905 1407.42960 397.53098 1538.47669 8100.06053 118.40360 403.21272 2990.34082 412.19670 2156.28479 1791.83580 2406.82879 323.59815 1303.54333 2270.37937 2172.79854 148.13757 283.37044 2946.79497 2340.76900 521.42110 2066.92534 424.30620 365.28014 65.94815 2086.61914 760.79018 987.37709
ELDQLLHY 1029.73311 1510.97697 1067.39391 669.81819 983.48923 628.20442 681.85894 1020.06360 1299.43517 1795.94991 1401.02161 460.30632 2154.10252 801.67790 1359.26169 856.45478 1468.28770 319.17416 1340.78192 788.31253 1803.85903 552.30906 790.46238 3595.49952 2729.17864 301.69341 2465.12908 1420.89800 1957.30849 97.86531 992.35921 417.34480 593.74461 32.96241 915.30199 194.77194 1188.81152
FLGEAYHHVPE 493.4311 322.7630 657.7509 369.3847 734.5341 629.2542 320.0933 677.0220 115.5980 2320.1347 374.6594 439.0358 949.0371 444.6169 1248.6644 1500.9906 300.2621 131.0724 1904.5890 1034.1041 670.1074 2005.1242 255.0434 1771.6900 1546.4188 127.9063 471.0338 291.5775 496.3285 612.4399 453.2683 162.8636 1206.0531 1271.5075 23.3822 1071.1865 326.5323
FLGEAYHHVPES 481.21481 654.24171 1047.65011 329.80621 842.32271 515.26592 682.86542 1326.22183 225.60144 2239.06766 721.41903 694.97339 1288.01876 504.01045 525.07038 528.91362 841.68651 1096.58151 1512.49735 1144.77095 954.72033 1152.73119 258.74070 1417.68697 1635.10268 72.67321 628.49496 643.30132 2065.45078 441.04958 787.10100 206.27630 957.96976 128.69579 213.47122 515.66261 285.22421
FLGEAYHHVPESQ 428.70360 758.26145 912.96618 406.40473 846.08793 79.05816 783.88383 1395.71003 249.02890 2313.96918 465.60644 809.88547 809.16139 749.01573 464.38649 554.87005 890.71067 299.68694 1294.83834 919.60470 841.72658 593.31631 185.36898 2804.12490 1713.15637 153.82720 1060.05182 1188.88783 2069.10972 84.42699 803.96634 495.43250 994.35460 367.69513 267.12389 564.01480 505.96548
FLGEAYHHVPESQRD 247.7085 488.8710 753.6601 482.4274 675.5019 372.5328 446.2955 883.9051 223.1465 2342.6750 325.4790 343.9048 700.3629 781.2719 1024.8392 527.7075 1813.8460 450.1383 1419.6284 851.5824 1643.1506 599.7167 362.1726 5741.6535 1112.5673 210.4716 2430.3198 963.9576 1414.9978 564.0439 1589.6277 677.4201 1246.0614 687.5427 20.1341 443.1950 530.4494
FLGEAYHHVPESQRDKA 63.83861 425.14360 708.01010 691.15817 345.96203 410.27961 421.94871 834.57931 232.34500 2402.56414 180.76880 548.61649 472.53191 820.97403 962.28249 745.17544 1806.78991 262.12347 1347.75389 681.88412 1137.75698 1544.45893 672.18398 5446.53105 645.23605 201.77124 1564.56968 609.19995 1340.20771 585.85442 2231.79484 614.44507 2304.55415 305.02820 949.50425 754.30831 762.21780
GEAYHHVPESQRD 108.5881 662.3954 1698.3930 352.3198 189.7030 106.8252 472.2040 1208.0028 446.6579 2028.1897 909.4631 782.0409 1480.9752 1081.6284 1589.6561 1685.8281 911.1899 160.9587 1582.7503 427.2400 1697.3173 1132.9635 1480.3471 3353.7126 987.9464 314.1462 894.3933 1224.2174 1607.2474 341.8750 2879.8256 530.0516 524.4536 330.8959 743.9444 876.1253 804.6718
GEAYHHVPESQRDKA 11.92236 395.62409 813.88227 501.06086 239.91843 400.09200 253.31832 734.02854 386.50858 1920.88127 174.43463 1008.40384 987.85891 684.55000 1872.89073 1242.10266 1235.12391 114.96755 1106.49652 428.55056 995.45208 2367.28765 2066.11269 4548.26675 720.33087 120.43822 805.45484 311.28189 1244.85174 432.56575 3258.74429 327.14693 1474.92964 251.63912 925.21924 457.54335 567.04523
GVLFNPYYDPLQWKSSRFE 6611.4346 875.9025 685.7050 472.3530 865.2550 1074.7683 3886.0569 331.1997 960.9155 7846.9287 543.5063 2637.2926 1005.2123 218.1399 1472.0790 6479.8435 1142.6181 203.2473 1050.6886 394.2170 1674.5674 2205.6678 960.0407 4358.9356 985.1988 1677.7208 2466.2344 881.2165 4798.2964 1318.4202 1715.2404 11978.9482 471.7387 2172.5147 1197.1231 1155.3646 1948.9287
LDQLLHY 275.9542 793.0110 895.8305 451.5764 1004.0843 279.7714 288.4017 1994.8197 932.9811 3206.3428 544.0862 219.0557 2486.7365 749.9878 1264.3156 621.4381 1052.6288 534.3379 1757.2866 1132.4615 2160.3010 1447.9382 865.8969 3079.2076 1420.9880 163.3341 1491.3648 724.8362 846.6669 262.0112 1123.4937 387.9258 1457.3647 1070.4640 1303.5664 455.8696 751.2653
LGEAYHHVPESQRD 167.21461 550.89299 761.28101 416.57572 664.14201 43.32686 192.79973 947.61855 127.53569 3021.89913 167.23181 190.43521 841.13448 878.00125 954.61050 721.53158 1938.28268 257.33678 1739.43515 878.69710 1862.44146 827.99034 184.08044 5794.52841 1276.08095 86.16034 2340.22125 730.11514 1150.07058 346.23813 1836.12807 822.88674 2173.45214 518.13479 738.44704 487.49897 39.91444
LGVLFNPYYDPLQWKSSRFE 8669.6495 627.6412 657.2180 2093.6500 4464.1618 2311.5561 10826.9857 1080.3823 1941.9735 9129.7677 1663.8509 2553.1065 417.4635 312.6697 442.5947 12800.4487 730.3036 806.9694 1341.2673 835.1249 3117.1786 2264.1320 176.6967 11557.5027 2057.3074 4560.8705 4188.6763 2091.2422 5271.7920 7873.1617 5597.6518 16651.9515 1866.7894 1832.6857 1731.6016 509.6936 1664.2611
Q(-17.03)KIAEKFSQ 394.65207 989.09485 1563.36256 174.54546 76.71573 631.76771 10.75832 2022.87482 332.67193 793.28098 239.85917 236.07875 396.79905 2330.69967 864.55441 241.44595 794.72469 581.56294 1167.23519 1036.85109 964.04812 412.62181 1028.77867 1979.95156 1382.01711 345.09604 1434.80009 298.36758 2180.02907 367.47702 2715.75645 74.14704 769.91394 125.13626 888.36737 313.74587 99.00356
Q(-17.03)KIAEKFSQR(-.98) 13.555279 412.189102 857.423054 63.616254 38.934850 530.182135 5.441377 1219.043920 366.004642 704.230341 77.965252 315.974786 418.366163 1271.987762 1415.228254 444.460457 1853.511233 230.219022 1428.640272 1358.385843 2389.541328 752.584749 1658.803085 3418.286575 1078.201212 706.032686 1213.248992 202.746358 896.524996 731.196235 3804.701044 120.987800 1791.039468 406.218462 311.066852 624.413222 103.382018
Q(-17.03)YDRVAELDQLLHY 16423.77064 220.46894 324.73862 3250.03108 3600.84988 3194.66819 13497.81109 854.97455 2641.47061 8033.12524 2085.40169 1450.06221 386.99210 271.41982 898.83112 15508.98794 849.99339 310.55343 840.38469 908.70988 2369.15980 2064.59586 46.19556 4049.32491 4933.21084 3718.76707 3083.31826 673.65307 3248.15809 5186.08688 4082.30745 33985.28329 3112.48130 4548.34755 95.67132 659.50459 567.47456
QYDRVAELDQLLHY 1779.169075 110.810896 1984.625763 3073.594982 4481.431768 1519.311376 2896.246777 1735.430427 2199.744258 3831.964478 3092.654594 548.393817 703.703883 504.263075 1197.232466 2186.854596 380.145695 372.932879 1400.544479 606.524804 4054.252621 1498.332456 383.825462 4.910719 2056.050241 1195.321366 578.218830 1101.266090 1118.642384 1406.298599 1456.922656 6738.520580 2090.537237 1501.578752 60.034898 425.761408 1175.979262
RVLTEEEEKELENL 1496.1039 1489.5749 1863.2634 425.9575 1257.1078 4316.7888 1761.4553 3408.0382 1564.9475 1699.1262 884.2519 553.1472 803.3353 3182.5054 2451.1502 1203.1286 362.0642 271.8096 5294.0775 4540.5105 1756.6278 1046.7054 1467.0762 1164.8802 1742.0443 1118.4210 1500.4058 1210.1720 3468.4995 2327.7286 10675.1755 408.1506 1036.4430 686.7855 126.4312 660.8108 225.2719
SQGGEPGAYLTPDT 933.8392 1007.3850 902.4255 354.2801 965.4500 302.4190 470.7034 875.1652 573.3923 1174.4731 320.0198 857.8360 878.6701 642.4561 959.2307 394.1289 1324.9163 692.6524 1408.6957 472.0319 937.2687 696.1563 500.3454 3518.6506 1927.9244 142.7497 391.3537 774.4224 1072.9653 410.5230 1002.5809 773.8843 627.6872 299.4090 568.1871 517.6635 1022.8996
SQGGEPGAYLTPDTREE 457.2856 436.5969 630.6995 330.7102 1144.8602 462.1013 501.0232 494.7017 579.0398 1321.0060 389.4226 565.9996 897.9201 861.9230 621.8995 617.8421 1222.0180 673.9228 1294.0884 1031.7024 769.4819 487.2670 506.1190 1807.9632 798.6428 182.9074 1293.4814 440.0699 730.5911 519.7184 907.3238 591.6321 688.3643 319.6933 894.5599 385.4963 774.6026
SSQEGNPPSEERGHTLQEPEEAKVGE 270.4985 730.6174 615.5500 436.6446 1081.0976 975.8356 1258.7788 1002.5869 569.9957 1896.9526 492.3425 1096.8643 638.1843 1216.7679 690.9403 1828.5668 1834.4817 762.8766 2071.3178 582.5196 2029.7862 853.9988 583.4784 3717.7003 2620.9343 788.7831 1875.7158 1705.9751 1000.8422 1656.7842 940.2866 1263.6121 2044.5158 190.9979 187.9178 1690.1970 929.7172
VAELDQLLHY 710.6329 1661.2074 995.6014 2024.5493 3404.2238 1685.2024 1091.5948 1560.6975 1872.9170 137.2283 1753.6776 503.8729 1332.1929 549.8334 1522.4090 1996.1246 540.6041 499.3167 2132.7675 989.0957 2696.9916 1340.7765 367.5124 5604.4396 1701.2064 644.0655 1015.1564 1223.6588 1170.9386 1159.5714 1486.2551 2652.1020 1270.3575 1139.8518 1164.6195 225.5111 539.7864
YDRVAELDQLLHY 1921.2349 779.4586 1183.3663 4642.7824 5231.8957 2200.0387 1612.0324 1169.5422 3437.6512 3720.9585 4187.5864 612.2748 571.2310 169.8196 2095.6228 8518.2837 193.6967 124.2603 2001.3383 1399.9847 4114.0251 3023.2594 116.8445 1047.7075 3320.1231 1317.3165 406.6363 125.3643 536.7377 3125.2496 1696.5310 7766.6467 3266.3952 4275.8072 1328.8320 866.0230 740.5959
Q(-17.03)EPGAPAAGM 1182.6064 576.3984 776.1936 614.1799 1189.9451 2163.6382 1409.6190 809.8199 537.4106 1427.3021 551.5436 765.6882 685.3389 691.1032 868.2769 1018.9770 1526.9570 1232.3924 1170.6636 871.0869 1397.0738 767.7241 608.4399 1653.6092 2759.6232 433.5372 148.7911 2045.6867 2017.5743 2077.3394 2125.9028 1808.6240 1003.3029 707.0681 1967.4411 649.9396 1036.2081
SLDSPAGPA 1407.60227 597.20486 899.90567 599.69070 876.35173 1004.43049 1218.87335 92.19355 1193.21654 744.44072 564.87617 814.56617 647.64301 167.62533 62.61696 1110.08840 4962.65211 2782.84682 60.08567 216.29559 1330.02735 729.76293 1297.97498 3689.57339 6127.75839 692.13749 6119.97577 5076.68455 1459.45084 451.28754 2567.89114 442.38150 1960.05130 56.60028 956.03057 2475.50683 3288.53589
SLDSPAGPAE 224.688672 776.741851 1139.862167 323.229867 785.439721 229.639017 1063.428607 110.943635 1542.458008 368.718257 434.823797 921.740524 389.621240 341.448954 39.152612 6.308664 5868.176667 2822.689795 6.518965 128.809849 779.917445 721.876138 1145.982574 3966.032611 4610.137183 168.784033 6080.818746 2725.968495 1275.506683 195.326074 1657.595108 164.686308 2047.773879 235.508453 5.756192 1898.797220 2854.147910
VKVGVNGF 2212.1589 879.3813 312.4563 1385.2188 4011.5349 2161.7012 354.8655 307.8283 2990.9895 735.4303 721.8823 1468.7655 541.6038 467.4043 2533.7810 728.5705 1485.0691 121.0391 897.2713 890.5916 1422.0956 1996.4345 787.9567 2124.6160 8452.9563 1242.5262 1293.7715 212.3951 314.7743 756.8240 293.1550 1231.0724 2685.2269 4635.4415 1491.9776 1439.5450 945.5468
Q(-17.03)HWSYGLRPG(-.98) 821.423755 887.395308 543.252364 573.822647 896.330038 156.486655 1322.969829 336.261309 271.589693 1232.051280 749.754688 55.270095 778.814142 349.856089 132.108752 89.240132 2185.141985 2174.950579 64.780758 326.457993 1046.780803 271.666059 7.128048 1345.333136 1344.761419 558.409425 2109.564757 1479.411528 2179.336941 608.336491 1081.103300 174.328387 1556.556669 994.782829 33.617020 544.336962 529.181934
SVSTVLTSKYR 511.74364 1066.83668 629.23461 630.64485 3291.97912 2792.32956 194.22572 502.86604 1242.77294 843.25636 742.65424 1058.82814 245.98463 877.25843 579.91026 329.10116 1844.81809 81.93880 1037.88314 2626.49562 815.30701 1796.57417 713.04349 1496.32049 2738.00545 385.33324 856.21166 73.93288 148.02640 1169.23286 597.59938 832.91949 1969.21699 1570.60247 1803.43038 1538.27855 1226.10399
GVANALSHKYH 84.97186 1420.81691 714.41442 1198.17922 1798.47488 473.04146 230.27930 859.93232 2164.51731 1565.97138 1204.75999 820.22776 218.86975 1253.04894 836.43356 1065.06708 4211.75240 83.51805 1722.24996 1795.43793 1781.37783 2311.58571 72.17162 2251.25115 2241.27363 1538.07529 61.26901 476.15935 116.28334 1298.83855 158.88758 911.07471 2812.79265 2195.76618 60.91228 19.43212 1171.83679
KVVAGVANALSHKYH 1295.3962 1228.5311 519.0045 855.6822 2369.9914 1028.9242 221.7337 476.4842 1179.8338 1920.9000 797.2765 925.3959 1419.1548 1188.2542 1135.3516 1072.8624 4026.0229 1482.9382 2048.9855 1439.2888 2041.0056 2698.7278 1232.3757 2661.1546 2109.3469 1183.9586 1319.4228 377.8791 1695.2464 2227.9271 1706.0109 1186.8453 3442.8773 2827.0645 1501.2990 1312.7818 968.7908
VANALSHKYH 67.77349 1510.54698 825.89552 2233.12137 901.70499 350.61214 348.92269 887.73587 4385.58244 1105.96771 2140.00307 690.91956 286.35218 1264.09210 840.82224 1769.60580 2323.03594 49.58380 894.60897 811.80865 1662.20278 3205.02220 57.44170 1938.65977 1856.28805 2007.90450 1601.26430 317.38474 53.90293 1569.80784 192.17078 1132.54046 1147.00757 1722.93609 64.90289 934.87208 1522.23353
GRTLYGFGG 210.79106 1047.61329 292.41303 1247.20614 2161.54613 2053.18357 245.41516 636.79697 1765.78190 168.56984 1112.69824 117.12027 732.53098 781.40538 1745.69030 395.69164 2406.19717 349.26655 1260.23134 1421.68460 1503.41041 1741.02772 127.54586 1569.82459 6212.41099 455.63762 262.09716 609.21820 587.59834 846.15612 400.16026 955.21457 3212.91745 1672.19179 1510.66823 96.54891 56.25776
RTLYGFGG 223.6174 978.2271 220.2125 1533.7512 2525.2230 1701.0789 174.2894 557.3945 2168.6592 301.2160 1100.3548 119.3319 641.3648 791.2343 1525.5741 331.1254 2565.5384 322.1529 1092.1647 1331.8070 1327.8681 1812.3511 191.5573 1191.3973 7186.2700 550.2879 283.0906 614.8341 626.6266 576.0633 562.7893 746.3481 3190.7879 2513.4609 1604.2847 148.7276 113.1286
TLYGFGG 1078.42158 2632.59413 1322.19361 2237.88478 4281.83058 2700.18873 3833.76464 228.52010 2696.80900 433.17355 2303.16981 36.58765 5557.08767 219.91817 1941.22982 825.04401 902.70937 3843.43252 449.70379 408.15663 505.09863 1527.71901 1161.16800 872.80191 5251.73941 509.61539 4088.48295 1983.02510 2220.11131 319.16476 991.16706 502.27567 1601.03847 3974.15696 375.33475 562.29194 1314.31080
AADQDLGPEAPPEGVLGA 2901.8157 672.7262 177.7539 358.4347 1313.7772 1262.3843 4857.9957 1052.5964 763.6487 3256.6101 755.3845 1450.1382 495.3376 116.4219 1540.4689 1655.2416 771.4656 174.7108 459.6791 491.2544 816.9146 1326.2971 518.6031 918.1944 1280.8434 1068.5097 2000.2317 1555.2968 840.4997 2130.0988 450.0622 3194.4151 1136.8468 230.8847 1079.4181 1843.1214 2223.0568
AADQDLGPEAPPEGVLGAL 20153.3480 956.6158 204.6107 6386.2001 8540.2739 3845.5771 22177.9979 2953.2585 3032.4969 15476.4061 2385.2506 2401.1125 164.0238 557.3158 2009.3125 9596.4939 827.6753 981.9257 997.2533 290.5575 3928.4518 1742.7590 1025.8917 5205.2513 8052.2519 6171.2306 3032.4938 1175.8996 2206.6297 8989.4532 939.2032 36212.0728 3388.6153 10166.8262 885.1045 2434.9761 2690.4065
LENPQPQAPARRLLPP 1190.76922 842.61542 439.98537 491.07796 930.34859 801.49427 1392.92750 188.72075 137.78095 977.42039 739.44097 977.16104 916.94428 422.38212 825.49809 780.67822 836.42948 1571.69028 262.91801 475.12640 781.69005 818.23824 206.25306 1274.53917 3190.16541 828.48681 1070.73824 1148.82049 1426.29262 2312.41875 1083.13822 781.48364 1992.09689 493.57180 34.18693 1305.72102 627.64834
VKMALQQEGFD 1433.4862 827.2746 407.0671 238.9144 2355.4541 5705.0479 2638.0575 629.5865 1136.9658 783.1829 257.5033 1726.0843 621.0280 334.7879 1295.9967 1052.0031 1172.2126 1299.0673 504.1724 721.1416 730.3526 1022.2065 388.7564 396.4622 3675.0981 1000.5473 842.4559 1938.2006 1100.3952 4473.3412 579.8407 1830.7952 1325.4213 370.3733 385.7423 880.4956 217.6216
GGFMRGL 1496.46263 1688.20867 591.83332 1498.25623 496.09793 177.63674 542.55803 1035.70028 934.90147 767.92367 1078.73139 1587.19706 5060.28470 90.51220 133.56343 253.05359 835.96457 2446.87459 656.92193 467.99198 792.96502 3460.92931 70.88582 1551.98913 4250.68241 589.34582 1106.41402 2006.17226 114.91533 648.92810 669.08974 883.41027 4533.29436 2048.69724 1950.28559 1273.68088 839.50276
Q(-17.03)LEDEAKELQ 775.99320 1479.36541 710.35774 459.31607 1647.03835 818.38893 161.52018 148.02224 306.23259 1303.45631 909.55371 1257.71787 1962.34358 213.01920 1692.68030 210.58887 2057.22799 4083.38718 177.80203 413.99111 1572.62460 1326.20146 1193.50844 1279.52295 5273.62608 767.82309 2320.95430 2982.26184 1476.29250 1064.10736 1366.63939 729.70625 1780.75972 578.13398 91.66786 1705.47619 4848.67783
SPQLEDE 276.940288 1396.146722 868.421715 1621.192868 541.077833 157.469711 1376.129368 173.778454 536.193917 599.786281 2129.672108 3.747196 2007.199859 182.917465 1630.921342 151.337529 2475.792186 3013.991442 168.921508 344.846822 977.798290 1594.029523 1164.211456 2069.255579 7458.458534 1465.719104 2979.703605 5069.150264 2789.936732 404.259081 1911.965961 407.764109 1594.143458 149.209407 50.698346 1416.144893 2577.922942
SPQLEDEAKEL 277.786221 1036.769599 654.393133 543.158332 1201.051478 532.904772 1153.984688 93.904069 227.779413 1256.263483 848.883078 11.723532 1718.711467 249.621916 232.122231 143.639516 2338.913313 3901.151115 230.170538 424.933721 1030.205023 1016.289722 9.938066 2206.949104 5638.847764 624.672814 1898.221903 1798.849490 1476.675831 906.315700 1807.089223 465.656026 2584.407912 542.809015 136.917168 1221.109312 2302.560668
SPQLEDEAKELQ 241.81852 927.04902 593.46943 412.70948 1069.11015 533.98925 801.28466 73.37816 214.17205 1128.25290 664.97569 858.80506 1447.58946 209.51772 185.81760 141.15500 1599.47307 2603.37298 284.19503 307.22308 614.62298 848.57225 844.03596 1204.66812 3106.45944 334.82910 1899.97455 1372.41877 1147.46899 452.55092 905.11587 234.48943 1240.27119 314.72025 158.90466 1060.96160 1903.28535
VGRPEWWMDYQ 1358.65238 1102.29997 889.81150 1231.63184 2599.58091 639.78954 1571.67682 247.10198 403.28590 1725.18970 2640.00610 44.69051 3200.06445 131.43542 129.04138 1423.58847 1377.74487 1899.69496 146.03149 528.95820 1174.19960 1615.34573 1051.83839 1615.29359 5583.56003 1107.01914 1930.71736 1734.28048 2425.28805 1193.97178 2159.53222 1164.79296 1945.54173 636.37644 864.14885 947.59896 1895.08407
YGGFM(+15.99)RF 308.72641 1857.00611 431.99899 1104.60674 3090.62302 1078.02620 1193.75619 144.04006 363.70816 499.25315 1458.25799 1265.20513 1295.66668 234.68305 162.85851 33.11038 2946.16832 2884.89510 343.08152 1159.78803 1007.22268 1484.29441 1485.36170 1133.40160 10249.66731 838.89131 3793.75036 4006.98441 4505.30649 534.95961 3558.76461 772.80113 2392.42032 373.12693 427.22210 1326.31802 2252.70854
YGGFM(+15.99)RGL 379.2099 2089.9072 731.6304 841.8555 2775.9645 374.2918 935.5794 83.4596 402.6122 1038.4256 1462.9972 1481.3787 1453.9900 210.7689 119.9386 1644.0987 3069.9812 3360.3432 374.7593 924.2952 817.3762 1649.4453 1548.2935 2332.4758 9752.8839 825.9417 3705.4005 4033.7214 3343.9755 635.2441 3495.1601 835.5263 3050.4199 329.4857 602.6551 2150.2488 2565.2627
YGGFMRF 497.01736 1072.45165 657.85941 390.74855 1517.08828 244.21196 1554.66620 112.81281 158.01486 1131.18459 946.43495 25.09619 2253.31242 114.42392 140.31986 66.76026 1874.88530 2053.41614 90.99779 339.80553 464.00538 570.87299 10.20850 2234.56324 5548.33404 626.14708 3144.52475 1639.39121 1949.17177 374.88962 1006.41738 379.70159 1174.01710 198.78394 20.12067 735.51331 1280.08874
YGGFMRGL 455.524465 1076.660757 731.799562 503.240530 885.183573 347.129756 1229.231896 64.953071 104.692304 976.237469 746.840484 13.584416 1732.230280 175.666854 166.674002 51.737575 1598.639631 2114.666742 142.632741 345.057006 1087.930780 1217.931336 9.304989 1577.833891 5638.117818 713.249485 2884.506078 1915.576318 1685.077620 825.545519 1258.356088 298.362835 1707.331613 515.077918 11.420737 723.209313 626.059157
EIGDEENSAKFPI(-.98) 684.13642 1410.96250 646.02682 302.45713 3245.14893 1194.65850 1627.76406 705.96206 829.37014 855.18367 660.46934 159.38188 1395.32122 824.27309 94.08887 212.89589 1681.87644 2244.52753 767.08280 420.17673 1534.93003 867.05916 72.27757 307.27640 2052.56475 814.77669 2291.23308 1853.18938 1223.98844 506.21449 744.46896 871.47618 2913.11112 347.84685 1713.70450 1711.82257 1297.38821
DDGPYRMEHFRWGSPPKD 888.0572 963.9873 778.7072 159.2630 1088.9537 3044.2984 2095.5458 682.8386 701.4690 7495.9115 412.2031 1752.1296 526.3064 357.5323 2394.6282 1438.4015 767.3897 365.3827 805.7882 745.8070 1334.7368 2395.1000 501.5079 666.1333 389.5712 1328.1925 523.2651 1088.8715 488.6027 8686.6042 227.8335 1777.8577 1591.5709 364.4288 1053.8615 1302.8425 157.8620
FKNAIIKNAH 268.18564 2262.00275 630.90863 222.67547 459.62972 538.33252 2842.81733 1521.00816 2097.98308 1872.80321 741.44975 1235.61020 431.51476 654.35859 2376.64313 2678.26587 2196.37466 19.90206 545.49302 683.86008 834.08123 1213.78134 1969.22179 1076.24002 1284.69057 2033.28923 1179.15512 2507.17002 451.98455 4786.01655 923.92415 1309.30975 1885.42008 263.12043 984.08458 1817.17792 1008.37974
FKNAIIKNAHKKGQ 1068.24973 616.22640 565.04669 360.62832 346.00555 77.54253 1326.01402 1852.36027 1833.90254 978.62750 4537.85561 261.39455 696.48980 1427.84678 943.04308 4570.06188 147.62783 671.19572 990.27794 849.31405 2653.58271 635.24536 2415.98368 1810.29062 228.90026 1771.95713 26.62369 458.20485 426.99343 1281.29942 287.09892 904.95755 90.10210 262.74748 463.27945 475.99949 47.21013
GGPEFRDDGAEPGPRE(-.98) 184.34248 409.66628 516.77914 838.62365 2540.15618 1655.25502 222.58258 519.13462 1381.73306 1554.81341 575.42936 220.52535 163.16835 447.08426 1992.40359 1841.38729 1067.70061 162.55123 1192.38880 3808.54640 3507.88639 1130.17613 72.16027 1693.86708 3408.05571 1422.56663 352.24162 255.41762 534.41627 1162.01400 350.11235 604.42017 1723.70927 184.02595 257.81121 102.00221 117.24573
GPEFRDDGAEPGPRE(-.98) 230.43919 491.78614 586.33114 1742.38708 2758.03902 1447.44269 239.64908 486.38715 1509.67262 1083.83336 1165.46144 161.95845 157.08193 404.09396 1897.64600 2055.16359 1128.97156 165.90879 1231.57062 1824.44855 4294.58862 1313.28439 92.07857 1940.39295 3481.14670 1865.25933 422.14152 306.39618 610.68631 943.73315 372.71368 598.73971 1605.97972 1744.80208 475.44030 162.96999 144.46238
KYVMGHFRWD 666.83001 919.03956 488.17867 79.26367 2276.84373 3537.79734 1259.29414 891.59384 820.76288 1853.21539 132.03477 1190.99449 216.61804 316.29118 1862.93105 847.15950 796.49801 365.55210 476.94614 1044.53757 942.44799 1692.66198 824.08453 257.70230 646.71090 908.59956 335.65493 1020.51415 171.39937 3264.04992 495.70035 1228.04311 1074.60414 287.91871 799.49838 1156.08301 545.24816
KYVMGHFRWDRF(-.98) 523.94947 610.84660 208.12384 103.28573 1553.99133 3179.39383 1145.10540 690.21057 787.75085 1489.83337 220.14246 1178.58549 222.54323 231.15933 2435.86516 1234.28548 847.97340 347.19952 568.89702 447.92820 1414.73048 1804.43796 604.92168 638.78623 195.89304 1100.33812 520.56015 443.48029 291.18103 5564.39222 135.57800 1092.37310 2722.20930 357.51755 1007.42049 1317.40521 80.29325
PEFRDDGAEPGPRE(-.98) 101.5571 596.9674 452.4494 1990.4450 2191.1090 1305.3927 318.2228 753.1191 1962.0430 1404.0559 709.4040 205.6463 160.8840 479.5994 1850.9203 1693.1350 1543.1788 225.1386 1603.9442 1974.4034 4349.6843 1117.5126 888.3964 1639.6116 2423.2787 1500.7441 666.3537 185.0070 875.8161 802.2767 332.5011 340.0470 1188.1347 1039.8707 188.8059 242.9633 173.2729
RPVKVYPNGAEDES(+79.97)AESFPLEF 11070.911012 1024.212935 598.930738 803.337809 1890.399514 5483.960323 12819.912157 1280.582473 2013.762122 2.263158 985.079174 985.226118 177.458969 149.420336 1288.865876 11322.215087 137.203384 350.236262 452.653906 1318.013793 525.399139 1313.939201 89.573796 398.426466 4241.817379 1952.205252 655.557869 1316.604545 1001.470239 4223.601885 559.535447 8715.438528 575.801552 411.634553 98.938620 314.634411 853.556459
RPVKVYPNGAEDESAESFPLEF 8413.8285 413.0344 338.3428 661.9594 3161.6411 11184.3257 3404.5756 2516.1064 1870.7808 2733.8307 998.1490 2845.4641 510.6685 106.7193 3105.8971 2646.3593 181.0622 109.3660 690.8507 1919.2375 442.4930 311.2595 120.4821 1401.7923 1986.6106 585.1426 425.6389 859.3504 318.6119 1767.5116 420.8891 7855.0619 150.8136 317.5392 130.3227 436.2797 860.9705
S(+42.01)YSMEHFRWGKPV(-.98) 1445.26051 1034.17101 594.73437 216.16841 1849.27024 5106.88113 2373.08811 762.46943 751.09516 3995.77067 222.74034 1434.68463 221.32407 273.21215 2159.03240 1318.43543 561.81899 201.60802 621.99264 1744.30736 867.82676 1931.20450 354.59992 1123.50072 1586.33718 822.79776 772.28777 1137.27354 556.57733 6273.38074 212.44451 3022.66171 2174.82932 304.06473 20.09392 1107.82212 42.29011
SQTPLVTLF 2026.3571 1338.3774 1623.4480 2445.3891 2433.8217 2375.7449 1749.4797 2302.1809 958.0414 1496.5210 1965.0195 509.4767 966.5813 992.6191 1519.5531 921.3968 731.3100 782.0178 1802.1732 1235.6303 1699.5273 241.5109 1093.2346 676.9668 3519.4247 369.1602 3336.4293 1910.7103 1857.1677 1348.9777 1192.4735 2850.4817 440.4337 347.0073 3960.2972 18.1852 1008.0168
SQTPLVTLFKNAIIKNAHKKGQ 117.48860 757.42141 280.49213 1837.08784 1090.60960 1926.86155 1054.30282 550.62580 1280.11986 26577.13347 2518.14077 162.30858 95.95411 35.02182 219.53943 1019.87965 1126.77236 786.73696 1416.79001 893.44103 2665.32701 550.16239 871.62217 4885.21030 790.21630 515.94608 1189.60516 972.49127 1543.78200 430.22349 235.00719 1618.50745 722.11728 549.73388 525.34170 826.92977 1804.46577
SYSMEHF 251.8199 454.5423 1294.8463 720.5086 1186.6389 1207.4403 477.5258 1406.0815 662.9903 1143.6044 873.6375 595.1338 544.0245 1035.8847 1347.5163 388.6288 1444.8139 1321.3567 1109.8358 1271.2487 1666.5523 596.2820 1446.0838 1652.4600 1869.7001 247.4647 752.6563 1594.6875 1185.4428 772.3699 1695.7522 1509.0425 687.0539 1371.3856 2411.2158 385.8057 1447.4501
SYSMEHFRWGKPV(-.98) 871.230465 500.403496 643.408852 162.443722 1217.779121 1200.968282 792.456632 678.602887 282.251955 4061.782860 364.162704 1137.518104 442.042424 373.295725 1345.266997 1461.102210 1093.807251 862.250912 1337.728993 685.261637 2349.972008 1047.818175 392.938806 1006.289154 482.209703 482.141417 581.026236 416.758618 566.741233 899.759385 377.526805 602.579019 621.020594 1414.679349 4.007785 846.135939 735.753967
TPLVTLFKNAIIKNAHKKGQ 39.017319 59.253908 324.591693 5997.692736 4015.516224 1727.033128 265.104454 59.435508 2515.269223 1015.082744 614.686639 1326.689093 582.734372 51.543614 2577.540941 3281.249169 145.988688 5.532145 599.201862 606.678534 2751.414666 911.412250 708.273628 2352.920690 1273.930689 3405.909709 130.065672 115.108949 123.705923 592.024646 125.156847 1279.954375 1360.806783 8028.490536 1589.838004 1710.198870 951.774009
Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLFKNAIIKNAH 14162.883902 2365.057731 158.584889 150.033360 1631.640545 9011.993295 14121.987855 459.118373 2756.309415 2474.392467 375.559132 1208.715658 1036.564495 749.624185 2058.697338 2656.479389 668.447605 511.980078 519.999158 855.164940 436.039162 3514.722470 158.205674 895.194346 3636.670696 2895.218123 2333.079053 2940.203022 744.520437 5817.330372 325.354293 13247.741149 1609.137381 196.111785 8.994823 2012.047551 640.937726
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNA 23585.955975 382.829697 561.830452 2.812048 3284.611173 19705.363557 23676.182460 387.076220 1236.833564 5527.907107 443.781787 2161.675193 54.842816 484.070825 288.411734 6380.381734 136.686925 162.916960 133.572199 430.824586 148.733429 1542.313664 560.927937 437.555832 2188.827943 2285.938157 586.843613 1073.933933 324.560096 10578.781438 137.602105 21344.819396 1093.705647 135.836551 865.835574 1553.389076 501.464470
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAH 10562.507025 1313.873053 326.827403 185.917882 1569.881824 6231.741689 11887.322857 875.327942 1576.701996 4496.915948 369.626719 1895.314932 116.868448 128.287745 1991.375605 3063.330306 588.820515 322.615369 504.834166 624.502360 931.560661 3005.680615 318.987323 1286.978004 3566.243306 3087.327742 2298.889006 3011.368614 702.229602 12637.042044 197.312580 8671.848646 1288.368399 153.314102 1.266135 1189.672749 92.427463
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAHKKGQ 1665.118109 2358.463974 462.164207 323.549390 124.093425 2876.484699 1764.176098 1967.862107 737.734284 3099.549506 918.660769 536.229356 273.353854 411.823698 2090.703456 994.902695 458.077857 652.567267 1210.835641 229.356185 1213.990431 2246.736673 807.261405 565.522042 624.540004 756.459013 1449.365160 919.152149 290.393644 5464.374704 324.698935 3339.414959 716.832285 137.239539 5.673444 1017.026609 257.101993
YGGFM(+15.99)TSEKSQTPLVTLFKNAIIKNAHKKGQ 855.61804 1564.62538 679.22428 3299.93371 988.48249 419.93398 1529.47214 1928.35277 1212.12873 2146.84750 3696.69073 189.13095 111.49999 1318.82335 375.64435 1651.57970 2915.53495 2794.01381 2534.75999 887.30389 1905.95113 297.58898 1149.79025 5203.24189 555.50651 1204.74838 6925.62624 2044.63784 3871.58467 346.47124 1266.41939 1445.10787 11.67290 590.22729 86.98747 465.23928 6528.60896
YGGFMTSE 698.4797 461.6090 926.2728 579.2999 1302.5862 1772.6989 726.7832 1327.9245 470.9490 4159.3878 559.5050 505.2308 551.4393 1257.9355 1113.2142 752.3002 1126.7745 1627.2060 1244.2337 840.4334 1524.7202 771.6526 1253.4835 1401.1738 2089.1147 255.5741 2800.7054 1989.6387 1203.5297 1502.7101 1267.6343 3296.1996 546.6067 937.7522 2665.3345 332.3717 1065.0374
YGGFMTSEKSQTPLVTLFKNAIIKNA 2951.5013 1539.9506 646.0024 689.7253 3335.0018 6548.3851 3512.8243 1398.3273 1727.1455 6010.2293 826.5009 2729.5885 184.4494 250.1372 1686.4381 7816.5240 817.2334 1608.1524 1620.6477 794.3135 2752.2311 3901.2476 426.4441 1762.2194 11793.6100 2887.3495 2554.2672 2294.5725 3100.5964 5287.8632 2175.2867 6908.9752 1365.0428 1473.6719 2038.7831 2393.6143 1054.3216
YGGFMTSEKSQTPLVTLFKNAIIKNAH 873.61538 2534.52617 1003.51082 474.94346 2619.84130 3612.68479 997.01071 1014.50509 686.67347 1824.02489 219.21242 904.18906 904.63712 304.38916 2327.82683 4316.03166 86.29545 606.95202 709.87681 444.40690 1193.59729 2882.42940 574.67644 201.99292 4608.70178 1080.22942 531.03424 2120.79391 1045.03194 2485.87129 730.94920 3774.92429 530.61881 405.83311 1057.42788 2154.77732 503.54405
YGGFMTSEKSQTPLVTLFKNAIIKNAHKKGQ 393.11266 830.16951 900.89913 1823.70062 969.88503 515.22282 1392.10677 2702.47749 2313.65046 3007.59031 3636.37601 605.44693 861.46873 863.07440 729.28256 820.22952 1335.47654 998.89814 1162.14875 437.77553 2709.20596 369.74234 1043.47270 3537.03313 310.27799 734.82812 3108.06961 817.92246 1168.32549 368.95374 819.46657 998.11605 331.29404 184.76103 60.03794 342.45879 2596.88959
ATLESVFQELGKLTGPSSQKRE 3294.04044 903.90235 935.07711 1895.27679 777.60628 2856.17627 1788.73418 959.81735 1316.80474 3300.47857 2771.12015 2015.65181 2279.14060 416.85423 2890.40177 1192.75292 1197.34697 2522.08608 5283.46813 1174.28619 1925.02997 2127.06071 1720.28421 4274.33010 1432.29168 191.30353 2295.86514 96.88988 2327.35350 216.47887 7822.12955 542.79891 430.69764 707.59705 721.83937 727.12987 1677.23823
MYEENSRDNPF 305.1265 567.2840 946.3929 379.4790 776.3466 847.9241 598.9437 973.3149 1012.5519 1420.9099 547.8667 565.5895 981.6504 751.4806 837.0393 311.9473 1301.8650 708.3824 1629.0183 609.6255 1354.0874 727.9994 1024.1684 1403.1404 2867.3674 196.5511 650.2069 1118.6491 2479.7351 434.4935 3143.0369 874.4292 525.1122 656.3637 332.9595 307.8804 417.7585
TNEIVEEQYTPQSL 2314.7539 1336.9040 3433.1293 425.7653 1255.2410 3517.1165 290.5042 1436.5918 2870.4362 3490.4357 279.9286 417.9799 1378.7863 1181.1678 2541.7544 2009.4243 703.1639 42.0380 5958.2769 3529.5288 1472.9574 1687.4461 2177.8225 3152.0782 3019.2665 656.9153 3505.1688 275.2392 4599.9721 751.0174 13376.8058 1084.1515 969.0547 677.7245 2963.3904 777.1403 136.0975
FPTLGGSQDKSLHN 664.2153 370.2542 557.7496 221.4898 597.3154 606.4768 371.3213 588.3091 372.4494 1565.7994 345.6432 698.4697 672.1565 578.0652 1128.3113 509.1754 1963.2686 864.1597 720.0531 927.4844 1430.8851 873.7425 926.7753 1476.3045 2076.4818 389.9772 2021.4806 802.8369 1225.4325 954.9039 1629.4122 434.4113 1162.6729 435.7855 1296.2335 571.3601 589.7155
TLGGSQDKSLHN 9.40251 592.52172 802.17080 561.50195 115.96206 159.53973 349.82833 1054.58435 2160.66247 1168.83266 714.69657 360.78445 885.07056 850.57742 1887.46821 1041.36511 2003.38339 294.67852 547.65671 370.16717 3213.77105 889.56429 2026.84314 2339.60508 1338.58510 596.74598 1144.12295 602.37438 1464.63799 323.35547 1665.69287 228.59093 710.10797 443.24938 434.63305 51.13244 390.57529
SVNPYLQGQRL 485.96282 411.30195 733.85188 38.58792 86.95171 817.31076 22.67851 1159.42085 513.10949 496.82094 53.22789 370.91560 607.23846 808.71197 2386.96739 196.65309 1552.83320 125.40403 1046.38220 829.46815 1821.59823 529.74321 1004.27342 1352.80673 1174.56028 277.67287 1059.35390 145.33931 1015.85492 439.73768 3538.90011 79.60038 884.72503 1232.92605 1142.59679 288.76396 30.83659
SVNPYLQGQRLDNVV 3815.52145 610.10195 851.37226 2049.88684 2490.87854 6634.58109 3752.73163 751.16707 1720.10395 2761.64171 1618.63698 1535.62826 17.36857 405.98752 774.78039 3009.91874 429.25437 506.77740 1262.37252 1164.57054 2597.84915 685.81527 242.01545 2403.89090 4948.88733 2261.88994 2771.05251 1594.16713 2287.64083 4374.91678 991.23702 5924.71792 728.08695 1855.16075 1542.86184 609.48502 624.73813
SVNPYLQGQRLDNVVA 2694.0518 647.5007 673.2173 1477.8779 2094.4568 3090.5216 2525.7932 720.1189 1055.1988 3077.7936 1142.0299 1543.8883 530.1360 405.8454 1761.6106 1651.9937 1074.6270 621.1074 1130.0799 913.4880 3208.9678 978.3610 404.5577 3778.7130 3976.7559 1346.4645 2928.0116 1292.0485 2099.3698 3167.2281 1268.3325 3335.1360 1377.1888 1314.7793 1541.2214 341.3430 602.0742
SANSNPAM(+15.99)APRE 1260.112708 2407.584605 1322.967798 1138.071781 781.401032 21.081017 916.543676 30.822463 568.511184 688.646946 1801.814999 1498.654531 958.876436 45.279093 5.431026 35.691451 4102.192382 2906.255234 270.550403 162.012907 1802.270129 1201.215803 1611.176928 4154.590623 3845.167459 946.164790 1575.399704 2388.857335 6502.607984 125.496761 3124.517422 31.557790 2130.711937 297.813342 1347.675117 2533.847678 1858.003876
SANSNPAMAPRE 216.84933 1064.92141 1023.03393 632.29622 633.01556 56.89700 1342.72703 64.95270 324.50022 1817.11808 731.77210 32.48293 844.89077 48.98649 187.64042 84.60936 3282.09110 1949.56949 99.86112 430.44818 1768.89111 620.53899 15.36907 3199.86188 3633.86203 1038.03566 2087.23858 3026.89867 2434.76128 309.08295 1282.94845 86.84305 1642.58491 474.95798 126.71698 834.54089 1060.56441

Data transformation applies a mathematical transformation on individual values themselves. For mass spec data, log transformation is a good choice, as it reduces or removes the skewness of mass spec data.

Code
plot_grid(hist_log2, qq_log2, pca_log2, nrow = 1)

Code
#rownames(log2_d1) == feature_name$variable
rownames(log2_d1) = feature_name$name
# display table
log2_d1 %>% 
        mutate(across(everything(), round, digit=2)) %>%        
        knitr::kable(., digits = 3, "html") %>%
        kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
        kableExtra::scroll_box(width = "100%", height = "500px")
Pituitary_53 Pituitary_31 Pituitary_38 Pituitary_63 Pituitary_55 Pituitary_51 Pituitary_50 Pituitary_34 Pituitary_59 Pituitary_43 Pituitary_66 Pituitary_26 Pituitary_19 Pituitary_24 Pituitary_41 Pituitary_57 Pituitary_47 Pituitary_52 Pituitary_23 Pituitary_58 Pituitary_44 Pituitary_37 Pituitary_39 Pituitary_46 Pituitary_65 Pituitary_56 Pituitary_48 Pituitary_35 Pituitary_61 Pituitary_49 Pituitary_60 Pituitary_54 Pituitary_45 Pituitary_42 Pituitary_62 Pituitary_25 Pituitary_64
AGAPEPAEPMELAKPSA 13.04 9.60 9.80 3.51 10.60 14.04 12.86 4.61 10.12 12.22 8.90 10.40 2.15 9.61 12.55 13.85 10.01 10.66 9.91 4.75 11.99 10.36 10.05 10.11 6.77 8.89 10.17 10.16 9.90 12.55 9.51 12.70 5.33 5.51 7.06 10.73 9.94
LAGAPEPA 10.68 9.17 9.93 5.52 9.27 12.58 12.48 6.63 5.61 10.49 6.97 13.12 7.35 7.83 9.77 13.06 7.03 10.04 3.93 6.11 1.57 12.27 5.06 5.34 6.02 11.11 6.37 10.99 7.65 12.47 5.79 12.27 6.51 6.20 7.99 12.55 7.77
AFGPRGPGLQL 9.10 9.34 9.56 8.43 10.25 9.63 9.78 9.67 4.38 11.44 8.48 8.84 9.65 9.43 9.65 9.10 9.21 8.38 8.89 9.29 10.21 9.14 8.50 11.24 12.05 8.90 10.61 10.19 11.23 9.99 11.49 11.06 10.03 8.83 9.22 7.49 9.69
AAMDLEL 9.04 3.70 9.77 10.25 12.86 4.67 8.22 9.85 9.43 8.55 9.30 6.96 9.62 9.90 11.14 9.39 8.81 8.24 10.53 9.70 9.26 9.40 9.52 9.98 4.51 9.90 8.70 5.84 8.79 9.11 12.00 10.53 8.91 9.67 10.12 6.86 7.11
AELDQLLHY 10.57 9.97 8.32 10.90 11.54 11.13 6.18 10.89 11.29 12.06 11.71 9.75 11.40 7.30 9.64 12.33 9.38 8.37 12.13 10.14 11.30 11.07 7.87 11.73 10.48 8.49 9.54 7.93 7.73 10.32 9.47 9.89 11.02 10.63 9.26 9.64 9.49
EDREEASLQDRQYASHHT 5.40 9.64 10.27 9.33 7.52 9.10 8.94 10.25 10.18 11.01 10.07 10.06 8.58 9.94 7.95 9.20 10.65 9.38 10.60 7.43 11.42 8.01 10.00 11.48 9.45 9.47 8.51 10.46 10.46 8.59 9.58 7.08 9.46 9.47 9.31 8.80 9.02
EDREEASLQDRQYASHHTTE 6.69 9.04 9.82 9.05 7.74 9.07 8.96 9.93 9.64 10.92 9.05 9.58 8.69 10.06 9.13 9.33 10.65 9.39 10.56 7.88 11.53 7.98 10.27 12.10 9.41 9.38 9.37 10.18 10.46 8.96 10.45 8.26 9.68 7.70 1.56 9.06 10.02
EEASLQDRQYASHHT 5.81 9.17 10.37 9.71 5.79 8.66 8.63 10.62 10.60 10.83 10.31 10.06 8.70 9.88 7.52 7.80 10.29 9.02 10.52 7.98 11.16 7.96 10.32 11.43 10.04 7.71 8.67 10.59 10.74 6.67 9.95 6.16 8.79 6.79 2.11 8.98 9.50
EEASLQDRQYASHHTTE 7.13 8.95 10.07 9.16 7.77 9.04 9.10 10.36 9.88 10.76 9.35 9.62 8.91 9.95 8.77 9.28 10.81 9.30 10.43 8.06 11.22 8.28 10.21 11.87 10.05 9.18 9.43 10.39 11.00 8.53 10.64 8.42 9.15 7.09 4.66 8.97 10.25
EESEESEEGTTSEVT 7.99 8.63 10.51 9.03 8.84 7.81 8.80 11.25 9.65 8.53 10.46 8.63 10.59 12.98 6.89 8.66 11.55 8.69 11.07 10.81 11.23 8.34 10.35 11.15 11.09 7.21 8.15 11.52 11.19 9.03 11.01 8.73 8.51 6.04 11.03 9.57 9.95
ELDQLLHY 10.01 10.56 10.06 9.39 9.94 9.30 9.41 9.99 10.34 10.81 10.45 8.85 11.07 9.65 10.41 9.74 10.52 8.32 10.39 9.62 10.82 9.11 9.63 11.81 11.41 8.24 11.27 10.47 10.93 6.61 9.95 8.71 9.21 5.04 9.84 7.61 10.22
FLGEAYHHVPE 8.95 8.33 9.36 8.53 9.52 9.30 8.32 9.40 6.85 11.18 8.55 8.78 9.89 8.80 10.29 10.55 8.23 7.03 10.90 10.01 9.39 10.97 7.99 10.79 10.59 7.00 8.88 8.19 8.96 9.26 8.82 7.35 10.24 10.31 4.55 10.06 8.35
FLGEAYHHVPES 8.91 9.35 10.03 8.37 9.72 9.01 9.42 10.37 7.82 11.13 9.49 9.44 10.33 8.98 9.04 9.05 9.72 10.10 10.56 10.16 9.90 10.17 8.02 10.47 10.68 6.18 9.30 9.33 11.01 8.78 9.62 7.69 9.90 7.01 7.74 9.01 8.16
FLGEAYHHVPESQ 8.74 9.57 9.83 8.67 9.72 6.30 9.61 10.45 7.96 11.18 8.86 9.66 9.66 9.55 8.86 9.12 9.80 8.23 10.34 9.84 9.72 9.21 7.53 11.45 10.74 7.27 10.05 10.22 11.01 6.40 9.65 8.95 9.96 8.52 8.06 9.14 8.98
FLGEAYHHVPESQRD 7.95 8.93 9.56 8.91 9.40 8.54 8.80 9.79 7.80 11.19 8.35 8.43 9.45 9.61 10.00 9.04 10.82 8.81 10.47 9.73 10.68 9.23 8.50 12.49 10.12 7.72 11.25 9.91 10.47 9.14 10.63 9.40 10.28 9.43 4.33 8.79 9.05
FLGEAYHHVPESQRDKA 6.00 8.73 9.47 9.43 8.43 8.68 8.72 9.70 7.86 11.23 7.50 9.10 8.88 9.68 9.91 9.54 10.82 8.03 10.40 9.41 10.15 10.59 9.39 12.41 9.33 7.66 10.61 9.25 10.39 9.19 11.12 9.26 11.17 8.25 9.89 9.56 9.57
GEAYHHVPESQRD 6.76 9.37 10.73 8.46 7.57 6.74 8.88 10.24 8.80 10.99 9.83 9.61 10.53 10.08 10.63 10.72 9.83 7.33 10.63 8.74 10.73 10.15 10.53 11.71 9.95 8.30 9.80 10.26 10.65 8.42 11.49 9.05 9.03 8.37 9.54 9.77 9.65
GEAYHHVPESQRDKA 3.58 8.63 9.67 8.97 7.91 8.64 7.98 9.52 8.59 10.91 7.45 9.98 9.95 9.42 10.87 10.28 10.27 6.85 10.11 8.74 9.96 11.21 11.01 12.15 9.49 6.91 9.65 8.28 10.28 8.76 11.67 8.35 10.53 7.98 9.85 8.84 9.15
GVLFNPYYDPLQWKSSRFE 12.69 9.77 9.42 8.88 9.76 10.07 11.92 8.37 9.91 12.94 9.09 11.36 9.97 7.77 10.52 12.66 10.16 7.67 10.04 8.62 10.71 11.11 9.91 12.09 9.94 10.71 11.27 9.78 12.23 10.36 10.74 13.55 8.88 11.09 10.23 10.17 10.93
LDQLLHY 8.11 9.63 9.81 8.82 9.97 8.13 8.17 10.96 9.87 11.65 9.09 7.78 11.28 9.55 10.30 9.28 10.04 9.06 10.78 10.15 11.08 10.50 9.76 11.59 10.47 7.35 10.54 9.50 9.73 8.03 10.13 8.60 10.51 10.06 10.35 8.83 9.55
LGEAYHHVPESQRD 7.39 9.11 9.57 8.70 9.38 5.44 7.59 9.89 6.99 11.56 7.39 7.57 9.72 9.78 9.90 9.49 10.92 8.01 10.76 9.78 10.86 9.69 7.52 12.50 10.32 6.43 11.19 9.51 10.17 8.44 10.84 9.68 11.09 9.02 9.53 8.93 5.32
LGVLFNPYYDPLQWKSSRFE 13.08 9.29 9.36 11.03 12.12 11.17 13.40 10.08 10.92 13.16 10.70 11.32 8.71 8.29 8.79 13.64 9.51 9.66 10.39 9.71 11.61 11.14 7.47 13.50 11.01 12.16 12.03 11.03 12.36 12.94 12.45 14.02 10.87 10.84 10.76 8.99 10.70
Q(-17.03)KIAEKFSQ 8.62 9.95 10.61 7.45 6.26 9.30 3.43 10.98 8.38 9.63 7.91 7.88 8.63 11.19 9.76 7.92 9.63 9.18 10.19 10.02 9.91 8.69 10.01 10.95 10.43 8.43 10.49 8.22 11.09 8.52 11.41 6.21 9.59 6.97 9.80 8.29 6.63
Q(-17.03)KIAEKFSQR(-.98) 3.76 8.69 9.74 5.99 5.28 9.05 2.44 10.25 8.52 9.46 6.28 8.30 8.71 10.31 10.47 8.80 10.86 7.85 10.48 10.41 11.22 9.56 10.70 11.74 10.07 9.46 10.24 7.66 9.81 9.51 11.89 6.92 10.81 8.67 8.28 9.29 6.69
Q(-17.03)YDRVAELDQLLHY 14.00 7.78 8.34 11.67 11.81 11.64 13.72 9.74 11.37 12.97 11.03 10.50 8.60 8.08 9.81 13.92 9.73 8.28 9.71 9.83 11.21 11.01 5.53 11.98 12.27 11.86 11.59 9.40 11.67 12.34 12.00 15.05 11.60 12.15 6.58 9.37 9.15
QYDRVAELDQLLHY 10.80 6.79 10.95 11.59 12.13 10.57 11.50 10.76 11.10 11.90 11.59 9.10 9.46 8.98 10.23 11.09 8.57 8.54 10.45 9.24 11.99 10.55 8.58 2.30 11.01 10.22 9.18 10.10 10.13 10.46 10.51 12.72 11.03 10.55 5.91 8.73 10.20
RVLTEEEEKELENL 10.55 10.54 10.86 8.73 10.30 12.08 10.78 11.73 10.61 10.73 9.79 9.11 9.65 11.64 11.26 10.23 8.50 8.09 12.37 12.15 10.78 10.03 10.52 10.19 10.77 10.13 10.55 10.24 11.76 11.18 13.38 8.67 10.02 9.42 6.98 9.37 7.82
SQGGEPGAYLTPDT 9.87 9.98 9.82 8.47 9.92 8.24 8.88 9.77 9.16 10.20 8.32 9.74 9.78 9.33 9.91 8.62 10.37 9.44 10.46 8.88 9.87 9.44 8.97 11.78 10.91 7.16 8.61 9.60 10.07 8.68 9.97 9.60 9.29 8.23 9.15 9.02 10.00
SQGGEPGAYLTPDTREE 8.84 8.77 9.30 8.37 10.16 8.85 8.97 8.95 9.18 10.37 8.61 9.14 9.81 9.75 9.28 9.27 10.26 9.40 10.34 10.01 9.59 8.93 8.98 10.82 9.64 7.51 10.34 8.78 9.51 9.02 9.83 9.21 9.43 8.32 9.81 8.59 9.60
SSQEGNPPSEERGHTLQEPEEAKVGE 8.08 9.51 9.27 8.77 10.08 9.93 10.30 9.97 9.15 10.89 8.94 10.10 9.32 10.25 9.43 10.84 10.84 9.58 11.02 9.19 10.99 9.74 9.19 11.86 11.36 9.62 10.87 10.74 9.97 10.69 9.88 10.30 11.00 7.58 7.55 10.72 9.86
VAELDQLLHY 9.47 10.70 9.96 10.98 11.73 10.72 10.09 10.61 10.87 7.10 10.78 8.98 10.38 9.10 10.57 10.96 9.08 8.96 11.06 9.95 11.40 10.39 8.52 12.45 10.73 9.33 9.99 10.26 10.19 10.18 10.54 11.37 10.31 10.15 10.19 7.82 9.08
YDRVAELDQLLHY 10.91 9.61 10.21 12.18 12.35 11.10 10.65 10.19 11.75 11.86 12.03 9.26 9.16 7.41 11.03 13.06 7.60 6.96 10.97 10.45 12.01 11.56 6.87 10.03 11.70 10.36 8.67 6.97 9.07 11.61 10.73 12.92 11.67 12.06 10.38 9.76 9.53
Q(-17.03)EPGAPAAGM 10.21 9.17 9.60 9.26 10.22 11.08 10.46 9.66 9.07 10.48 9.11 9.58 9.42 9.43 9.76 9.99 10.58 10.27 10.19 9.77 10.45 9.58 9.25 10.69 11.43 8.76 7.22 11.00 10.98 11.02 11.05 10.82 9.97 9.47 10.94 9.34 10.02
SLDSPAGPA 10.46 9.22 9.81 9.23 9.78 9.97 10.25 6.53 10.22 9.54 9.14 9.67 9.34 7.39 5.97 10.12 12.28 11.44 5.91 7.76 10.38 9.51 10.34 11.85 12.58 9.43 12.58 12.31 10.51 8.82 11.33 8.79 10.94 5.82 9.90 11.27 11.68
SLDSPAGPAE 7.81 9.60 10.15 8.34 9.62 7.84 10.05 6.79 10.59 8.53 8.76 9.85 8.61 8.42 5.29 2.66 12.52 11.46 2.70 7.01 9.61 9.50 10.16 11.95 12.17 7.40 12.57 11.41 10.32 7.61 10.69 7.36 11.00 7.88 2.53 10.89 11.48
VKVGVNGF 11.11 9.78 8.29 10.44 11.97 11.08 8.47 8.27 11.55 9.52 9.50 10.52 9.08 8.87 11.31 9.51 10.54 6.92 9.81 9.80 10.47 10.96 9.62 11.05 13.05 10.28 10.34 7.73 8.30 9.56 8.20 10.27 11.39 12.18 10.54 10.49 9.89
Q(-17.03)HWSYGLRPG(-.98) 9.68 9.79 9.09 9.16 9.81 7.29 10.37 8.39 8.09 10.27 9.55 5.79 9.61 8.45 7.05 6.48 11.09 11.09 6.02 8.35 10.03 8.09 2.83 10.39 10.39 9.13 11.04 10.53 11.09 9.25 10.08 7.45 10.60 9.96 5.07 9.09 9.05
SVSTVLTSKYR 9.00 10.06 9.30 9.30 11.68 11.45 7.60 8.97 10.28 9.72 9.54 10.05 7.94 9.78 9.18 8.36 10.85 6.36 10.02 11.36 9.67 10.81 9.48 10.55 11.42 8.59 9.74 6.21 7.21 10.19 9.22 9.70 10.94 10.62 10.82 10.59 10.26
GVANALSHKYH 6.41 10.47 9.48 10.23 10.81 8.89 7.85 9.75 11.08 10.61 10.23 9.68 7.77 10.29 9.71 10.06 12.04 6.38 10.75 10.81 10.80 11.17 6.17 11.14 11.13 10.59 5.94 8.90 6.86 10.34 7.31 9.83 11.46 11.10 5.93 4.28 10.19
KVVAGVANALSHKYH 10.34 10.26 9.02 9.74 11.21 10.01 7.79 8.90 10.20 10.91 9.64 9.85 10.47 10.21 10.15 10.07 11.98 10.53 11.00 10.49 11.00 11.40 10.27 11.38 11.04 10.21 10.37 8.56 10.73 11.12 10.74 10.21 11.75 11.47 10.55 10.36 9.92
VANALSHKYH 6.08 10.56 9.69 11.12 9.82 8.45 8.45 9.79 12.10 10.11 11.06 9.43 8.16 10.30 9.72 10.79 11.18 5.63 9.81 9.66 10.70 11.65 5.84 10.92 10.86 10.97 10.64 8.31 5.75 10.62 7.59 10.15 10.16 10.75 6.02 9.87 10.57
GRTLYGFGG 7.72 10.03 8.19 10.28 11.08 11.00 7.94 9.31 10.79 7.40 10.12 6.87 9.52 9.61 10.77 8.63 11.23 8.45 10.30 10.47 10.55 10.77 6.99 10.62 12.60 8.83 8.03 9.25 9.20 9.72 8.64 9.90 11.65 10.71 10.56 6.59 5.81
RTLYGFGG 7.80 9.93 7.78 10.58 11.30 10.73 7.45 9.12 11.08 8.23 10.10 6.90 9.33 9.63 10.58 8.37 11.33 8.33 10.09 10.38 10.37 10.82 7.58 10.22 12.81 9.10 8.15 9.26 9.29 9.17 9.14 9.54 11.64 11.30 10.65 7.22 6.82
TLYGFGG 10.07 11.36 10.37 11.13 12.06 11.40 11.90 7.84 11.40 8.76 11.17 5.19 12.44 7.78 10.92 9.69 9.82 11.91 8.81 8.67 8.98 10.58 10.18 9.77 12.36 8.99 12.00 10.95 11.12 8.32 9.95 8.97 10.64 11.96 8.55 9.14 10.36
AADQDLGPEAPPEGVLGA 11.50 9.39 7.47 8.49 10.36 10.30 12.25 10.04 9.58 11.67 9.56 10.50 8.95 6.86 10.59 10.69 9.59 7.45 8.84 8.94 9.67 10.37 9.02 9.84 10.32 10.06 10.97 10.60 9.72 11.06 8.81 11.64 10.15 7.85 10.08 10.85 11.12
AADQDLGPEAPPEGVLGAL 14.30 9.90 7.68 12.64 13.06 11.91 14.44 11.53 11.57 13.92 11.22 11.23 7.36 9.12 10.97 13.23 9.69 9.94 9.96 8.18 11.94 10.77 10.00 12.35 12.98 12.59 11.57 10.20 11.11 13.13 9.88 15.14 11.73 13.31 9.79 11.25 11.39
LENPQPQAPARRLLPP 10.22 9.72 8.78 8.94 9.86 9.65 10.44 7.56 7.11 9.93 9.53 9.93 9.84 8.72 9.69 9.61 9.71 10.62 8.04 8.89 9.61 9.68 7.69 10.32 11.64 9.69 10.06 10.17 10.48 11.18 10.08 9.61 10.96 8.95 5.10 10.35 9.29
VKMALQQEGFD 10.49 9.69 8.67 7.90 11.20 12.48 11.37 9.30 10.15 9.61 8.01 10.75 9.28 8.39 10.34 10.04 10.20 10.34 8.98 9.49 9.51 10.00 8.60 8.63 11.84 9.97 9.72 10.92 10.10 12.13 9.18 10.84 10.37 8.53 8.59 9.78 7.77
GGFMRGL 10.55 10.72 9.21 10.55 8.95 7.47 9.08 10.02 9.87 9.58 10.08 10.63 12.31 6.50 7.06 7.98 9.71 11.26 9.36 8.87 9.63 11.76 6.15 10.60 12.05 9.20 10.11 10.97 6.84 9.34 9.39 9.79 12.15 11.00 10.93 10.31 9.71
Q(-17.03)LEDEAKELQ 9.60 10.53 9.47 8.84 10.69 9.68 7.34 7.21 8.26 10.35 9.83 10.30 10.94 7.73 10.73 7.72 11.01 12.00 7.47 8.69 10.62 10.37 10.22 10.32 12.36 9.58 11.18 11.54 10.53 10.06 10.42 9.51 10.80 9.18 6.52 10.74 12.24
SPQLEDE 8.11 10.45 9.76 10.66 9.08 7.30 10.43 7.44 9.07 9.23 11.06 1.91 10.97 7.52 10.67 7.24 11.27 11.56 7.40 8.43 9.93 10.64 10.19 11.01 12.86 10.52 11.54 12.31 11.45 8.66 10.90 8.67 10.64 7.22 5.66 10.47 11.33
SPQLEDEAKEL 8.12 10.02 9.35 9.09 10.23 9.06 10.17 6.55 7.83 10.29 9.73 3.55 10.75 7.96 7.86 7.17 11.19 11.93 7.85 8.73 10.01 9.99 3.31 11.11 12.46 9.29 10.89 10.81 10.53 9.82 10.82 8.86 11.34 9.08 7.10 10.25 11.17
SPQLEDEAKELQ 7.92 9.86 9.21 8.69 10.06 9.06 9.65 6.20 7.74 10.14 9.38 9.75 10.50 7.71 7.54 7.14 10.64 11.35 8.15 8.26 9.26 9.73 9.72 10.23 11.60 8.39 10.89 10.42 10.16 8.82 9.82 7.87 10.28 8.30 7.31 10.05 10.89
VGRPEWWMDYQ 10.41 10.11 9.80 10.27 11.34 9.32 10.62 7.95 8.66 10.75 11.37 5.48 11.64 7.04 7.01 10.48 10.43 10.89 7.19 9.05 10.20 10.66 10.04 10.66 12.45 10.11 10.91 10.76 11.24 10.22 11.08 10.19 10.93 9.31 9.76 9.89 10.89
YGGFM(+15.99)RF 8.27 10.86 8.75 10.11 11.59 10.07 10.22 7.17 8.51 8.96 10.51 10.31 10.34 7.87 7.35 5.05 11.52 11.49 8.42 10.18 9.98 10.54 10.54 10.15 13.32 9.71 11.89 11.97 12.14 9.06 11.80 9.59 11.22 8.54 8.74 10.37 11.14
YGGFM(+15.99)RGL 8.57 11.03 9.51 9.72 11.44 8.55 9.87 6.38 8.65 10.02 10.51 10.53 10.51 7.72 6.91 10.68 11.58 11.71 8.55 9.85 9.67 10.69 10.60 11.19 13.25 9.69 11.86 11.98 11.71 9.31 11.77 9.71 11.57 8.36 9.24 11.07 11.32
YGGFMRF 8.96 10.07 9.36 8.61 10.57 7.93 10.60 6.82 7.30 10.14 9.89 4.65 11.14 6.84 7.13 6.06 10.87 11.00 6.51 8.41 8.86 9.16 3.35 11.13 12.44 9.29 11.62 10.68 10.93 8.55 9.98 8.57 10.20 7.64 4.33 9.52 10.32
YGGFMRGL 8.83 10.07 9.52 8.98 9.79 8.44 10.26 6.02 6.71 9.93 9.54 3.76 10.76 7.46 7.38 5.69 10.64 11.05 7.16 8.43 10.09 10.25 3.22 10.62 12.46 9.48 11.49 10.90 10.72 9.69 10.30 8.22 10.74 9.01 3.51 9.50 9.29
EIGDEENSAKFPI(-.98) 9.42 10.46 9.34 8.24 11.66 10.22 10.67 9.46 9.70 9.74 9.37 7.32 10.45 9.69 6.56 7.73 10.72 11.13 9.58 8.71 10.58 9.76 6.18 8.26 11.00 9.67 11.16 10.86 10.26 8.98 9.54 9.77 11.51 8.44 10.74 10.74 10.34
DDGPYRMEHFRWGSPPKD 9.79 9.91 9.60 7.32 10.09 11.57 11.03 9.42 9.45 12.87 8.69 10.77 9.04 8.48 11.23 10.49 9.58 8.51 9.65 9.54 10.38 11.23 8.97 9.38 8.61 10.38 9.03 10.09 8.93 13.08 7.83 10.80 10.64 8.51 10.04 10.35 7.30
FKNAIIKNAH 8.07 11.14 9.30 7.80 8.84 9.07 11.47 10.57 11.03 10.87 9.53 10.27 8.75 9.35 11.21 11.39 11.10 4.31 9.09 9.42 9.70 10.25 10.94 10.07 10.33 10.99 10.20 11.29 8.82 12.22 9.85 10.35 10.88 8.04 9.94 10.83 9.98
FKNAIIKNAHKKGQ 10.06 9.27 9.14 8.49 8.43 6.28 10.37 10.86 10.84 9.93 12.15 8.03 9.44 10.48 9.88 12.16 7.21 9.39 9.95 9.73 11.37 9.31 11.24 10.82 7.84 10.79 4.73 8.84 8.74 10.32 8.17 9.82 6.49 8.04 8.86 8.89 5.56
GGPEFRDDGAEPGPRE(-.98) 7.53 8.68 9.01 9.71 11.31 10.69 7.80 9.02 10.43 10.60 9.17 7.78 7.35 8.80 10.96 10.85 10.06 7.34 10.22 11.90 11.78 10.14 6.17 10.73 11.73 10.47 8.46 8.00 9.06 10.18 8.45 9.24 10.75 7.52 8.01 6.67 6.87
GPEFRDDGAEPGPRE(-.98) 7.85 8.94 9.20 10.77 11.43 10.50 7.90 8.93 10.56 10.08 10.19 7.34 7.30 8.66 10.89 11.01 10.14 7.37 10.27 10.83 12.07 10.36 6.52 10.92 11.77 10.87 8.72 8.26 9.25 9.88 8.54 9.23 10.65 10.77 8.89 7.35 7.17
KYVMGHFRWD 9.38 9.84 8.93 6.31 11.15 11.79 10.30 9.80 9.68 10.86 7.04 10.22 7.76 8.31 10.86 9.73 9.64 8.51 8.90 10.03 9.88 10.73 9.69 8.01 9.34 9.83 8.39 10.00 7.42 11.67 8.95 10.26 10.07 8.17 9.64 10.18 9.09
KYVMGHFRWDRF(-.98) 9.03 9.25 7.70 6.69 10.60 11.63 10.16 9.43 9.62 10.54 7.78 10.20 7.80 7.85 11.25 10.27 9.73 8.44 9.15 8.81 10.47 10.82 9.24 9.32 7.61 10.10 9.02 8.79 8.19 12.44 7.08 10.09 11.41 8.48 9.98 10.36 6.33
PEFRDDGAEPGPRE(-.98) 6.67 9.22 8.82 10.96 11.10 10.35 8.31 9.56 10.94 10.46 9.47 7.68 7.33 8.91 10.85 10.73 10.59 7.81 10.65 10.95 12.09 10.13 9.80 10.68 11.24 10.55 9.38 7.53 9.77 9.65 8.38 8.41 10.21 10.02 7.56 7.92 7.44
RPVKVYPNGAEDES(+79.97)AESFPLEF 13.43 10.00 9.23 9.65 10.88 12.42 13.65 10.32 10.98 1.18 9.94 9.94 7.47 7.22 10.33 13.47 7.10 8.45 8.82 10.36 9.04 10.36 6.49 8.64 12.05 10.93 9.36 10.36 9.97 12.04 9.13 13.09 9.17 8.69 6.63 8.30 9.74
RPVKVYPNGAEDESAESFPLEF 13.04 8.69 8.40 9.37 11.63 13.45 11.73 11.30 10.87 11.42 9.96 11.47 9.00 6.74 11.60 11.37 7.50 6.77 9.43 10.91 8.79 8.28 6.91 10.45 10.96 9.19 8.73 9.75 8.32 10.79 8.72 12.94 7.24 8.31 7.03 8.77 9.75
S(+42.01)YSMEHFRWGKPV(-.98) 10.50 10.01 9.22 7.76 10.85 12.32 11.21 9.57 9.55 11.96 7.80 10.49 7.79 8.09 11.08 10.36 9.13 7.66 9.28 10.77 9.76 10.92 8.47 10.13 10.63 9.68 9.59 10.15 9.12 12.62 7.73 11.56 11.09 8.25 4.33 10.11 5.40
SQTPLVTLF 10.98 10.39 10.66 11.26 11.25 11.21 10.77 11.17 9.90 10.55 10.94 8.99 9.92 9.96 10.57 9.85 9.51 9.61 10.82 10.27 10.73 7.92 10.09 9.40 11.78 8.53 11.70 10.90 10.86 10.40 10.22 11.48 8.78 8.44 11.95 4.18 9.98
SQTPLVTLFKNAIIKNAHKKGQ 6.88 9.56 8.13 10.84 10.09 10.91 10.04 9.10 10.32 14.70 11.30 7.34 6.58 5.13 7.78 9.99 10.14 9.62 10.47 9.80 11.38 9.10 9.77 12.25 9.63 9.01 10.22 9.93 10.59 8.75 7.88 10.66 9.50 9.10 9.04 9.69 10.82
SYSMEHF 7.98 8.83 10.34 9.49 10.21 10.24 8.90 10.46 9.37 10.16 9.77 9.22 9.09 10.02 10.40 8.60 10.50 10.37 10.12 10.31 10.70 9.22 10.50 10.69 10.87 7.95 9.56 10.64 10.21 9.59 10.73 10.56 9.42 10.42 11.24 8.59 10.50
SYSMEHFRWGKPV(-.98) 9.77 8.97 9.33 7.34 10.25 10.23 9.63 9.41 8.14 11.99 8.51 10.15 8.79 8.54 10.39 10.51 10.10 9.75 10.39 9.42 11.20 10.03 8.62 9.97 8.91 8.91 9.18 8.70 9.15 9.81 8.56 9.24 9.28 10.47 2.00 9.72 9.52
TPLVTLFKNAIIKNAHKKGQ 5.29 5.89 8.34 12.55 11.97 10.75 8.05 5.89 11.30 9.99 9.26 10.37 9.19 5.69 11.33 11.68 7.19 2.47 9.23 9.24 11.43 9.83 9.47 11.20 10.32 11.73 7.02 6.85 6.95 9.21 6.97 10.32 10.41 12.97 10.63 10.74 9.89
Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLFKNAIIKNAH 13.79 11.21 7.31 7.23 10.67 13.14 13.79 8.84 11.43 11.27 8.55 10.24 10.02 9.55 11.01 11.38 9.38 9.00 9.02 9.74 8.77 11.78 7.31 9.81 11.83 11.50 11.19 11.52 9.54 12.51 8.35 13.69 10.65 7.62 3.17 10.97 9.32
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNA 14.53 8.58 9.13 1.49 11.68 14.27 14.53 8.60 10.27 12.43 8.79 11.08 5.78 8.92 8.17 12.64 7.09 7.35 7.06 8.75 7.22 10.59 9.13 8.77 11.10 11.16 9.20 10.07 8.34 13.37 7.10 14.38 10.10 7.09 9.76 10.60 8.97
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAH 13.37 10.36 8.35 7.54 10.62 12.61 13.54 9.77 10.62 12.13 8.53 10.89 6.87 7.00 10.96 11.58 9.20 8.33 8.98 9.29 9.86 11.55 8.32 10.33 11.80 11.59 11.17 11.56 9.46 13.63 7.62 13.08 10.33 7.26 0.34 10.22 6.53
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAHKKGQ 10.70 11.20 8.85 8.34 6.96 11.49 10.78 10.94 9.53 11.60 9.84 9.07 8.09 8.69 11.03 9.96 8.84 9.35 10.24 7.84 10.25 11.13 9.66 9.14 9.29 9.56 10.50 9.84 8.18 12.42 8.34 11.71 9.49 7.10 2.50 9.99 8.01
YGGFM(+15.99)TSEKSQTPLVTLFKNAIIKNAHKKGQ 9.74 10.61 9.41 11.69 9.95 8.71 10.58 10.91 10.24 11.07 11.85 7.56 6.80 10.37 8.55 10.69 11.51 11.45 11.31 9.79 10.90 8.22 10.17 12.35 9.12 10.23 12.76 11.00 11.92 8.44 10.31 10.50 3.55 9.21 6.44 8.86 12.67
YGGFMTSE 9.45 8.85 9.86 9.18 10.35 10.79 9.51 10.37 8.88 12.02 9.13 8.98 9.11 10.30 10.12 9.56 10.14 10.67 10.28 9.71 10.57 9.59 10.29 10.45 11.03 8.00 11.45 10.96 10.23 10.55 10.31 11.69 9.09 9.87 11.38 8.38 10.06
YGGFMTSEKSQTPLVTLFKNAIIKNA 11.53 10.59 9.34 9.43 11.70 12.68 11.78 10.45 10.75 12.55 9.69 11.41 7.53 7.97 10.72 12.93 9.67 10.65 10.66 9.63 11.43 11.93 8.74 10.78 13.53 11.50 11.32 11.16 11.60 12.37 11.09 12.75 10.41 10.53 10.99 11.22 10.04
YGGFMTSEKSQTPLVTLFKNAIIKNAH 9.77 11.31 9.97 8.89 11.36 11.82 9.96 9.99 9.42 10.83 7.78 9.82 9.82 8.25 11.18 12.08 6.43 9.25 9.47 8.80 10.22 11.49 9.17 7.66 12.17 10.08 9.05 11.05 10.03 11.28 9.51 11.88 9.05 8.66 10.05 11.07 8.98
YGGFMTSEKSQTPLVTLFKNAIIKNAHKKGQ 8.62 9.70 9.82 10.83 9.92 9.01 10.44 11.40 11.18 11.55 11.83 9.24 9.75 9.75 9.51 9.68 10.38 9.96 10.18 8.77 11.40 8.53 10.03 11.79 8.28 9.52 11.60 9.68 10.19 8.53 9.68 9.96 8.37 7.53 5.91 8.42 11.34
ATLESVFQELGKLTGPSSQKRE 11.69 9.82 9.87 10.89 9.60 11.48 10.80 9.91 10.36 11.69 11.44 10.98 11.15 8.70 11.50 10.22 10.23 11.30 12.37 10.20 10.91 11.05 10.75 12.06 10.48 7.58 11.16 6.60 11.18 7.76 12.93 9.08 8.75 9.47 9.50 9.51 10.71
MYEENSRDNPF 8.25 9.15 9.89 8.57 9.60 9.73 9.23 9.93 9.98 10.47 9.10 9.14 9.94 9.55 9.71 8.29 10.35 9.47 10.67 9.25 10.40 9.51 10.00 10.45 11.49 7.62 9.34 10.13 11.28 8.76 11.62 9.77 9.04 9.36 8.38 8.27 8.71
TNEIVEEQYTPQSL 11.18 10.38 11.75 8.73 10.29 11.78 8.18 10.49 11.49 11.77 8.13 8.71 10.43 10.21 11.31 10.97 9.46 5.39 12.54 11.79 10.52 10.72 11.09 11.62 11.56 9.36 11.78 8.10 12.17 9.55 13.71 10.08 9.92 9.40 11.53 9.60 7.09
FPTLGGSQDKSLHN 9.38 8.53 9.12 7.79 9.22 9.24 8.54 9.20 8.54 10.61 8.43 9.45 9.39 9.18 10.14 8.99 10.94 9.76 9.49 9.86 10.48 9.77 9.86 10.53 11.02 8.61 10.98 9.65 10.26 9.90 10.67 8.76 10.18 8.77 10.34 9.16 9.20
TLGGSQDKSLHN 3.23 9.21 9.65 9.13 6.86 7.32 8.45 10.04 11.08 10.19 9.48 8.49 9.79 9.73 10.88 10.02 10.97 8.20 9.10 8.53 11.65 9.80 10.99 11.19 10.39 9.22 10.16 9.23 10.52 8.34 10.70 7.84 9.47 8.79 8.76 5.68 8.61
SVNPYLQGQRL 8.92 8.68 9.52 5.27 6.44 9.67 4.50 10.18 9.00 8.96 5.73 8.53 9.25 9.66 11.22 7.62 10.60 6.97 10.03 9.70 10.83 9.05 9.97 10.40 10.20 8.12 10.05 7.18 9.99 8.78 11.79 6.31 9.79 10.27 10.16 8.17 4.95
SVNPYLQGQRLDNVV 11.90 9.25 9.73 11.00 11.28 12.70 11.87 9.55 10.75 11.43 10.66 10.58 4.12 8.67 9.60 11.56 8.75 8.99 10.30 10.19 11.34 9.42 7.92 11.23 12.27 11.14 11.44 10.64 11.16 12.10 9.95 12.53 9.51 10.86 10.59 9.25 9.29
SVNPYLQGQRLDNVVA 11.40 9.34 9.39 10.53 11.03 11.59 11.30 9.49 10.04 11.59 10.16 10.59 9.05 8.66 10.78 10.69 10.07 9.28 10.14 9.84 11.65 9.93 8.66 11.88 11.96 10.39 11.52 10.34 11.04 11.63 10.31 11.70 10.43 10.36 10.59 8.42 9.23
SANSNPAM(+15.99)APRE 10.30 11.23 10.37 10.15 9.61 4.40 9.84 4.95 9.15 9.43 10.82 10.55 9.91 5.50 2.44 5.16 12.00 11.50 8.08 7.34 10.82 10.23 10.65 12.02 11.91 9.89 10.62 11.22 12.67 6.97 11.61 4.98 11.06 8.22 10.40 11.31 10.86
SANSNPAMAPRE 7.76 10.06 10.00 9.30 9.31 5.83 10.39 6.02 8.34 10.83 9.52 5.02 9.72 5.61 7.55 6.40 11.68 10.93 6.64 8.75 10.79 9.28 3.94 11.64 11.83 10.02 11.03 11.56 11.25 8.27 10.33 6.44 10.68 8.89 6.99 9.70 10.05

After imputation using knn (k = 7), the data was normalized using EigenMS to account for sample-to-sample variability.

EigenMS normalization preserves the treatment group differences in the data by estimating treatment effects with an ANOVA model, then uses singular value decomposition on the model residual matrix to identify and remove the bias.

Code
plot_grid(hist_eigenms, qq_eigenms, pca_eigenms, nrow = 1)

Code
norm_d1_mod = norm_d1[-1,] %>%
        as.data.frame() %>%
        rownames_to_column(., var = "rowname") %>%
        mutate(across(-rowname, as.numeric)) %>%
        column_to_rownames(., var = "rowname")
#write.csv(norm_d1_mod, file = "after_norm_data.csv")

#rownames(norm_d1_mod) == feature_name$variable
rownames(norm_d1_mod) = feature_name$name

# display table
norm_d1_mod %>% 
        mutate(across(everything(), round, digit=2)) %>%
        knitr::kable(., digits = 3, "html") %>%
        kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
        kableExtra::scroll_box(width = "100%", height = "500px")
Pituitary_53 Pituitary_31 Pituitary_38 Pituitary_63 Pituitary_55 Pituitary_51 Pituitary_50 Pituitary_34 Pituitary_59 Pituitary_43 Pituitary_66 Pituitary_26 Pituitary_19 Pituitary_24 Pituitary_41 Pituitary_57 Pituitary_47 Pituitary_52 Pituitary_23 Pituitary_58 Pituitary_44 Pituitary_37 Pituitary_39 Pituitary_46 Pituitary_65 Pituitary_56 Pituitary_48 Pituitary_35 Pituitary_61 Pituitary_49 Pituitary_60 Pituitary_54 Pituitary_45 Pituitary_42 Pituitary_62 Pituitary_25 Pituitary_64
AGAPEPAEPMELAKPSA 12.03 9.92 10.59 6.66 13.59 12.75 11.30 4.23 9.24 8.51 9.25 6.88 5.87 9.45 11.35 12.35 10.60 12.74 10.60 5.48 11.92 11.52 8.28 9.18 7.20 10.74 8.94 9.67 7.42 12.04 8.01 11.65 7.99 9.38 8.49 10.34 8.72
LAGAPEPA 8.74 8.21 11.22 7.57 10.60 11.67 10.90 8.25 5.13 8.47 7.36 8.96 8.93 9.75 9.28 13.71 7.93 12.96 7.11 6.94 3.42 10.97 6.13 6.70 4.96 12.27 5.28 9.74 7.79 11.91 6.93 11.21 5.78 6.42 6.62 9.56 6.13
AFGPRGPGLQL 9.41 8.75 9.50 10.25 10.05 8.95 10.09 9.84 5.16 10.93 9.56 9.36 9.49 9.49 10.30 9.34 8.80 8.27 9.02 9.16 10.07 8.75 9.14 10.24 10.00 9.55 10.10 9.61 9.78 9.40 10.00 11.15 9.76 11.03 9.74 7.76 10.62
AAMDLEL 10.02 5.12 9.15 8.77 12.37 4.89 9.73 8.42 9.16 9.86 9.13 8.67 9.42 8.39 10.08 8.06 8.92 7.61 7.92 8.71 8.24 10.39 7.90 9.86 6.63 9.10 10.09 7.72 9.65 9.35 11.95 10.82 9.62 8.36 8.73 8.86 8.48
AELDQLLHY 11.35 10.00 8.39 10.05 10.59 10.57 6.93 10.05 10.39 12.29 11.51 10.24 11.44 7.58 8.88 11.45 10.26 10.77 10.73 9.16 10.86 9.77 9.26 11.88 9.31 8.24 10.81 8.56 8.71 9.71 10.19 9.24 10.41 9.91 10.30 10.25 10.85
EDREEASLQDRQYASHHT 5.79 9.90 10.00 9.34 7.84 9.26 8.85 9.71 10.18 10.73 9.88 10.69 8.86 9.31 8.10 8.81 10.34 8.57 9.89 7.52 10.76 8.81 9.30 10.70 9.83 9.52 8.39 10.51 9.64 8.77 8.78 7.27 10.42 10.50 10.68 9.63 9.00
EDREEASLQDRQYASHHTTE 7.59 8.86 9.49 8.62 8.32 9.47 7.86 8.84 9.01 9.64 7.86 10.58 9.47 9.56 9.92 8.74 10.30 8.79 9.52 8.35 10.05 8.83 10.35 10.32 8.80 9.58 8.96 9.39 8.63 9.23 9.22 8.22 11.57 10.32 7.69 10.29 9.36
EEASLQDRQYASHHT 6.62 8.95 10.04 9.16 6.53 9.23 7.27 9.60 9.96 9.47 8.93 11.00 9.50 9.39 8.46 7.33 9.84 8.13 9.68 8.66 9.68 8.98 10.31 9.62 9.60 7.89 8.08 9.63 8.81 7.09 8.73 6.18 10.84 9.47 8.62 10.11 8.48
EEASLQDRQYASHHTTE 7.78 8.84 9.79 9.34 8.40 9.23 8.30 9.55 9.61 9.61 8.71 10.37 9.62 9.41 9.43 8.81 10.39 8.56 9.67 8.47 10.06 9.15 9.98 10.27 9.56 9.51 8.91 9.74 9.17 8.69 9.25 8.47 10.78 9.65 9.13 10.02 9.81
EESEESEEGTTSEVT 8.68 8.78 10.01 10.04 8.81 7.66 9.38 10.79 10.46 8.51 11.16 10.10 10.38 12.05 7.42 8.46 10.80 7.12 10.16 10.65 10.45 9.16 9.59 9.78 10.67 7.47 7.85 11.57 9.65 8.94 9.16 9.24 9.19 8.05 11.59 10.87 10.84
ELDQLLHY 10.41 10.35 9.90 10.52 10.17 8.96 9.37 9.70 10.68 10.09 10.87 9.38 11.37 9.32 10.87 9.56 10.14 7.92 10.04 9.67 10.26 9.40 9.59 10.64 10.38 8.74 10.83 10.06 9.42 6.36 8.52 8.79 9.83 7.24 11.49 8.27 10.62
FLGEAYHHVPE 9.69 7.94 9.43 8.38 8.91 8.75 8.50 8.74 6.19 10.75 8.37 9.27 10.08 9.17 10.19 9.98 8.75 8.91 9.97 9.46 8.82 9.85 9.57 10.30 8.69 7.11 9.47 8.07 8.86 8.65 8.80 6.79 9.99 11.03 7.18 10.58 9.29
FLGEAYHHVPES 9.45 8.86 9.87 9.44 9.46 8.61 9.58 10.22 8.27 10.70 10.05 10.31 10.17 8.91 9.70 9.11 9.35 9.90 10.27 10.04 9.43 9.93 8.67 9.37 8.90 6.60 8.98 8.81 9.71 8.38 8.33 7.77 9.92 9.00 9.40 9.57 8.90
FLGEAYHHVPESQ 9.08 9.09 9.76 9.22 9.62 6.16 9.40 10.38 8.14 10.72 8.97 10.16 9.59 9.65 9.48 9.27 9.54 8.11 10.32 9.94 9.38 9.01 8.20 10.67 9.41 7.55 9.71 9.61 10.06 6.20 8.87 8.97 10.05 9.98 10.01 9.38 9.16
FLGEAYHHVPESQRD 8.80 8.49 9.29 9.13 9.20 8.48 8.56 9.19 7.76 10.60 8.13 9.63 9.45 9.44 10.78 8.86 10.51 8.60 9.71 9.74 9.73 9.19 9.27 11.11 8.53 7.93 11.06 9.29 9.11 8.96 9.48 9.42 10.85 11.50 8.11 9.71 9.42
FLGEAYHHVPESQRDKA 6.34 8.71 9.37 10.77 8.46 8.05 9.22 9.44 8.26 10.80 8.34 9.48 9.13 9.34 9.91 9.19 10.66 8.14 9.90 9.10 9.82 10.63 9.27 11.60 8.40 8.14 10.50 9.30 9.35 8.72 9.90 9.26 11.37 9.88 9.86 10.20 10.53
GEAYHHVPESQRD 7.08 9.58 10.64 9.32 8.00 6.33 8.99 9.68 8.78 10.27 10.13 9.75 11.24 9.58 10.45 10.06 9.78 7.45 9.93 8.61 10.18 10.61 9.99 10.87 9.74 8.71 9.71 10.37 9.60 8.18 10.43 8.95 9.89 9.97 10.42 10.54 10.09
GEAYHHVPESQRDKA 4.07 8.91 9.59 10.47 8.03 7.69 8.81 8.87 8.77 10.32 8.45 10.26 10.61 8.85 10.36 9.36 10.37 7.57 9.03 8.12 9.44 11.28 10.60 11.27 8.73 7.48 9.86 8.76 9.27 8.09 10.33 8.16 10.97 9.73 9.50 9.85 10.57
GVLFNPYYDPLQWKSSRFE 12.04 9.65 9.99 10.13 10.85 9.38 11.15 8.50 9.32 11.34 9.19 9.34 11.52 8.25 9.90 12.20 10.73 9.45 10.78 8.90 11.05 10.93 9.88 12.18 9.65 11.50 10.85 9.43 11.70 9.98 10.74 12.84 9.43 12.16 10.60 9.42 10.34
LDQLLHY 8.70 9.65 9.54 9.12 9.43 7.88 8.90 10.63 10.23 11.99 9.59 8.87 10.85 9.20 10.45 9.11 9.85 8.82 9.98 9.69 10.67 10.38 9.93 11.04 9.75 7.34 10.80 9.74 9.37 7.77 9.43 8.77 10.33 10.65 10.36 9.62 10.62
LGEAYHHVPESQRD 8.32 8.88 9.23 9.97 8.51 4.69 8.80 9.49 7.77 11.79 8.60 9.19 9.13 9.38 10.25 9.29 10.58 7.91 9.68 9.02 10.28 9.23 8.11 11.37 8.35 6.73 11.41 9.65 9.14 7.73 9.29 9.87 10.64 10.72 9.61 10.10 7.29
LGVLFNPYYDPLQWKSSRFE 13.00 8.91 9.74 11.88 12.54 10.53 12.89 9.96 10.38 11.90 10.71 10.34 9.68 8.80 8.57 13.26 9.98 11.40 10.60 9.73 11.57 10.54 8.25 13.21 9.76 12.75 11.87 10.56 11.78 12.45 12.28 13.39 11.10 12.10 12.45 8.70 10.66
Q(-17.03)KIAEKFSQ 9.45 10.29 10.13 9.61 6.08 8.40 4.90 10.38 9.52 9.59 9.64 9.36 8.59 10.01 9.78 7.29 9.02 8.25 8.81 9.29 9.17 9.28 9.04 9.43 9.53 9.02 10.45 8.79 9.33 7.88 8.94 6.62 9.99 9.49 8.52 9.97 8.73
Q(-17.03)KIAEKFSQR(-.98) 4.91 9.13 9.29 7.98 4.80 7.82 4.31 9.23 9.30 9.45 8.06 9.95 8.80 9.14 10.07 7.68 10.62 7.92 8.40 9.22 10.25 9.71 10.11 10.22 8.79 10.01 10.74 8.59 8.36 8.58 9.58 7.05 11.07 11.04 7.20 11.32 9.47
Q(-17.03)YDRVAELDQLLHY 14.27 7.09 8.88 11.42 11.77 10.99 12.91 9.23 9.90 11.57 10.22 9.55 9.65 9.18 9.47 13.26 10.80 11.62 9.53 9.64 10.93 9.45 7.72 11.88 10.12 12.22 12.00 8.77 11.75 11.68 12.73 13.90 11.55 12.87 10.65 9.01 9.12
QYDRVAELDQLLHY 11.24 6.66 11.19 9.79 11.84 10.65 10.94 9.98 9.52 11.51 10.16 8.88 9.99 9.58 9.74 10.39 9.52 10.83 9.67 9.01 11.51 9.60 10.03 2.58 10.48 9.87 10.04 10.10 11.11 10.37 11.88 11.92 11.14 9.68 9.37 8.79 9.94
RVLTEEEEKELENL 11.51 10.70 10.73 9.60 10.24 11.18 11.39 10.56 10.24 9.89 10.20 9.83 10.47 11.12 10.82 8.98 8.79 9.39 10.66 11.42 9.73 9.86 10.74 8.93 9.44 10.60 11.03 10.59 10.65 10.47 12.11 8.25 10.75 11.51 9.01 10.81 9.30
SQGGEPGAYLTPDT 10.08 9.72 9.75 9.40 9.92 7.92 8.97 9.75 9.52 9.82 8.80 10.06 9.81 9.25 10.25 8.65 10.13 9.29 10.41 8.85 9.67 9.40 9.17 11.11 9.94 7.52 8.31 9.29 9.15 8.41 9.07 9.65 9.38 9.59 9.79 9.29 10.42
SQGGEPGAYLTPDTREE 9.05 8.78 9.17 9.20 10.16 8.56 9.32 8.84 9.59 10.23 9.18 9.55 9.82 9.44 9.41 9.17 10.01 9.05 10.06 9.87 9.38 9.11 8.75 10.27 9.20 7.77 10.19 8.81 8.80 8.81 8.95 9.34 9.57 9.34 9.59 9.03 10.16
SSQEGNPPSEERGHTLQEPEEAKVGE 8.47 9.05 9.26 9.67 10.11 9.51 10.10 9.74 9.19 10.07 9.17 10.40 9.61 10.35 9.86 10.70 10.74 9.98 10.83 9.18 10.55 9.46 9.89 10.91 9.76 10.10 10.57 10.16 8.78 10.30 8.91 10.14 11.27 9.49 9.78 11.08 10.23
VAELDQLLHY 10.36 10.70 9.74 10.43 10.78 10.45 10.90 9.92 10.65 7.60 10.81 10.23 9.93 8.99 10.45 10.49 9.31 9.73 9.74 9.20 10.81 9.70 9.41 12.12 9.70 9.06 10.82 10.68 10.49 9.81 10.49 11.24 9.89 10.06 11.15 8.73 10.38
YDRVAELDQLLHY 11.52 9.63 10.61 11.04 11.43 10.25 11.27 9.25 10.15 11.78 11.56 8.91 9.69 8.15 9.67 11.82 9.14 10.99 9.58 9.29 11.77 9.62 8.80 10.73 10.38 10.13 10.33 7.75 10.74 10.76 12.28 11.74 10.85 10.67 11.50 9.97 10.87
Q(-17.03)EPGAPAAGM 10.35 9.01 9.61 10.39 10.13 10.54 10.85 9.68 9.45 10.21 9.86 9.71 9.48 9.39 9.81 9.92 10.49 10.52 10.10 9.53 10.41 9.35 9.42 10.26 10.47 9.15 7.10 10.94 10.33 10.59 10.28 10.79 9.80 10.53 10.56 9.55 10.75
SLDSPAGPA 9.68 7.84 9.89 11.29 10.25 10.26 8.98 8.21 11.77 8.80 9.83 9.36 8.65 8.09 8.08 12.12 11.00 9.11 8.60 9.09 11.12 9.69 10.91 11.11 10.85 10.19 10.47 10.19 8.47 9.00 9.94 9.46 10.57 8.20 10.78 9.89 10.32
SLDSPAGPAE 7.16 7.35 10.10 8.96 9.84 9.22 7.43 8.82 11.98 7.81 8.07 10.25 7.13 9.72 8.90 5.73 10.75 7.95 6.21 9.21 10.14 9.54 12.02 10.92 9.71 7.75 9.85 7.94 8.05 8.43 9.75 8.31 10.64 10.33 7.55 9.02 8.61
VKVGVNGF 11.05 9.49 8.70 10.16 11.21 10.46 8.92 8.39 10.97 9.74 9.65 9.87 8.99 9.77 10.63 9.36 11.44 9.41 9.86 9.16 10.99 9.31 11.18 12.00 11.89 10.20 11.14 8.00 9.66 8.95 9.45 9.60 10.05 10.81 9.64 9.86 10.66
Q(-17.03)HWSYGLRPG(-.98) 10.03 8.08 8.76 8.68 8.77 8.32 9.28 9.43 9.06 10.71 8.99 7.58 7.58 9.36 9.80 8.63 9.88 8.76 7.50 9.29 10.00 7.40 5.17 9.55 7.74 8.88 9.87 8.37 10.07 9.66 9.61 8.21 9.59 11.04 9.29 8.45 8.10
SVSTVLTSKYR 9.10 9.96 9.59 9.63 11.00 10.59 8.43 8.91 9.97 9.88 10.16 9.67 7.97 10.30 8.39 7.97 11.62 8.60 9.69 10.54 10.00 9.44 10.57 11.12 10.26 8.68 10.54 6.71 8.12 9.43 9.83 9.14 9.83 9.89 9.36 10.40 11.54
GVANALSHKYH 7.82 10.35 9.42 8.18 9.37 8.57 8.45 8.32 9.57 10.94 9.21 11.01 7.62 10.66 9.14 8.93 13.10 9.24 8.54 9.66 9.75 9.49 8.46 11.01 9.39 9.98 7.67 9.44 8.07 9.77 8.37 9.02 10.94 10.24 9.66 5.40 11.66
KVVAGVANALSHKYH 10.63 10.13 8.90 10.11 10.56 9.73 8.52 8.95 10.65 11.41 10.27 10.55 9.87 10.19 10.27 10.20 11.87 10.51 10.73 10.08 11.01 10.95 10.69 11.27 10.24 10.19 10.59 8.73 10.77 10.82 10.43 10.34 11.07 11.50 9.63 10.62 10.88
VANALSHKYH 6.82 10.16 9.94 8.94 8.89 8.41 8.12 8.94 10.35 10.00 9.57 9.61 8.30 11.24 9.30 10.12 12.34 8.64 8.78 9.12 10.18 9.93 8.28 11.31 9.43 10.45 11.89 8.28 7.16 10.30 9.31 9.19 9.66 9.51 10.06 9.95 10.80
GRTLYGFGG 9.04 9.76 7.88 9.84 8.93 10.33 9.81 8.72 10.96 8.78 10.88 9.07 8.07 9.73 10.71 8.36 11.55 9.76 8.58 9.01 10.08 8.99 8.99 10.43 10.21 8.38 9.44 9.96 10.06 8.85 8.67 9.80 9.90 10.06 10.31 7.70 8.54
RTLYGFGG 8.94 9.64 7.53 10.04 9.30 10.19 9.09 8.68 11.24 9.58 10.73 8.83 7.92 9.84 10.56 8.25 11.62 9.51 8.68 9.09 10.04 9.14 9.49 10.19 10.67 8.64 9.42 9.86 10.23 8.42 9.34 9.46 9.92 10.46 10.32 8.07 9.19
TLYGFGG 10.40 10.47 10.04 10.00 10.92 12.31 11.63 8.47 12.04 9.87 10.69 6.80 10.59 8.28 12.55 11.14 9.08 10.19 9.47 9.04 9.03 10.04 11.66 9.67 11.17 8.39 11.68 9.99 11.29 8.73 10.21 9.61 9.57 11.41 10.27 8.82 9.98
AADQDLGPEAPPEGVLGA 10.70 8.85 8.19 9.71 11.15 9.59 11.33 10.55 9.03 10.15 9.68 8.30 10.18 7.87 10.22 10.66 10.24 9.61 10.15 9.27 10.35 9.57 9.81 10.21 9.30 10.84 10.48 9.89 9.46 10.57 9.19 10.85 10.06 8.62 10.54 9.55 10.44
AADQDLGPEAPPEGVLGAL 13.86 9.63 8.33 12.24 13.66 11.60 13.35 11.42 10.15 12.63 10.30 9.34 8.68 10.09 10.28 12.73 10.73 12.66 10.46 8.38 12.18 9.91 11.02 12.94 12.56 12.86 11.73 9.82 11.66 12.90 11.12 14.14 11.95 13.05 11.87 10.32 10.48
LENPQPQAPARRLLPP 10.27 8.56 8.87 9.43 9.59 9.71 9.61 8.14 7.39 9.39 9.42 10.16 9.37 9.55 10.99 10.56 9.35 10.48 9.08 9.40 9.68 8.91 9.37 9.74 9.42 10.00 9.29 8.73 9.56 11.04 9.68 9.63 10.47 10.33 7.94 9.74 8.82
VKMALQQEGFD 10.40 8.98 8.99 9.32 11.01 11.65 11.39 9.67 10.31 8.92 8.77 10.22 9.45 9.10 10.49 10.22 10.42 11.74 9.56 9.33 9.83 8.88 9.89 8.43 9.73 10.58 9.48 10.34 9.57 11.40 8.80 10.44 9.64 9.70 8.86 9.28 8.36
GGFMRGL 10.32 9.47 9.31 9.91 7.61 7.92 8.88 11.32 10.47 10.67 10.04 11.12 10.43 7.83 8.36 9.77 9.44 10.95 10.97 9.12 10.53 10.17 8.48 11.38 10.17 8.78 9.87 9.91 7.78 9.36 10.45 10.05 9.98 9.61 10.90 8.92 9.45
Q(-17.03)LEDEAKELQ 9.31 9.27 9.48 9.51 10.50 10.10 6.36 8.35 9.13 10.13 9.88 10.56 9.99 8.54 12.50 9.33 10.18 10.57 9.26 9.57 11.04 9.99 11.50 9.88 10.57 9.83 9.92 9.85 9.49 10.22 9.90 9.95 10.14 10.38 8.35 9.73 11.29
SPQLEDE 8.76 8.99 9.16 10.09 8.10 8.52 9.50 8.16 10.24 9.82 10.48 4.40 8.86 7.88 13.58 9.23 9.74 8.30 8.34 9.37 9.48 10.58 11.90 9.68 10.65 10.19 10.34 10.31 9.98 9.25 9.86 9.71 10.14 8.76 10.24 10.48 10.51
SPQLEDEAKEL 8.69 8.05 8.94 9.11 8.55 9.78 9.72 7.77 9.33 11.18 9.86 5.96 8.08 8.97 10.85 9.59 9.84 9.58 9.27 9.33 10.07 8.77 6.20 10.15 8.80 9.08 9.83 8.67 9.46 9.91 10.02 9.76 9.55 10.31 10.44 9.72 11.10
SPQLEDEAKELQ 7.62 8.61 9.13 9.45 9.88 9.60 8.70 7.43 8.88 10.07 9.53 10.23 9.36 8.37 9.51 8.93 9.59 9.32 10.02 9.23 9.68 9.57 10.78 9.69 9.95 8.61 9.48 8.69 8.95 9.09 9.08 8.50 9.65 9.62 8.90 9.10 9.90
VGRPEWWMDYQ 10.55 8.96 9.55 10.51 10.51 9.83 10.30 8.89 9.78 11.31 11.58 6.77 10.02 7.58 8.87 12.10 9.46 9.04 8.35 9.55 10.40 10.17 11.43 10.15 10.56 10.03 10.06 9.43 10.51 10.37 10.50 10.86 9.89 10.06 11.11 9.41 10.65
YGGFM(+15.99)RF 8.04 9.29 8.63 11.12 11.02 10.56 9.45 8.68 10.01 9.15 10.97 11.15 8.69 8.73 9.70 7.24 10.29 9.26 10.54 11.10 10.52 10.01 12.12 9.54 10.91 9.95 10.38 10.00 10.84 9.21 10.91 10.36 10.05 9.97 10.15 9.30 10.43
YGGFM(+15.99)RGL 8.32 9.67 9.45 10.92 11.12 8.84 9.15 7.68 9.97 9.93 11.03 11.08 9.30 8.44 8.90 12.50 10.52 9.87 10.43 10.68 10.14 10.30 11.85 10.54 11.10 10.05 10.44 10.22 10.34 9.37 10.80 10.32 10.71 9.98 10.48 10.14 10.70
YGGFMRF 9.41 7.74 9.04 8.62 9.21 8.86 9.37 8.17 8.63 10.46 9.57 6.77 8.70 8.13 10.57 8.73 9.42 8.56 8.44 9.46 8.87 7.98 6.59 9.92 8.41 9.23 10.10 7.86 9.38 8.77 9.12 9.40 8.73 9.51 9.52 8.67 9.49
YGGFMRGL 9.58 7.73 9.10 8.68 8.00 9.33 9.35 7.20 8.01 10.57 9.23 6.40 8.01 8.73 10.82 8.29 9.25 8.78 8.67 9.22 9.95 8.79 6.82 9.39 8.10 9.25 10.29 8.25 9.41 9.81 9.52 9.05 9.00 10.65 8.87 8.92 8.91
EIGDEENSAKFPI(-.98) 9.43 9.32 9.19 8.77 10.72 10.47 10.61 10.55 10.89 10.39 9.92 8.33 8.81 10.36 8.18 9.35 9.91 9.73 10.86 9.04 11.04 8.97 7.68 8.02 8.97 9.66 10.43 9.69 9.78 8.94 9.11 10.34 10.10 8.91 11.02 10.07 10.41
DDGPYRMEHFRWGSPPKD 9.53 9.77 10.13 8.69 10.35 10.41 11.22 9.39 9.01 11.86 9.40 9.34 10.10 9.03 10.32 9.86 10.39 11.09 9.79 9.11 10.73 10.25 9.59 9.60 7.45 11.06 9.25 10.21 8.91 12.23 7.89 9.99 10.32 9.14 9.26 9.92 8.01
FKNAIIKNAH 7.75 10.97 9.91 9.01 9.45 8.00 11.18 10.41 10.23 9.39 9.83 8.49 10.25 9.97 10.29 10.60 12.02 7.16 9.28 9.19 9.92 9.40 11.57 10.19 9.27 11.75 10.30 11.21 8.62 11.46 9.99 9.39 11.01 8.97 10.37 10.33 10.20
FKNAIIKNAHKKGQ 10.84 10.34 8.90 7.47 8.75 6.20 10.81 9.28 9.79 9.73 11.47 8.53 10.47 9.46 8.73 10.47 7.72 10.16 7.74 9.14 10.24 10.10 10.12 10.37 9.24 10.55 5.73 10.03 8.89 10.37 8.08 9.50 7.94 8.03 10.14 10.49 6.15
GGPEFRDDGAEPGPRE(-.98) 8.95 8.60 8.92 9.79 10.07 9.54 9.13 7.70 9.78 10.55 9.61 9.12 7.43 8.78 10.31 9.56 10.83 10.01 7.96 10.46 10.78 8.63 7.88 9.95 9.26 10.56 9.84 8.67 9.11 9.06 8.03 8.55 10.24 8.36 9.88 8.16 9.42
GPEFRDDGAEPGPRE(-.98) 9.13 8.95 9.07 9.91 10.00 9.80 9.15 7.77 9.80 10.60 10.19 8.73 6.98 8.73 10.26 9.98 10.93 9.70 8.18 9.50 11.26 8.88 8.21 10.68 10.00 10.53 10.27 9.05 10.07 9.09 8.87 8.66 9.89 10.36 10.33 8.57 9.30
KYVMGHFRWD 9.08 9.67 9.42 7.81 11.35 10.66 10.57 9.92 9.46 9.99 7.91 8.90 8.62 8.82 10.09 9.26 10.31 10.77 9.16 9.62 10.31 9.79 10.25 8.23 8.17 10.51 8.52 10.09 7.35 10.84 8.90 9.58 9.63 8.80 8.46 9.70 9.83
KYVMGHFRWDRF(-.98) 8.84 9.29 8.16 7.90 10.64 10.47 10.73 9.35 9.27 9.93 8.64 9.00 8.64 8.25 10.17 9.57 10.55 10.94 9.03 8.16 10.84 9.84 9.70 9.71 6.72 10.64 9.49 9.25 8.49 11.58 7.25 9.37 10.90 8.63 8.30 10.09 7.36
PEFRDDGAEPGPRE(-.98) 7.93 9.52 8.60 10.12 10.22 9.85 9.27 8.16 10.12 10.64 9.24 9.02 7.45 8.55 10.20 9.45 11.20 9.50 8.38 9.86 11.00 9.43 10.63 10.10 10.28 10.29 10.69 8.36 10.09 9.11 8.31 7.95 10.30 10.15 9.55 9.46 9.13
RPVKVYPNGAEDES(+79.97)AESFPLEF 13.73 9.42 9.62 9.05 10.38 11.95 13.33 10.00 9.86 0.60 9.34 9.48 7.84 8.22 10.04 13.09 8.02 11.21 8.60 10.00 8.94 8.76 8.57 8.87 10.24 10.97 9.97 10.04 10.60 11.49 10.11 12.22 8.58 8.48 9.23 7.96 10.02
RPVKVYPNGAEDESAESFPLEF 12.98 8.52 9.15 9.72 12.26 12.35 11.06 10.57 9.09 9.49 9.44 9.46 11.04 7.59 10.32 10.01 8.97 10.97 9.07 10.57 8.64 7.06 8.16 10.59 9.70 9.85 9.28 9.68 8.46 9.96 9.46 11.47 7.70 9.06 9.64 8.42 9.80
S(+42.01)YSMEHFRWGKPV(-.98) 10.87 9.28 9.77 8.82 10.72 10.99 11.03 9.08 8.54 10.37 7.99 9.59 8.94 9.07 10.58 9.53 10.17 11.34 8.93 10.22 9.52 9.13 10.64 9.69 7.66 10.46 9.97 9.68 8.65 11.45 7.66 10.37 10.79 9.91 7.21 10.01 6.35
SQTPLVTLF 11.46 10.70 10.47 11.78 11.29 10.86 11.30 10.57 9.99 10.37 11.34 9.57 10.21 9.36 10.33 9.22 9.45 9.58 9.83 9.90 10.18 8.26 9.60 8.72 11.67 8.71 11.90 11.30 10.22 10.16 9.33 11.49 9.30 9.44 12.12 5.12 10.80
SQTPLVTLFKNAIIKNAHKKGQ 7.03 9.41 8.24 10.67 10.55 10.92 9.28 8.69 9.66 13.77 10.62 7.03 7.33 5.26 7.83 9.61 10.33 10.28 10.29 10.05 10.94 9.16 10.08 11.78 9.26 9.21 10.08 9.50 10.04 8.75 7.78 10.32 10.28 10.06 11.86 9.82 10.28
SYSMEHF 8.22 9.05 10.15 10.02 10.17 10.04 9.41 10.25 9.74 10.28 10.28 9.73 9.03 9.55 10.35 8.39 10.28 9.90 9.62 10.08 10.47 9.54 10.00 10.28 10.89 8.05 9.59 10.93 9.77 9.47 10.02 10.75 9.59 11.00 10.56 9.16 11.12
SYSMEHFRWGKPV(-.98) 10.25 8.65 9.41 6.81 10.49 10.23 8.77 8.70 7.17 10.92 7.51 10.18 9.55 8.78 10.56 9.98 10.41 10.85 9.84 9.57 10.44 9.80 9.52 9.25 7.97 9.06 9.22 8.14 8.53 9.74 8.49 8.74 10.11 11.67 6.39 10.09 9.11
TPLVTLFKNAIIKNAHKKGQ 5.49 6.33 8.92 10.62 11.12 10.03 8.78 5.03 9.27 10.32 8.51 9.26 9.87 6.54 9.21 10.26 9.20 7.14 7.93 7.96 11.62 7.83 11.03 12.91 10.26 11.18 9.15 8.23 9.89 8.53 9.68 8.95 9.35 9.79 9.96 10.52 10.96
Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLFKNAIIKNAH 13.60 9.79 8.07 8.14 10.95 12.35 12.29 9.10 10.40 9.16 8.08 8.63 11.12 11.23 11.38 11.47 10.16 12.15 10.17 10.10 8.93 9.96 10.06 9.48 8.54 12.38 10.69 9.87 8.75 11.72 8.63 12.56 10.37 9.53 7.81 9.77 8.70
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNA 13.17 7.85 10.45 5.03 13.14 12.19 13.71 9.35 9.62 9.53 10.08 6.99 8.33 10.42 6.95 12.09 8.38 11.82 8.96 8.83 8.44 9.07 10.13 9.22 8.91 12.97 8.49 9.31 7.51 11.96 7.03 12.85 9.86 9.21 8.71 8.67 8.88
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAH 13.53 9.06 9.16 8.37 11.15 11.55 11.86 9.34 8.94 9.33 7.70 9.20 8.74 8.56 10.98 10.91 10.32 12.48 9.37 9.49 9.47 9.72 11.20 9.54 8.24 12.64 10.92 9.99 8.32 12.65 7.72 11.56 10.74 9.94 6.90 9.54 6.06
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAHKKGQ 10.98 10.77 9.21 8.44 7.65 11.07 9.58 10.21 8.21 9.69 8.90 8.19 9.64 9.19 10.89 9.12 9.48 11.53 9.93 8.05 9.57 10.68 10.73 8.36 7.92 10.12 10.40 9.09 7.25 12.06 8.22 10.81 10.57 8.98 7.43 10.08 7.44
YGGFM(+15.99)TSEKSQTPLVTLFKNAIIKNAHKKGQ 10.19 11.01 8.97 10.59 11.23 9.81 9.08 9.91 9.62 10.03 10.15 8.21 7.75 9.37 9.21 10.17 10.89 9.45 10.54 10.76 9.53 10.28 8.73 10.92 10.70 10.15 12.09 10.46 10.33 9.42 9.37 10.80 6.21 11.01 11.61 9.98 10.91
YGGFMTSE 9.50 8.95 9.81 10.40 10.61 10.33 9.85 10.29 9.29 11.65 9.88 8.99 9.42 9.95 10.04 9.32 9.96 10.51 10.10 9.58 10.46 9.87 9.78 9.93 10.74 8.43 11.22 11.05 9.39 10.27 9.32 11.74 9.38 11.09 10.69 8.74 10.61
YGGFMTSEKSQTPLVTLFKNAIIKNA 11.31 10.04 9.86 11.01 11.95 11.57 11.61 10.58 10.42 11.26 10.33 10.11 8.48 8.73 10.33 12.60 10.30 13.05 11.12 9.42 11.72 10.81 9.86 10.67 11.61 12.32 11.20 10.74 11.08 11.50 10.86 11.98 10.08 11.86 11.49 10.66 10.53
YGGFMTSEKSQTPLVTLFKNAIIKNAH 9.74 10.83 10.42 10.11 11.13 10.72 10.24 10.06 9.15 10.09 8.50 8.96 10.34 8.99 10.71 11.76 7.11 11.70 9.62 8.30 10.52 10.11 10.50 7.74 10.21 10.66 9.29 10.92 10.00 10.36 9.54 11.17 8.29 9.35 9.90 10.67 9.92
YGGFMTSEKSQTPLVTLFKNAIIKNAHKKGQ 9.08 9.87 9.57 9.78 10.70 9.72 9.28 10.52 10.41 10.68 10.38 9.70 10.52 9.23 9.92 9.16 10.16 9.16 9.45 9.37 10.31 9.67 9.49 10.76 9.00 9.43 11.31 9.23 9.18 9.12 9.23 9.98 10.22 8.80 10.50 9.24 10.15
ATLESVFQELGKLTGPSSQKRE 11.86 10.14 9.76 11.32 10.20 11.39 10.67 9.42 10.27 11.08 11.41 11.01 11.84 8.15 11.36 9.67 10.10 11.00 11.83 10.28 10.41 11.82 9.91 11.39 10.91 7.84 10.99 6.70 10.32 7.80 12.13 9.09 9.79 10.65 10.39 10.13 10.66
MYEENSRDNPF 8.81 9.00 9.68 9.45 9.51 9.35 9.51 9.54 10.29 10.08 9.57 9.95 10.01 9.22 10.04 8.03 10.07 9.27 10.04 9.05 9.83 9.58 10.14 9.42 10.38 7.96 9.21 9.96 10.12 8.44 10.38 9.84 9.37 11.11 9.65 9.06 9.47
TNEIVEEQYTPQSL 11.93 10.78 11.78 10.97 10.11 10.00 9.82 9.50 11.55 11.06 9.85 8.90 11.40 9.64 10.11 9.43 10.01 7.59 10.78 10.45 9.95 10.14 11.01 10.71 9.99 10.19 12.53 9.12 11.26 8.23 12.16 9.51 9.99 11.42 10.17 10.97 9.78
FPTLGGSQDKSLHN 9.39 8.34 9.08 9.42 9.28 8.70 8.94 9.43 9.31 10.32 9.50 9.59 9.37 9.01 10.40 9.13 10.58 9.38 9.68 9.76 10.54 9.80 9.70 9.94 10.11 9.13 10.55 9.47 9.23 9.51 9.48 8.93 10.06 10.24 9.41 9.32 9.89
TLGGSQDKSLHN 4.15 9.63 9.24 9.25 6.84 7.13 8.97 8.97 11.01 9.99 9.55 9.76 10.10 8.81 10.78 9.12 10.79 7.86 7.44 8.11 10.55 10.42 10.41 10.02 10.29 9.29 10.48 9.68 9.56 8.21 9.50 7.92 10.46 10.22 10.39 7.25 9.53
SVNPYLQGQRL 9.56 9.13 9.30 7.66 6.05 8.24 6.46 9.64 9.92 9.01 7.88 9.35 9.39 8.79 10.51 6.71 10.55 7.48 8.62 8.51 10.48 8.94 9.32 9.52 9.12 8.77 10.50 8.22 9.01 7.74 9.90 6.34 9.56 11.99 7.13 9.53 7.65
SVNPYLQGQRLDNVV 11.80 9.16 10.22 11.75 11.79 11.82 11.58 9.17 9.83 10.10 10.67 9.22 5.50 9.12 8.75 10.68 9.60 11.57 10.14 9.94 11.31 8.72 8.51 11.21 11.39 11.74 11.66 10.62 10.99 11.46 10.10 11.64 9.82 11.70 11.70 9.05 9.49
SVNPYLQGQRLDNVVA 11.48 9.09 9.61 11.33 11.05 10.94 11.37 9.36 9.82 10.93 10.51 10.17 9.55 8.96 10.54 10.36 10.42 10.63 10.05 9.59 11.62 9.31 9.31 11.66 10.80 10.82 11.59 10.21 10.69 11.10 10.05 11.28 10.31 11.22 11.14 8.39 9.75
SANSNPAM(+15.99)APRE 9.53 9.52 10.10 10.89 9.40 5.78 8.35 7.24 11.31 9.96 10.97 11.41 7.66 6.31 5.69 8.40 10.01 7.01 11.40 9.13 11.75 10.58 11.54 11.56 10.53 9.92 8.24 8.64 11.14 7.86 10.71 6.40 10.04 9.36 11.44 9.60 8.78
SANSNPAMAPRE 8.03 8.08 9.65 9.23 8.10 6.83 9.37 7.41 9.75 11.41 9.30 6.97 7.32 6.66 10.67 8.98 10.23 8.19 8.53 9.77 10.95 8.45 6.50 10.74 8.70 9.85 9.60 9.14 10.02 8.63 9.62 7.37 9.29 10.19 10.77 8.86 9.18

EigenMS removes sample-to-sample variability.

Univariate analysis

Univariate statistic analysis will proceed for each pairs of comparison. For each pair,

Step 1, differential analysis;

  • t-test

  • Wilcoxon rank-sum test

For all statistical tests, the Benjamini-Hochberg (BH) procedure was applied to correct for multiple hypothesis testing.

Step 2, fold change, both linear and log2;

Step 3, regulation shown in volcano plot.

  • A feature is considered unregulated when FC(log2) > 0.6 (or FC(lin) > 1.5);

  • A feature is considered downregulated when FC(log2) < -0.6 (or FC(lin) < -1.5).

Code
d5 <- rbind.data.frame(labels_d1, norm_d1_mod) 
d5_mod <- t(d5) %>%
  as.data.frame(.) %>%
  rownames_to_column(., var = "rowname") %>%
  as_tibble() %>%
  dplyr::rename(., Label = class) %>%
  mutate(across(-c(Label,rowname), as.numeric)) %>%
  #rename_with(str_trim)
  column_to_rownames(., var = "rowname")

#colnames(d5_mod)[-1] == feature_name$variable
colnames(d5_mod)[-1] = feature_name$name

grps = as.factor(t(labels_d1))
trt= levels(grps)

combo_list = do.call("c", lapply(seq_along(time), function(i) combn(trt, 2, FUN = list))) # top 10 are unique combo
#combo_list = combo_list[1:10]

feature_meta = read.csv(file = "feature_meta_pituitary.csv")

folder = paste(getwd(), "/pituitary/", sep = "")
Code
contrast = combo_list[[1]]

df = d5_mod %>%
    dplyr::filter(., Label %in% contrast ) %>%
    droplevels()

temp_d <- df %>%
        mutate(across(-Label, ~ 2^(.) )) %>% # d3_mod is log-scale number, this step will de-log and change data back to linear number
        group_by(Label) %>%
        summarise(across(everything(), mean))

print(paste("Order of Fold Change:", temp_d$Label[1], "over", temp_d$Label[2], sep = " "))
[1] "Order of Fold Change: August over IBA"
Code
uni_res <- do_univariate(df)

uni_res <- uni_res %>%
        rowwise() %>%
        mutate(padj = min(c(BHT, BHW))) %>%
        # get the lowest padj
        ungroup() %>%
        mutate(`-log10padj` = -log10(padj))

uni_res_annotation = uni_res %>% left_join(., feature_meta, by = c("variable" = "Peptide"))

uni_res_annotation %>% 
        knitr::kable(., digits = 3, "html") %>%
        kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
        kableExtra::scroll_box(width = "100%", height = "500px")
variable pT BHT pW BHW FC(lin) FC(log2) padj -log10padj Accession Name GN
AADQDLGPEAPPEGVLGA 0.012 0.035 0.012 0.035 0.482 -1.053 0.035 1.454 tr|I3NGD6|I3NGD6_ICTTR ProSAAS PCSK1N
AADQDLGPEAPPEGVLGAL 0.000 0.001 0.000 0.003 0.119 -3.072 0.001 2.966 tr|I3NGD6|I3NGD6_ICTTR ProSAAS PCSK1N
AAMDLEL 0.592 0.704 0.456 0.579 0.430 -1.218 0.579 0.237 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
AELDQLLHY 0.561 0.676 0.529 0.654 0.635 -0.654 0.654 0.184 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
AFGPRGPGLQL 0.134 0.233 0.224 0.351 0.609 -0.716 0.233 0.633 tr|I3MMW2|I3MMW2_ICTTR Chromogranin-A CHGA
AGAPEPAEPMELAKPSA 0.005 0.019 0.002 0.008 0.269 -1.893 0.008 2.102 tr|I3MXH3|I3MXH3_ICTTR Vasopressin-neurophysin 2-copeptin-like AVP
ATLESVFQELGKLTGPSSQKRE 0.934 0.954 0.955 0.955 1.184 0.244 0.954 0.021 tr|I3N6V4|I3N6V4_ICTTR Secretogranin-2 SCG2
DDGPYRMEHFRWGSPPKD 0.053 0.122 0.113 0.213 0.546 -0.872 0.122 0.915 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
EDREEASLQDRQYASHHT 0.010 0.030 0.008 0.025 2.267 1.181 0.025 1.609 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EDREEASLQDRQYASHHTTE 0.011 0.031 0.018 0.047 1.935 0.952 0.031 1.503 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EEASLQDRQYASHHT 0.000 0.001 0.001 0.006 4.550 2.186 0.001 3.194 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EEASLQDRQYASHHTTE 0.000 0.002 0.002 0.008 2.043 1.030 0.002 2.626 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EESEESEEGTTSEVT 0.002 0.011 0.002 0.008 3.628 1.859 0.008 2.102 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EIGDEENSAKFPI(-.98) 0.331 0.472 0.456 0.579 0.799 -0.323 0.472 0.326 tr|I3MQ49|I3MQ49_ICTTR Pro-melanin concentrating hormone PMCH
ELDQLLHY 0.229 0.341 0.328 0.467 1.289 0.367 0.341 0.467 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FKNAIIKNAH 0.366 0.514 0.607 0.713 1.108 0.148 0.514 0.289 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
FKNAIIKNAHKKGQ 0.795 0.839 0.328 0.467 0.790 -0.340 0.467 0.331 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
FLGEAYHHVPE 0.065 0.139 0.113 0.213 1.831 0.872 0.139 0.857 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPES 0.108 0.198 0.113 0.213 1.515 0.599 0.198 0.702 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPESQ 0.059 0.128 0.088 0.188 1.770 0.824 0.128 0.893 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPESQRD 0.003 0.012 0.008 0.025 1.530 0.613 0.012 1.924 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPESQRDKA 0.003 0.013 0.000 0.003 2.422 1.276 0.003 2.467 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FPTLGGSQDKSLHN 0.031 0.082 0.050 0.119 1.278 0.353 0.082 1.089 tr|I3MPL6|I3MPL6_ICTTR Secretogranin-3 SCG3
GEAYHHVPESQRD 0.000 0.003 0.002 0.008 3.477 1.798 0.003 2.580 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
GEAYHHVPESQRDKA 0.005 0.019 0.003 0.011 3.990 1.996 0.011 1.942 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
GGFMRGL 0.533 0.658 0.607 0.713 1.167 0.223 0.658 0.181 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
GGPEFRDDGAEPGPRE(-.98) 0.001 0.007 0.003 0.011 0.437 -1.195 0.007 2.138 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
GPEFRDDGAEPGPRE(-.98) 0.004 0.017 0.003 0.011 0.514 -0.961 0.011 1.942 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
GRTLYGFGG 0.197 0.313 0.328 0.467 0.699 -0.516 0.313 0.504 tr|I3N7M0|I3N7M0_ICTTR Histone H4 LOC106145262
GVANALSHKYH 0.441 0.583 0.864 0.902 0.840 -0.251 0.583 0.235 tr|A0A287CUK4|A0A287CUK4_ICTTR Hemoglobin subunit beta-S/F-like LOC101967841
GVLFNPYYDPLQWKSSRFE 0.070 0.146 0.145 0.252 0.412 -1.280 0.146 0.836 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
KVVAGVANALSHKYH 0.893 0.933 0.456 0.579 1.042 0.059 0.579 0.237 tr|A0A287CUK4|A0A287CUK4_ICTTR Hemoglobin subunit beta-S/F-like LOC101967841
KYVMGHFRWD 0.097 0.188 0.181 0.304 0.623 -0.682 0.188 0.727 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
KYVMGHFRWDRF(-.98) 0.035 0.088 0.066 0.152 0.483 -1.051 0.088 1.055 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
LAGAPEPA 0.036 0.090 0.036 0.089 0.235 -2.089 0.089 1.051 tr|I3MXH3|I3MXH3_ICTTR Vasopressin-neurophysin 2-copeptin-like AVP
LDQLLHY 0.000 0.003 0.000 0.003 2.350 1.233 0.003 2.580 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
LENPQPQAPARRLLPP 0.001 0.006 0.005 0.018 0.491 -1.025 0.006 2.224 tr|I3NGD6|I3NGD6_ICTTR ProSAAS PCSK1N
LGEAYHHVPESQRD 0.039 0.092 0.066 0.152 1.922 0.943 0.092 1.036 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
LGVLFNPYYDPLQWKSSRFE 0.000 0.003 0.003 0.011 0.156 -2.680 0.003 2.580 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
MYEENSRDNPF 0.016 0.045 0.018 0.047 1.605 0.682 0.045 1.346 tr|I3N6V4|I3N6V4_ICTTR Secretogranin-3 SCG2
PEFRDDGAEPGPRE(-.98) 0.758 0.829 0.776 0.848 0.935 -0.097 0.829 0.082 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Q(-17.03)EPGAPAAGM 0.141 0.236 0.181 0.304 0.737 -0.440 0.236 0.627 tr|I3MIV6|I3MIV6_ICTTR Carboxypeptidase E CPE
Q(-17.03)HWSYGLRPG(-.98) 0.055 0.123 0.018 0.047 0.466 -1.103 0.047 1.326 tr|I3MCA0|I3MCA0_ICTTR Progonadoliberin-1 GNRH1
Q(-17.03)KIAEKFSQ 0.007 0.022 0.012 0.035 2.551 1.351 0.022 1.652 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
Q(-17.03)KIAEKFSQR(-.98) 0.006 0.021 0.008 0.025 3.821 1.934 0.021 1.683 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
Q(-17.03)LEDEAKELQ 0.474 0.611 0.864 0.902 1.237 0.307 0.611 0.214 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
Q(-17.03)YDRVAELDQLLHY 0.000 0.000 0.000 0.003 0.066 -3.912 0.000 3.769 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
QYDRVAELDQLLHY 0.030 0.081 0.026 0.067 0.516 -0.956 0.067 1.175 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
RPVKVYPNGAEDES(+79.97)AESFPLEF 0.000 0.001 0.000 0.003 0.099 -3.337 0.001 3.194 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
RPVKVYPNGAEDESAESFPLEF 0.000 0.003 0.000 0.003 0.143 -2.801 0.003 2.580 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
RTLYGFGG 0.494 0.627 0.689 0.771 0.867 -0.206 0.627 0.202 tr|I3N7M0|I3N7M0_ICTTR Histone H4 LOC106145262
RVLTEEEEKELENL 0.416 0.559 0.607 0.713 1.027 0.039 0.559 0.253 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
S(+42.01)YSMEHFRWGKPV(-.98) 0.007 0.022 0.012 0.035 0.486 -1.041 0.022 1.652 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
SANSNPAM(+15.99)APRE 0.005 0.019 0.005 0.018 4.009 2.003 0.018 1.744 tr|I3MP38|I3MP38_ICTTR Somatostatin SST
SANSNPAMAPRE 0.790 0.839 0.689 0.771 1.103 0.142 0.771 0.113 tr|I3MP38|I3MP38_ICTTR Somatostatin SST
SLDSPAGPA 0.938 0.954 0.955 0.955 0.832 -0.265 0.954 0.021 tr|I3M5J1|I3M5J1_ICTTR Corticoliberin CRH
SLDSPAGPAE 0.236 0.346 0.224 0.351 3.406 1.768 0.346 0.460 tr|I3M5J1|I3M5J1_ICTTR Corticoliberin CRH
SPQLEDE 0.096 0.188 0.088 0.188 2.513 1.329 0.188 0.727 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
SPQLEDEAKEL 0.176 0.286 0.145 0.252 0.671 -0.575 0.252 0.599 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
SPQLEDEAKELQ 0.129 0.228 0.145 0.252 1.600 0.678 0.228 0.641 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
SQGGEPGAYLTPDT 0.104 0.195 0.224 0.351 1.377 0.462 0.195 0.710 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
SQGGEPGAYLTPDTREE 0.666 0.764 0.955 0.955 1.055 0.078 0.764 0.117 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
SQTPLVTLF 0.204 0.320 0.272 0.406 0.577 -0.794 0.320 0.495 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
SQTPLVTLFKNAIIKNAHKKGQ 0.944 0.954 0.689 0.771 0.853 -0.230 0.771 0.113 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
SSQEGNPPSEERGHTLQEPEEAKVGE 0.640 0.742 0.864 0.902 1.171 0.228 0.742 0.129 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
SVNPYLQGQRL 0.006 0.021 0.036 0.089 2.152 1.106 0.021 1.683 tr|I3MEQ9|I3MEQ9_ICTTR Neuroendocrine protein 7B2 SCG5
SVNPYLQGQRLDNVV 0.002 0.009 0.000 0.003 0.281 -1.833 0.003 2.467 tr|I3MEQ9|I3MEQ9_ICTTR Neuroendocrine protein 7B3 SCG5
SVNPYLQGQRLDNVVA 0.001 0.007 0.000 0.003 0.366 -1.448 0.003 2.467 tr|I3MEQ9|I3MEQ9_ICTTR Neuroendocrine protein 7B4 SCG5
SVSTVLTSKYR 0.786 0.839 0.388 0.529 1.166 0.221 0.529 0.276 tr|A0A287D1R9|A0A287D1R9_ICTTR Hemoglobin subunit alpha LOC101956162
SYSMEHF 0.219 0.332 0.388 0.529 1.302 0.380 0.332 0.479 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
SYSMEHFRWGKPV(-.98) 0.414 0.559 0.388 0.529 0.769 -0.379 0.529 0.276 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
TLGGSQDKSLHN 0.110 0.199 0.113 0.213 2.468 1.303 0.199 0.701 tr|I3MPL6|I3MPL6_ICTTR Secretogranin-4 SCG3
TLYGFGG 0.539 0.658 0.529 0.654 0.684 -0.548 0.654 0.184 tr|I3N7M0|I3N7M0_ICTTR Histone H4 LOC106145262
TNEIVEEQYTPQSL 0.098 0.188 0.113 0.213 1.656 0.728 0.188 0.727 tr|I3N6V4|I3N6V4_ICTTR Secretogranin-4 SCG2
TPLVTLFKNAIIKNAHKKGQ 0.978 0.978 0.776 0.848 0.869 -0.203 0.848 0.072 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
VAELDQLLHY 0.084 0.171 0.088 0.188 0.633 -0.659 0.171 0.766 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
VANALSHKYH 0.708 0.783 0.955 0.955 1.007 0.010 0.783 0.106 tr|A0A287CUK4|A0A287CUK4_ICTTR Hemoglobin subunit beta-S/F-like LOC101967841
VGRPEWWMDYQ 0.209 0.322 0.145 0.252 0.668 -0.582 0.252 0.599 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
VKMALQQEGFD 0.000 0.003 0.001 0.006 0.353 -1.501 0.003 2.580 tr|I3MEG8|I3MEG8_ICTTR Proprotein convertase subtilisin/kexin type 2 PCSK2
VKVGVNGF 0.446 0.583 0.456 0.579 0.808 -0.307 0.579 0.237 tr|A0A287D887|A0A287D887_ICTTR Glyceraldehyde-3-phosphate dehydrogenase GAPDH
Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLFKNAIIKNAH 0.000 0.001 0.000 0.003 0.170 -2.554 0.001 2.857 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNA 0.000 0.000 0.000 0.003 0.108 -3.208 0.000 3.769 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAH 0.000 0.002 0.002 0.008 0.209 -2.255 0.002 2.654 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAHKKGQ 0.502 0.629 0.272 0.406 0.619 -0.692 0.406 0.391 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
YDRVAELDQLLHY 0.006 0.021 0.002 0.008 0.336 -1.574 0.008 2.102 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
YGGFM(+15.99)RF 0.329 0.472 0.456 0.579 1.650 0.723 0.472 0.326 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
YGGFM(+15.99)RGL 0.411 0.559 0.272 0.406 1.058 0.081 0.406 0.391 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
YGGFM(+15.99)TSEKSQTPLVTLFKNAIIKNAHKKGQ 0.612 0.720 0.864 0.902 0.871 -0.199 0.720 0.143 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
YGGFMRF 0.039 0.092 0.008 0.025 0.569 -0.815 0.025 1.609 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
YGGFMRGL 0.150 0.247 0.113 0.213 0.674 -0.569 0.213 0.671 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
YGGFMTSE 0.704 0.783 0.689 0.771 0.823 -0.281 0.771 0.113 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
YGGFMTSEKSQTPLVTLFKNAIIKNA 0.000 0.003 0.000 0.003 0.350 -1.513 0.003 2.580 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
YGGFMTSEKSQTPLVTLFKNAIIKNAH 0.139 0.236 0.224 0.351 0.688 -0.539 0.236 0.627 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
YGGFMTSEKSQTPLVTLFKNAIIKNAHKKGQ 0.707 0.783 0.607 0.713 0.889 -0.169 0.713 0.147 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Code
# get DE features only tibble
uni_res_filt <- uni_res %>%
        dplyr::filter(BHT < FDR & BHW < FDR) %>%
        mutate(status = case_when(`FC(log2)` < - 0.6 ~ "Down",
                                  `FC(log2)` > 0.6 ~ "Up") )

if (nrow(uni_res_filt) == 0){
        print(paste("There is no significant differentiated features between",
                    temp_d$Label[2], "and", temp_d$Label[1], sep = " "))
} else {
        uni_res_filt = uni_res_filt %>%
                left_join(., feature_meta, by = c("variable" = "Peptide"))
        
        uni_res_filt %>% 
                knitr::kable(., digits = 3, "html") %>%
                kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
                kableExtra::scroll_box(width = "100%", height = "500px")
}
variable pT BHT pW BHW FC(lin) FC(log2) padj -log10padj status Accession Name GN
AADQDLGPEAPPEGVLGA 0.012 0.035 0.012 0.035 0.482 -1.053 0.035 1.454 Down tr|I3NGD6|I3NGD6_ICTTR ProSAAS PCSK1N
AADQDLGPEAPPEGVLGAL 0.000 0.001 0.000 0.003 0.119 -3.072 0.001 2.966 Down tr|I3NGD6|I3NGD6_ICTTR ProSAAS PCSK1N
AGAPEPAEPMELAKPSA 0.005 0.019 0.002 0.008 0.269 -1.893 0.008 2.102 Down tr|I3MXH3|I3MXH3_ICTTR Vasopressin-neurophysin 2-copeptin-like AVP
EDREEASLQDRQYASHHT 0.010 0.030 0.008 0.025 2.267 1.181 0.025 1.609 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EDREEASLQDRQYASHHTTE 0.011 0.031 0.018 0.047 1.935 0.952 0.031 1.503 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EEASLQDRQYASHHT 0.000 0.001 0.001 0.006 4.550 2.186 0.001 3.194 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EEASLQDRQYASHHTTE 0.000 0.002 0.002 0.008 2.043 1.030 0.002 2.626 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EESEESEEGTTSEVT 0.002 0.011 0.002 0.008 3.628 1.859 0.008 2.102 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPESQRD 0.003 0.012 0.008 0.025 1.530 0.613 0.012 1.924 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPESQRDKA 0.003 0.013 0.000 0.003 2.422 1.276 0.003 2.467 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
GEAYHHVPESQRD 0.000 0.003 0.002 0.008 3.477 1.798 0.003 2.580 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
GEAYHHVPESQRDKA 0.005 0.019 0.003 0.011 3.990 1.996 0.011 1.942 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
GGPEFRDDGAEPGPRE(-.98) 0.001 0.007 0.003 0.011 0.437 -1.195 0.007 2.138 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
GPEFRDDGAEPGPRE(-.98) 0.004 0.017 0.003 0.011 0.514 -0.961 0.011 1.942 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
LDQLLHY 0.000 0.003 0.000 0.003 2.350 1.233 0.003 2.580 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
LENPQPQAPARRLLPP 0.001 0.006 0.005 0.018 0.491 -1.025 0.006 2.224 Down tr|I3NGD6|I3NGD6_ICTTR ProSAAS PCSK1N
LGVLFNPYYDPLQWKSSRFE 0.000 0.003 0.003 0.011 0.156 -2.680 0.003 2.580 Down tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
MYEENSRDNPF 0.016 0.045 0.018 0.047 1.605 0.682 0.045 1.346 Up tr|I3N6V4|I3N6V4_ICTTR Secretogranin-3 SCG2
Q(-17.03)KIAEKFSQ 0.007 0.022 0.012 0.035 2.551 1.351 0.022 1.652 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
Q(-17.03)KIAEKFSQR(-.98) 0.006 0.021 0.008 0.025 3.821 1.934 0.021 1.683 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
Q(-17.03)YDRVAELDQLLHY 0.000 0.000 0.000 0.003 0.066 -3.912 0.000 3.769 Down tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
RPVKVYPNGAEDES(+79.97)AESFPLEF 0.000 0.001 0.000 0.003 0.099 -3.337 0.001 3.194 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
RPVKVYPNGAEDESAESFPLEF 0.000 0.003 0.000 0.003 0.143 -2.801 0.003 2.580 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
S(+42.01)YSMEHFRWGKPV(-.98) 0.007 0.022 0.012 0.035 0.486 -1.041 0.022 1.652 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
SANSNPAM(+15.99)APRE 0.005 0.019 0.005 0.018 4.009 2.003 0.018 1.744 Up tr|I3MP38|I3MP38_ICTTR Somatostatin SST
SVNPYLQGQRLDNVV 0.002 0.009 0.000 0.003 0.281 -1.833 0.003 2.467 Down tr|I3MEQ9|I3MEQ9_ICTTR Neuroendocrine protein 7B3 SCG5
SVNPYLQGQRLDNVVA 0.001 0.007 0.000 0.003 0.366 -1.448 0.003 2.467 Down tr|I3MEQ9|I3MEQ9_ICTTR Neuroendocrine protein 7B4 SCG5
VKMALQQEGFD 0.000 0.003 0.001 0.006 0.353 -1.501 0.003 2.580 Down tr|I3MEG8|I3MEG8_ICTTR Proprotein convertase subtilisin/kexin type 2 PCSK2
Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLFKNAIIKNAH 0.000 0.001 0.000 0.003 0.170 -2.554 0.001 2.857 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNA 0.000 0.000 0.000 0.003 0.108 -3.208 0.000 3.769 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAH 0.000 0.002 0.002 0.008 0.209 -2.255 0.002 2.654 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
YDRVAELDQLLHY 0.006 0.021 0.002 0.008 0.336 -1.574 0.008 2.102 Down tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
YGGFMTSEKSQTPLVTLFKNAIIKNA 0.000 0.003 0.000 0.003 0.350 -1.513 0.003 2.580 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Code
# volcano plot
volcano_plot(uni_res_annotation, feature_col = "variable", fdr = FDR, log2fc = LOG2FC, save = FALSE)

Code
# heatmap
anno <- data.frame(Label = as.factor(t(d5)[, "class"])) %>%
        filter(Label %in% contrast ) %>%
        droplevels()

hm <- d5[-1, d5[1,] %in% contrast ] %>%
        rownames_to_column("variable") %>%
        mutate(across(-variable, as.numeric)) #%>%
        #left_join(., feature_meta, by = c("variable" = "Feature"))

# select color palette of current pair
selected_colors = anno_colors[levels(anno[[1]])]

heatmap(hm, feature_col = "variable", sample_anno = anno, sample_colors = selected_colors, feature_anno = feature_meta, feature_label = "Peptide", rowname_switch = TRUE, save = FALSE)

Code
contrast = combo_list[[2]]

df = d5_mod %>%
    dplyr::filter(., Label %in% contrast ) %>%
    droplevels()

temp_d <- df %>%
        mutate(across(-Label, ~ 2^(.) )) %>% # d3_mod is log-scale number, this step will de-log and change data back to linear number
        group_by(Label) %>%
        summarise(across(everything(), mean))

print(paste("Order of Fold Change:", temp_d$Label[1], "over", temp_d$Label[2], sep = " "))
[1] "Order of Fold Change: August over March"
Code
uni_res <- do_univariate(df)

uni_res <- uni_res %>%
        rowwise() %>%
        mutate(padj = min(c(BHT, BHW))) %>%
        # get the lowest padj
        ungroup() %>%
        mutate(`-log10padj` = -log10(padj))

uni_res_annotation = uni_res %>% left_join(., feature_meta, by = c("variable" = "Peptide"))

uni_res_annotation %>% 
        knitr::kable(., digits = 3, "html") %>%
        kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
        kableExtra::scroll_box(width = "100%", height = "500px")
variable pT BHT pW BHW FC(lin) FC(log2) padj -log10padj Accession Name GN
AADQDLGPEAPPEGVLGA 0.078 0.127 0.036 0.070 0.674 -0.570 0.070 1.152 tr|I3NGD6|I3NGD6_ICTTR ProSAAS PCSK1N
AADQDLGPEAPPEGVLGAL 0.021 0.048 0.036 0.070 0.482 -1.053 0.048 1.321 tr|I3NGD6|I3NGD6_ICTTR ProSAAS PCSK1N
AAMDLEL 0.727 0.781 0.743 0.785 0.624 -0.680 0.781 0.107 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
AELDQLLHY 0.229 0.296 0.370 0.446 1.533 0.617 0.296 0.529 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
AFGPRGPGLQL 0.136 0.196 0.093 0.141 1.409 0.495 0.141 0.852 tr|I3MMW2|I3MMW2_ICTTR Chromogranin-A CHGA
AGAPEPAEPMELAKPSA 0.001 0.006 0.002 0.006 0.248 -2.011 0.006 2.213 tr|I3MXH3|I3MXH3_ICTTR Vasopressin-neurophysin 2-copeptin-like AVP
ATLESVFQELGKLTGPSSQKRE 0.174 0.241 0.370 0.446 1.245 0.316 0.241 0.619 tr|I3N6V4|I3N6V4_ICTTR Secretogranin-2 SCG2
DDGPYRMEHFRWGSPPKD 0.317 0.382 0.277 0.356 0.752 -0.412 0.356 0.448 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
EDREEASLQDRQYASHHT 0.007 0.021 0.021 0.047 3.147 1.654 0.021 1.677 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EDREEASLQDRQYASHHTTE 0.001 0.004 0.001 0.003 2.874 1.523 0.003 2.545 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EEASLQDRQYASHHT 0.000 0.002 0.001 0.003 3.938 1.978 0.002 2.680 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EEASLQDRQYASHHTTE 0.000 0.003 0.001 0.003 2.415 1.272 0.003 2.545 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EESEESEEGTTSEVT 0.278 0.348 0.423 0.498 1.832 0.873 0.348 0.459 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EIGDEENSAKFPI(-.98) 0.522 0.577 0.673 0.727 0.940 -0.089 0.577 0.239 tr|I3MQ49|I3MQ49_ICTTR Pro-melanin concentrating hormone PMCH
ELDQLLHY 0.086 0.138 0.046 0.079 1.957 0.969 0.079 1.101 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FKNAIIKNAH 0.364 0.417 0.606 0.670 1.116 0.158 0.417 0.380 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
FKNAIIKNAHKKGQ 0.133 0.195 0.046 0.079 0.503 -0.991 0.079 1.101 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
FLGEAYHHVPE 0.011 0.027 0.011 0.029 2.349 1.232 0.027 1.563 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPES 0.071 0.119 0.236 0.308 1.548 0.631 0.119 0.923 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPESQ 0.006 0.018 0.001 0.004 2.518 1.332 0.004 2.355 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPESQRD 0.000 0.000 0.000 0.001 4.087 2.031 0.000 4.650 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPESQRDKA 0.000 0.001 0.000 0.001 4.343 2.119 0.001 3.141 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FPTLGGSQDKSLHN 0.000 0.000 0.000 0.001 2.250 1.170 0.000 5.140 tr|I3MPL6|I3MPL6_ICTTR Secretogranin-3 SCG3
GEAYHHVPESQRD 0.001 0.004 0.001 0.004 3.056 1.611 0.004 2.423 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
GEAYHHVPESQRDKA 0.001 0.004 0.000 0.001 4.852 2.279 0.001 3.066 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
GGFMRGL 0.179 0.245 0.236 0.308 1.491 0.576 0.245 0.612 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
GGPEFRDDGAEPGPRE(-.98) 0.101 0.154 0.093 0.141 1.558 0.640 0.141 0.852 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
GPEFRDDGAEPGPRE(-.98) 0.002 0.006 0.002 0.009 1.895 0.923 0.006 2.209 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
GRTLYGFGG 0.036 0.072 0.036 0.070 1.904 0.929 0.070 1.152 tr|I3N7M0|I3N7M0_ICTTR Histone H4 LOC106145262
GVANALSHKYH 0.055 0.102 0.046 0.079 3.878 1.955 0.079 1.101 tr|A0A287CUK4|A0A287CUK4_ICTTR Hemoglobin subunit beta-S/F-like LOC101967841
GVLFNPYYDPLQWKSSRFE 0.873 0.922 0.815 0.851 0.839 -0.253 0.851 0.070 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
KVVAGVANALSHKYH 0.006 0.018 0.004 0.012 1.886 0.916 0.012 1.936 tr|A0A287CUK4|A0A287CUK4_ICTTR Hemoglobin subunit beta-S/F-like LOC101967841
KYVMGHFRWD 0.061 0.108 0.046 0.079 0.580 -0.787 0.079 1.101 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
KYVMGHFRWDRF(-.98) 0.489 0.553 0.541 0.606 0.794 -0.333 0.553 0.257 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
LAGAPEPA 0.000 0.001 0.000 0.001 0.043 -4.541 0.001 3.141 tr|I3MXH3|I3MXH3_ICTTR Vasopressin-neurophysin 2-copeptin-like AVP
LDQLLHY 0.000 0.000 0.000 0.001 4.580 2.195 0.000 3.902 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
LENPQPQAPARRLLPP 0.499 0.559 0.423 0.498 0.898 -0.156 0.498 0.303 tr|I3NGD6|I3NGD6_ICTTR ProSAAS PCSK1N
LGEAYHHVPESQRD 0.000 0.002 0.000 0.001 5.558 2.475 0.001 3.066 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
LGVLFNPYYDPLQWKSSRFE 0.070 0.119 0.059 0.098 0.536 -0.901 0.098 1.010 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
MYEENSRDNPF 0.010 0.026 0.021 0.047 1.880 0.911 0.026 1.581 tr|I3N6V4|I3N6V4_ICTTR Secretogranin-3 SCG2
PEFRDDGAEPGPRE(-.98) 0.002 0.009 0.002 0.009 2.148 1.103 0.009 2.066 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Q(-17.03)EPGAPAAGM 0.294 0.363 0.167 0.238 0.804 -0.316 0.238 0.623 tr|I3MIV6|I3MIV6_ICTTR Carboxypeptidase E CPE
Q(-17.03)HWSYGLRPG(-.98) 0.001 0.005 0.006 0.015 2.119 1.084 0.005 2.268 tr|I3MCA0|I3MCA0_ICTTR Progonadoliberin-1 GNRH1
Q(-17.03)KIAEKFSQ 0.003 0.010 0.001 0.003 3.025 1.597 0.003 2.545 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
Q(-17.03)KIAEKFSQR(-.98) 0.001 0.004 0.000 0.001 5.746 2.523 0.001 2.890 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
Q(-17.03)LEDEAKELQ 0.095 0.147 0.167 0.238 1.922 0.942 0.147 0.833 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
Q(-17.03)YDRVAELDQLLHY 0.045 0.087 0.046 0.079 0.418 -1.258 0.079 1.101 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
QYDRVAELDQLLHY 0.214 0.283 0.167 0.238 0.688 -0.540 0.238 0.623 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
RPVKVYPNGAEDES(+79.97)AESFPLEF 0.006 0.019 0.000 0.001 0.091 -3.466 0.001 3.066 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
RPVKVYPNGAEDESAESFPLEF 0.001 0.006 0.002 0.009 0.232 -2.109 0.006 2.213 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
RTLYGFGG 0.003 0.010 0.004 0.012 2.269 1.182 0.010 1.987 tr|I3N7M0|I3N7M0_ICTTR Histone H4 LOC106145262
RVLTEEEEKELENL 0.936 0.966 0.888 0.898 0.933 -0.100 0.898 0.047 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
S(+42.01)YSMEHFRWGKPV(-.98) 0.023 0.052 0.021 0.047 0.633 -0.661 0.047 1.325 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
SANSNPAM(+15.99)APRE 0.095 0.147 0.074 0.119 3.343 1.741 0.119 0.926 tr|I3MP38|I3MP38_ICTTR Somatostatin SST
SANSNPAMAPRE 0.000 0.002 0.001 0.003 3.701 1.888 0.002 2.796 tr|I3MP38|I3MP38_ICTTR Somatostatin SST
SLDSPAGPA 0.968 0.978 0.743 0.785 1.050 0.071 0.785 0.105 tr|I3M5J1|I3M5J1_ICTTR Corticoliberin CRH
SLDSPAGPAE 0.003 0.010 0.004 0.012 3.484 1.801 0.010 1.987 tr|I3M5J1|I3M5J1_ICTTR Corticoliberin CRH
SPQLEDE 0.073 0.120 0.036 0.070 3.874 1.954 0.070 1.152 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
SPQLEDEAKEL 0.007 0.019 0.006 0.015 1.794 0.843 0.015 1.817 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
SPQLEDEAKELQ 0.010 0.026 0.011 0.029 1.542 0.625 0.026 1.581 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
SQGGEPGAYLTPDT 0.056 0.102 0.074 0.119 1.755 0.812 0.102 0.990 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
SQGGEPGAYLTPDTREE 0.010 0.026 0.002 0.009 1.626 0.701 0.009 2.066 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
SQTPLVTLF 0.335 0.394 0.481 0.544 0.728 -0.458 0.394 0.404 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
SQTPLVTLFKNAIIKNAHKKGQ 0.155 0.221 0.200 0.272 3.215 1.685 0.221 0.656 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
SSQEGNPPSEERGHTLQEPEEAKVGE 0.113 0.168 0.236 0.308 1.409 0.494 0.168 0.774 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
SVNPYLQGQRL 0.000 0.001 0.001 0.003 6.020 2.590 0.001 3.031 tr|I3MEQ9|I3MEQ9_ICTTR Neuroendocrine protein 7B2 SCG5
SVNPYLQGQRLDNVV 0.032 0.068 0.021 0.047 0.593 -0.755 0.047 1.325 tr|I3MEQ9|I3MEQ9_ICTTR Neuroendocrine protein 7B3 SCG5
SVNPYLQGQRLDNVVA 0.893 0.932 0.888 0.898 1.062 0.087 0.898 0.047 tr|I3MEQ9|I3MEQ9_ICTTR Neuroendocrine protein 7B4 SCG5
SVSTVLTSKYR 0.067 0.117 0.093 0.141 1.829 0.871 0.117 0.932 tr|A0A287D1R9|A0A287D1R9_ICTTR Hemoglobin subunit alpha LOC101956162
SYSMEHF 0.035 0.071 0.027 0.060 1.578 0.658 0.060 1.223 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
SYSMEHFRWGKPV(-.98) 0.188 0.252 0.167 0.238 1.477 0.562 0.238 0.623 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
TLGGSQDKSLHN 0.001 0.004 0.000 0.001 4.597 2.201 0.001 3.066 tr|I3MPL6|I3MPL6_ICTTR Secretogranin-4 SCG3
TLYGFGG 0.984 0.984 1.000 1.000 1.062 0.087 0.984 0.007 tr|I3N7M0|I3N7M0_ICTTR Histone H4 LOC106145262
TNEIVEEQYTPQSL 0.057 0.104 0.059 0.098 1.868 0.901 0.098 1.010 tr|I3N6V4|I3N6V4_ICTTR Secretogranin-4 SCG2
TPLVTLFKNAIIKNAHKKGQ 0.171 0.240 0.200 0.272 2.089 1.063 0.240 0.620 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
VAELDQLLHY 0.731 0.781 0.888 0.898 1.103 0.142 0.781 0.107 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
VANALSHKYH 0.017 0.040 0.036 0.070 2.994 1.582 0.040 1.398 tr|A0A287CUK4|A0A287CUK4_ICTTR Hemoglobin subunit beta-S/F-like LOC101967841
VGRPEWWMDYQ 0.332 0.394 0.321 0.403 0.732 -0.450 0.394 0.404 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
VKMALQQEGFD 0.001 0.004 0.002 0.006 0.397 -1.335 0.004 2.423 tr|I3MEG8|I3MEG8_ICTTR Proprotein convertase subtilisin/kexin type 2 PCSK2
VKVGVNGF 0.027 0.059 0.046 0.079 1.819 0.863 0.059 1.227 tr|A0A287D887|A0A287D887_ICTTR Glyceraldehyde-3-phosphate dehydrogenase GAPDH
Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLFKNAIIKNAH 0.000 0.001 0.000 0.001 0.224 -2.159 0.001 3.141 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNA 0.000 0.000 0.000 0.001 0.071 -3.819 0.000 4.971 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAH 0.000 0.001 0.000 0.002 0.255 -1.974 0.001 2.848 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAHKKGQ 0.304 0.371 0.200 0.272 0.561 -0.833 0.272 0.566 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
YDRVAELDQLLHY 0.230 0.296 0.321 0.403 0.783 -0.353 0.296 0.529 tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
YGGFM(+15.99)RF 0.277 0.348 0.481 0.544 1.136 0.184 0.348 0.459 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
YGGFM(+15.99)RGL 0.681 0.744 0.370 0.446 0.815 -0.295 0.446 0.350 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
YGGFM(+15.99)TSEKSQTPLVTLFKNAIIKNAHKKGQ 0.946 0.966 0.673 0.727 1.365 0.449 0.727 0.138 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
YGGFMRF 0.038 0.074 0.021 0.047 1.673 0.743 0.047 1.325 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
YGGFMRGL 0.011 0.026 0.027 0.060 1.972 0.979 0.026 1.581 tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
YGGFMTSE 0.348 0.403 0.481 0.544 1.249 0.321 0.403 0.394 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
YGGFMTSEKSQTPLVTLFKNAIIKNA 0.004 0.013 0.006 0.015 0.490 -1.030 0.013 1.885 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
YGGFMTSEKSQTPLVTLFKNAIIKNAH 0.008 0.023 0.006 0.015 0.386 -1.374 0.015 1.817 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
YGGFMTSEKSQTPLVTLFKNAIIKNAHKKGQ 0.034 0.071 0.046 0.079 1.735 0.795 0.071 1.152 tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Code
# get DE features only tibble
uni_res_filt <- uni_res %>%
        dplyr::filter(BHT < FDR & BHW < FDR) %>%
        mutate(status = case_when(`FC(log2)` < - 0.6 ~ "Down",
                                  `FC(log2)` > 0.6 ~ "Up") )

if (nrow(uni_res_filt) == 0){
        print(paste("There is no significant differentiated features between",
                    temp_d$Label[2], "and", temp_d$Label[1], sep = " "))
} else {
        uni_res_filt = uni_res_filt %>%
                left_join(., feature_meta, by = c("variable" = "Peptide"))
        
        uni_res_filt %>% 
                knitr::kable(., digits = 3, "html") %>%
                kableExtra::kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive")) %>%
                kableExtra::scroll_box(width = "100%", height = "500px")
}
variable pT BHT pW BHW FC(lin) FC(log2) padj -log10padj status Accession Name GN
AGAPEPAEPMELAKPSA 0.001 0.006 0.002 0.006 0.248 -2.011 0.006 2.213 Down tr|I3MXH3|I3MXH3_ICTTR Vasopressin-neurophysin 2-copeptin-like AVP
EDREEASLQDRQYASHHT 0.007 0.021 0.021 0.047 3.147 1.654 0.021 1.677 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EDREEASLQDRQYASHHTTE 0.001 0.004 0.001 0.003 2.874 1.523 0.003 2.545 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EEASLQDRQYASHHT 0.000 0.002 0.001 0.003 3.938 1.978 0.002 2.680 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
EEASLQDRQYASHHTTE 0.000 0.003 0.001 0.003 2.415 1.272 0.003 2.545 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPE 0.011 0.027 0.011 0.029 2.349 1.232 0.027 1.563 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPESQ 0.006 0.018 0.001 0.004 2.518 1.332 0.004 2.355 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPESQRD 0.000 0.000 0.000 0.001 4.087 2.031 0.000 4.650 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FLGEAYHHVPESQRDKA 0.000 0.001 0.000 0.001 4.343 2.119 0.001 3.141 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
FPTLGGSQDKSLHN 0.000 0.000 0.000 0.001 2.250 1.170 0.000 5.140 Up tr|I3MPL6|I3MPL6_ICTTR Secretogranin-3 SCG3
GEAYHHVPESQRD 0.001 0.004 0.001 0.004 3.056 1.611 0.004 2.423 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
GEAYHHVPESQRDKA 0.001 0.004 0.000 0.001 4.852 2.279 0.001 3.066 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
GPEFRDDGAEPGPRE(-.98) 0.002 0.006 0.002 0.009 1.895 0.923 0.006 2.209 Up tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
KVVAGVANALSHKYH 0.006 0.018 0.004 0.012 1.886 0.916 0.012 1.936 Up tr|A0A287CUK4|A0A287CUK4_ICTTR Hemoglobin subunit beta-S/F-like LOC101967841
LAGAPEPA 0.000 0.001 0.000 0.001 0.043 -4.541 0.001 3.141 Down tr|I3MXH3|I3MXH3_ICTTR Vasopressin-neurophysin 2-copeptin-like AVP
LDQLLHY 0.000 0.000 0.000 0.001 4.580 2.195 0.000 3.902 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
LGEAYHHVPESQRD 0.000 0.002 0.000 0.001 5.558 2.475 0.001 3.066 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
MYEENSRDNPF 0.010 0.026 0.021 0.047 1.880 0.911 0.026 1.581 Up tr|I3N6V4|I3N6V4_ICTTR Secretogranin-3 SCG2
PEFRDDGAEPGPRE(-.98) 0.002 0.009 0.002 0.009 2.148 1.103 0.009 2.066 Up tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Q(-17.03)HWSYGLRPG(-.98) 0.001 0.005 0.006 0.015 2.119 1.084 0.005 2.268 Up tr|I3MCA0|I3MCA0_ICTTR Progonadoliberin-1 GNRH1
Q(-17.03)KIAEKFSQ 0.003 0.010 0.001 0.003 3.025 1.597 0.003 2.545 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
Q(-17.03)KIAEKFSQR(-.98) 0.001 0.004 0.000 0.001 5.746 2.523 0.001 2.890 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
RPVKVYPNGAEDES(+79.97)AESFPLEF 0.006 0.019 0.000 0.001 0.091 -3.466 0.001 3.066 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
RPVKVYPNGAEDESAESFPLEF 0.001 0.006 0.002 0.009 0.232 -2.109 0.006 2.213 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
RTLYGFGG 0.003 0.010 0.004 0.012 2.269 1.182 0.010 1.987 Up tr|I3N7M0|I3N7M0_ICTTR Histone H4 LOC106145262
SANSNPAMAPRE 0.000 0.002 0.001 0.003 3.701 1.888 0.002 2.796 Up tr|I3MP38|I3MP38_ICTTR Somatostatin SST
SLDSPAGPAE 0.003 0.010 0.004 0.012 3.484 1.801 0.010 1.987 Up tr|I3M5J1|I3M5J1_ICTTR Corticoliberin CRH
SPQLEDEAKEL 0.007 0.019 0.006 0.015 1.794 0.843 0.015 1.817 Up tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
SPQLEDEAKELQ 0.010 0.026 0.011 0.029 1.542 0.625 0.026 1.581 Up tr|I3MGR7|I3MGR7_ICTTR Proenkephalin-A PENK
SQGGEPGAYLTPDTREE 0.010 0.026 0.002 0.009 1.626 0.701 0.009 2.066 Up tr|I3NBA1|I3NBA1_ICTTR Secretogranin-1 CHGB
SVNPYLQGQRL 0.000 0.001 0.001 0.003 6.020 2.590 0.001 3.031 Up tr|I3MEQ9|I3MEQ9_ICTTR Neuroendocrine protein 7B2 SCG5
TLGGSQDKSLHN 0.001 0.004 0.000 0.001 4.597 2.201 0.001 3.066 Up tr|I3MPL6|I3MPL6_ICTTR Secretogranin-4 SCG3
VKMALQQEGFD 0.001 0.004 0.002 0.006 0.397 -1.335 0.004 2.423 Down tr|I3MEG8|I3MEG8_ICTTR Proprotein convertase subtilisin/kexin type 2 PCSK2
Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLFKNAIIKNAH 0.000 0.001 0.000 0.001 0.224 -2.159 0.001 3.141 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNA 0.000 0.000 0.000 0.001 0.071 -3.819 0.000 4.971 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAH 0.000 0.001 0.000 0.002 0.255 -1.974 0.001 2.848 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
YGGFMTSEKSQTPLVTLFKNAIIKNA 0.004 0.013 0.006 0.015 0.490 -1.030 0.013 1.885 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
YGGFMTSEKSQTPLVTLFKNAIIKNAH 0.008 0.023 0.006 0.015 0.386 -1.374 0.015 1.817 Down tr|I3M7B6|I3M7B6_ICTTR Pro-opiomelanocortin POMC
Code
# volcano plot
volcano_plot(uni_res_annotation, feature_col = "variable", fdr = FDR, log2fc = LOG2FC, save = FALSE)

Code
# heatmap
anno <- data.frame(Label = as.factor(t(d5)[, "class"])) %>%
        filter(Label %in% contrast ) %>%
        droplevels()

hm <- d5[-1, d5[1,] %in% contrast ] %>%
        rownames_to_column("variable") %>%
        mutate(across(-variable, as.numeric)) #%>%
        #left_join(., feature_meta, by = c("variable" = "Feature"))

# select color palette of current pair
selected_colors = anno_colors[levels(anno[[1]])]

heatmap(hm, feature_col = "variable", sample_anno = anno, sample_colors = selected_colors, feature_anno = feature_meta, feature_label = "Peptide", rowname_switch = TRUE, save = FALSE)